Restriction map for
pFN21AE6580
(Circular) (Six-base) MAPSORT of: pFN21AE6580.seq Check: 5028 from: 1 to: 5098
pFN21AE6580 5098 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2245 2245
Size: 5098
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2908 278
Size: 53 83 186 936 1372 2468
Fragments arranged by size:
2468 1372 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2441 1937
Size: 504 4594
Acc65I G'GTAC_C
Cuts at: 2419 2419
Size: 5098
AclI AA'CG_TT
Cuts at: 1563 3225 3598 1563
Size: 1662 373 3063
Fragments arranged by size:
3063 1662 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 3161 4545 4813 1632
Size: 608 921 1384 268 1917
Fragments arranged by size:
1917 1384 921 608 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4901
AflIII A'CryG_T
Cuts at: 1203 3997 4974 1203
Size: 2794 977 1327
Fragments arranged by size:
2794 1327 977
AgeI A'CCGG_T
Cuts at: 3929 3929
Size: 5098
AhdI GACnn_n'nnGTC
Cuts at: 3827 3827
Size: 5098
AleI CACnn'nnGTG
Cuts at: 1166 2039 2195 1166
Size: 873 156 4069
Fragments arranged by size:
4069 873 156
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5098
AlwNI CAG_nnn'CTG
Cuts at: 2134 2841 4413 2134
Size: 707 1572 2819
Fragments arranged by size:
2819 1572 707
ApaLI G'TGCA_C
Cuts at: 3155 4311 3155
Size: 1156 3942
ApoI r'AATT_y
Cuts at: 1493 2407 2633 2785 4894 1493
Size: 914 226 152 2109 1697
Fragments arranged by size:
2109 1697 914 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3652 160
Size: 872 2620 1606
Fragments arranged by size:
2620 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5098
AvaI C'yCGr_G
Cuts at: 1870 1943 2423 1870
Size: 73 480 4545
Fragments arranged by size:
4545 480 73
BamHI G'GATC_C
Cuts at: 2428 2795 2428
Size: 367 4731
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2419 3874 618
Size: 325 217 627 632 1455 1842
Fragments arranged by size:
1842 1455 632 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2417 1391
Size: 1026 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 928
Size: 971 307 3820
Fragments arranged by size:
3820 971 307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3289 3323 1903
Size: 34 1352 34 3678
Fragments arranged by size:
3678 1352 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2990 4200 2990
Size: 1210 3888
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5098
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3709 136
Size: 107 122 71 631 605 2037 1525
Fragments arranged by size:
2037 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5093 5093
Size: 5098
BlpI GC'TnA_GC
Cuts at: 2048 2515 2048
Size: 467 4631
Bme1580I G_kGCm'C
Cuts at: 1790 3159 4315 1790
Size: 1369 1156 2573
Fragments arranged by size:
2573 1369 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2829 3787 442
Size: 416 53 83 399 24 1412 958 1753
Fragments arranged by size:
1753 1412 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5098
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 5098
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3758 1239
Size: 2519 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3223 4088 4386 3223
Size: 865 298 3935
Fragments arranged by size:
3935 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2174 3761 882
Size: 617 675 1587 2219
Fragments arranged by size:
2219 1587 675 617
BsaAI yAC'GTr
Cuts at: 493 1204 2889 493
Size: 711 1685 2702
Fragments arranged by size:
2702 1685 711
BsaBI GATnn'nnATC
Cuts at: 5092 5092
Size: 5098
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2905 3287 3922 275
Size: 53 83 186 936 1372 382 635 1451
Fragments arranged by size:
1451 1372 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3531 3929 4203 4350 4761 3531
Size: 398 274 147 411 3868
Fragments arranged by size:
3868 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4873
Fragments arranged by size:
4873 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 2279 2384 1186
Size: 1006 87 105 3900
Fragments arranged by size:
3900 1006 105 87
BseYI C'CCAG_C
Cuts at: 2022 2146 4301 4797 2022
Size: 124 2155 496 2323
Fragments arranged by size:
2323 2155 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2548 3309 4337 1540
Size: 275 186 547 761 1028 2301
Fragments arranged by size:
2301 1028 761 547 275 186
BsiHKAI G_wGCw'C
Cuts at: 2367 3159 3244 4315 2367
Size: 792 85 1071 3150
Fragments arranged by size:
3150 1071 792 85
BsmI GAATG_Cn'
Cuts at: 2609 2702 2609
Size: 93 5005
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2417 3159 3244 4315 1391
Size: 399 577 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 399 85 50
BspEI T'CCGG_A
Cuts at: 4761 4761
Size: 5098
BspHI T'CATG_A
Cuts at: 2987 4717 2987
Size: 1730 3368
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2245 2453 844
Size: 1401 208 3489
Fragments arranged by size:
3489 1401 208
BsrBI CCG'CTC
Cuts at: 1394 1823 2985 1394
Size: 429 1162 3507
Fragments arranged by size:
3507 1162 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3593 3767 65
Size: 1183 2345 174 1396
Fragments arranged by size:
2345 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3742 3929 1700
Size: 110 1932 187 2869
Fragments arranged by size:
2869 1932 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5098
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5098
BssSI C'ACGA_G
Cuts at: 2366 3158 4170 2366
Size: 792 1012 3294
Fragments arranged by size:
3294 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5098
BstBI TT'CG_AA
Cuts at: 3902 3902
Size: 5098
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5098
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5098
BstYI r'GATC_y
Cuts at: 1972 2428 2795 3180 3197 4649 4735 4747 5093
Size: 456 367 385 17 1452 86 12 346
Cuts at: 5093 1972
Size: 1977
Fragments arranged by size:
1977 1452 456 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4546
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4359
Fragments arranged by size:
4359 683 56
BtsI GCAGTG_nn'
Cuts at: 2614 3408 3428 2614
Size: 794 20 4284
Fragments arranged by size:
4284 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5098
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5063
DraI TTT'AAA
Cuts at: 2402 2402
Size: 5098
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4105 809
Size: 507 2789 1802
Fragments arranged by size:
2789 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2545 3434 5063
Size: 54 1425 50 275 18 715 889 1629
Cuts at: 5063 8
Size: 43
Fragments arranged by size:
1629 1425 889 715 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2545 1537
Size: 275 733 4090
Fragments arranged by size:
4090 733 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3028 1353
Size: 1675 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3685 4059 4205 387
Size: 1064 138 2096 374 146 1280
Fragments arranged by size:
2096 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 3161 3758 4545 4813 1239
Size: 393 608 921 597 787 268 1524
Fragments arranged by size:
1524 921 787 608 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2097 2161 2097
Size: 64 5034
EcoRI G'AATT_C
Cuts at: 2407 2785 2407
Size: 378 4720
FspI TGC'GCA
Cuts at: 3604 3604
Size: 5098
HaeII r_GCGC'y
Cuts at: 1678 2349 4245 1678
Size: 671 1896 2531
Fragments arranged by size:
2531 1896 671
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2366 2398 3739 3771 3813
Size: 32 135 32 794 32 1341 32 42
Cuts at: 3813 3845 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 794 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2442 2688 669
Size: 988 281 504 246 3079
Fragments arranged by size:
3079 988 504 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5098
HpaI GTT'AAC
Cuts at: 2688 2688
Size: 5098
KpnI G_GTAC'C
Cuts at: 2423 2423
Size: 5098
MfeI C'AATT_G
Cuts at: 2697 2697
Size: 5098
MluI A'CGCG_T
Cuts at: 4974 4974
Size: 5098
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4212 4396 1305
Size: 2907 184 2007
Fragments arranged by size:
2907 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3276
Fragments arranged by size:
3276 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2195 3056 3415 3574 518
Size: 648 873 156 861 359 159 2042
Fragments arranged by size:
2042 873 861 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 2026 2067 2514 3191 4339 4584 1606
Size: 420 41 447 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 447 420 245 41
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4546
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5098
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5098
NotI GC'GGCC_GC
Cuts at: 2545 2545
Size: 5098
NspI r_CATG'y
Cuts at: 1132 1229 2456 4001 1132
Size: 97 1227 1545 2229
Fragments arranged by size:
2229 1545 1227 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5098
PciI A'CATG_T
Cuts at: 3997 3997
Size: 5098
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2029 2134 1108
Size: 921 105 4072
Fragments arranged by size:
4072 921 105
PmeI GTTT'AAAC
Cuts at: 2402 2402
Size: 5098
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 4680 4712 3167
Size: 32 1481 32 3553
Fragments arranged by size:
3553 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2097
Size: 5098
PshAI GACnn'nnGTC
Cuts at: 1613 2015 1613
Size: 402 4696
PsiI TTA'TAA
Cuts at: 2668 2668
Size: 5098
PstI C_TGCA'G
Cuts at: 830 2450 830
Size: 1620 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5098
PvuII CAG'CTG
Cuts at: 2026 2067 2026
Size: 41 5057
SalI G'TCGA_C
Cuts at: 1936 2440 1936
Size: 504 4594
SanDI GG'GwC_CC
Cuts at: 2097 2097
Size: 5098
SbfI CC_TGCA'GG
Cuts at: 2450 2450
Size: 5098
ScaI AGT'ACT
Cuts at: 3346 5058 3346
Size: 1712 3386
SfcI C'TryA_G
Cuts at: 826 1045 2446 3581 4262 4453 826
Size: 219 1401 1135 681 191 1471
Fragments arranged by size:
1471 1401 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5098
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5098
SmaI CCC'GGG
Cuts at: 1872 2425 1872
Size: 553 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3202 4103 4365 820
Size: 197 926 1259 901 262 1553
Fragments arranged by size:
1553 1259 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2889 493
Size: 2396 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5098
SphI G_CATG'C
Cuts at: 1132 2456 1132
Size: 1324 3774
SspI AAT'ATT
Cuts at: 5 52 2242 3022 5
Size: 47 2190 780 2081
Fragments arranged by size:
2190 2081 780 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5098
StyI C'CwwG_G
Cuts at: 513 1065 2537 513
Size: 552 1472 3074
Fragments arranged by size:
3074 1472 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 2404 3125 3142 3295 3480 3925
Size: 1255 456 368 721 17 153 185 445
Cuts at: 3925 325
Size: 1498
Fragments arranged by size:
1498 1255 721 456 445 368 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3344 5056 96
Size: 274 80 33 51 1443 1367 1712 138
Fragments arranged by size:
1712 1443 1367 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5058 5090 5058
Size: 32 5066
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5098
XbaI T'CTAG_A
Cuts at: 2434 2434
Size: 5098
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5098
XmaI C'CCGG_G
Cuts at: 1870 2423 1870
Size: 553 4545
XmnI GAAnn'nnTTC
Cuts at: 3227 3227
Size: 5098
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2906 276
Size: 53 83 186 936 1372 2468
Fragments arranged by size:
2468 1372 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SfoI
SrfI SwaI XcmI