Restriction map for
pFN21AE6568
(Circular) (Six-base) MAPSORT of: pFN21AE6568.seq Check: 5730 from: 1 to: 5269
pFN21AE6568 5269 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2271 3079 278
Size: 53 83 186 936 735 808 2468
Fragments arranged by size:
2468 936 808 735 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2612 1937
Size: 675 4594
Acc65I G'GTAC_C
Cuts at: 2590 2590
Size: 5269
AclI AA'CG_TT
Cuts at: 1563 3396 3769 1563
Size: 1833 373 3063
Fragments arranged by size:
3063 1833 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2065 3332 4716 4984 1632
Size: 433 1267 1384 268 1917
Fragments arranged by size:
1917 1384 1267 433 268
AfeI AGC'GCT
Cuts at: 2291 2291
Size: 5269
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5072
AflIII A'CryG_T
Cuts at: 1203 4168 5145 1203
Size: 2965 977 1327
Fragments arranged by size:
2965 1327 977
AgeI A'CCGG_T
Cuts at: 4100 4100
Size: 5269
AhdI GACnn_n'nnGTC
Cuts at: 3998 3998
Size: 5269
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5269
AlwNI CAG_nnn'CTG
Cuts at: 3012 4584 3012
Size: 1572 3697
ApaLI G'TGCA_C
Cuts at: 3326 4482 3326
Size: 1156 4113
ApoI r'AATT_y
Cuts at: 1493 2578 2804 2956 5065 1493
Size: 1085 226 152 2109 1697
Fragments arranged by size:
2109 1697 1085 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3823 160
Size: 872 2791 1606
Fragments arranged by size:
2791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2594 1870
Size: 73 107 544 4545
Fragments arranged by size:
4545 544 107 73
BamHI G'GATC_C
Cuts at: 2599 2966 2599
Size: 367 4902
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2590 4045 618
Size: 325 217 627 803 1455 1842
Fragments arranged by size:
1842 1455 803 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2588 1391
Size: 1197 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2048 2082 3460 3494 1903
Size: 34 111 34 1378 34 3678
Fragments arranged by size:
3678 1378 111 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3161 4371 3161
Size: 1210 4059
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5269
BfrBI ATG'CAT
Cuts at: 2414 2414
Size: 5269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3880 136
Size: 107 122 71 631 605 2208 1525
Fragments arranged by size:
2208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5264 5264
Size: 5269
BlpI GC'TnA_GC
Cuts at: 2686 2686
Size: 5269
Bme1580I G_kGCm'C
Cuts at: 1790 3330 4486 1790
Size: 1540 1156 2573
Fragments arranged by size:
2573 1540 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5269
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3000 3958 442
Size: 416 53 83 399 24 1583 958 1753
Fragments arranged by size:
1753 1583 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 3929 1239
Size: 1229 1461 2579
Fragments arranged by size:
2579 1461 1229
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 3394 4259 4557 2420
Size: 974 865 298 3132
Fragments arranged by size:
3132 974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3932 882
Size: 617 2433 2219
Fragments arranged by size:
2433 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3060 493
Size: 711 1856 2702
Fragments arranged by size:
2702 1856 711
BsaBI GATnn'nnATC
Cuts at: 2405 5263 2405
Size: 2858 2411
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2268 3076 3458 4093
Size: 53 83 186 936 735 808 382 635
Cuts at: 4093 275
Size: 1451
Fragments arranged by size:
1451 936 808 735 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3702 4100 4374 4521 4932 3702
Size: 398 274 147 411 4039
Fragments arranged by size:
4039 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2202 2232 2349 2379 1178
Size: 30 165 30 799 30 117 30 4068
Fragments arranged by size:
4068 799 165 117 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2370 1186
Size: 1184 4085
BseYI C'CCAG_C
Cuts at: 4472 4968 4472
Size: 496 4773
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4696
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2062 2075 2719 3480 4508 1540
Size: 275 186 61 13 644 761 1028 2301
Fragments arranged by size:
2301 1028 761 644 275 186 61 13
BsiHKAI G_wGCw'C
Cuts at: 3330 3415 4486 3330
Size: 85 1071 4113
Fragments arranged by size:
4113 1071 85
BsmI GAATG_Cn'
Cuts at: 2780 2873 2780
Size: 93 5176
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2588 3330 3415 4486 1391
Size: 399 798 742 85 1071 2174
Fragments arranged by size:
2174 1071 798 742 399 85
BspEI T'CCGG_A
Cuts at: 4932 4932
Size: 5269
BspHI T'CATG_A
Cuts at: 3158 4888 3158
Size: 1730 3539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2130 2624 844
Size: 1286 494 3489
Fragments arranged by size:
3489 1286 494
BsrBI CCG'CTC
Cuts at: 1394 1823 3156 1394
Size: 429 1333 3507
Fragments arranged by size:
3507 1333 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3764 3938 65
Size: 1183 2516 174 1396
Fragments arranged by size:
2516 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3913 4100 1700
Size: 110 2103 187 2869
Fragments arranged by size:
2869 2103 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5269
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5269
BssSI C'ACGA_G
Cuts at: 3329 4341 3329
Size: 1012 4257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5269
BstBI TT'CG_AA
Cuts at: 4073 4073
Size: 5269
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5269
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 5269
BstYI r'GATC_y
Cuts at: 1972 2599 2966 3351 3368 4820 4906 4918 5264
Size: 627 367 385 17 1452 86 12 346
Cuts at: 5264 1972
Size: 1977
Fragments arranged by size:
1977 1452 627 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3778
Fragments arranged by size:
3778 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4530
Fragments arranged by size:
4530 683 56
BtsI GCAGTG_nn'
Cuts at: 2785 3579 3599 2785
Size: 794 20 4455
Fragments arranged by size:
4455 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5234
DraI TTT'AAA
Cuts at: 2573 2573
Size: 5269
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4276 809
Size: 507 2960 1802
Fragments arranged by size:
2960 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2072 2716 3605
Size: 54 1425 50 275 18 242 644 889
Cuts at: 3605 5234 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 644 275 242 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2072 2716 1537
Size: 275 260 644 4090
Fragments arranged by size:
4090 644 275 260
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 3199 1353
Size: 1118 728 3423
Fragments arranged by size:
3423 1118 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3856 4230 4376 387
Size: 1064 138 2267 374 146 1280
Fragments arranged by size:
2267 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 2468 3332 3929 4716 4984 1239
Size: 393 433 403 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 597 433 403 393 268
EcoRI G'AATT_C
Cuts at: 2578 2956 2578
Size: 378 4891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2288 2288
Size: 5269
FspI TGC'GCA
Cuts at: 3775 3775
Size: 5269
HaeII r_GCGC'y
Cuts at: 1678 2293 4416 1678
Size: 615 2123 2531
Fragments arranged by size:
2531 2123 615
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2381 3910 3942 3984
Size: 32 135 32 777 32 1529 32 42
Cuts at: 3984 4016 1373
Size: 32 2626
Fragments arranged by size:
2626 1529 777 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2613 2859 669
Size: 988 281 675 246 3079
Fragments arranged by size:
3079 988 675 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5269
HpaI GTT'AAC
Cuts at: 2859 2859
Size: 5269
KpnI G_GTAC'C
Cuts at: 2594 2594
Size: 5269
MfeI C'AATT_G
Cuts at: 2868 2868
Size: 5269
MluI A'CGCG_T
Cuts at: 5145 5145
Size: 5269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4383 4567 1305
Size: 3078 184 2007
Fragments arranged by size:
3078 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3447
Fragments arranged by size:
3447 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 3586 3745 518
Size: 648 2061 359 159 2042
Fragments arranged by size:
2061 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2685 3362 4510 4755 1606
Size: 1079 677 1148 245 2120
Fragments arranged by size:
2120 1148 1079 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3778
Fragments arranged by size:
3778 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5269
NotI GC'GGCC_GC
Cuts at: 2716 2716
Size: 5269
NsiI A_TGCA'T
Cuts at: 2416 2416
Size: 5269
NspI r_CATG'y
Cuts at: 1132 1229 2627 4172 1132
Size: 97 1398 1545 2229
Fragments arranged by size:
2229 1545 1398 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5269
PciI A'CATG_T
Cuts at: 4168 4168
Size: 5269
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5269
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 5269
PmeI GTTT'AAAC
Cuts at: 2573 2573
Size: 5269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3338 3370 4851 4883 3338
Size: 32 1481 32 3724
Fragments arranged by size:
3724 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5269
PsiI TTA'TAA
Cuts at: 2839 2839
Size: 5269
PstI C_TGCA'G
Cuts at: 830 2621 830
Size: 1791 3478
PvuI CG_AT'CG
Cuts at: 2001 2062 2001
Size: 61 5208
SalI G'TCGA_C
Cuts at: 1936 2611 1936
Size: 675 4594
SbfI CC_TGCA'GG
Cuts at: 2621 2621
Size: 5269
ScaI AGT'ACT
Cuts at: 3517 5229 3517
Size: 1712 3557
SexAI A'CCwGG_T
Cuts at: 2145 2145
Size: 5269
SfcI C'TryA_G
Cuts at: 826 1045 2617 3752 4433 4624 826
Size: 219 1572 1135 681 191 1471
Fragments arranged by size:
1572 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5269
SmaI CCC'GGG
Cuts at: 1872 2052 2596 1872
Size: 180 544 4545
Fragments arranged by size:
4545 544 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2435 3373 4274 4536 820
Size: 197 926 492 938 901 262 1553
Fragments arranged by size:
1553 938 926 901 492 262 197
SnaBI TAC'GTA
Cuts at: 493 3060 493
Size: 2567 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5269
SphI G_CATG'C
Cuts at: 1132 2627 1132
Size: 1495 3774
SspI AAT'ATT
Cuts at: 5 52 3193 5
Size: 47 3141 2081
Fragments arranged by size:
3141 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2708 513
Size: 552 939 704 3074
Fragments arranged by size:
3074 939 704 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3296 3313 3466 3651 4096 325
Size: 1255 1716 17 153 185 445 1498
Fragments arranged by size:
1716 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3515 5227 96
Size: 274 80 33 51 1443 1538 1712 138
Fragments arranged by size:
1712 1538 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2205 2234 2237 5229 5261 2202
Size: 3 29 3 2992 32 2210
Fragments arranged by size:
2992 2210 32 29 3 3
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5269
XbaI T'CTAG_A
Cuts at: 2605 2605
Size: 5269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2031 2146 2031
Size: 115 5154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5269
XmaI C'CCGG_G
Cuts at: 1870 2050 2594 1870
Size: 180 544 4545
Fragments arranged by size:
4545 544 180
XmnI GAAnn'nnTTC
Cuts at: 3398 3398
Size: 5269
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2269 3077 276
Size: 53 83 186 936 735 808 2468
Fragments arranged by size:
2468 936 808 735 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI