Restriction map for
pFN21AE6509
(Circular) (Six-base) MAPSORT of: pFN21AE6509.seq Check: 2545 from: 1 to: 5233
pFN21AE6509 5233 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2383 2383
Size: 5233
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3043 278
Size: 53 83 186 936 1507 2468
Fragments arranged by size:
2468 1507 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2576 1937
Size: 639 4594
Acc65I G'GTAC_C
Cuts at: 2554 2554
Size: 5233
AclI AA'CG_TT
Cuts at: 1563 3360 3733 1563
Size: 1797 373 3063
Fragments arranged by size:
3063 1797 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 3296 4680 4948 1632
Size: 409 1255 1384 268 1917
Fragments arranged by size:
1917 1384 1255 409 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5036
AflIII A'CryG_T
Cuts at: 1203 4132 5109 1203
Size: 2929 977 1327
Fragments arranged by size:
2929 1327 977
AgeI A'CCGG_T
Cuts at: 4064 4064
Size: 5233
AhdI GACnn_n'nnGTC
Cuts at: 2471 3962 2471
Size: 1491 3742
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5233
AlwNI CAG_nnn'CTG
Cuts at: 2976 4548 2976
Size: 1572 3661
ApaLI G'TGCA_C
Cuts at: 3290 4446 3290
Size: 1156 4077
ApoI r'AATT_y
Cuts at: 1493 2542 2768 2920 5029 1493
Size: 1049 226 152 2109 1697
Fragments arranged by size:
2109 1697 1049 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3787 160
Size: 872 2755 1606
Fragments arranged by size:
2755 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5233
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 2558 1870
Size: 73 227 388 4545
Fragments arranged by size:
4545 388 227 73
BamHI G'GATC_C
Cuts at: 2563 2930 2563
Size: 367 4866
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2554 4009 618
Size: 325 217 627 767 1455 1842
Fragments arranged by size:
1842 1455 767 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2421 2552 1391
Size: 1030 131 4072
Fragments arranged by size:
4072 1030 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3424 3458 1903
Size: 34 1487 34 3678
Fragments arranged by size:
3678 1487 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3125 4335 3125
Size: 1210 4023
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5233
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2156 2342 3844
Size: 107 122 71 631 605 484 186 1502
Cuts at: 3844 136
Size: 1525
Fragments arranged by size:
1525 1502 631 605 484 186 122 107
71
BglII A'GATC_T
Cuts at: 5228 5228
Size: 5233
BlpI GC'TnA_GC
Cuts at: 2650 2650
Size: 5233
Bme1580I G_kGCm'C
Cuts at: 1790 3294 4450 1790
Size: 1504 1156 2573
Fragments arranged by size:
2573 1504 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5233
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2964 3922 442
Size: 416 53 83 399 24 1547 958 1753
Fragments arranged by size:
1753 1547 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5233
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 5233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3893 1239
Size: 2654 2579
Bpu10I CC'TnA_GC
Cuts at: 2018 2018
Size: 5233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 3358 4223 4521 2414
Size: 944 865 298 3126
Fragments arranged by size:
3126 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3896 882
Size: 617 2397 2219
Fragments arranged by size:
2397 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3024 493
Size: 711 1820 2702
Fragments arranged by size:
2702 1820 711
BsaBI GATnn'nnATC
Cuts at: 5227 5227
Size: 5233
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3040 3422 4057 275
Size: 53 83 186 936 1507 382 635 1451
Fragments arranged by size:
1507 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2112 3666 4064 4338 4485 4896 2112
Size: 1554 398 274 147 411 2449
Fragments arranged by size:
2449 1554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5008
Fragments arranged by size:
5008 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2486 1186
Size: 1300 3933
BseYI C'CCAG_C
Cuts at: 2126 4436 4932 2126
Size: 2310 496 2427
Fragments arranged by size:
2427 2310 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4660
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2683 3444 4472 1540
Size: 275 186 682 761 1028 2301
Fragments arranged by size:
2301 1028 761 682 275 186
BsiHKAI G_wGCw'C
Cuts at: 2400 2421 2496 3294 3379 4450 2400
Size: 21 75 798 85 1071 3183
Fragments arranged by size:
3183 1071 798 85 75 21
BsmI GAATG_Cn'
Cuts at: 2744 2837 2744
Size: 93 5140
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2400 2421 2496 2552 3294 3379 4450
Size: 399 610 21 75 56 742 85 1071
Cuts at: 4450 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 610 399 85 75 56
21
BspEI T'CCGG_A
Cuts at: 4896 4896
Size: 5233
BspHI T'CATG_A
Cuts at: 3122 4852 3122
Size: 1730 3503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2383 2588 844
Size: 1539 205 3489
Fragments arranged by size:
3489 1539 205
BsrBI CCG'CTC
Cuts at: 1394 1823 3120 1394
Size: 429 1297 3507
Fragments arranged by size:
3507 1297 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2434 3728 3902 65
Size: 1183 1186 1294 174 1396
Fragments arranged by size:
1396 1294 1186 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2321 3877 4064 1700
Size: 110 511 1556 187 2869
Fragments arranged by size:
2869 1556 511 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5233
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5233
BssSI C'ACGA_G
Cuts at: 3293 4305 3293
Size: 1012 4221
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5233
BstBI TT'CG_AA
Cuts at: 4037 4037
Size: 5233
BstEII G'GTnAC_C
Cuts at: 1184 2473 1184
Size: 1289 3944
BstYI r'GATC_y
Cuts at: 1972 2563 2930 3315 3332 4784 4870 4882 5228
Size: 591 367 385 17 1452 86 12 346
Cuts at: 5228 1972
Size: 1977
Fragments arranged by size:
1977 1452 591 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4681
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4494
Fragments arranged by size:
4494 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2749 3543 3563 2130
Size: 619 794 20 3800
Fragments arranged by size:
3800 794 619 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5233
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5198
DraI TTT'AAA
Cuts at: 2537 2537
Size: 5233
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4240 809
Size: 507 2924 1802
Fragments arranged by size:
2924 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2680 3569
Size: 54 1425 50 275 18 204 646 889
Cuts at: 3569 5198 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 646 275 204 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2680 1537
Size: 275 868 4090
Fragments arranged by size:
4090 868 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 3163 1353
Size: 707 1103 3423
Fragments arranged by size:
3423 1103 707
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3820 4194 4340 387
Size: 1064 138 2231 374 146 1280
Fragments arranged by size:
2231 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2419 2419
Size: 5233
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 3296 3893 4680 4948 1239
Size: 393 409 1255 597 787 268 1524
Fragments arranged by size:
1524 1255 787 597 409 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2119 2119
Size: 5233
EcoRI G'AATT_C
Cuts at: 2542 2920 2542
Size: 378 4855
FspI TGC'GCA
Cuts at: 3739 3739
Size: 5233
HaeII r_GCGC'y
Cuts at: 1678 4380 1678
Size: 2702 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2166 2198 2457 2489 3874
Size: 32 135 32 594 32 259 32 1385
Cuts at: 3874 3906 3948 3980 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1385 594 259 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2577 2823 669
Size: 988 281 639 246 3079
Fragments arranged by size:
3079 988 639 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5233
HpaI GTT'AAC
Cuts at: 2823 2823
Size: 5233
KpnI G_GTAC'C
Cuts at: 2558 2558
Size: 5233
MfeI C'AATT_G
Cuts at: 2832 2832
Size: 5233
MluI A'CGCG_T
Cuts at: 5109 5109
Size: 5233
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2430 4347 4531 1305
Size: 1125 1917 184 2007
Fragments arranged by size:
2007 1917 1125 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2036 10
Size: 54 1425 343 204 3207
Fragments arranged by size:
3207 1425 343 204 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3191 3550 3709 518
Size: 648 2025 359 159 2042
Fragments arranged by size:
2042 2025 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2649 3326 4474 4719 1606
Size: 1043 677 1148 245 2120
Fragments arranged by size:
2120 1148 1043 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4681
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5233
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5233
NotI GC'GGCC_GC
Cuts at: 2680 2680
Size: 5233
NspI r_CATG'y
Cuts at: 1132 1229 2050 2591 4136 1132
Size: 97 821 541 1545 2229
Fragments arranged by size:
2229 1545 821 541 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5233
PciI A'CATG_T
Cuts at: 4132 4132
Size: 5233
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2250 1108
Size: 1142 4091
PfoI T'CCnGG_A
Cuts at: 2412 2412
Size: 5233
PmeI GTTT'AAAC
Cuts at: 2537 2537
Size: 5233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3302 3334 4815 4847 3302
Size: 32 1481 32 3688
Fragments arranged by size:
3688 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5233
PsiI TTA'TAA
Cuts at: 2803 2803
Size: 5233
PstI C_TGCA'G
Cuts at: 830 2585 830
Size: 1755 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5233
SacI G_AGCT'C
Cuts at: 2421 2421
Size: 5233
SalI G'TCGA_C
Cuts at: 1936 2575 1936
Size: 639 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2060
Size: 5233
SbfI CC_TGCA'GG
Cuts at: 2585 2585
Size: 5233
ScaI AGT'ACT
Cuts at: 3481 5193 3481
Size: 1712 3521
SfcI C'TryA_G
Cuts at: 826 1045 2581 3716 4397 4588 826
Size: 219 1536 1135 681 191 1471
Fragments arranged by size:
1536 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5233
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5233
SmaI CCC'GGG
Cuts at: 1872 2172 2560 1872
Size: 300 388 4545
Fragments arranged by size:
4545 388 300
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 3337 4238 4500 820
Size: 197 926 486 908 901 262 1553
Fragments arranged by size:
1553 926 908 901 486 262 197
SnaBI TAC'GTA
Cuts at: 493 3024 493
Size: 2531 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5233
SphI G_CATG'C
Cuts at: 1132 2050 2591 1132
Size: 918 541 3774
Fragments arranged by size:
3774 918 541
SspI AAT'ATT
Cuts at: 5 52 3157 5
Size: 47 3105 2081
Fragments arranged by size:
3105 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5233
StyI C'CwwG_G
Cuts at: 513 1065 2304 2672 513
Size: 552 1239 368 3074
Fragments arranged by size:
3074 1239 552 368
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 3260 3277 3430 3615 4060 325
Size: 1255 774 906 17 153 185 445 1498
Fragments arranged by size:
1498 1255 906 774 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3479 5191 96
Size: 274 80 33 51 1443 1502 1712 138
Fragments arranged by size:
1712 1502 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5193 5225 5193
Size: 32 5201
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5233
XbaI T'CTAG_A
Cuts at: 2569 2569
Size: 5233
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5233
XmaI C'CCGG_G
Cuts at: 1870 2170 2558 1870
Size: 300 388 4545
Fragments arranged by size:
4545 388 300
XmnI GAAnn'nnTTC
Cuts at: 3362 3362
Size: 5233
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3041 276
Size: 53 83 186 936 1507 2468
Fragments arranged by size:
2468 1507 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI