Restriction map for
pFN21AE6593
(Circular) (Six-base) MAPSORT of: pFN21AE6593.seq Check: 2369 from: 1 to: 4858
pFN21AE6593 4858 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2668 278
Size: 53 83 186 936 1132 2468
Fragments arranged by size:
2468 1132 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2201 1937
Size: 264 4594
Acc65I G'GTAC_C
Cuts at: 2179 2179
Size: 4858
AclI AA'CG_TT
Cuts at: 1563 2985 3358 1563
Size: 1422 373 3063
Fragments arranged by size:
3063 1422 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2921 4305 4573 1632
Size: 473 816 1384 268 1917
Fragments arranged by size:
1917 1384 816 473 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4661
AflIII A'CryG_T
Cuts at: 1203 3757 4734 1203
Size: 2554 977 1327
Fragments arranged by size:
2554 1327 977
AgeI A'CCGG_T
Cuts at: 3689 3689
Size: 4858
AhdI GACnn_n'nnGTC
Cuts at: 3587 3587
Size: 4858
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4858
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4858
AlwNI CAG_nnn'CTG
Cuts at: 2601 4173 2601
Size: 1572 3286
ApaLI G'TGCA_C
Cuts at: 2915 4071 2915
Size: 1156 3702
ApoI r'AATT_y
Cuts at: 1493 2093 2167 2393 2545 4654 1493
Size: 600 74 226 152 2109 1697
Fragments arranged by size:
2109 1697 600 226 152 74
AseI AT'TA_AT
Cuts at: 160 1032 3412 160
Size: 872 2380 1606
Fragments arranged by size:
2380 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4858
AvaI C'yCGr_G
Cuts at: 1870 1943 2183 1870
Size: 73 240 4545
Fragments arranged by size:
4545 240 73
BamHI G'GATC_C
Cuts at: 2188 2555 2188
Size: 367 4491
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 3634 618
Size: 325 217 627 392 1455 1842
Fragments arranged by size:
1842 1455 627 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2177 1391
Size: 786 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 2120 928
Size: 971 194 27 3666
Fragments arranged by size:
3666 971 194 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3049 3083 1903
Size: 34 1112 34 3678
Fragments arranged by size:
3678 1112 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2750 3960 2750
Size: 1210 3648
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4858
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3469 136
Size: 107 122 71 631 605 337 1460 1525
Fragments arranged by size:
1525 1460 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2055 4853 2055
Size: 2798 2060
BlpI GC'TnA_GC
Cuts at: 2275 2275
Size: 4858
Bme1580I G_kGCm'C
Cuts at: 1790 2919 4075 1790
Size: 1129 1156 2573
Fragments arranged by size:
2573 1156 1129
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4858
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2589 3547 442
Size: 416 53 83 399 24 1172 958 1753
Fragments arranged by size:
1753 1172 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4858
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 3518 1239
Size: 799 1480 2579
Fragments arranged by size:
2579 1480 799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2983 3848 4146 2983
Size: 865 298 3695
Fragments arranged by size:
3695 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3521 882
Size: 617 2022 2219
Fragments arranged by size:
2219 2022 617
BsaAI yAC'GTr
Cuts at: 493 1204 2649 493
Size: 711 1445 2702
Fragments arranged by size:
2702 1445 711
BsaBI GATnn'nnATC
Cuts at: 4852 4852
Size: 4858
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2665 3047 3682 275
Size: 53 83 186 936 1132 382 635 1451
Fragments arranged by size:
1451 1132 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3291 3689 3963 4110 4521 3291
Size: 398 274 147 411 3628
Fragments arranged by size:
3628 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4633
Fragments arranged by size:
4633 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4858
BseYI C'CCAG_C
Cuts at: 4061 4557 4061
Size: 496 4362
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4285
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2308 3069 4097 1540
Size: 275 186 307 761 1028 2301
Fragments arranged by size:
2301 1028 761 307 275 186
BsiHKAI G_wGCw'C
Cuts at: 2919 3004 4075 2919
Size: 85 1071 3702
Fragments arranged by size:
3702 1071 85
BsmI GAATG_Cn'
Cuts at: 2369 2462 2369
Size: 93 4765
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2919 3004 4075 1391
Size: 399 387 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 85
BspEI T'CCGG_A
Cuts at: 4521 4521
Size: 4858
BspHI T'CATG_A
Cuts at: 2747 4477 2747
Size: 1730 3128
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2213 844
Size: 1369 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2745 1394
Size: 429 922 3507
Fragments arranged by size:
3507 922 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3353 3527 65
Size: 1183 2105 174 1396
Fragments arranged by size:
2105 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3502 3689 1700
Size: 110 1692 187 2869
Fragments arranged by size:
2869 1692 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4858
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4858
BssSI C'ACGA_G
Cuts at: 2918 3930 2918
Size: 1012 3846
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4858
BstBI TT'CG_AA
Cuts at: 3662 3662
Size: 4858
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4858
BstYI r'GATC_y
Cuts at: 1972 2055 2188 2555 2940 2957 4409 4495 4507
Size: 83 133 367 385 17 1452 86 12
Cuts at: 4507 4853 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 133 86 83
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3367
Fragments arranged by size:
3367 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4119
Fragments arranged by size:
4119 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2374 3168 3188 2076
Size: 298 794 20 3746
Fragments arranged by size:
3746 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4858
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4823
DraI TTT'AAA
Cuts at: 2162 2162
Size: 4858
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3865 809
Size: 507 2549 1802
Fragments arranged by size:
2549 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2305 3194 4823
Size: 54 1425 50 275 18 475 889 1629
Cuts at: 4823 8
Size: 43
Fragments arranged by size:
1629 1425 889 475 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2305 1537
Size: 275 493 4090
Fragments arranged by size:
4090 493 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2788 1353
Size: 1435 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3445 3819 3965 387
Size: 1064 138 1856 374 146 1280
Fragments arranged by size:
1856 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2105 2921 3518 4305 4573 1239
Size: 393 406 67 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 406 393 268 67
EcoRI G'AATT_C
Cuts at: 2093 2167 2545 2093
Size: 74 378 4406
Fragments arranged by size:
4406 378 74
FspI TGC'GCA
Cuts at: 3364 3364
Size: 4858
HaeII r_GCGC'y
Cuts at: 1678 4005 1678
Size: 2327 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3499 3531 3573 3605 1373
Size: 32 135 32 1927 32 42 32 2626
Fragments arranged by size:
2626 1927 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2202 2448 669
Size: 988 281 264 246 3079
Fragments arranged by size:
3079 988 281 264 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4858
HpaI GTT'AAC
Cuts at: 2448 2448
Size: 4858
KpnI G_GTAC'C
Cuts at: 2183 2183
Size: 4858
MfeI C'AATT_G
Cuts at: 2457 2457
Size: 4858
MluI A'CGCG_T
Cuts at: 4734 4734
Size: 4858
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3972 4156 1305
Size: 2667 184 2007
Fragments arranged by size:
2667 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3036
Fragments arranged by size:
3036 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2816 3175 3334 518
Size: 648 1650 359 159 2042
Fragments arranged by size:
2042 1650 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2274 2951 4099 4344 1606
Size: 668 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 668 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3367
Fragments arranged by size:
3367 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4858
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4858
NotI GC'GGCC_GC
Cuts at: 2305 2305
Size: 4858
NspI r_CATG'y
Cuts at: 1132 1229 2216 3761 1132
Size: 97 987 1545 2229
Fragments arranged by size:
2229 1545 987 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4858
PciI A'CATG_T
Cuts at: 3757 3757
Size: 4858
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4858
PmeI GTTT'AAAC
Cuts at: 2162 2162
Size: 4858
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2927 2959 4440 4472 2927
Size: 32 1481 32 3313
Fragments arranged by size:
3313 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4858
PsiI TTA'TAA
Cuts at: 2428 2428
Size: 4858
PstI C_TGCA'G
Cuts at: 830 2210 830
Size: 1380 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4858
SalI G'TCGA_C
Cuts at: 1936 2200 1936
Size: 264 4594
SbfI CC_TGCA'GG
Cuts at: 2210 2210
Size: 4858
ScaI AGT'ACT
Cuts at: 3106 4818 3106
Size: 1712 3146
SfcI C'TryA_G
Cuts at: 826 1045 2206 3341 4022 4213 826
Size: 219 1161 1135 681 191 1471
Fragments arranged by size:
1471 1161 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4858
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4858
SmaI CCC'GGG
Cuts at: 1872 2185 1872
Size: 313 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2962 3863 4125 820
Size: 197 926 1019 901 262 1553
Fragments arranged by size:
1553 1019 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2649 493
Size: 2156 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4858
SphI G_CATG'C
Cuts at: 1132 2216 1132
Size: 1084 3774
SspI AAT'ATT
Cuts at: 5 52 2782 5
Size: 47 2730 2081
Fragments arranged by size:
2730 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4858
StyI C'CwwG_G
Cuts at: 513 1065 2004 2297 513
Size: 552 939 293 3074
Fragments arranged by size:
3074 939 552 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2885 2902 3055 3240 3685 325
Size: 1255 1305 17 153 185 445 1498
Fragments arranged by size:
1498 1305 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3104 4816 96
Size: 274 80 33 51 1443 1127 1712 138
Fragments arranged by size:
1712 1443 1127 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4818 4850 4818
Size: 32 4826
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4858
XbaI T'CTAG_A
Cuts at: 2194 2194
Size: 4858
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4858
XmaI C'CCGG_G
Cuts at: 1870 2183 1870
Size: 313 4545
XmnI GAAnn'nnTTC
Cuts at: 2987 2987
Size: 4858
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2666 276
Size: 53 83 186 936 1132 2468
Fragments arranged by size:
2468 1132 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI