Restriction map for
pFN21AE6534
(Circular) (Six-base) MAPSORT of: pFN21AE6534.seq Check: 1062 from: 1 to: 4897
pFN21AE6534 4897 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2707 278
Size: 53 83 186 936 1171 2468
Fragments arranged by size:
2468 1171 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2240 1937
Size: 303 4594
Acc65I G'GTAC_C
Cuts at: 2218 2218
Size: 4897
AclI AA'CG_TT
Cuts at: 1563 3024 3397 1563
Size: 1461 373 3063
Fragments arranged by size:
3063 1461 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2075 2960 4344 4612 1632
Size: 443 885 1384 268 1917
Fragments arranged by size:
1917 1384 885 443 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4700
AflIII A'CryG_T
Cuts at: 1203 3796 4773 1203
Size: 2593 977 1327
Fragments arranged by size:
2593 1327 977
AgeI A'CCGG_T
Cuts at: 3728 3728
Size: 4897
AhdI GACnn_n'nnGTC
Cuts at: 3626 3626
Size: 4897
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4897
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4897
AlwNI CAG_nnn'CTG
Cuts at: 2640 4212 2640
Size: 1572 3325
ApaLI G'TGCA_C
Cuts at: 2954 4110 2954
Size: 1156 3741
ApoI r'AATT_y
Cuts at: 1493 2060 2206 2432 2584 4693 1493
Size: 567 146 226 152 2109 1697
Fragments arranged by size:
2109 1697 567 226 152 146
AseI AT'TA_AT
Cuts at: 160 1032 3451 160
Size: 872 2419 1606
Fragments arranged by size:
2419 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4897
AvaI C'yCGr_G
Cuts at: 1870 1943 2222 1870
Size: 73 279 4545
Fragments arranged by size:
4545 279 73
BamHI G'GATC_C
Cuts at: 2227 2594 2227
Size: 367 4530
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 3673 618
Size: 325 217 627 431 1455 1842
Fragments arranged by size:
1842 1455 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 2216 1391
Size: 825 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3926
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2021 2055 3088 3122 1903
Size: 34 84 34 1033 34 3678
Fragments arranged by size:
3678 1033 84 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2789 3999 2789
Size: 1210 3687
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4897
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3508 136
Size: 107 122 71 631 605 1836 1525
Fragments arranged by size:
1836 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4892 4892
Size: 4897
BlpI GC'TnA_GC
Cuts at: 2089 2314 2089
Size: 225 4672
Bme1580I G_kGCm'C
Cuts at: 1790 2958 4114 1790
Size: 1168 1156 2573
Fragments arranged by size:
2573 1168 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4897
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 3586 442
Size: 416 53 83 399 24 1211 958 1753
Fragments arranged by size:
1753 1211 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4897
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1997 3557 1239
Size: 758 1560 2579
Fragments arranged by size:
2579 1560 758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3022 3887 4185 3022
Size: 865 298 3734
Fragments arranged by size:
3734 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3560 882
Size: 617 2061 2219
Fragments arranged by size:
2219 2061 617
BsaAI yAC'GTr
Cuts at: 493 1204 2688 493
Size: 711 1484 2702
Fragments arranged by size:
2702 1484 711
BsaBI GATnn'nnATC
Cuts at: 4891 4891
Size: 4897
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2704 3086 3721 275
Size: 53 83 186 936 1171 382 635 1451
Fragments arranged by size:
1451 1171 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3330 3728 4002 4149 4560 3330
Size: 398 274 147 411 3667
Fragments arranged by size:
3667 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2028 2058 1178
Size: 30 165 30 625 30 4017
Fragments arranged by size:
4017 625 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4897
BseYI C'CCAG_C
Cuts at: 2175 4100 4596 2175
Size: 1925 496 2476
Fragments arranged by size:
2476 1925 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4324
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2347 3108 4136 1540
Size: 275 186 346 761 1028 2301
Fragments arranged by size:
2301 1028 761 346 275 186
BsiHKAI G_wGCw'C
Cuts at: 2169 2958 3043 4114 2169
Size: 789 85 1071 2952
Fragments arranged by size:
2952 1071 789 85
BsmI GAATG_Cn'
Cuts at: 2408 2501 2408
Size: 93 4804
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2216 2958 3043 4114 1391
Size: 399 379 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 379 85 47
BspEI T'CCGG_A
Cuts at: 4560 4560
Size: 4897
BspHI T'CATG_A
Cuts at: 2169 2786 4516 2169
Size: 617 1730 2550
Fragments arranged by size:
2550 1730 617
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2093 2252 844
Size: 1249 159 3489
Fragments arranged by size:
3489 1249 159
BsrBI CCG'CTC
Cuts at: 1394 1823 2784 1394
Size: 429 961 3507
Fragments arranged by size:
3507 961 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3392 3566 65
Size: 1183 2144 174 1396
Fragments arranged by size:
2144 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3541 3728 1700
Size: 110 1731 187 2869
Fragments arranged by size:
2869 1731 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4897
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4897
BssSI C'ACGA_G
Cuts at: 2957 3969 2957
Size: 1012 3885
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4897
BstBI TT'CG_AA
Cuts at: 3701 3701
Size: 4897
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4897
BstYI r'GATC_y
Cuts at: 1972 2227 2594 2979 2996 4448 4534 4546 4892
Size: 255 367 385 17 1452 86 12 346
Cuts at: 4892 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 255 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4345
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4158
Fragments arranged by size:
4158 683 56
BtsI GCAGTG_nn'
Cuts at: 2413 3207 3227 2413
Size: 794 20 4083
Fragments arranged by size:
4083 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4897
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4862
DraI TTT'AAA
Cuts at: 2201 2201
Size: 4897
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3904 809
Size: 507 2588 1802
Fragments arranged by size:
2588 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2344 3233 4862
Size: 54 1425 50 275 18 514 889 1629
Cuts at: 4862 8
Size: 43
Fragments arranged by size:
1629 1425 889 514 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2344 1537
Size: 275 532 4090
Fragments arranged by size:
4090 532 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2827 1353
Size: 1474 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3484 3858 4004 387
Size: 1064 138 1895 374 146 1280
Fragments arranged by size:
1895 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1997 2075 2960 3557 4344 4612 1239
Size: 393 365 78 885 597 787 268 1524
Fragments arranged by size:
1524 885 787 597 393 365 268 78
EcoRI G'AATT_C
Cuts at: 2206 2584 2206
Size: 378 4519
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 4897
FspI TGC'GCA
Cuts at: 3403 3403
Size: 4897
HaeII r_GCGC'y
Cuts at: 1678 4044 1678
Size: 2366 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2028 2060 2111 2124 2143
Size: 32 135 32 456 32 51 13 19
Cuts at: 2143 2156 3538 3570 3612 3644 1373
Size: 13 1382 32 42 32 2626
Fragments arranged by size:
2626 1382 456 135 51 42 32 32
32 32 32 19 13 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2241 2487 669
Size: 988 281 303 246 3079
Fragments arranged by size:
3079 988 303 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4897
HpaI GTT'AAC
Cuts at: 2487 2487
Size: 4897
KpnI G_GTAC'C
Cuts at: 2222 2222
Size: 4897
MfeI C'AATT_G
Cuts at: 2496 2496
Size: 4897
MluI A'CGCG_T
Cuts at: 4773 4773
Size: 4897
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4011 4195 1305
Size: 2706 184 2007
Fragments arranged by size:
2706 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3075
Fragments arranged by size:
3075 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 3214 3373 518
Size: 648 1689 359 159 2042
Fragments arranged by size:
2042 1689 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2313 2990 4138 4383 1606
Size: 707 677 1148 245 2120
Fragments arranged by size:
2120 1148 707 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4345
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4897
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4897
NotI GC'GGCC_GC
Cuts at: 2344 2344
Size: 4897
NspI r_CATG'y
Cuts at: 1132 1229 2255 3800 1132
Size: 97 1026 1545 2229
Fragments arranged by size:
2229 1545 1026 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4897
PciI A'CATG_T
Cuts at: 3796 3796
Size: 4897
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4897
PmeI GTTT'AAAC
Cuts at: 2201 2201
Size: 4897
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2966 2998 4479 4511 2966
Size: 32 1481 32 3352
Fragments arranged by size:
3352 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4897
PsiI TTA'TAA
Cuts at: 2467 2467
Size: 4897
PstI C_TGCA'G
Cuts at: 830 2249 830
Size: 1419 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4897
SalI G'TCGA_C
Cuts at: 1936 2239 1936
Size: 303 4594
SbfI CC_TGCA'GG
Cuts at: 2249 2249
Size: 4897
ScaI AGT'ACT
Cuts at: 3145 4857 3145
Size: 1712 3185
SfcI C'TryA_G
Cuts at: 826 1045 2245 3380 4061 4252 826
Size: 219 1200 1135 681 191 1471
Fragments arranged by size:
1471 1200 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4897
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4897
SmaI CCC'GGG
Cuts at: 1872 2224 1872
Size: 352 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3001 3902 4164 820
Size: 197 926 1058 901 262 1553
Fragments arranged by size:
1553 1058 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2688 493
Size: 2195 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4897
SphI G_CATG'C
Cuts at: 1132 2255 1132
Size: 1123 3774
SspI AAT'ATT
Cuts at: 5 52 2821 5
Size: 47 2769 2081
Fragments arranged by size:
2769 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4897
StyI C'CwwG_G
Cuts at: 513 1065 2336 513
Size: 552 1271 3074
Fragments arranged by size:
3074 1271 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2924 2941 3094 3279 3724 325
Size: 1255 1344 17 153 185 445 1498
Fragments arranged by size:
1498 1344 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3143 4855 96
Size: 274 80 33 51 1443 1166 1712 138
Fragments arranged by size:
1712 1443 1166 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4857 4889 4857
Size: 32 4865
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4897
XbaI T'CTAG_A
Cuts at: 2233 2233
Size: 4897
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4897
XmaI C'CCGG_G
Cuts at: 1870 2222 1870
Size: 352 4545
XmnI GAAnn'nnTTC
Cuts at: 2156 3026 2156
Size: 870 4027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2705 276
Size: 53 83 186 936 1171 2468
Fragments arranged by size:
2468 1171 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI