Restriction map for
pFN21AE6524
(Circular) (Six-base) MAPSORT of: pFN21AE6524.seq Check: 4128 from: 1 to: 5095
pFN21AE6524 5095 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2167 2167
Size: 5095
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2905 278
Size: 53 83 186 936 1369 2468
Fragments arranged by size:
2468 1369 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2438 1937
Size: 501 4594
Acc65I G'GTAC_C
Cuts at: 2416 2416
Size: 5095
AclI AA'CG_TT
Cuts at: 1563 3222 3595 1563
Size: 1659 373 3063
Fragments arranged by size:
3063 1659 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3158 4542 4810 1632
Size: 1526 1384 268 1917
Fragments arranged by size:
1917 1526 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4898
AflIII A'CryG_T
Cuts at: 1203 3994 4971 1203
Size: 2791 977 1327
Fragments arranged by size:
2791 1327 977
AgeI A'CCGG_T
Cuts at: 3926 3926
Size: 5095
AhdI GACnn_n'nnGTC
Cuts at: 2273 3824 2273
Size: 1551 3544
AleI CACnn'nnGTG
Cuts at: 1166 2209 1166
Size: 1043 4052
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5095
AlwNI CAG_nnn'CTG
Cuts at: 2019 2838 4410 2019
Size: 819 1572 2704
Fragments arranged by size:
2704 1572 819
ApaI G_GGCC'C
Cuts at: 2188 2188
Size: 5095
ApaLI G'TGCA_C
Cuts at: 3152 4308 3152
Size: 1156 3939
ApoI r'AATT_y
Cuts at: 1493 2404 2630 2782 4891 1493
Size: 911 226 152 2109 1697
Fragments arranged by size:
2109 1697 911 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3649 160
Size: 872 2617 1606
Fragments arranged by size:
2617 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5095
AvaI C'yCGr_G
Cuts at: 1870 1943 2164 2420 1870
Size: 73 221 256 4545
Fragments arranged by size:
4545 256 221 73
BamHI G'GATC_C
Cuts at: 2425 2792 2425
Size: 367 4728
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2416 3871 618
Size: 325 217 627 629 1455 1842
Fragments arranged by size:
1842 1455 629 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2188 2414 1391
Size: 797 226 4072
Fragments arranged by size:
4072 797 226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2368 928
Size: 971 469 3655
Fragments arranged by size:
3655 971 469
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3286 3320 1903
Size: 34 1349 34 3678
Fragments arranged by size:
3678 1349 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2987 4197 2987
Size: 1210 3885
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3706 136
Size: 107 122 71 631 605 2034 1525
Fragments arranged by size:
2034 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2136 5090 2136
Size: 2954 2141
BlpI GC'TnA_GC
Cuts at: 2512 2512
Size: 5095
Bme1580I G_kGCm'C
Cuts at: 1790 2188 3156 4312 1790
Size: 398 968 1156 2573
Fragments arranged by size:
2573 1156 968 398
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5095
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2826 3784 442
Size: 416 53 83 399 24 1409 958 1753
Fragments arranged by size:
1753 1409 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2118 2351 3755 1239
Size: 879 233 1404 2579
Fragments arranged by size:
2579 1404 879 233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3220 4085 4383 3220
Size: 865 298 3932
Fragments arranged by size:
3932 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3758 882
Size: 617 2259 2219
Fragments arranged by size:
2259 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2886 493
Size: 711 1682 2702
Fragments arranged by size:
2702 1682 711
BsaBI GATnn'nnATC
Cuts at: 5089 5089
Size: 5095
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2902 3284 3919 275
Size: 53 83 186 936 1369 382 635 1451
Fragments arranged by size:
1451 1369 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2173 3528 3926 4200 4347 4758 2173
Size: 1355 398 274 147 411 2510
Fragments arranged by size:
2510 1355 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 1178
Size: 30 165 30 606 30 4234
Fragments arranged by size:
4234 606 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 1186
Size: 996 4099
BseYI C'CCAG_C
Cuts at: 2187 4298 4794 2187
Size: 2111 496 2488
Fragments arranged by size:
2488 2111 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2545 3306 4334 1540
Size: 275 186 544 761 1028 2301
Fragments arranged by size:
2301 1028 761 544 275 186
BsiHKAI G_wGCw'C
Cuts at: 3156 3241 4312 3156
Size: 85 1071 3939
Fragments arranged by size:
3939 1071 85
BsmI GAATG_Cn'
Cuts at: 2606 2699 2606
Size: 93 5002
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2414 3156 3241 4312 1391
Size: 399 398 226 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 226 85
BspEI T'CCGG_A
Cuts at: 4758 4758
Size: 5095
BspHI T'CATG_A
Cuts at: 2984 4714 2984
Size: 1730 3365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2167 2450 844
Size: 1323 283 3489
Fragments arranged by size:
3489 1323 283
BsrBI CCG'CTC
Cuts at: 1394 1823 2982 1394
Size: 429 1159 3507
Fragments arranged by size:
3507 1159 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3590 3764 65
Size: 1183 2342 174 1396
Fragments arranged by size:
2342 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3739 3926 1700
Size: 110 1929 187 2869
Fragments arranged by size:
2869 1929 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5095
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5095
BssSI C'ACGA_G
Cuts at: 3155 4167 3155
Size: 1012 4083
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2019 794
Size: 1225 3870
BstBI TT'CG_AA
Cuts at: 3899 3899
Size: 5095
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5095
BstXI CCAn_nnnn'nTGG
Cuts at: 2319 2319
Size: 5095
BstYI r'GATC_y
Cuts at: 1972 2136 2425 2792 3177 3194 4646 4732 4744
Size: 164 289 367 385 17 1452 86 12
Cuts at: 4744 5090 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 289 164 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3604
Fragments arranged by size:
3604 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4356
Fragments arranged by size:
4356 683 56
BtsI GCAGTG_nn'
Cuts at: 2017 2611 3405 3425 2017
Size: 594 794 20 3687
Fragments arranged by size:
3687 794 594 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5095
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5060
DraI TTT'AAA
Cuts at: 2399 2399
Size: 5095
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4102 809
Size: 507 2786 1802
Fragments arranged by size:
2786 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2321 2542 3431
Size: 54 1425 50 275 18 491 221 889
Cuts at: 3431 5060 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 491 275 221 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2542 1537
Size: 275 730 4090
Fragments arranged by size:
4090 730 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3025 1353
Size: 720 952 3423
Fragments arranged by size:
3423 952 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3682 4056 4202 387
Size: 1064 138 2093 374 146 1280
Fragments arranged by size:
2093 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2351 3158 3755 4542 4810 1239
Size: 393 486 233 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 486 393 268 233
EcoO109I rG'GnC_Cy
Cuts at: 2184 2184
Size: 5095
EcoRI G'AATT_C
Cuts at: 2404 2782 2404
Size: 378 4717
FspI TGC'GCA
Cuts at: 3601 3601
Size: 5095
HaeII r_GCGC'y
Cuts at: 1678 4242 1678
Size: 2564 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 3736 3768 3810
Size: 32 135 32 687 32 1445 32 42
Cuts at: 3810 3842 1373
Size: 32 2626
Fragments arranged by size:
2626 1445 687 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2439 2685 669
Size: 988 281 501 246 3079
Fragments arranged by size:
3079 988 501 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5095
HpaI GTT'AAC
Cuts at: 2685 2685
Size: 5095
KpnI G_GTAC'C
Cuts at: 2420 2420
Size: 5095
MfeI C'AATT_G
Cuts at: 2694 2694
Size: 5095
MluI A'CGCG_T
Cuts at: 4971 4971
Size: 5095
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4209 4393 1305
Size: 2904 184 2007
Fragments arranged by size:
2904 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2323 10
Size: 54 1425 343 491 2782
Fragments arranged by size:
2782 1425 491 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2209 3053 3412 3571 518
Size: 648 1043 844 359 159 2042
Fragments arranged by size:
2042 1043 844 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2511 3188 4336 4581 1606
Size: 905 677 1148 245 2120
Fragments arranged by size:
2120 1148 905 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3604
Fragments arranged by size:
3604 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5095
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5095
NotI GC'GGCC_GC
Cuts at: 2542 2542
Size: 5095
NspI r_CATG'y
Cuts at: 1132 1229 2453 3998 1132
Size: 97 1224 1545 2229
Fragments arranged by size:
2229 1545 1224 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5095
PciI A'CATG_T
Cuts at: 3994 3994
Size: 5095
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5095
PfoI T'CCnGG_A
Cuts at: 2095 2095
Size: 5095
PmeI GTTT'AAAC
Cuts at: 2399 2399
Size: 5095
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3164 3196 4677 4709 3164
Size: 32 1481 32 3550
Fragments arranged by size:
3550 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5095
PsiI TTA'TAA
Cuts at: 2665 2665
Size: 5095
PspOMI G'GGCC_C
Cuts at: 2184 2184
Size: 5095
PstI C_TGCA'G
Cuts at: 830 2447 830
Size: 1617 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5095
SalI G'TCGA_C
Cuts at: 1936 2437 1936
Size: 501 4594
SbfI CC_TGCA'GG
Cuts at: 2447 2447
Size: 5095
ScaI AGT'ACT
Cuts at: 3343 5055 3343
Size: 1712 3383
SfcI C'TryA_G
Cuts at: 826 1045 2443 3578 4259 4450 826
Size: 219 1398 1135 681 191 1471
Fragments arranged by size:
1471 1398 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5095
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5095
SmaI CCC'GGG
Cuts at: 1872 2422 1872
Size: 550 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3199 4100 4362 820
Size: 197 926 1256 901 262 1553
Fragments arranged by size:
1553 1256 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2886 493
Size: 2393 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5095
SphI G_CATG'C
Cuts at: 1132 2453 1132
Size: 1321 3774
SspI AAT'ATT
Cuts at: 5 52 3019 5
Size: 47 2967 2081
Fragments arranged by size:
2967 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5095
StyI C'CwwG_G
Cuts at: 513 1065 2004 2534 513
Size: 552 939 530 3074
Fragments arranged by size:
3074 939 552 530
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2221 3122 3139 3292 3477 3922 325
Size: 1255 641 901 17 153 185 445 1498
Fragments arranged by size:
1498 1255 901 641 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3341 5053 96
Size: 274 80 33 51 1443 1364 1712 138
Fragments arranged by size:
1712 1443 1364 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2009 2041 5055 5087 2009
Size: 32 3014 32 2017
Fragments arranged by size:
3014 2017 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5095
XbaI T'CTAG_A
Cuts at: 2431 2431
Size: 5095
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5095
XmaI C'CCGG_G
Cuts at: 1870 2420 1870
Size: 550 4545
XmnI GAAnn'nnTTC
Cuts at: 3224 3224
Size: 5095
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2903 276
Size: 53 83 186 936 1369 2468
Fragments arranged by size:
2468 1369 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI