Restriction map for
pFN21AE6564
(Circular) (Six-base) MAPSORT of: pFN21AE6564.seq Check: 2443 from: 1 to: 5245
pFN21AE6564 5245 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3055 278
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2588 1937
Size: 651 4594
Acc65I G'GTAC_C
Cuts at: 2566 2566
Size: 5245
AclI AA'CG_TT
Cuts at: 1563 3372 3745 1563
Size: 1809 373 3063
Fragments arranged by size:
3063 1809 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2123 2174 3308 4692 4960 1632
Size: 491 51 1134 1384 268 1917
Fragments arranged by size:
1917 1384 1134 491 268 51
AflII C'TTAA_G
Cuts at: 820 1017 2489 820
Size: 197 1472 3576
Fragments arranged by size:
3576 1472 197
AflIII A'CryG_T
Cuts at: 1203 4144 5121 1203
Size: 2941 977 1327
Fragments arranged by size:
2941 1327 977
AgeI A'CCGG_T
Cuts at: 4076 4076
Size: 5245
AhdI GACnn_n'nnGTC
Cuts at: 3974 3974
Size: 5245
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5245
AlwNI CAG_nnn'CTG
Cuts at: 2988 4560 2988
Size: 1572 3673
ApaLI G'TGCA_C
Cuts at: 3302 4458 3302
Size: 1156 4089
ApoI r'AATT_y
Cuts at: 1493 2554 2780 2932 5041 1493
Size: 1061 226 152 2109 1697
Fragments arranged by size:
2109 1697 1061 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3799 160
Size: 872 2767 1606
Fragments arranged by size:
2767 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
AvaI C'yCGr_G
Cuts at: 1870 1943 2202 2570 1870
Size: 73 259 368 4545
Fragments arranged by size:
4545 368 259 73
BamHI G'GATC_C
Cuts at: 2040 2575 2942 2040
Size: 535 367 4343
Fragments arranged by size:
4343 535 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2509 2566 4021 618
Size: 325 217 627 722 57 1455 1842
Fragments arranged by size:
1842 1455 722 627 325 217 57
BanII G_rGCy'C
Cuts at: 1391 2564 1391
Size: 1173 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4274
BbvCI CC'TCA_GC
Cuts at: 2448 2448
Size: 5245
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3436 3470 1903
Size: 34 1499 34 3678
Fragments arranged by size:
3678 1499 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3137 4347 3137
Size: 1210 4035
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5245
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3856 136
Size: 107 122 71 631 605 2184 1525
Fragments arranged by size:
2184 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5240 5240
Size: 5245
BlpI GC'TnA_GC
Cuts at: 2328 2662 2328
Size: 334 4911
Bme1580I G_kGCm'C
Cuts at: 1790 3306 4462 1790
Size: 1516 1156 2573
Fragments arranged by size:
2573 1516 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2255 2976 3934
Size: 416 53 83 399 24 838 721 958
Cuts at: 3934 442
Size: 1753
Fragments arranged by size:
1753 958 838 721 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2228 2500 2228
Size: 272 4973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2354 2375 2477 3905 1239
Size: 1094 21 21 102 1428 2579
Fragments arranged by size:
2579 1428 1094 102 21 21
Bpu10I CC'TnA_GC
Cuts at: 2448 2448
Size: 5245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3370 4235 4533 3370
Size: 865 298 4082
Fragments arranged by size:
4082 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3908 882
Size: 617 2409 2219
Fragments arranged by size:
2409 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3036 493
Size: 711 1832 2702
Fragments arranged by size:
2702 1832 711
BsaBI GATnn'nnATC
Cuts at: 5239 5239
Size: 5245
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2349 3052 3434 4069
Size: 53 83 186 936 816 703 382 635
Cuts at: 4069 275
Size: 1451
Fragments arranged by size:
1451 936 816 703 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3678 4076 4350 4497 4908 3678
Size: 398 274 147 411 4015
Fragments arranged by size:
4015 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 1178
Size: 30 165 30 696 30 4294
Fragments arranged by size:
4294 696 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5245
BseYI C'CCAG_C
Cuts at: 2431 4448 4944 2431
Size: 2017 496 2732
Fragments arranged by size:
2732 2017 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4672
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2695 3456 4484 1540
Size: 275 186 694 761 1028 2301
Fragments arranged by size:
2301 1028 761 694 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 3306 3391 4462 2259
Size: 1047 85 1071 3042
Fragments arranged by size:
3042 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 2756 2849 2756
Size: 93 5152
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2564 3306 3391 4462 1391
Size: 399 469 305 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 469 399 305 85
BspEI T'CCGG_A
Cuts at: 4908 4908
Size: 5245
BspHI T'CATG_A
Cuts at: 3134 4864 3134
Size: 1730 3515
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2600 844
Size: 1756 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3132 1394
Size: 429 1309 3507
Fragments arranged by size:
3507 1309 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3740 3914 65
Size: 1183 2492 174 1396
Fragments arranged by size:
2492 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3889 4076 1700
Size: 110 2079 187 2869
Fragments arranged by size:
2869 2079 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5245
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5245
BssSI C'ACGA_G
Cuts at: 3305 4317 3305
Size: 1012 4233
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5245
BstBI TT'CG_AA
Cuts at: 4049 4049
Size: 5245
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5245
BstXI CCAn_nnnn'nTGG
Cuts at: 2240 2240
Size: 5245
BstYI r'GATC_y
Cuts at: 1972 2040 2575 2942 3327 3344 4796 4882 4894
Size: 68 535 367 385 17 1452 86 12
Cuts at: 4894 5240 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 535 385 367 346 86 68
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2164 513
Size: 552 939 160 3594
Fragments arranged by size:
3594 939 552 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2170 487
Size: 683 56 944 3562
Fragments arranged by size:
3562 944 683 56
BtsI GCAGTG_nn'
Cuts at: 2761 3555 3575 2761
Size: 794 20 4431
Fragments arranged by size:
4431 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5245
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5210
DraI TTT'AAA
Cuts at: 2549 2549
Size: 5245
DraIII CAC_nnn'GTG
Cuts at: 2295 2295
Size: 5245
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4252 809
Size: 507 2936 1802
Fragments arranged by size:
2936 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2394 2692
Size: 54 1425 50 275 18 177 387 298
Cuts at: 2692 3581 5210 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 387 298 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2692 1537
Size: 275 880 4090
Fragments arranged by size:
4090 880 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3175 1353
Size: 1822 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3832 4206 4352 387
Size: 1064 138 2243 374 146 1280
Fragments arranged by size:
2243 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2174 2333 2354 2375 2477 3308
Size: 393 491 51 159 21 21 102 831
Cuts at: 3308 3905 4692 4960 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 831 787 597 491 393 268 159
102 51 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2231 2231
Size: 5245
EcoO109I rG'GnC_Cy
Cuts at: 2107 2285 2452 2107
Size: 178 167 4900
Fragments arranged by size:
4900 178 167
EcoRI G'AATT_C
Cuts at: 2554 2932 2554
Size: 378 4867
FspI TGC'GCA
Cuts at: 3751 3751
Size: 5245
HaeII r_GCGC'y
Cuts at: 1678 4392 1678
Size: 2714 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 3886 3918 3960
Size: 32 135 32 527 32 1755 32 42
Cuts at: 3960 3992 1373
Size: 32 2626
Fragments arranged by size:
2626 1755 527 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2589 2835 669
Size: 988 281 651 246 3079
Fragments arranged by size:
3079 988 651 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5245
HpaI GTT'AAC
Cuts at: 2835 2835
Size: 5245
KpnI G_GTAC'C
Cuts at: 2570 2570
Size: 5245
MfeI C'AATT_G
Cuts at: 2844 2844
Size: 5245
MluI A'CGCG_T
Cuts at: 5121 5121
Size: 5245
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4359 4543 1305
Size: 3054 184 2007
Fragments arranged by size:
3054 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 10
Size: 54 1425 343 564 2859
Fragments arranged by size:
2859 1425 564 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3203 3562 3721 518
Size: 648 2037 359 159 2042
Fragments arranged by size:
2042 2037 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2038 2661 3338 4486 4731 1606
Size: 420 12 623 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 623 420 245 12
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3754
Fragments arranged by size:
3754 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5245
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5245
NotI GC'GGCC_GC
Cuts at: 2692 2692
Size: 5245
NspI r_CATG'y
Cuts at: 1132 1229 2603 4148 1132
Size: 97 1374 1545 2229
Fragments arranged by size:
2229 1545 1374 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5245
PciI A'CATG_T
Cuts at: 4144 4144
Size: 5245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2295 1108
Size: 1187 4058
PfoI T'CCnGG_A
Cuts at: 2454 2454
Size: 5245
PmeI GTTT'AAAC
Cuts at: 2549 2549
Size: 5245
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3314 3346 4827 4859 3314
Size: 32 1481 32 3700
Fragments arranged by size:
3700 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 2452 2107
Size: 345 4900
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5245
PsiI TTA'TAA
Cuts at: 2815 2815
Size: 5245
PstI C_TGCA'G
Cuts at: 830 2025 2445 2538 2597 830
Size: 1195 420 93 59 3478
Fragments arranged by size:
3478 1195 420 93 59
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5245
PvuII CAG'CTG
Cuts at: 2026 2026
Size: 5245
SalI G'TCGA_C
Cuts at: 1936 2587 1936
Size: 651 4594
SbfI CC_TGCA'GG
Cuts at: 2445 2597 2445
Size: 152 5093
ScaI AGT'ACT
Cuts at: 3493 5205 3493
Size: 1712 3533
SfcI C'TryA_G
Cuts at: 826 1045 2021 2441 2534 2593 3728 4409 4600
Size: 219 976 420 93 59 1135 681 191
Cuts at: 4600 826
Size: 1471
Fragments arranged by size:
1471 1135 976 681 420 219 191 93
59
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5245
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5245
SmaI CCC'GGG
Cuts at: 1872 2572 1872
Size: 700 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2489 3349 4250 4512 820
Size: 197 926 546 860 901 262 1553
Fragments arranged by size:
1553 926 901 860 546 262 197
SnaBI TAC'GTA
Cuts at: 493 3036 493
Size: 2543 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5245
SphI G_CATG'C
Cuts at: 1132 2603 1132
Size: 1471 3774
SspI AAT'ATT
Cuts at: 5 52 3169 5
Size: 47 3117 2081
Fragments arranged by size:
3117 2081 47
StuI AGG'CCT
Cuts at: 1517 2193 1517
Size: 676 4569
StyI C'CwwG_G
Cuts at: 513 1065 2004 2684 513
Size: 552 939 680 3074
Fragments arranged by size:
3074 939 680 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3272 3289 3442 3627 4072 325
Size: 1255 1692 17 153 185 445 1498
Fragments arranged by size:
1692 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3491 5203 96
Size: 274 80 33 51 1443 1514 1712 138
Fragments arranged by size:
1712 1514 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2355 2387 5205 5237 2355
Size: 32 2818 32 2363
Fragments arranged by size:
2818 2363 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5245
XbaI T'CTAG_A
Cuts at: 2581 2581
Size: 5245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2137 2071
Size: 66 5179
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5245
XmaI C'CCGG_G
Cuts at: 1870 2570 1870
Size: 700 4545
XmnI GAAnn'nnTTC
Cuts at: 3374 3374
Size: 5245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3053 276
Size: 53 83 186 936 1519 2468
Fragments arranged by size:
2468 1519 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI