Restriction map for
pFN21AE6516
(Circular) (Six-base) MAPSORT of: pFN21AE6516.seq Check: 2189 from: 1 to: 5260
pFN21AE6516 5260 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2494 2494
Size: 5260
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3070 278
Size: 53 83 186 936 1534 2468
Fragments arranged by size:
2468 1534 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2603 1937
Size: 666 4594
Acc65I G'GTAC_C
Cuts at: 2581 2581
Size: 5260
AclI AA'CG_TT
Cuts at: 1563 3387 3760 1563
Size: 1824 373 3063
Fragments arranged by size:
3063 1824 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3323 4707 4975 1632
Size: 1691 1384 268 1917
Fragments arranged by size:
1917 1691 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5063
AflIII A'CryG_T
Cuts at: 1203 4159 5136 1203
Size: 2956 977 1327
Fragments arranged by size:
2956 1327 977
AgeI A'CCGG_T
Cuts at: 4091 4091
Size: 5260
AhdI GACnn_n'nnGTC
Cuts at: 3989 3989
Size: 5260
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5260
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5260
AlwNI CAG_nnn'CTG
Cuts at: 2131 3003 4575 2131
Size: 872 1572 2816
Fragments arranged by size:
2816 1572 872
ApaLI G'TGCA_C
Cuts at: 3317 4473 3317
Size: 1156 4104
ApoI r'AATT_y
Cuts at: 1493 2279 2461 2569 2795 2947 5056 1493
Size: 786 182 108 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 226 182 152 108
AseI AT'TA_AT
Cuts at: 160 1032 3814 160
Size: 872 2782 1606
Fragments arranged by size:
2782 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
AvaI C'yCGr_G
Cuts at: 1870 1943 2585 1870
Size: 73 642 4545
Fragments arranged by size:
4545 642 73
BamHI G'GATC_C
Cuts at: 2590 2957 2590
Size: 367 4893
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2501 2581 4036 618
Size: 325 217 627 714 80 1455 1842
Fragments arranged by size:
1842 1455 714 627 325 217 80
BanII G_rGCy'C
Cuts at: 1391 2559 2579 1391
Size: 1168 20 4072
Fragments arranged by size:
4072 1168 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2506 2540 3451 3485 1903
Size: 34 569 34 911 34 3678
Fragments arranged by size:
3678 911 569 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3152 4362 3152
Size: 1210 4050
BclI T'GATC_A
Cuts at: 1548 2319 1548
Size: 771 4489
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2534 3871 136
Size: 107 122 71 631 605 862 1337 1525
Fragments arranged by size:
1525 1337 862 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5255 5255
Size: 5260
BlpI GC'TnA_GC
Cuts at: 2677 2677
Size: 5260
Bme1580I G_kGCm'C
Cuts at: 1790 2415 3321 4477 1790
Size: 625 906 1156 2573
Fragments arranged by size:
2573 1156 906 625
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5260
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2451 2991 3949
Size: 416 53 83 399 24 1034 540 958
Cuts at: 3949 442
Size: 1753
Fragments arranged by size:
1753 1034 958 540 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3920 1239
Size: 2681 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3385 4250 4548 3385
Size: 865 298 4097
Fragments arranged by size:
4097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3923 882
Size: 617 2424 2219
Fragments arranged by size:
2424 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3051 493
Size: 711 1847 2702
Fragments arranged by size:
2702 1847 711
BsaBI GATnn'nnATC
Cuts at: 5254 5254
Size: 5260
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3067 3449 4084 275
Size: 53 83 186 936 1534 382 635 1451
Fragments arranged by size:
1534 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2288 3693 4091 4365 4512 4923 2288
Size: 1405 398 274 147 411 2625
Fragments arranged by size:
2625 1405 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5035
Fragments arranged by size:
5035 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5260
BseYI C'CCAG_C
Cuts at: 2121 4463 4959 2121
Size: 2342 496 2422
Fragments arranged by size:
2422 2342 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4687
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2710 3471 4499 1540
Size: 275 186 709 761 1028 2301
Fragments arranged by size:
2301 1028 761 709 275 186
BsiHKAI G_wGCw'C
Cuts at: 2404 2559 3321 3406 4477 2404
Size: 155 762 85 1071 3187
Fragments arranged by size:
3187 1071 762 155 85
BsmI GAATG_Cn'
Cuts at: 2012 2771 2864 2012
Size: 759 93 4408
Fragments arranged by size:
4408 759 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2404 2415 2559 2579 3321 3406 4477
Size: 399 614 11 144 20 742 85 1071
Cuts at: 4477 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 614 399 144 85 20
11
BspEI T'CCGG_A
Cuts at: 4923 4923
Size: 5260
BspHI T'CATG_A
Cuts at: 3149 4879 3149
Size: 1730 3530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2028 2494 2615 844
Size: 1184 466 121 3489
Fragments arranged by size:
3489 1184 466 121
BsrBI CCG'CTC
Cuts at: 1394 1823 3147 1394
Size: 429 1324 3507
Fragments arranged by size:
3507 1324 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3755 3929 65
Size: 1183 2507 174 1396
Fragments arranged by size:
2507 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3904 4091 1700
Size: 110 2094 187 2869
Fragments arranged by size:
2869 2094 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5260
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5260
BssSI C'ACGA_G
Cuts at: 3320 4332 3320
Size: 1012 4248
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5260
BstBI TT'CG_AA
Cuts at: 4064 4064
Size: 5260
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5260
BstYI r'GATC_y
Cuts at: 1972 2221 2590 2957 3342 3359 4811 4897 4909
Size: 249 369 367 385 17 1452 86 12
Cuts at: 4909 5255 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 369 367 346 249 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2077 2077
Size: 5260
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4708
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4521
Fragments arranged by size:
4521 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 2524 2776 3570 3590 2503
Size: 21 252 794 20 4173
Fragments arranged by size:
4173 794 252 21 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5260
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5225
DraI TTT'AAA
Cuts at: 2564 2564
Size: 5260
DraIII CAC_nnn'GTG
Cuts at: 2317 2374 2317
Size: 57 5203
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4267 809
Size: 507 2951 1802
Fragments arranged by size:
2951 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2707 3596 5225
Size: 54 1425 50 275 18 877 889 1629
Cuts at: 5225 8
Size: 43
Fragments arranged by size:
1629 1425 889 877 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2707 1537
Size: 275 895 4090
Fragments arranged by size:
4090 895 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3190 1353
Size: 1837 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3847 4221 4367 387
Size: 1064 138 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5260
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3323 3920 4707 4975 1239
Size: 393 1691 597 787 268 1524
Fragments arranged by size:
1691 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2569 2947 2569
Size: 378 4882
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2131 2131
Size: 5260
FspI TGC'GCA
Cuts at: 3766 3766
Size: 5260
HaeII r_GCGC'y
Cuts at: 1678 4407 1678
Size: 2729 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3901 3933 3975 4007 1373
Size: 32 135 32 2329 32 42 32 2626
Fragments arranged by size:
2626 2329 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2604 2850 669
Size: 988 281 666 246 3079
Fragments arranged by size:
3079 988 666 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5260
HpaI GTT'AAC
Cuts at: 2850 2850
Size: 5260
KpnI G_GTAC'C
Cuts at: 2585 2585
Size: 5260
MfeI C'AATT_G
Cuts at: 2859 2859
Size: 5260
MluI A'CGCG_T
Cuts at: 5136 5136
Size: 5260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2462 4374 4558 1305
Size: 1157 1912 184 2007
Fragments arranged by size:
2007 1912 1157 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3438
Fragments arranged by size:
3438 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3218 3577 3736 518
Size: 648 2052 359 159 2042
Fragments arranged by size:
2052 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2676 3353 4501 4746 1606
Size: 1070 677 1148 245 2120
Fragments arranged by size:
2120 1148 1070 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4708
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5260
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5260
NotI GC'GGCC_GC
Cuts at: 2707 2707
Size: 5260
NspI r_CATG'y
Cuts at: 1132 1229 2618 4163 1132
Size: 97 1389 1545 2229
Fragments arranged by size:
2229 1545 1389 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5260
PciI A'CATG_T
Cuts at: 4159 4159
Size: 5260
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5260
PfoI T'CCnGG_A
Cuts at: 2224 2224
Size: 5260
PmeI GTTT'AAAC
Cuts at: 2564 2564
Size: 5260
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 4842 4874 3329
Size: 32 1481 32 3715
Fragments arranged by size:
3715 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5260
PsiI TTA'TAA
Cuts at: 2830 2830
Size: 5260
PstI C_TGCA'G
Cuts at: 830 2157 2612 830
Size: 1327 455 3478
Fragments arranged by size:
3478 1327 455
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5260
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5260
SalI G'TCGA_C
Cuts at: 1936 2602 1936
Size: 666 4594
SbfI CC_TGCA'GG
Cuts at: 2612 2612
Size: 5260
ScaI AGT'ACT
Cuts at: 3508 5220 3508
Size: 1712 3548
SfcI C'TryA_G
Cuts at: 826 1045 2153 2608 3743 4424 4615 826
Size: 219 1108 455 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 681 455 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5260
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5260
SmaI CCC'GGG
Cuts at: 1872 2587 1872
Size: 715 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3364 4265 4527 820
Size: 197 926 1421 901 262 1553
Fragments arranged by size:
1553 1421 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3051 493
Size: 2558 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5260
SphI G_CATG'C
Cuts at: 1132 2618 1132
Size: 1486 3774
SspI AAT'ATT
Cuts at: 5 52 3184 5
Size: 47 3132 2081
Fragments arranged by size:
3132 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5260
StyI C'CwwG_G
Cuts at: 513 1065 2407 2699 513
Size: 552 1342 292 3074
Fragments arranged by size:
3074 1342 552 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2198 3287 3304 3457 3642 4087 325
Size: 1255 618 1089 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1089 618 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3506 5218 96
Size: 274 80 33 51 1443 1529 1712 138
Fragments arranged by size:
1712 1529 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 5220 5252 2173
Size: 32 3015 32 2181
Fragments arranged by size:
3015 2181 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5260
XbaI T'CTAG_A
Cuts at: 2596 2596
Size: 5260
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5260
XmaI C'CCGG_G
Cuts at: 1870 2585 1870
Size: 715 4545
XmnI GAAnn'nnTTC
Cuts at: 3389 3389
Size: 5260
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3068 276
Size: 53 83 186 936 1534 2468
Fragments arranged by size:
2468 1534 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI