Restriction map for
pFN21AE6601
(Circular) (Six-base) MAPSORT of: pFN21AE6601.seq Check: 3522 from: 1 to: 5410
pFN21AE6601 5410 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2174 2174
Size: 5410
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2436 3220 278
Size: 53 83 186 936 900 784 2468
Fragments arranged by size:
2468 936 900 784 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2731 2731
Size: 5410
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2453 3473 4857 5125 1632
Size: 821 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1020 821 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 4309 5286 1203
Size: 3106 977 1327
Fragments arranged by size:
3106 1327 977
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 4139 4139
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1166 2351 1166
Size: 1185 4225
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2045 1184
Size: 861 4549
AlwNI CAG_nnn'CTG
Cuts at: 2110 2122 2152 3153 4725 2110
Size: 12 30 1001 1572 2795
Fragments arranged by size:
2795 1572 1001 30 12
ApaLI G'TGCA_C
Cuts at: 3467 4623 3467
Size: 1156 4254
ApoI r'AATT_y
Cuts at: 1493 2719 2945 3097 5206 1493
Size: 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2251 2411 2735 1870
Size: 73 308 160 324 4545
Fragments arranged by size:
4545 324 308 160 73
BamHI G'GATC_C
Cuts at: 2740 3107 2740
Size: 367 5043
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2360 2549 2657 2731
Size: 325 217 627 516 57 189 108 74
Cuts at: 2731 4186 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 516 325 217 189 108
74 57
BanII G_rGCy'C
Cuts at: 1391 2043 2559 2729 1391
Size: 652 516 170 4072
Fragments arranged by size:
4072 652 516 170
BbeI G_GCGC'C
Cuts at: 2307 2364 2553 2661 2307
Size: 57 189 108 5056
Fragments arranged by size:
5056 189 108 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3601 3635 1903
Size: 34 1664 34 3678
Fragments arranged by size:
3678 1664 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3302 4512 3302
Size: 1210 4200
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2332 2540 4021
Size: 107 122 71 631 605 660 208 1481
Cuts at: 4021 136
Size: 1525
Fragments arranged by size:
1525 1481 660 631 605 208 122 107
71
BglII A'GATC_T
Cuts at: 5405 5405
Size: 5410
BlpI GC'TnA_GC
Cuts at: 2390 2705 2827 2390
Size: 315 122 4973
Fragments arranged by size:
4973 315 122
Bme1580I G_kGCm'C
Cuts at: 1790 2517 3471 4627 1790
Size: 727 954 1156 2573
Fragments arranged by size:
2573 1156 954 727
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5410
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3141 4099 442
Size: 416 53 83 399 24 1724 958 1753
Fragments arranged by size:
1753 1724 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2474 4070 1239
Size: 1076 159 1596 2579
Fragments arranged by size:
2579 1596 1076 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4400 4698 3535
Size: 865 298 4247
Fragments arranged by size:
4247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4073 882
Size: 617 2574 2219
Fragments arranged by size:
2574 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3201 493
Size: 711 1997 2702
Fragments arranged by size:
2702 1997 711
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 2361 2433 2550
Size: 53 83 186 936 771 57 72 117
Cuts at: 2550 2658 3217 3599 4234 275
Size: 108 559 382 635 1451
Fragments arranged by size:
1451 936 771 635 559 382 186 117
108 83 72 57 53
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5185
Fragments arranged by size:
5185 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2356 2575 1186
Size: 844 326 219 4021
Fragments arranged by size:
4021 844 326 219
BseYI C'CCAG_C
Cuts at: 2299 4613 5109 2299
Size: 2314 496 2600
Fragments arranged by size:
2600 2314 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2303 1163
Size: 573 567 4270
Fragments arranged by size:
4270 573 567
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2197 2336 2592 2860 3621 4649
Size: 275 186 196 139 256 268 761 1028
Cuts at: 4649 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 275 268 256 196 186
139
BsiHKAI G_wGCw'C
Cuts at: 2396 2559 3471 3556 4627 2396
Size: 163 912 85 1071 3179
Fragments arranged by size:
3179 1071 912 163 85
BsmI GAATG_Cn'
Cuts at: 2921 3014 2921
Size: 93 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2396 2517 2559 2729 3471 3556
Size: 399 253 353 121 42 170 742 85
Cuts at: 3556 4627 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 253 170 121
85 42
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2118 2174 2274 2451 2765 844
Size: 1274 56 100 177 314 3489
Fragments arranged by size:
3489 1274 314 177 100 56
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 3297 1394
Size: 429 410 1064 3507
Fragments arranged by size:
3507 1064 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 3905 4079 65
Size: 1183 2657 174 1396
Fragments arranged by size:
2657 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2576 4054 4241 1700
Size: 110 766 1478 187 2869
Fragments arranged by size:
2869 1478 766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 2218 2289 2381 2383 2385 1925
Size: 293 71 92 2 2 4950
Fragments arranged by size:
4950 293 92 71 2 2
BssSI C'ACGA_G
Cuts at: 3470 4482 3470
Size: 1012 4398
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 2122 794
Size: 1316 12 4082
Fragments arranged by size:
4082 1316 12
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405
Size: 768 367 385 17 1452 86 12 346
Cuts at: 5405 1972
Size: 1977
Fragments arranged by size:
1977 1452 768 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2327 2565 513
Size: 552 939 323 238 3358
Fragments arranged by size:
3358 939 552 323 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4671
Fragments arranged by size:
4671 683 56
BtsI GCAGTG_nn'
Cuts at: 2926 3720 3740 2926
Size: 794 20 4596
Fragments arranged by size:
4596 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DraIII CAC_nnn'GTG
Cuts at: 2074 2074
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2432 4417 809
Size: 507 1116 1985 1802
Fragments arranged by size:
1985 1802 1116 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2205 2333 2640
Size: 54 1425 50 275 18 375 128 307
Cuts at: 2640 2857 3746 5375 8
Size: 217 889 1629 43
Fragments arranged by size:
1629 1425 889 375 307 275 217 128
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2333 2857 1537
Size: 275 521 524 4090
Fragments arranged by size:
4090 524 521 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3340 1353
Size: 1987 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 2432 3997 4371 4517 387
Size: 1064 138 522 321 1565 374 146 1280
Fragments arranged by size:
1565 1280 1064 522 374 321 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2315 2453 2474 3473 4070 4857 5125
Size: 393 683 138 21 999 597 787 268
Cuts at: 5125 1239
Size: 1524
Fragments arranged by size:
1524 999 787 683 597 393 268 138
21
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2114
Size: 5410
EcoO109I rG'GnC_Cy
Cuts at: 2200 2464 2635 2200
Size: 264 171 4975
Fragments arranged by size:
4975 264 171
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
FspI TGC'GCA
Cuts at: 2345 3916 2345
Size: 1571 3839
FspAI rTGC'GCAy
Cuts at: 2345 2345
Size: 5410
HaeII r_GCGC'y
Cuts at: 1678 2030 2307 2364 2553 2577 2661 4557 1678
Size: 352 277 57 189 24 84 1896 2531
Fragments arranged by size:
2531 1896 352 277 189 84 57 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 4051 4083 4125
Size: 32 135 32 974 32 1473 32 42
Cuts at: 4125 4157 1373
Size: 32 2626
Fragments arranged by size:
2626 1473 974 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2509 2754 3000 669
Size: 988 281 571 245 246 3079
Fragments arranged by size:
3079 988 571 281 246 245
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5410
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KasI G'GCGC_C
Cuts at: 2303 2360 2549 2657 2303
Size: 57 189 108 5056
Fragments arranged by size:
5056 189 108 57
KpnI G_GTAC'C
Cuts at: 2735 2735
Size: 5410
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4524 4708 1305
Size: 3219 184 2007
Fragments arranged by size:
3219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3588
Fragments arranged by size:
3588 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2351 2498 3368 3727 3886 518
Size: 648 1185 147 870 359 159 2042
Fragments arranged by size:
2042 1185 870 648 359 159 147
MspA1I CmG'CkG
Cuts at: 1606 2317 2329 2338 2520 2826 3503 4651 4896
Size: 711 12 9 182 306 677 1148 245
Cuts at: 4896 1606
Size: 2120
Fragments arranged by size:
2120 1148 711 677 306 245 182 12
9
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 5410
NarI GG'CG_CC
Cuts at: 2304 2361 2550 2658 2304
Size: 57 189 108 5056
Fragments arranged by size:
5056 189 108 57
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3919
Fragments arranged by size:
3919 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 5410
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2333 2857 2333
Size: 524 4886
NspI r_CATG'y
Cuts at: 1132 1229 2284 2768 4313 1132
Size: 97 1055 484 1545 2229
Fragments arranged by size:
2229 1545 1055 484 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5410
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2071 1108
Size: 963 4447
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2464 2464
Size: 5410
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PspXI vC'TCGA_Gb
Cuts at: 2411 2411
Size: 5410
PstI C_TGCA'G
Cuts at: 830 2762 830
Size: 1932 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5410
SacII CC_GC'GG
Cuts at: 2330 2330
Size: 5410
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SbfI CC_TGCA'GG
Cuts at: 2762 2762
Size: 5410
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SexAI A'CCwGG_T
Cuts at: 2070 2070
Size: 5410
SfcI C'TryA_G
Cuts at: 826 1045 2758 3893 4574 4765 826
Size: 219 1713 1135 681 191 1471
Fragments arranged by size:
1713 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2305 2362 2551 2659 2305
Size: 57 189 108 5056
Fragments arranged by size:
5056 189 108 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5410
SmaI CCC'GGG
Cuts at: 1872 2737 1872
Size: 865 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 3514 4415 4677 820
Size: 197 926 468 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 901 468 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5410
StyI C'CwwG_G
Cuts at: 513 1065 2004 2849 513
Size: 552 939 845 3074
Fragments arranged by size:
3074 939 845 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2230 2578 3437 3454 3607 3792 4237
Size: 1255 650 348 859 17 153 185 445
Cuts at: 4237 325
Size: 1498
Fragments arranged by size:
1498 1255 859 650 445 348 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 5368 96
Size: 274 80 33 51 1443 1679 1712 138
Fragments arranged by size:
1712 1679 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5370 5402 5370
Size: 32 5378
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2635 2635
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 2411 1943
Size: 468 4942
XmaI C'CCGG_G
Cuts at: 1870 2735 1870
Size: 865 4545
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2434 3218 276
Size: 53 83 186 936 900 784 2468
Fragments arranged by size:
2468 936 900 784 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV
FalI FseI NruI NsiI PacI PfoI PmlI PspOMI
PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI