Restriction map for
pFN21AE6518
(Circular) (Six-base) MAPSORT of: pFN21AE6518.seq Check: 8883 from: 1 to: 5290
pFN21AE6518 5290 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3100 278
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2633 1937
Size: 696 4594
Acc65I G'GTAC_C
Cuts at: 2611 2611
Size: 5290
AclI AA'CG_TT
Cuts at: 1563 3417 3790 1563
Size: 1854 373 3063
Fragments arranged by size:
3063 1854 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3353 4737 5005 1632
Size: 1721 1384 268 1917
Fragments arranged by size:
1917 1721 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5093
AflIII A'CryG_T
Cuts at: 1203 4189 5166 1203
Size: 2986 977 1327
Fragments arranged by size:
2986 1327 977
AgeI A'CCGG_T
Cuts at: 4121 4121
Size: 5290
AhdI GACnn_n'nnGTC
Cuts at: 4019 4019
Size: 5290
AleI CACnn'nnGTG
Cuts at: 1166 2200 1166
Size: 1034 4256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5290
AlwNI CAG_nnn'CTG
Cuts at: 3033 4605 3033
Size: 1572 3718
ApaLI G'TGCA_C
Cuts at: 3347 4503 3347
Size: 1156 4134
ApoI r'AATT_y
Cuts at: 1493 2599 2825 2977 5086 1493
Size: 1106 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3844 160
Size: 872 2812 1606
Fragments arranged by size:
2812 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
AvaI C'yCGr_G
Cuts at: 1870 1943 2615 1870
Size: 73 672 4545
Fragments arranged by size:
4545 672 73
BamHI G'GATC_C
Cuts at: 2620 2987 2620
Size: 367 4923
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2387 2611 4066 618
Size: 325 217 627 600 224 1455 1842
Fragments arranged by size:
1842 1455 627 600 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2028 2316 2609 1391
Size: 637 288 293 4072
Fragments arranged by size:
4072 637 293 288
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2413 2508 928
Size: 971 514 95 3710
Fragments arranged by size:
3710 971 514 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2099 2133 3481 3515 1903
Size: 34 162 34 1348 34 3678
Fragments arranged by size:
3678 1348 162 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3182 4392 3182
Size: 1210 4080
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5290
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2057 3901 136
Size: 107 122 71 631 605 385 1844 1525
Fragments arranged by size:
1844 1525 631 605 385 122 107 71
BglII A'GATC_T
Cuts at: 5285 5285
Size: 5290
BlpI GC'TnA_GC
Cuts at: 2457 2707 2457
Size: 250 5040
Bme1580I G_kGCm'C
Cuts at: 1790 3351 4507 1790
Size: 1561 1156 2573
Fragments arranged by size:
2573 1561 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5290
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3021 3979 442
Size: 416 53 83 399 24 1604 958 1753
Fragments arranged by size:
1753 1604 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5290
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2049 2342 3950 1239
Size: 810 293 1608 2579
Fragments arranged by size:
2579 1608 810 293
Bpu10I CC'TnA_GC
Cuts at: 2085 2091 2148 2085
Size: 6 57 5227
Fragments arranged by size:
5227 57 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3415 4280 4578 3415
Size: 865 298 4127
Fragments arranged by size:
4127 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3953 882
Size: 617 2454 2219
Fragments arranged by size:
2454 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2470 3081 493
Size: 711 1266 611 2702
Fragments arranged by size:
2702 1266 711 611
BsaBI GATnn'nnATC
Cuts at: 2213 5284 2213
Size: 3071 2219
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3097 3479 4114 275
Size: 53 83 186 936 1564 382 635 1451
Fragments arranged by size:
1564 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3723 4121 4395 4542 4953 3723
Size: 398 274 147 411 4060
Fragments arranged by size:
4060 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2096 2126 1178
Size: 30 165 30 693 30 4342
Fragments arranged by size:
4342 693 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5290
BseYI C'CCAG_C
Cuts at: 2251 2482 2525 4493 4989 2251
Size: 231 43 1968 496 2552
Fragments arranged by size:
2552 1968 496 231 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4717
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2191 2740 3501 4529 1540
Size: 275 186 190 549 761 1028 2301
Fragments arranged by size:
2301 1028 761 549 275 190 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2316 3351 3436 4507 2283
Size: 33 1035 85 1071 3066
Fragments arranged by size:
3066 1071 1035 85 33
BsmI GAATG_Cn'
Cuts at: 2801 2894 2801
Size: 93 5197
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2283 2316 2609 3351 3436 4507
Size: 399 238 255 33 293 742 85 1071
Cuts at: 4507 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 293 255 238 85
33
BspEI T'CCGG_A
Cuts at: 4953 4953
Size: 5290
BspHI T'CATG_A
Cuts at: 3179 4909 3179
Size: 1730 3560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2645 844
Size: 1801 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3177 1394
Size: 429 1354 3507
Fragments arranged by size:
3507 1354 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2443 3785 3959 65
Size: 1183 1195 1342 174 1396
Fragments arranged by size:
1396 1342 1195 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3934 4121 1700
Size: 110 2124 187 2869
Fragments arranged by size:
2869 2124 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5290
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5290
BssSI C'ACGA_G
Cuts at: 3350 4362 3350
Size: 1012 4278
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5290
BstBI TT'CG_AA
Cuts at: 4094 4094
Size: 5290
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5290
BstXI CCAn_nnnn'nTGG
Cuts at: 2329 2329
Size: 5290
BstYI r'GATC_y
Cuts at: 1972 2620 2987 3372 3389 4841 4927 4939 5285
Size: 648 367 385 17 1452 86 12 346
Cuts at: 5285 1972
Size: 1977
Fragments arranged by size:
1977 1452 648 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2100 2100
Size: 5290
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3799
Fragments arranged by size:
3799 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4551
Fragments arranged by size:
4551 683 56
BtsI GCAGTG_nn'
Cuts at: 2234 2806 3600 3620 2234
Size: 572 794 20 3904
Fragments arranged by size:
3904 794 572 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5255
DraI TTT'AAA
Cuts at: 2594 2594
Size: 5290
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4297 809
Size: 507 2981 1802
Fragments arranged by size:
2981 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 2737 3626
Size: 54 1425 50 275 18 645 262 889
Cuts at: 3626 5255 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 645 275 262 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2737 1537
Size: 275 925 4090
Fragments arranged by size:
4090 925 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2154 2304 3220 1353
Size: 801 150 916 3423
Fragments arranged by size:
3423 916 801 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3877 4251 4397 387
Size: 1064 138 2288 374 146 1280
Fragments arranged by size:
2288 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2314 2314
Size: 5290
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2342 3353 3950 4737 5005 1239
Size: 393 417 293 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 597 417 393 293 268
EcoRI G'AATT_C
Cuts at: 2599 2977 2599
Size: 378 4912
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 5290
FspI TGC'GCA
Cuts at: 3796 3796
Size: 5290
HaeII r_GCGC'y
Cuts at: 1678 2098 2252 4437 1678
Size: 420 154 2185 2531
Fragments arranged by size:
2531 2185 420 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3931 3963 4005 4037 1373
Size: 32 135 32 2359 32 42 32 2626
Fragments arranged by size:
2626 2359 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2634 2880 669
Size: 988 281 696 246 3079
Fragments arranged by size:
3079 988 696 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5290
HpaI GTT'AAC
Cuts at: 2880 2880
Size: 5290
KpnI G_GTAC'C
Cuts at: 2615 2615
Size: 5290
MfeI C'AATT_G
Cuts at: 2889 2889
Size: 5290
MluI A'CGCG_T
Cuts at: 5166 5166
Size: 5290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4404 4588 1305
Size: 3099 184 2007
Fragments arranged by size:
3099 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2477 10
Size: 54 1425 343 645 2823
Fragments arranged by size:
2823 1425 645 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2200 2447 3248 3607 3766 518
Size: 648 1034 247 801 359 159 2042
Fragments arranged by size:
2042 1034 801 648 359 247 159
MspA1I CmG'CkG
Cuts at: 1606 2706 3383 4531 4776 1606
Size: 1100 677 1148 245 2120
Fragments arranged by size:
2120 1148 1100 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3799
Fragments arranged by size:
3799 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5290
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5290
NotI GC'GGCC_GC
Cuts at: 2737 2737
Size: 5290
NspI r_CATG'y
Cuts at: 1132 1229 2648 4193 1132
Size: 97 1419 1545 2229
Fragments arranged by size:
2229 1545 1419 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5290
PciI A'CATG_T
Cuts at: 4189 4189
Size: 5290
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2473 1108
Size: 1365 3925
PmeI GTTT'AAAC
Cuts at: 2594 2594
Size: 5290
PmlI CAC'GTG
Cuts at: 2470 2470
Size: 5290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3359 3391 4872 4904 3359
Size: 32 1481 32 3745
Fragments arranged by size:
3745 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5290
PsiI TTA'TAA
Cuts at: 2860 2860
Size: 5290
PstI C_TGCA'G
Cuts at: 830 2642 830
Size: 1812 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5290
SacI G_AGCT'C
Cuts at: 2316 2316
Size: 5290
SalI G'TCGA_C
Cuts at: 1936 2632 1936
Size: 696 4594
SapI GCTCTTCn'nnn_
Cuts at: 2304 2304
Size: 5290
SbfI CC_TGCA'GG
Cuts at: 2642 2642
Size: 5290
ScaI AGT'ACT
Cuts at: 3538 5250 3538
Size: 1712 3578
SfcI C'TryA_G
Cuts at: 826 1045 2638 3773 4454 4645 826
Size: 219 1593 1135 681 191 1471
Fragments arranged by size:
1593 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5290
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5290
SmaI CCC'GGG
Cuts at: 1872 2617 1872
Size: 745 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3394 4295 4557 820
Size: 197 926 1451 901 262 1553
Fragments arranged by size:
1553 1451 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3081 493
Size: 2588 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5290
SphI G_CATG'C
Cuts at: 1132 2648 1132
Size: 1516 3774
SspI AAT'ATT
Cuts at: 5 52 3214 5
Size: 47 3162 2081
Fragments arranged by size:
3162 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5290
StyI C'CwwG_G
Cuts at: 513 1065 2004 2043 2055 2729 513
Size: 552 939 39 12 674 3074
Fragments arranged by size:
3074 939 674 552 39 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2441 2474 3317 3334 3487 3672
Size: 1255 632 229 33 843 17 153 185
Cuts at: 3672 4117 325
Size: 445 1498
Fragments arranged by size:
1498 1255 843 632 445 229 185 153
33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3536 5248 96
Size: 274 80 33 51 1443 1559 1712 138
Fragments arranged by size:
1712 1559 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2567 2599 5250 5282 2567
Size: 32 2651 32 2575
Fragments arranged by size:
2651 2575 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5290
XbaI T'CTAG_A
Cuts at: 2626 2626
Size: 5290
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5290
XmaI C'CCGG_G
Cuts at: 1870 2615 1870
Size: 745 4545
XmnI GAAnn'nnTTC
Cuts at: 3419 3419
Size: 5290
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3098 276
Size: 53 83 186 936 1564 2468
Fragments arranged by size:
2468 1564 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI