Restriction map for
pFN21AE6558
(Circular) (Six-base) MAPSORT of: pFN21AE6558.seq Check: 8706 from: 1 to: 5173
pFN21AE6558 5173 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2983 278
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2516 1937
Size: 579 4594
Acc65I G'GTAC_C
Cuts at: 2494 2494
Size: 5173
AclI AA'CG_TT
Cuts at: 1563 3300 3673 1563
Size: 1737 373 3063
Fragments arranged by size:
3063 1737 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3236 4620 4888 1632
Size: 1604 1384 268 1917
Fragments arranged by size:
1917 1604 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4976
AflIII A'CryG_T
Cuts at: 1203 4072 5049 1203
Size: 2869 977 1327
Fragments arranged by size:
2869 1327 977
AgeI A'CCGG_T
Cuts at: 4004 4004
Size: 5173
AhdI GACnn_n'nnGTC
Cuts at: 3902 3902
Size: 5173
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5173
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5173
AlwNI CAG_nnn'CTG
Cuts at: 2450 2916 4488 2450
Size: 466 1572 3135
Fragments arranged by size:
3135 1572 466
ApaLI G'TGCA_C
Cuts at: 2354 3230 4386 2354
Size: 876 1156 3141
Fragments arranged by size:
3141 1156 876
ApoI r'AATT_y
Cuts at: 1493 2482 2708 2860 4969 1493
Size: 989 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3727 160
Size: 872 2695 1606
Fragments arranged by size:
2695 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
AvaI C'yCGr_G
Cuts at: 1870 1943 2049 2498 1870
Size: 73 106 449 4545
Fragments arranged by size:
4545 449 106 73
BamHI G'GATC_C
Cuts at: 2503 2870 2503
Size: 367 4806
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2494 3949 618
Size: 325 217 627 707 1455 1842
Fragments arranged by size:
1842 1455 707 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2492 1391
Size: 744 357 4072
Fragments arranged by size:
4072 744 357
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2286 928
Size: 971 387 3815
Fragments arranged by size:
3815 971 387
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2363 2397 3364 3398 1903
Size: 34 50 34 342 34 967 34 3678
Fragments arranged by size:
3678 967 342 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3065 4275 3065
Size: 1210 3963
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5173
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3784 136
Size: 107 122 71 631 605 337 1775 1525
Fragments arranged by size:
1775 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5168 5168
Size: 5173
BlpI GC'TnA_GC
Cuts at: 2590 2590
Size: 5173
Bme1580I G_kGCm'C
Cuts at: 1790 2358 3234 4390 1790
Size: 568 876 1156 2573
Fragments arranged by size:
2573 1156 876 568
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5173
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2904 3862 442
Size: 416 53 83 399 24 1487 958 1753
Fragments arranged by size:
1753 1487 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3833 1239
Size: 2594 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4163 4461 3298
Size: 865 298 4010
Fragments arranged by size:
4010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 3836 882
Size: 617 644 1693 2219
Fragments arranged by size:
2219 1693 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 2964 493
Size: 711 1760 2702
Fragments arranged by size:
2702 1760 711
BsaBI GATnn'nnATC
Cuts at: 5167 5167
Size: 5173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2980 3362 3997 275
Size: 53 83 186 936 1447 382 635 1451
Fragments arranged by size:
1451 1447 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3606 4004 4278 4425 4836 3606
Size: 398 274 147 411 3943
Fragments arranged by size:
3943 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4948
Fragments arranged by size:
4948 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5173
BseYI C'CCAG_C
Cuts at: 2088 2326 4376 4872 2088
Size: 238 2050 496 2389
Fragments arranged by size:
2389 2050 496 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4600
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2623 3384 4412 1540
Size: 275 186 622 761 1028 2301
Fragments arranged by size:
2301 1028 761 622 275 186
BsiHKAI G_wGCw'C
Cuts at: 2358 3234 3319 4390 2358
Size: 876 85 1071 3141
Fragments arranged by size:
3141 1071 876 85
BsmI GAATG_Cn'
Cuts at: 2684 2777 2684
Size: 93 5080
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2358 2492 3234 3319 4390 1391
Size: 399 345 223 134 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 345 223 134 85
BspEI T'CCGG_A
Cuts at: 4836 4836
Size: 5173
BspHI T'CATG_A
Cuts at: 3062 4792 3062
Size: 1730 3443
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2424 2528 844
Size: 1580 104 3489
Fragments arranged by size:
3489 1580 104
BsrBI CCG'CTC
Cuts at: 1394 1823 3060 1394
Size: 429 1237 3507
Fragments arranged by size:
3507 1237 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2384 3668 3842 65
Size: 1183 1136 1284 174 1396
Fragments arranged by size:
1396 1284 1183 1136 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2098 3817 4004 1700
Size: 110 198 90 1719 187 2869
Fragments arranged by size:
2869 1719 198 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5173
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5173
BssSI C'ACGA_G
Cuts at: 3233 4245 3233
Size: 1012 4161
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2425 794
Size: 1631 3542
BstBI TT'CG_AA
Cuts at: 3977 3977
Size: 5173
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5173
BstXI CCAn_nnnn'nTGG
Cuts at: 2197 2197
Size: 5173
BstYI r'GATC_y
Cuts at: 1972 2503 2870 3255 3272 4724 4810 4822 5168
Size: 531 367 385 17 1452 86 12 346
Cuts at: 5168 1972
Size: 1977
Fragments arranged by size:
1977 1452 531 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2064 2196 513
Size: 552 999 132 3490
Fragments arranged by size:
3490 999 552 132
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4434
Fragments arranged by size:
4434 683 56
BtsI GCAGTG_nn'
Cuts at: 2426 2689 3483 3503 2426
Size: 263 794 20 4096
Fragments arranged by size:
4096 794 263 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5173
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5138
DraI TTT'AAA
Cuts at: 2477 2477
Size: 5173
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4180 809
Size: 507 2864 1802
Fragments arranged by size:
2864 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2620 3509
Size: 54 1425 50 275 18 540 250 889
Cuts at: 3509 5138 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 540 275 250 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2620 1537
Size: 275 808 4090
Fragments arranged by size:
4090 808 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3103 1353
Size: 1750 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3760 4134 4280 387
Size: 1064 138 2171 374 146 1280
Fragments arranged by size:
2171 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3236 3833 4620 4888 1239
Size: 393 1604 597 787 268 1524
Fragments arranged by size:
1604 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2482 2860 2482
Size: 378 4795
FspI TGC'GCA
Cuts at: 3679 3679
Size: 5173
HaeII r_GCGC'y
Cuts at: 1678 2015 2212 4320 1678
Size: 337 197 2108 2531
Fragments arranged by size:
2531 2108 337 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2099 2131 3814 3846 3888
Size: 32 135 32 527 32 1683 32 42
Cuts at: 3888 3920 1373
Size: 32 2626
Fragments arranged by size:
2626 1683 527 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2227 2517 2763 669
Size: 988 281 289 290 246 3079
Fragments arranged by size:
3079 988 290 289 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5173
HpaI GTT'AAC
Cuts at: 2763 2763
Size: 5173
KpnI G_GTAC'C
Cuts at: 2498 2498
Size: 5173
MfeI C'AATT_G
Cuts at: 2772 2772
Size: 5173
MluI A'CGCG_T
Cuts at: 5049 5049
Size: 5173
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2108 4287 4471 1305
Size: 803 2179 184 2007
Fragments arranged by size:
2179 2007 803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3351
Fragments arranged by size:
3351 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3131 3490 3649 518
Size: 648 1965 359 159 2042
Fragments arranged by size:
2042 1965 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2589 3266 4414 4659 1606
Size: 983 677 1148 245 2120
Fragments arranged by size:
2120 1148 983 677 245
NaeI GCC'GGC
Cuts at: 2010 2100 2010
Size: 90 5083
NcoI C'CATG_G
Cuts at: 513 1065 2064 513
Size: 552 999 3622
Fragments arranged by size:
3622 999 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5173
NgoMIV G'CCGG_C
Cuts at: 2008 2098 2008
Size: 90 5083
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5173
NotI GC'GGCC_GC
Cuts at: 2620 2620
Size: 5173
NspI r_CATG'y
Cuts at: 1132 1229 2531 4076 1132
Size: 97 1302 1545 2229
Fragments arranged by size:
2229 1545 1302 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5173
PciI A'CATG_T
Cuts at: 4072 4072
Size: 5173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5173
PmeI GTTT'AAAC
Cuts at: 2477 2477
Size: 5173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 4755 4787 3242
Size: 32 1481 32 3628
Fragments arranged by size:
3628 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5173
PsiI TTA'TAA
Cuts at: 2743 2743
Size: 5173
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2396 2428 2396
Size: 32 5141
PstI C_TGCA'G
Cuts at: 830 2146 2421 2525 830
Size: 1316 275 104 3478
Fragments arranged by size:
3478 1316 275 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5173
SalI G'TCGA_C
Cuts at: 1936 2515 1936
Size: 579 4594
SbfI CC_TGCA'GG
Cuts at: 2525 2525
Size: 5173
ScaI AGT'ACT
Cuts at: 3421 5133 3421
Size: 1712 3461
SfcI C'TryA_G
Cuts at: 826 1045 2142 2417 2521 3656 4337 4528 826
Size: 219 1097 275 104 1135 681 191 1471
Fragments arranged by size:
1471 1135 1097 681 275 219 191 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5173
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5173
SmaI CCC'GGG
Cuts at: 1872 2500 1872
Size: 628 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3277 4178 4440 820
Size: 197 926 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2964 493
Size: 2471 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5173
SphI G_CATG'C
Cuts at: 1132 2531 1132
Size: 1399 3774
SspI AAT'ATT
Cuts at: 5 52 3097 5
Size: 47 3045 2081
Fragments arranged by size:
3045 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5173
StyI C'CwwG_G
Cuts at: 513 1065 2064 2178 2319 2612 513
Size: 552 999 114 141 293 3074
Fragments arranged by size:
3074 999 552 293 141 114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 2413 3200 3217 3370 3555 4000
Size: 1255 545 288 787 17 153 185 445
Cuts at: 4000 325
Size: 1498
Fragments arranged by size:
1498 1255 787 545 445 288 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3419 5131 96
Size: 274 80 33 51 1443 1442 1712 138
Fragments arranged by size:
1712 1443 1442 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5133 5165 5133
Size: 32 5141
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5173
XbaI T'CTAG_A
Cuts at: 2509 2509
Size: 5173
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2197 2197
Size: 5173
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5173
XmaI C'CCGG_G
Cuts at: 1870 2498 1870
Size: 628 4545
XmnI GAAnn'nnTTC
Cuts at: 3302 3302
Size: 5173
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2981 276
Size: 53 83 186 936 1447 2468
Fragments arranged by size:
2468 1447 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI