Restriction map for
pFN21AE6535
(Circular) (Six-base) MAPSORT of: pFN21AE6535.seq Check: 497 from: 1 to: 4915
pFN21AE6535 4915 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2725 278
Size: 53 83 186 936 1189 2468
Fragments arranged by size:
2468 1189 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2258 1937
Size: 321 4594
Acc65I G'GTAC_C
Cuts at: 2236 2236
Size: 4915
AclI AA'CG_TT
Cuts at: 1563 3042 3415 1563
Size: 1479 373 3063
Fragments arranged by size:
3063 1479 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2978 4362 4630 1632
Size: 1346 1384 268 1917
Fragments arranged by size:
1917 1384 1346 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4718
AflIII A'CryG_T
Cuts at: 1203 3814 4791 1203
Size: 2611 977 1327
Fragments arranged by size:
2611 1327 977
AgeI A'CCGG_T
Cuts at: 3746 3746
Size: 4915
AhdI GACnn_n'nnGTC
Cuts at: 2033 3644 2033
Size: 1611 3304
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4915
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4915
AlwNI CAG_nnn'CTG
Cuts at: 2658 4230 2658
Size: 1572 3343
ApaLI G'TGCA_C
Cuts at: 2972 4128 2972
Size: 1156 3759
ApoI r'AATT_y
Cuts at: 1493 2224 2450 2602 4711 1493
Size: 731 226 152 2109 1697
Fragments arranged by size:
2109 1697 731 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3469 160
Size: 872 2437 1606
Fragments arranged by size:
2437 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4915
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 1870
Size: 73 297 4545
Fragments arranged by size:
4545 297 73
BamHI G'GATC_C
Cuts at: 2245 2612 2245
Size: 367 4548
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 3691 618
Size: 325 217 627 449 1455 1842
Fragments arranged by size:
1842 1455 627 449 325 217
BanII G_rGCy'C
Cuts at: 1391 2234 1391
Size: 843 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3944
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3106 3140 1903
Size: 34 1169 34 3678
Fragments arranged by size:
3678 1169 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2807 4017 2807
Size: 1210 3705
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4915
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3526 136
Size: 107 122 71 631 605 1854 1525
Fragments arranged by size:
1854 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4910 4910
Size: 4915
BlpI GC'TnA_GC
Cuts at: 2332 2332
Size: 4915
Bme1580I G_kGCm'C
Cuts at: 1790 2976 4132 1790
Size: 1186 1156 2573
Fragments arranged by size:
2573 1186 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4915
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2646 3604 442
Size: 416 53 83 399 24 1229 958 1753
Fragments arranged by size:
1753 1229 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2158 2190 3575 1239
Size: 919 32 1385 2579
Fragments arranged by size:
2579 1385 919 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3040 3905 4203 3040
Size: 865 298 3752
Fragments arranged by size:
3752 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3578 882
Size: 617 2079 2219
Fragments arranged by size:
2219 2079 617
BsaAI yAC'GTr
Cuts at: 493 1204 2706 493
Size: 711 1502 2702
Fragments arranged by size:
2702 1502 711
BsaBI GATnn'nnATC
Cuts at: 4909 4909
Size: 4915
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2722 3104 3739 275
Size: 53 83 186 936 1189 382 635 1451
Fragments arranged by size:
1451 1189 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3348 3746 4020 4167 4578 3348
Size: 398 274 147 411 3685
Fragments arranged by size:
3685 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4690
Fragments arranged by size:
4690 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4915
BseYI C'CCAG_C
Cuts at: 4118 4614 4118
Size: 496 4419
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2365 3126 4154 1540
Size: 275 186 364 761 1028 2301
Fragments arranged by size:
2301 1028 761 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 2976 3061 4132 2976
Size: 85 1071 3759
Fragments arranged by size:
3759 1071 85
BsmI GAATG_Cn'
Cuts at: 2426 2519 2426
Size: 93 4822
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2234 2976 3061 4132 1391
Size: 399 444 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 444 399 85
BspEI T'CCGG_A
Cuts at: 4578 4578
Size: 4915
BspHI T'CATG_A
Cuts at: 2804 4534 2804
Size: 1730 3185
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2270 844
Size: 1426 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2802 1394
Size: 429 979 3507
Fragments arranged by size:
3507 979 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3410 3584 65
Size: 1183 2162 174 1396
Fragments arranged by size:
2162 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3559 3746 1700
Size: 110 1749 187 2869
Fragments arranged by size:
2869 1749 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4915
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4915
BssSI C'ACGA_G
Cuts at: 2975 3987 2975
Size: 1012 3903
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4915
BstBI TT'CG_AA
Cuts at: 3719 3719
Size: 4915
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4915
BstYI r'GATC_y
Cuts at: 1972 2245 2612 2997 3014 4466 4552 4564 4910
Size: 273 367 385 17 1452 86 12 346
Cuts at: 4910 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 273 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4176
Fragments arranged by size:
4176 683 56
BtsI GCAGTG_nn'
Cuts at: 2431 3225 3245 2431
Size: 794 20 4101
Fragments arranged by size:
4101 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4915
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4880
DraI TTT'AAA
Cuts at: 2219 2219
Size: 4915
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3922 809
Size: 507 2606 1802
Fragments arranged by size:
2606 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2362 3251 4880
Size: 54 1425 50 275 18 532 889 1629
Cuts at: 4880 8
Size: 43
Fragments arranged by size:
1629 1425 889 532 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2362 1537
Size: 275 550 4090
Fragments arranged by size:
4090 550 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2845 1353
Size: 1492 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3502 3876 4022 387
Size: 1064 138 1913 374 146 1280
Fragments arranged by size:
1913 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2158 2190 2978 3575 4362 4630 1239
Size: 393 526 32 788 597 787 268 1524
Fragments arranged by size:
1524 788 787 597 526 393 268 32
EcoRI G'AATT_C
Cuts at: 2224 2602 2224
Size: 378 4537
FspI TGC'GCA
Cuts at: 3421 3421
Size: 4915
HaeII r_GCGC'y
Cuts at: 1678 4062 1678
Size: 2384 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2019 2051 3556 3588 3630
Size: 32 135 32 447 32 1505 32 42
Cuts at: 3630 3662 1373
Size: 32 2626
Fragments arranged by size:
2626 1505 447 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2259 2505 669
Size: 988 281 321 246 3079
Fragments arranged by size:
3079 988 321 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4915
HpaI GTT'AAC
Cuts at: 2505 2505
Size: 4915
KpnI G_GTAC'C
Cuts at: 2240 2240
Size: 4915
MfeI C'AATT_G
Cuts at: 2514 2514
Size: 4915
MluI A'CGCG_T
Cuts at: 4791 4791
Size: 4915
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4029 4213 1305
Size: 2724 184 2007
Fragments arranged by size:
2724 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3093
Fragments arranged by size:
3093 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 2873 3232 3391 518
Size: 648 876 831 359 159 2042
Fragments arranged by size:
2042 876 831 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2331 3008 4156 4401 1606
Size: 725 677 1148 245 2120
Fragments arranged by size:
2120 1148 725 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4363
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4915
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4915
NotI GC'GGCC_GC
Cuts at: 2362 2362
Size: 4915
NspI r_CATG'y
Cuts at: 1132 1229 2273 3818 1132
Size: 97 1044 1545 2229
Fragments arranged by size:
2229 1545 1044 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4915
PciI A'CATG_T
Cuts at: 3814 3814
Size: 4915
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4915
PfoI T'CCnGG_A
Cuts at: 2059 2059
Size: 4915
PmeI GTTT'AAAC
Cuts at: 2219 2219
Size: 4915
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 4497 4529 2984
Size: 32 1481 32 3370
Fragments arranged by size:
3370 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4915
PsiI TTA'TAA
Cuts at: 2485 2485
Size: 4915
PstI C_TGCA'G
Cuts at: 830 2267 830
Size: 1437 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4915
SalI G'TCGA_C
Cuts at: 1936 2257 1936
Size: 321 4594
SbfI CC_TGCA'GG
Cuts at: 2267 2267
Size: 4915
ScaI AGT'ACT
Cuts at: 3163 4875 3163
Size: 1712 3203
SfcI C'TryA_G
Cuts at: 826 1045 2263 3398 4079 4270 826
Size: 219 1218 1135 681 191 1471
Fragments arranged by size:
1471 1218 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4915
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4915
SmaI CCC'GGG
Cuts at: 1872 2242 1872
Size: 370 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3019 3920 4182 820
Size: 197 926 1076 901 262 1553
Fragments arranged by size:
1553 1076 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2706 493
Size: 2213 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4915
SphI G_CATG'C
Cuts at: 1132 2273 1132
Size: 1141 3774
SspI AAT'ATT
Cuts at: 5 52 2839 5
Size: 47 2787 2081
Fragments arranged by size:
2787 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4915
StyI C'CwwG_G
Cuts at: 513 1065 2354 513
Size: 552 1289 3074
Fragments arranged by size:
3074 1289 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2942 2959 3112 3297 3742 325
Size: 1255 1362 17 153 185 445 1498
Fragments arranged by size:
1498 1362 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3161 4873 96
Size: 274 80 33 51 1443 1184 1712 138
Fragments arranged by size:
1712 1443 1184 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4875 4907 4875
Size: 32 4883
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4915
XbaI T'CTAG_A
Cuts at: 2251 2251
Size: 4915
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4915
XmaI C'CCGG_G
Cuts at: 1870 2240 1870
Size: 370 4545
XmnI GAAnn'nnTTC
Cuts at: 3044 3044
Size: 4915
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2723 276
Size: 53 83 186 936 1189 2468
Fragments arranged by size:
2468 1189 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI