Restriction map for
pFN21AE6523
(Circular) (Six-base) MAPSORT of: pFN21AE6523.seq Check: 9045 from: 1 to: 4987
pFN21AE6523 4987 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2797 278
Size: 53 83 186 936 1261 2468
Fragments arranged by size:
2468 1261 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2330 1937
Size: 393 4594
Acc65I G'GTAC_C
Cuts at: 2308 2308
Size: 4987
AclI AA'CG_TT
Cuts at: 1563 3114 3487 1563
Size: 1551 373 3063
Fragments arranged by size:
3063 1551 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2167 3050 4434 4702 1632
Size: 535 883 1384 268 1917
Fragments arranged by size:
1917 1384 883 535 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4790
AflIII A'CryG_T
Cuts at: 1203 3886 4863 1203
Size: 2683 977 1327
Fragments arranged by size:
2683 1327 977
AgeI A'CCGG_T
Cuts at: 3818 3818
Size: 4987
AhdI GACnn_n'nnGTC
Cuts at: 3716 3716
Size: 4987
AleI CACnn'nnGTG
Cuts at: 1166 2062 1166
Size: 896 4091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4987
AlwNI CAG_nnn'CTG
Cuts at: 2092 2730 4302 2092
Size: 638 1572 2777
Fragments arranged by size:
2777 1572 638
ApaLI G'TGCA_C
Cuts at: 3044 4200 3044
Size: 1156 3831
ApoI r'AATT_y
Cuts at: 1493 2296 2522 2674 4783 1493
Size: 803 226 152 2109 1697
Fragments arranged by size:
2109 1697 803 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3541 160
Size: 872 2509 1606
Fragments arranged by size:
2509 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4987
AvaI C'yCGr_G
Cuts at: 1870 1943 2312 1870
Size: 73 369 4545
Fragments arranged by size:
4545 369 73
BamHI G'GATC_C
Cuts at: 2317 2684 2317
Size: 367 4620
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 2308 3763 618
Size: 325 217 627 415 106 1455 1842
Fragments arranged by size:
1842 1455 627 415 325 217 106
BanII G_rGCy'C
Cuts at: 1391 2081 2255 2306 1391
Size: 690 174 51 4072
Fragments arranged by size:
4072 690 174 51
BbeI G_GCGC'C
Cuts at: 2206 2206
Size: 4987
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4016
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3178 3212 1903
Size: 34 1241 34 3678
Fragments arranged by size:
3678 1241 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2879 4089 2879
Size: 1210 3777
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4987
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3598 136
Size: 107 122 71 631 605 1926 1525
Fragments arranged by size:
1926 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4982 4982
Size: 4987
BlpI GC'TnA_GC
Cuts at: 2404 2404
Size: 4987
Bme1580I G_kGCm'C
Cuts at: 1790 3048 4204 1790
Size: 1258 1156 2573
Fragments arranged by size:
2573 1258 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4987
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2040 2136 2718
Size: 416 53 83 399 24 623 96 582
Cuts at: 2718 3676 442
Size: 958 1753
Fragments arranged by size:
1753 958 623 582 416 399 96 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3647 1239
Size: 2408 2579
Bpu10I CC'TnA_GC
Cuts at: 2165 2165
Size: 4987
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3112 3977 4275 3112
Size: 865 298 3824
Fragments arranged by size:
3824 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3650 882
Size: 617 2151 2219
Fragments arranged by size:
2219 2151 617
BsaAI yAC'GTr
Cuts at: 493 1204 2778 493
Size: 711 1574 2702
Fragments arranged by size:
2702 1574 711
BsaBI GATnn'nnATC
Cuts at: 4981 4981
Size: 4987
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2203 2794 3176 3811
Size: 53 83 186 936 670 591 382 635
Cuts at: 3811 275
Size: 1451
Fragments arranged by size:
1451 936 670 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3420 3818 4092 4239 4650 3420
Size: 398 274 147 411 3757
Fragments arranged by size:
3757 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4762
Fragments arranged by size:
4762 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4987
BseYI C'CCAG_C
Cuts at: 2080 4190 4686 2080
Size: 2110 496 2381
Fragments arranged by size:
2381 2110 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4414
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2437 3198 4226 1540
Size: 275 186 436 761 1028 2301
Fragments arranged by size:
2301 1028 761 436 275 186
BsiHKAI G_wGCw'C
Cuts at: 2119 3048 3133 4204 2119
Size: 929 85 1071 2902
Fragments arranged by size:
2902 1071 929 85
BsmI GAATG_Cn'
Cuts at: 2498 2591 2498
Size: 93 4894
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2119 2255 2306 3048 3133 4204
Size: 399 291 38 136 51 742 85 1071
Cuts at: 4204 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 291 136 85 51
38
BspEI T'CCGG_A
Cuts at: 4650 4650
Size: 4987
BspHI T'CATG_A
Cuts at: 2876 4606 2876
Size: 1730 3257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2342 844
Size: 1498 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2874 1394
Size: 429 1051 3507
Fragments arranged by size:
3507 1051 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3482 3656 65
Size: 1183 2234 174 1396
Fragments arranged by size:
2234 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 3631 3818 1700
Size: 110 389 1432 187 2869
Fragments arranged by size:
2869 1432 389 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4987
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4987
BssSI C'ACGA_G
Cuts at: 3047 4059 3047
Size: 1012 3975
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4987
BstBI TT'CG_AA
Cuts at: 3791 3791
Size: 4987
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4987
BstXI CCAn_nnnn'nTGG
Cuts at: 2273 2273
Size: 4987
BstYI r'GATC_y
Cuts at: 1972 2210 2317 2684 3069 3086 4538 4624 4636
Size: 238 107 367 385 17 1452 86 12
Cuts at: 4636 4982 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 238 107 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2260 2266 513
Size: 552 1195 6 3234
Fragments arranged by size:
3234 1195 552 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4248
Fragments arranged by size:
4248 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 2239 2503 3297 3317 2036
Size: 203 264 794 20 3706
Fragments arranged by size:
3706 794 264 203 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4987
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4952
DraI TTT'AAA
Cuts at: 2291 2291
Size: 4987
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3994 809
Size: 507 2678 1802
Fragments arranged by size:
2678 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2263 2434 3323
Size: 54 1425 50 275 18 433 171 889
Cuts at: 3323 4952 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 433 275 171 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2434 1537
Size: 275 622 4090
Fragments arranged by size:
4090 622 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2110 2917 1353
Size: 757 807 3423
Fragments arranged by size:
3423 807 757
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3574 3948 4094 387
Size: 1064 138 1985 374 146 1280
Fragments arranged by size:
1985 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2167 3050 3647 4434 4702 1239
Size: 393 535 883 597 787 268 1524
Fragments arranged by size:
1524 883 787 597 535 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2224 2224
Size: 4987
EcoO109I rG'GnC_Cy
Cuts at: 2187 2187
Size: 4987
EcoRI G'AATT_C
Cuts at: 2296 2674 2296
Size: 378 4609
FspI TGC'GCA
Cuts at: 3493 3493
Size: 4987
HaeII r_GCGC'y
Cuts at: 1678 2206 4134 1678
Size: 528 1928 2531
Fragments arranged by size:
2531 1928 528
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3628 3660 3702 3734 1373
Size: 32 135 32 2056 32 42 32 2626
Fragments arranged by size:
2626 2056 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2331 2577 669
Size: 988 281 393 246 3079
Fragments arranged by size:
3079 988 393 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4987
HpaI GTT'AAC
Cuts at: 2577 2577
Size: 4987
KasI G'GCGC_C
Cuts at: 2202 2202
Size: 4987
KpnI G_GTAC'C
Cuts at: 2312 2312
Size: 4987
MfeI C'AATT_G
Cuts at: 2586 2586
Size: 4987
MluI A'CGCG_T
Cuts at: 4863 4863
Size: 4987
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2238 4101 4285 1305
Size: 933 1863 184 2007
Fragments arranged by size:
2007 1863 933 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2265 10
Size: 54 1425 343 433 2732
Fragments arranged by size:
2732 1425 433 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2062 2945 3304 3463 518
Size: 648 896 883 359 159 2042
Fragments arranged by size:
2042 896 883 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2403 3080 4228 4473 1606
Size: 797 677 1148 245 2120
Fragments arranged by size:
2120 1148 797 677 245
NaeI GCC'GGC
Cuts at: 2201 2201
Size: 4987
NarI GG'CG_CC
Cuts at: 2203 2203
Size: 4987
NcoI C'CATG_G
Cuts at: 513 1065 2266 513
Size: 552 1201 3234
Fragments arranged by size:
3234 1201 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4987
NgoMIV G'CCGG_C
Cuts at: 2199 2199
Size: 4987
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4987
NotI GC'GGCC_GC
Cuts at: 2434 2434
Size: 4987
NspI r_CATG'y
Cuts at: 1132 1229 2345 3890 1132
Size: 97 1116 1545 2229
Fragments arranged by size:
2229 1545 1116 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4987
PciI A'CATG_T
Cuts at: 3886 3886
Size: 4987
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4987
PmeI GTTT'AAAC
Cuts at: 2291 2291
Size: 4987
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 4569 4601 3056
Size: 32 1481 32 3442
Fragments arranged by size:
3442 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2187 2187
Size: 4987
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4987
PsiI TTA'TAA
Cuts at: 2557 2557
Size: 4987
PstI C_TGCA'G
Cuts at: 830 2339 830
Size: 1509 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4987
SalI G'TCGA_C
Cuts at: 1936 2329 1936
Size: 393 4594
SbfI CC_TGCA'GG
Cuts at: 2339 2339
Size: 4987
ScaI AGT'ACT
Cuts at: 3235 4947 3235
Size: 1712 3275
SfcI C'TryA_G
Cuts at: 826 1045 2335 3470 4151 4342 826
Size: 219 1290 1135 681 191 1471
Fragments arranged by size:
1471 1290 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2204 2204
Size: 4987
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4987
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4987
SmaI CCC'GGG
Cuts at: 1872 2314 1872
Size: 442 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3091 3992 4254 820
Size: 197 926 1148 901 262 1553
Fragments arranged by size:
1553 1148 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2778 493
Size: 2285 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4987
SphI G_CATG'C
Cuts at: 1132 2345 1132
Size: 1213 3774
SspI AAT'ATT
Cuts at: 5 52 2911 5
Size: 47 2859 2081
Fragments arranged by size:
2859 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4987
StyI C'CwwG_G
Cuts at: 513 1065 2266 2426 513
Size: 552 1201 160 3074
Fragments arranged by size:
3074 1201 552 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2074 3014 3031 3184 3369 3814 325
Size: 1255 494 940 17 153 185 445 1498
Fragments arranged by size:
1498 1255 940 494 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3233 4945 96
Size: 274 80 33 51 1443 1256 1712 138
Fragments arranged by size:
1712 1443 1256 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2171 2203 4947 4979 2171
Size: 32 2744 32 2179
Fragments arranged by size:
2744 2179 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4987
XbaI T'CTAG_A
Cuts at: 2323 2323
Size: 4987
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4987
XmaI C'CCGG_G
Cuts at: 1870 2312 1870
Size: 442 4545
XmnI GAAnn'nnTTC
Cuts at: 3116 3116
Size: 4987
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2795 276
Size: 53 83 186 936 1261 2468
Fragments arranged by size:
2468 1261 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI