Restriction map for
pFN21AE6557
(Circular) (Six-base) MAPSORT of: pFN21AE6557.seq Check: 4865 from: 1 to: 5164
pFN21AE6557 5164 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2974 278
Size: 53 83 186 936 1438 2468
Fragments arranged by size:
2468 1438 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2507 1937
Size: 570 4594
Acc65I G'GTAC_C
Cuts at: 2485 2485
Size: 5164
AclI AA'CG_TT
Cuts at: 1563 3291 3664 1563
Size: 1728 373 3063
Fragments arranged by size:
3063 1728 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2481 3227 4611 4879 1632
Size: 849 746 1384 268 1917
Fragments arranged by size:
1917 1384 849 746 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4967
AflIII A'CryG_T
Cuts at: 1203 4063 5040 1203
Size: 2860 977 1327
Fragments arranged by size:
2860 1327 977
AgeI A'CCGG_T
Cuts at: 3995 3995
Size: 5164
AhdI GACnn_n'nnGTC
Cuts at: 3893 3893
Size: 5164
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5164
AlwNI CAG_nnn'CTG
Cuts at: 2907 4479 2907
Size: 1572 3592
ApaLI G'TGCA_C
Cuts at: 3221 4377 3221
Size: 1156 4008
ApoI r'AATT_y
Cuts at: 1493 2473 2699 2851 4960 1493
Size: 980 226 152 2109 1697
Fragments arranged by size:
2109 1697 980 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3718 160
Size: 872 2686 1606
Fragments arranged by size:
2686 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5164
AvaI C'yCGr_G
Cuts at: 1870 1943 2489 1870
Size: 73 546 4545
Fragments arranged by size:
4545 546 73
BamHI G'GATC_C
Cuts at: 2494 2861 2494
Size: 367 4797
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2485 3940 618
Size: 325 217 627 698 1455 1842
Fragments arranged by size:
1842 1455 698 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2483 1391
Size: 1092 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 928
Size: 971 412 3781
Fragments arranged by size:
3781 971 412
BbvCI CC'TCA_GC
Cuts at: 2271 2271
Size: 5164
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3355 3389 1903
Size: 34 50 34 1334 34 3678
Fragments arranged by size:
3678 1334 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3056 4266 3056
Size: 1210 3954
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2354 3775 136
Size: 107 122 71 631 605 682 1421 1525
Fragments arranged by size:
1525 1421 682 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5159 5159
Size: 5164
BlpI GC'TnA_GC
Cuts at: 2581 2581
Size: 5164
Bme1580I G_kGCm'C
Cuts at: 1790 3225 4381 1790
Size: 1435 1156 2573
Fragments arranged by size:
2573 1435 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5164
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2895 3853 442
Size: 416 53 83 399 24 1478 958 1753
Fragments arranged by size:
1753 1478 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2021 3824 1239
Size: 782 1803 2579
Fragments arranged by size:
2579 1803 782
Bpu10I CC'TnA_GC
Cuts at: 2271 2271
Size: 5164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3289 4154 4452 3289
Size: 865 298 4001
Fragments arranged by size:
4001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2263 3827 882
Size: 617 764 1564 2219
Fragments arranged by size:
2219 1564 764 617
BsaAI yAC'GTr
Cuts at: 493 1204 2955 493
Size: 711 1751 2702
Fragments arranged by size:
2702 1751 711
BsaBI GATnn'nnATC
Cuts at: 5158 5158
Size: 5164
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 3353 3988 275
Size: 53 83 186 936 1438 382 635 1451
Fragments arranged by size:
1451 1438 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3597 3995 4269 4416 4827 3597
Size: 398 274 147 411 3934
Fragments arranged by size:
3934 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4939
Fragments arranged by size:
4939 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5164
BseYI C'CCAG_C
Cuts at: 4367 4863 4367
Size: 496 4668
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4591
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2614 3375 4403 1540
Size: 275 186 613 761 1028 2301
Fragments arranged by size:
2301 1028 761 613 275 186
BsiHKAI G_wGCw'C
Cuts at: 3225 3310 4381 3225
Size: 85 1071 4008
Fragments arranged by size:
4008 1071 85
BsmI GAATG_Cn'
Cuts at: 2097 2675 2768 2097
Size: 578 93 4493
Fragments arranged by size:
4493 578 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2156 2156
Size: 5164
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2483 3225 3310 4381 1391
Size: 399 693 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 693 399 85
BspEI T'CCGG_A
Cuts at: 4827 4827
Size: 5164
BspHI T'CATG_A
Cuts at: 3053 4783 3053
Size: 1730 3434
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2405 2519 844
Size: 1561 114 3489
Fragments arranged by size:
3489 1561 114
BsrBI CCG'CTC
Cuts at: 1394 1823 3051 1394
Size: 429 1228 3507
Fragments arranged by size:
3507 1228 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3659 3833 65
Size: 1183 2411 174 1396
Fragments arranged by size:
2411 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3808 3995 1700
Size: 110 1998 187 2869
Fragments arranged by size:
2869 1998 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5164
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5164
BssSI C'ACGA_G
Cuts at: 3224 4236 3224
Size: 1012 4152
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5164
BstBI TT'CG_AA
Cuts at: 3968 3968
Size: 5164
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5164
BstYI r'GATC_y
Cuts at: 1972 2048 2494 2861 3246 3263 4715 4801 4813
Size: 76 446 367 385 17 1452 86 12
Cuts at: 4813 5159 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 446 385 367 346 86 76
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4612
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4425
Fragments arranged by size:
4425 683 56
BtsI GCAGTG_nn'
Cuts at: 2680 3474 3494 2680
Size: 794 20 4350
Fragments arranged by size:
4350 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5164
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5129
DraI TTT'AAA
Cuts at: 2468 2468
Size: 5164
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4171 809
Size: 507 2855 1802
Fragments arranged by size:
2855 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2611 3500 5129
Size: 54 1425 50 275 18 781 889 1629
Cuts at: 5129 8
Size: 43
Fragments arranged by size:
1629 1425 889 781 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2611 1537
Size: 275 799 4090
Fragments arranged by size:
4090 799 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2152 2183 3094 1353
Size: 799 31 911 3423
Fragments arranged by size:
3423 911 799 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3751 4125 4271 387
Size: 1064 138 2162 374 146 1280
Fragments arranged by size:
2162 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2481 3227 3824 4611 4879 1239
Size: 393 389 460 746 597 787 268 1524
Fragments arranged by size:
1524 787 746 597 460 393 389 268
EcoRI G'AATT_C
Cuts at: 2473 2851 2473
Size: 378 4786
FspI TGC'GCA
Cuts at: 3670 3670
Size: 5164
HaeII r_GCGC'y
Cuts at: 1678 4311 1678
Size: 2633 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3805 3837 3879 3911 1373
Size: 32 135 32 2233 32 42 32 2626
Fragments arranged by size:
2626 2233 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2508 2754 669
Size: 988 281 570 246 3079
Fragments arranged by size:
3079 988 570 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5164
HpaI GTT'AAC
Cuts at: 2754 2754
Size: 5164
KpnI G_GTAC'C
Cuts at: 2489 2489
Size: 5164
MfeI C'AATT_G
Cuts at: 2763 2763
Size: 5164
MluI A'CGCG_T
Cuts at: 5040 5040
Size: 5164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2290 4278 4462 1305
Size: 985 1988 184 2007
Fragments arranged by size:
2007 1988 985 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3342
Fragments arranged by size:
3342 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3122 3481 3640 518
Size: 648 1956 359 159 2042
Fragments arranged by size:
2042 1956 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2580 3257 4405 4650 1606
Size: 974 677 1148 245 2120
Fragments arranged by size:
2120 1148 974 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4612
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5164
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5164
NotI GC'GGCC_GC
Cuts at: 2611 2611
Size: 5164
NspI r_CATG'y
Cuts at: 1132 1229 2522 4067 1132
Size: 97 1293 1545 2229
Fragments arranged by size:
2229 1545 1293 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5164
PciI A'CATG_T
Cuts at: 4063 4063
Size: 5164
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5164
PmeI GTTT'AAAC
Cuts at: 2468 2468
Size: 5164
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3233 3265 4746 4778 3233
Size: 32 1481 32 3619
Fragments arranged by size:
3619 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5164
PsiI TTA'TAA
Cuts at: 2734 2734
Size: 5164
PstI C_TGCA'G
Cuts at: 830 2516 830
Size: 1686 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5164
SalI G'TCGA_C
Cuts at: 1936 2506 1936
Size: 570 4594
SbfI CC_TGCA'GG
Cuts at: 2516 2516
Size: 5164
ScaI AGT'ACT
Cuts at: 3412 5124 3412
Size: 1712 3452
SfcI C'TryA_G
Cuts at: 826 1045 2512 3647 4328 4519 826
Size: 219 1467 1135 681 191 1471
Fragments arranged by size:
1471 1467 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5164
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5164
SmaI CCC'GGG
Cuts at: 1872 2491 1872
Size: 619 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3268 4169 4431 820
Size: 197 926 1325 901 262 1553
Fragments arranged by size:
1553 1325 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2955 493
Size: 2462 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5164
SphI G_CATG'C
Cuts at: 1132 2522 1132
Size: 1390 3774
SspI AAT'ATT
Cuts at: 5 52 3088 5
Size: 47 3036 2081
Fragments arranged by size:
3036 2081 47
StuI AGG'CCT
Cuts at: 1517 2357 1517
Size: 840 4324
StyI C'CwwG_G
Cuts at: 513 1065 2252 2603 513
Size: 552 1187 351 3074
Fragments arranged by size:
3074 1187 552 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 3191 3208 3361 3546 3991 325
Size: 1255 432 1179 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1179 445 432 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3410 5122 96
Size: 274 80 33 51 1443 1433 1712 138
Fragments arranged by size:
1712 1443 1433 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5124 5156 5124
Size: 32 5132
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5164
XbaI T'CTAG_A
Cuts at: 2380 2500 2380
Size: 120 5044
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2059 2059
Size: 5164
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5164
XmaI C'CCGG_G
Cuts at: 1870 2489 1870
Size: 619 4545
XmnI GAAnn'nnTTC
Cuts at: 3293 3293
Size: 5164
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2972 276
Size: 53 83 186 936 1438 2468
Fragments arranged by size:
2468 1438 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI