Restriction map for
pFN21AE6560
(Circular) (Six-base) MAPSORT of: pFN21AE6560.seq Check: 6671 from: 1 to: 5200
pFN21AE6560 5200 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2344 2344
Size: 5200
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3010 278
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2543 1937
Size: 606 4594
Acc65I G'GTAC_C
Cuts at: 2199 2521 2199
Size: 322 4878
AclI AA'CG_TT
Cuts at: 1563 3327 3700 1563
Size: 1764 373 3063
Fragments arranged by size:
3063 1764 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3263 4647 4915 1632
Size: 1631 1384 268 1917
Fragments arranged by size:
1917 1631 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5003
AflIII A'CryG_T
Cuts at: 1203 4099 5076 1203
Size: 2896 977 1327
Fragments arranged by size:
2896 1327 977
AgeI A'CCGG_T
Cuts at: 2092 4031 2092
Size: 1939 3261
AhdI GACnn_n'nnGTC
Cuts at: 3929 3929
Size: 5200
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5200
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5200
AlwNI CAG_nnn'CTG
Cuts at: 2143 2943 4515 2143
Size: 800 1572 2828
Fragments arranged by size:
2828 1572 800
ApaI G_GGCC'C
Cuts at: 2365 2365
Size: 5200
ApaLI G'TGCA_C
Cuts at: 3257 4413 3257
Size: 1156 4044
ApoI r'AATT_y
Cuts at: 1493 2509 2735 2887 4996 1493
Size: 1016 226 152 2109 1697
Fragments arranged by size:
2109 1697 1016 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3754 160
Size: 872 2722 1606
Fragments arranged by size:
2722 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
AvaI C'yCGr_G
Cuts at: 1870 1943 2159 2364 2525 1870
Size: 73 216 205 161 4545
Fragments arranged by size:
4545 216 205 161 73
BamHI G'GATC_C
Cuts at: 2530 2897 2530
Size: 367 4833
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2199 2521 3976 618
Size: 325 217 627 252 160 322 1455 1842
Fragments arranged by size:
1842 1455 627 325 322 252 217 160
BanII G_rGCy'C
Cuts at: 1391 2013 2365 2519 1391
Size: 622 352 154 4072
Fragments arranged by size:
4072 622 352 154
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 5200
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4229
BbvCI CC'TCA_GC
Cuts at: 2478 2478
Size: 5200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2174 2208 3391 3425 1903
Size: 34 237 34 1183 34 3678
Fragments arranged by size:
3678 1183 237 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3092 4302 3092
Size: 1210 3990
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5200
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3811 136
Size: 107 122 71 631 605 2139 1525
Fragments arranged by size:
2139 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5195 5195
Size: 5200
BlpI GC'TnA_GC
Cuts at: 2617 2617
Size: 5200
Bme1580I G_kGCm'C
Cuts at: 1790 2365 3261 4417 1790
Size: 575 896 1156 2573
Fragments arranged by size:
2573 1156 896 575
BmgBI CAC'GTC
Cuts at: 1318 2456 1318
Size: 1138 4062
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2483 2931 3889
Size: 416 53 83 399 24 1066 448 958
Cuts at: 3889 442
Size: 1753
Fragments arranged by size:
1753 1066 958 448 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3860 1239
Size: 2621 2579
Bpu10I CC'TnA_GC
Cuts at: 2340 2478 2340
Size: 138 5062
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3325 4190 4488 3325
Size: 865 298 4037
Fragments arranged by size:
4037 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3863 882
Size: 617 2364 2219
Fragments arranged by size:
2364 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2176 2991 493
Size: 711 972 815 2702
Fragments arranged by size:
2702 972 815 711
BsaBI GATnn'nnATC
Cuts at: 5194 5194
Size: 5200
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3007 3389 4024
Size: 53 83 186 936 507 967 382 635
Cuts at: 4024 275
Size: 1451
Fragments arranged by size:
1451 967 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2092 3633 4031 4305 4452 4863 2092
Size: 1541 398 274 147 411 2429
Fragments arranged by size:
2429 1541 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4975
Fragments arranged by size:
4975 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5200
BseYI C'CCAG_C
Cuts at: 4403 4899 4403
Size: 496 4704
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2246 1163
Size: 573 510 4117
Fragments arranged by size:
4117 573 510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2093 2650 3411 4439 1540
Size: 275 186 92 557 761 1028 2301
Fragments arranged by size:
2301 1028 761 557 275 186 92
BsiHKAI G_wGCw'C
Cuts at: 3261 3346 4417 3261
Size: 85 1071 4044
Fragments arranged by size:
4044 1071 85
BsiWI C'GTAC_G
Cuts at: 2176 2176
Size: 5200
BsmI GAATG_Cn'
Cuts at: 2711 2804 2711
Size: 93 5107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2365 2519 3261 3346 4417 1391
Size: 399 223 352 154 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 352 223 154 85
BspEI T'CCGG_A
Cuts at: 4863 4863
Size: 5200
BspHI T'CATG_A
Cuts at: 3089 4819 3089
Size: 1730 3470
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2344 2555 844
Size: 1500 211 3489
Fragments arranged by size:
3489 1500 211
BsrBI CCG'CTC
Cuts at: 1394 1823 3087 1394
Size: 429 1264 3507
Fragments arranged by size:
3507 1264 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3695 3869 65
Size: 1183 2447 174 1396
Fragments arranged by size:
2447 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2092 3844 4031 1700
Size: 110 282 1752 187 2869
Fragments arranged by size:
2869 1752 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5200
BssSI C'ACGA_G
Cuts at: 2014 3260 4272 2014
Size: 1246 1012 2942
Fragments arranged by size:
2942 1246 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5200
BstBI TT'CG_AA
Cuts at: 4004 4004
Size: 5200
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5200
BstYI r'GATC_y
Cuts at: 1972 2409 2530 2897 3282 3299 4751 4837 4849
Size: 437 121 367 385 17 1452 86 12
Cuts at: 4849 5195 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 437 385 367 346 121 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2288 2440 2288
Size: 152 5048
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 513
Size: 552 939 57 3652
Fragments arranged by size:
3652 939 552 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4461
Fragments arranged by size:
4461 683 56
BtsI GCAGTG_nn'
Cuts at: 2716 3510 3530 2716
Size: 794 20 4386
Fragments arranged by size:
4386 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5200
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5165
DraI TTT'AAA
Cuts at: 2504 2504
Size: 5200
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4207 809
Size: 507 2891 1802
Fragments arranged by size:
2891 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2647 3536 5165
Size: 54 1425 50 275 18 817 889 1629
Cuts at: 5165 8
Size: 43
Fragments arranged by size:
1629 1425 889 817 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2647 1537
Size: 275 835 4090
Fragments arranged by size:
4090 835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3130 1353
Size: 1777 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2038 3787 4161 4307 387
Size: 1064 138 449 1749 374 146 1280
Fragments arranged by size:
1749 1280 1064 449 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3263 3860 4647 4915 1239
Size: 393 1631 597 787 268 1524
Fragments arranged by size:
1631 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2477 2477
Size: 5200
EcoO109I rG'GnC_Cy
Cuts at: 2314 2361 2314
Size: 47 5153
EcoRI G'AATT_C
Cuts at: 2509 2887 2509
Size: 378 4822
FspI TGC'GCA
Cuts at: 3706 3706
Size: 5200
HaeII r_GCGC'y
Cuts at: 1678 2043 4347 1678
Size: 365 2304 2531
Fragments arranged by size:
2531 2304 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 3841 3873 3915
Size: 32 135 32 878 32 1359 32 42
Cuts at: 3915 3947 1373
Size: 32 2626
Fragments arranged by size:
2626 1359 878 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2544 2790 669
Size: 988 281 606 246 3079
Fragments arranged by size:
3079 988 606 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5200
HpaI GTT'AAC
Cuts at: 2790 2790
Size: 5200
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 5200
KpnI G_GTAC'C
Cuts at: 2203 2525 2203
Size: 322 4878
MfeI C'AATT_G
Cuts at: 2799 2799
Size: 5200
MluI A'CGCG_T
Cuts at: 5076 5076
Size: 5200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2113 4314 4498 1305
Size: 808 2201 184 2007
Fragments arranged by size:
2201 2007 808 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3378
Fragments arranged by size:
3378 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3158 3517 3676 518
Size: 648 1992 359 159 2042
Fragments arranged by size:
2042 1992 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2298 2616 3293 4441 4686 1606
Size: 423 269 318 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 423 318 269 245
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 5200
NcoI C'CATG_G
Cuts at: 513 1065 2004 2061 513
Size: 552 939 57 3652
Fragments arranged by size:
3652 939 552 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5200
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5200
NotI GC'GGCC_GC
Cuts at: 2647 2647
Size: 5200
NspI r_CATG'y
Cuts at: 1132 1229 2558 4103 1132
Size: 97 1329 1545 2229
Fragments arranged by size:
2229 1545 1329 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5200
PciI A'CATG_T
Cuts at: 4099 4099
Size: 5200
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5200
PfoI T'CCnGG_A
Cuts at: 2097 2381 2097
Size: 284 4916
PmeI GTTT'AAAC
Cuts at: 2504 2504
Size: 5200
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 4782 4814 3269
Size: 32 1481 32 3655
Fragments arranged by size:
3655 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2314 2314
Size: 5200
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5200
PsiI TTA'TAA
Cuts at: 2770 2770
Size: 5200
PspOMI G'GGCC_C
Cuts at: 2361 2361
Size: 5200
PstI C_TGCA'G
Cuts at: 830 2169 2552 830
Size: 1339 383 3478
Fragments arranged by size:
3478 1339 383
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5200
SalI G'TCGA_C
Cuts at: 1936 2542 1936
Size: 606 4594
SbfI CC_TGCA'GG
Cuts at: 2552 2552
Size: 5200
ScaI AGT'ACT
Cuts at: 3448 5160 3448
Size: 1712 3488
SfcI C'TryA_G
Cuts at: 826 1045 2165 2548 3683 4364 4555 826
Size: 219 1120 383 1135 681 191 1471
Fragments arranged by size:
1471 1135 1120 681 383 219 191
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 5200
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5200
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5200
SmaI CCC'GGG
Cuts at: 1872 2527 1872
Size: 655 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 3304 4205 4467 820
Size: 197 926 216 1145 901 262 1553
Fragments arranged by size:
1553 1145 926 901 262 216 197
SnaBI TAC'GTA
Cuts at: 493 2991 493
Size: 2498 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5200
SphI G_CATG'C
Cuts at: 1132 2558 1132
Size: 1426 3774
SspI AAT'ATT
Cuts at: 5 52 3124 5
Size: 47 3072 2081
Fragments arranged by size:
3072 2081 47
StuI AGG'CCT
Cuts at: 1517 2472 1517
Size: 955 4245
StyI C'CwwG_G
Cuts at: 513 1065 2004 2061 2639 513
Size: 552 939 57 578 3074
Fragments arranged by size:
3074 939 578 552 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3227 3244 3397 3582 4027 325
Size: 1255 1647 17 153 185 445 1498
Fragments arranged by size:
1647 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3446 5158 96
Size: 274 80 33 51 1443 1469 1712 138
Fragments arranged by size:
1712 1469 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5160 5192 5160
Size: 32 5168
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5200
XbaI T'CTAG_A
Cuts at: 2536 2536
Size: 5200
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 5200
XhoI C'TCGA_G
Cuts at: 1943 2159 1943
Size: 216 4984
XmaI C'CCGG_G
Cuts at: 1870 2525 1870
Size: 655 4545
XmnI GAAnn'nnTTC
Cuts at: 3329 3329
Size: 5200
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3008 276
Size: 53 83 186 936 1474 2468
Fragments arranged by size:
2468 1474 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsmBI
BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI