Restriction map for
pFN21AE6521
(Circular) (Six-base) MAPSORT of: pFN21AE6521.seq Check: 1723 from: 1 to: 5230
pFN21AE6521 5230 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2523 3293 4677 4945 1632
Size: 891 770 1384 268 1917
Fragments arranged by size:
1917 1384 891 770 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 2261 4129 5106 1203
Size: 1058 1868 977 1327
Fragments arranged by size:
1868 1327 1058 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5230
AlwNI CAG_nnn'CTG
Cuts at: 2973 4545 2973
Size: 1572 3658
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2035 2279 2343 2452 2539 2765 2917 5026
Size: 542 244 64 109 87 226 152 2109
Cuts at: 5026 1493
Size: 1697
Fragments arranged by size:
2109 1697 542 244 226 152 109 87
64
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BamHI G'GATC_C
Cuts at: 2560 2927 2560
Size: 367 4863
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2549 1391
Size: 1158 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2226 2511 928
Size: 971 327 285 3647
Fragments arranged by size:
3647 971 327 285
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3122 4332 3122
Size: 1210 4020
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3841 136
Size: 107 122 71 631 605 2169 1525
Fragments arranged by size:
2169 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3919 442
Size: 416 53 83 399 24 1544 958 1753
Fragments arranged by size:
1753 1544 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3890 1239
Size: 2651 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3355 4220 4518 3355
Size: 865 298 4067
Fragments arranged by size:
4067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3893 882
Size: 617 2394 2219
Fragments arranged by size:
2394 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3037 3419 4054 275
Size: 53 83 186 936 1504 382 635 1451
Fragments arranged by size:
1504 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2001 2031 1178
Size: 30 165 30 598 30 4377
Fragments arranged by size:
4377 598 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5230
BseYI C'CCAG_C
Cuts at: 4433 4929 4433
Size: 496 4734
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4657
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 3441 4469 1540
Size: 275 186 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 2014 3291 3376 4447 2014
Size: 1277 85 1071 2797
Fragments arranged by size:
2797 1277 1071 85
BsmI GAATG_Cn'
Cuts at: 2328 2741 2834 2328
Size: 413 93 4724
Fragments arranged by size:
4724 413 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2549 3291 3376 4447 1391
Size: 399 224 535 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 535 399 224 85
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2233 2585 844
Size: 1389 352 3489
Fragments arranged by size:
3489 1389 352
BsrBI CCG'CTC
Cuts at: 1394 1823 2057 3117 1394
Size: 429 234 1060 3507
Fragments arranged by size:
3507 1060 429 234
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3874 4061 1700
Size: 110 2064 187 2869
Fragments arranged by size:
2869 2064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5230
BstXI CCAn_nnnn'nTGG
Cuts at: 2400 2400
Size: 5230
BstYI r'GATC_y
Cuts at: 1972 2224 2560 2927 3312 3329 4781 4867 4879
Size: 252 336 367 385 17 1452 86 12
Cuts at: 4879 5225 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 336 252 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4491
Fragments arranged by size:
4491 683 56
BtsI GCAGTG_nn'
Cuts at: 2746 3540 3560 2746
Size: 794 20 4416
Fragments arranged by size:
4416 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5195
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2677 3566 5195
Size: 54 1425 50 275 18 847 889 1629
Cuts at: 5195 8
Size: 43
Fragments arranged by size:
1629 1425 889 847 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 1537
Size: 275 865 4090
Fragments arranged by size:
4090 865 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2463 3160 1353
Size: 1110 697 3423
Fragments arranged by size:
3423 1110 697
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3817 4191 4337 387
Size: 1064 138 2228 374 146 1280
Fragments arranged by size:
2228 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2523 3293 3890 4677 4945 1239
Size: 393 891 770 597 787 268 1524
Fragments arranged by size:
1524 891 787 770 597 393 268
EcoRI G'AATT_C
Cuts at: 2279 2452 2539 2917 2279
Size: 173 87 378 4592
Fragments arranged by size:
4592 378 173 87
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 4377 1678
Size: 2699 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2216 2248 3871 3903 3945
Size: 32 135 32 644 32 1623 32 42
Cuts at: 3945 3977 1373
Size: 32 2626
Fragments arranged by size:
2626 1623 644 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2574 2820 669
Size: 988 281 636 246 3079
Fragments arranged by size:
3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 2384 748
Size: 1636 3594
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4344 4528 1305
Size: 3039 184 2007
Fragments arranged by size:
3039 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3547 3706 518
Size: 648 2022 359 159 2042
Fragments arranged by size:
2042 2022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2646 3323 4471 4716 1606
Size: 1040 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2265 2588 4133 1132
Size: 97 1036 323 1545 2229
Fragments arranged by size:
2229 1545 1036 323 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 2261 4129 2261
Size: 1868 3362
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 3478 5190 3478
Size: 1712 3518
SfcI C'TryA_G
Cuts at: 826 1045 2578 3713 4394 4585 826
Size: 219 1533 1135 681 191 1471
Fragments arranged by size:
1533 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3334 4235 4497 820
Size: 197 926 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 2014 152
Size: 1862 3368
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 2320 2468 3154 5
Size: 47 2268 148 686 2081
Fragments arranged by size:
2268 2081 686 148 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2669 513
Size: 552 939 665 3074
Fragments arranged by size:
3074 939 665 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3274 3427 3612 4057 325
Size: 1255 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3476 5188 96
Size: 274 80 33 51 1443 1499 1712 138
Fragments arranged by size:
1712 1499 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2001 2033 5190 5222 2001
Size: 32 3157 32 2009
Fragments arranged by size:
3157 2009 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5230
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 2279 3359 2279
Size: 1080 4150
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI