Restriction map for
pFN21AE6569
(Circular) (Six-base) MAPSORT of: pFN21AE6569.seq Check: 6139 from: 1 to: 5344
pFN21AE6569 5344 bp
With 182 enzymes: SgfI *
December 25, 2014 18:02 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3154 278
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2687 1937
Size: 750 4594
Acc65I G'GTAC_C
Cuts at: 2665 2665
Size: 5344
AclI AA'CG_TT
Cuts at: 1563 3471 3844 1563
Size: 1908 373 3063
Fragments arranged by size:
3063 1908 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3407 4791 5059 1632
Size: 1775 1384 268 1917
Fragments arranged by size:
1917 1775 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5147
AflIII A'CryG_T
Cuts at: 1203 2226 4243 5220 1203
Size: 1023 2017 977 1327
Fragments arranged by size:
2017 1327 1023 977
AgeI A'CCGG_T
Cuts at: 4175 4175
Size: 5344
AhdI GACnn_n'nnGTC
Cuts at: 4073 4073
Size: 5344
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5344
AlwNI CAG_nnn'CTG
Cuts at: 2443 3087 4659 2443
Size: 644 1572 3128
Fragments arranged by size:
3128 1572 644
ApaI G_GGCC'C
Cuts at: 2394 2394
Size: 5344
ApaLI G'TGCA_C
Cuts at: 3401 4557 3401
Size: 1156 4188
ApoI r'AATT_y
Cuts at: 1493 2653 2879 3031 5140 1493
Size: 1160 226 152 2109 1697
Fragments arranged by size:
2109 1697 1160 226 152
AscI GG'CGCG_CC
Cuts at: 2339 2339
Size: 5344
AseI AT'TA_AT
Cuts at: 160 1032 3898 160
Size: 872 2866 1606
Fragments arranged by size:
2866 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
AvaI C'yCGr_G
Cuts at: 1870 1943 2117 2135 2669 1870
Size: 73 174 18 534 4545
Fragments arranged by size:
4545 534 174 73 18
BamHI G'GATC_C
Cuts at: 2674 3041 2674
Size: 367 4977
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2461 2665 4120 618
Size: 325 217 627 674 204 1455 1842
Fragments arranged by size:
1842 1455 674 627 325 217 204
BanII G_rGCy'C
Cuts at: 1391 2124 2394 2541 2663 1391
Size: 733 270 147 122 4072
Fragments arranged by size:
4072 733 270 147 122
BbeI G_GCGC'C
Cuts at: 2465 2465
Size: 5344
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3535 3569 1903
Size: 34 50 34 1514 34 3678
Fragments arranged by size:
3678 1514 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3236 4446 3236
Size: 1210 4134
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5344
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2331 2621 3955
Size: 107 122 71 631 605 659 290 1334
Cuts at: 3955 136
Size: 1525
Fragments arranged by size:
1525 1334 659 631 605 290 122 107
71
BglII A'GATC_T
Cuts at: 5339 5339
Size: 5344
BlpI GC'TnA_GC
Cuts at: 2761 2761
Size: 5344
Bme1580I G_kGCm'C
Cuts at: 1790 2394 3405 4561 1790
Size: 604 1011 1156 2573
Fragments arranged by size:
2573 1156 1011 604
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4435
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3075 4033 442
Size: 416 53 83 399 24 1658 958 1753
Fragments arranged by size:
1753 1658 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2651 4004 1239
Size: 935 477 1353 2579
Fragments arranged by size:
2579 1353 935 477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2525 3469 4334 4632 2525
Size: 944 865 298 3237
Fragments arranged by size:
3237 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2210 4007 882
Size: 617 711 1797 2219
Fragments arranged by size:
2219 1797 711 617
BsaAI yAC'GTr
Cuts at: 493 1204 2269 3135 493
Size: 711 1065 866 2702
Fragments arranged by size:
2702 1065 866 711
BsaBI GATnn'nnATC
Cuts at: 5338 5338
Size: 5344
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2462 3151 3533 4168
Size: 53 83 186 936 929 689 382 635
Cuts at: 4168 275
Size: 1451
Fragments arranged by size:
1451 936 929 689 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2146 3777 4175 4449 4596 5007 2146
Size: 1631 398 274 147 411 2483
Fragments arranged by size:
2483 1631 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5119
Fragments arranged by size:
5119 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5344
BseYI C'CCAG_C
Cuts at: 4547 5043 4547
Size: 496 4848
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2237 2456 1163
Size: 573 501 219 4051
Fragments arranged by size:
4051 573 501 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2365 2416 2570 2794 3555 4583
Size: 275 186 364 51 154 224 761 1028
Cuts at: 4583 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 364 275 224 186 154
51
BsiHKAI G_wGCw'C
Cuts at: 2124 2321 2541 3405 3490 4561 2124
Size: 197 220 864 85 1071 2907
Fragments arranged by size:
2907 1071 864 220 197 85
BsmI GAATG_Cn'
Cuts at: 2855 2948 2855
Size: 93 5251
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2321 2394 2541 2663 3405 3490
Size: 399 334 197 73 147 122 742 85
Cuts at: 3490 4561 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 334 197 147 122
85 73
BspEI T'CCGG_A
Cuts at: 5007 5007
Size: 5344
BspHI T'CATG_A
Cuts at: 3233 4963 3233
Size: 1730 3614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2699 844
Size: 1855 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2564 3231 1394
Size: 429 741 667 3507
Fragments arranged by size:
3507 741 667 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3839 4013 65
Size: 1183 2591 174 1396
Fragments arranged by size:
2591 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2299 3988 4175 1700
Size: 110 489 1689 187 2869
Fragments arranged by size:
2869 1689 489 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5344
BssHII G'CGCG_C
Cuts at: 1925 2339 2424 2467 2469 2471 1925
Size: 414 85 43 2 2 4798
Fragments arranged by size:
4798 414 85 43 2 2
BssSI C'ACGA_G
Cuts at: 2494 3404 4416 2494
Size: 910 1012 3422
Fragments arranged by size:
3422 1012 910
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 794
Size: 1640 3704
BstBI TT'CG_AA
Cuts at: 4148 4148
Size: 5344
BstEII G'GTnAC_C
Cuts at: 1184 2302 1184
Size: 1118 4226
BstYI r'GATC_y
Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339
Size: 702 367 385 17 1452 86 12 346
Cuts at: 5339 1972
Size: 1977
Fragments arranged by size:
1977 1452 702 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2103 2365 2588 513
Size: 552 1038 262 223 3269
Fragments arranged by size:
3269 1038 552 262 223
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4605
Fragments arranged by size:
4605 683 56
BtsI GCAGTG_nn'
Cuts at: 2860 3654 3674 2860
Size: 794 20 4530
Fragments arranged by size:
4530 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5344
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5309
DraI TTT'AAA
Cuts at: 2648 2648
Size: 5344
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4351 809
Size: 507 3035 1802
Fragments arranged by size:
3035 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2323 2362 2567
Size: 54 1425 50 275 18 493 39 205
Cuts at: 2567 2791 3680 5309 8
Size: 224 889 1629 43
Fragments arranged by size:
1629 1425 889 493 275 224 205 54
50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2362 2567 2791 1537
Size: 275 550 205 224 4090
Fragments arranged by size:
4090 550 275 224 205
EarI CTCTTCn'nnn_
Cuts at: 1353 3274 1353
Size: 1921 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2195 3931 4305 4451 387
Size: 1064 138 606 1736 374 146 1280
Fragments arranged by size:
1736 1280 1064 606 374 146 138
EcoICRI GAG'CTC
Cuts at: 2122 2539 2122
Size: 417 4927
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2651 3407 4004 4791 5059 1239
Size: 393 542 477 756 597 787 268 1524
Fragments arranged by size:
1524 787 756 597 542 477 393 268
EcoRI G'AATT_C
Cuts at: 2653 3031 2653
Size: 378 4966
FspI TGC'GCA
Cuts at: 3850 3850
Size: 5344
HaeII r_GCGC'y
Cuts at: 1678 2295 2465 2512 2610 4491 1678
Size: 617 170 47 98 1881 2531
Fragments arranged by size:
2531 1881 617 170 98 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373
Size: 32 135 32 2413 32 42 32 2626
Fragments arranged by size:
2626 2413 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2688 2934 669
Size: 988 281 750 246 3079
Fragments arranged by size:
3079 988 750 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5344
HpaI GTT'AAC
Cuts at: 2934 2934
Size: 5344
KasI G'GCGC_C
Cuts at: 2461 2461
Size: 5344
KpnI G_GTAC'C
Cuts at: 2669 2669
Size: 5344
MfeI C'AATT_G
Cuts at: 2943 2943
Size: 5344
MluI A'CGCG_T
Cuts at: 5220 5220
Size: 5344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 4458 4642 1305
Size: 1209 1944 184 2007
Fragments arranged by size:
2007 1944 1209 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3522
Fragments arranged by size:
3522 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3302 3661 3820 518
Size: 648 2136 359 159 2042
Fragments arranged by size:
2136 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2241 2367 2403 2440 2590 2760 3437 4585
Size: 635 126 36 37 150 170 677 1148
Cuts at: 4585 4830 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 635 245 170 150 126
37 36
NarI GG'CG_CC
Cuts at: 2462 2462
Size: 5344
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4792
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5344
NotI GC'GGCC_GC
Cuts at: 2791 2791
Size: 5344
NspI r_CATG'y
Cuts at: 1132 1229 2702 4247 1132
Size: 97 1473 1545 2229
Fragments arranged by size:
2229 1545 1473 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5344
PciI A'CATG_T
Cuts at: 4243 4243
Size: 5344
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5344
PmeI GTTT'AAAC
Cuts at: 2648 2648
Size: 5344
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 4926 4958 3413
Size: 32 1481 32 3799
Fragments arranged by size:
3799 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5344
PsiI TTA'TAA
Cuts at: 2914 2914
Size: 5344
PspOMI G'GGCC_C
Cuts at: 2390 2390
Size: 5344
PstI C_TGCA'G
Cuts at: 830 2696 830
Size: 1866 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5344
PvuII CAG'CTG
Cuts at: 2241 2440 2241
Size: 199 5145
SacI G_AGCT'C
Cuts at: 2124 2541 2124
Size: 417 4927
SacII CC_GC'GG
Cuts at: 2368 2591 2368
Size: 223 5121
SalI G'TCGA_C
Cuts at: 1936 2686 1936
Size: 750 4594
SbfI CC_TGCA'GG
Cuts at: 2696 2696
Size: 5344
ScaI AGT'ACT
Cuts at: 3592 5304 3592
Size: 1712 3632
SfcI C'TryA_G
Cuts at: 826 1045 2692 3827 4508 4699 826
Size: 219 1647 1135 681 191 1471
Fragments arranged by size:
1647 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2331 2331
Size: 5344
SfoI GGC'GCC
Cuts at: 2463 2463
Size: 5344
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5344
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5344
SmaI CCC'GGG
Cuts at: 1872 2671 1872
Size: 799 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2540 3448 4349 4611 820
Size: 197 926 597 908 901 262 1553
Fragments arranged by size:
1553 926 908 901 597 262 197
SnaBI TAC'GTA
Cuts at: 493 3135 493
Size: 2642 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5344
SphI G_CATG'C
Cuts at: 1132 2702 1132
Size: 1570 3774
SspI AAT'ATT
Cuts at: 5 52 3268 5
Size: 47 3216 2081
Fragments arranged by size:
3216 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5344
StyI C'CwwG_G
Cuts at: 513 1065 2783 513
Size: 552 1718 3074
Fragments arranged by size:
3074 1718 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2288 2570 3371 3388 3541 3726 4171
Size: 1255 708 282 801 17 153 185 445
Cuts at: 4171 325
Size: 1498
Fragments arranged by size:
1498 1255 801 708 445 282 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 3590 5302
Size: 274 80 33 51 1443 258 1355 1712
Cuts at: 5302 96
Size: 138
Fragments arranged by size:
1712 1443 1355 274 258 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5304 5336 5304
Size: 32 5312
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5344
XbaI T'CTAG_A
Cuts at: 2680 2680
Size: 5344
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5344
XmaI C'CCGG_G
Cuts at: 1870 2669 1870
Size: 799 4545
XmnI GAAnn'nnTTC
Cuts at: 3473 3473
Size: 5344
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3152 276
Size: 53 83 186 936 1618 2468
Fragments arranged by size:
2468 1618 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SanDI SapI SexAI SrfI SwaI XcmI