Restriction map for
pFN21AE6508
(Circular) (Six-base) MAPSORT of: pFN21AE6508.seq Check: 4494 from: 1 to: 5230
pFN21AE6508 5230 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3040 278
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2573 1937
Size: 636 4594
Acc65I G'GTAC_C
Cuts at: 2551 2551
Size: 5230
AclI AA'CG_TT
Cuts at: 1563 3357 3730 1563
Size: 1794 373 3063
Fragments arranged by size:
3063 1794 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2371 3293 4677 4945 1632
Size: 739 922 1384 268 1917
Fragments arranged by size:
1917 1384 922 739 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5033
AflIII A'CryG_T
Cuts at: 1203 4129 5106 1203
Size: 2926 977 1327
Fragments arranged by size:
2926 1327 977
AgeI A'CCGG_T
Cuts at: 4061 4061
Size: 5230
AhdI GACnn_n'nnGTC
Cuts at: 3959 3959
Size: 5230
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5230
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2279 1184
Size: 1095 4135
AlwNI CAG_nnn'CTG
Cuts at: 2066 2973 4545 2066
Size: 907 1572 2751
Fragments arranged by size:
2751 1572 907
ApaLI G'TGCA_C
Cuts at: 3287 4443 3287
Size: 1156 4074
ApoI r'AATT_y
Cuts at: 1493 2539 2765 2917 5026 1493
Size: 1046 226 152 2109 1697
Fragments arranged by size:
2109 1697 1046 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3784 160
Size: 872 2752 1606
Fragments arranged by size:
2752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
AvaI C'yCGr_G
Cuts at: 1870 1943 2555 1870
Size: 73 612 4545
Fragments arranged by size:
4545 612 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2502 2535 2502
Size: 33 5197
BamHI G'GATC_C
Cuts at: 2560 2927 2560
Size: 367 4863
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2551 4006 618
Size: 325 217 627 764 1455 1842
Fragments arranged by size:
1842 1455 764 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2549 1391
Size: 1158 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3421 3455 1903
Size: 34 1484 34 3678
Fragments arranged by size:
3678 1484 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2530 3122 4332 2530
Size: 592 1210 3428
Fragments arranged by size:
3428 1210 592
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5230
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3841 136
Size: 107 122 71 631 605 337 1832 1525
Fragments arranged by size:
1832 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5225 5225
Size: 5230
BlpI GC'TnA_GC
Cuts at: 2647 2647
Size: 5230
Bme1580I G_kGCm'C
Cuts at: 1790 3291 4447 1790
Size: 1501 1156 2573
Fragments arranged by size:
2573 1501 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2961 3919 442
Size: 416 53 83 399 24 1544 958 1753
Fragments arranged by size:
1753 1544 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2292 3890 1239
Size: 1053 1598 2579
Fragments arranged by size:
2579 1598 1053
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2130 3355 4220 4518 2130
Size: 1225 865 298 2842
Fragments arranged by size:
2842 1225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2020 3893 882
Size: 617 521 1873 2219
Fragments arranged by size:
2219 1873 617 521
BsaAI yAC'GTr
Cuts at: 493 1204 3021 493
Size: 711 1817 2702
Fragments arranged by size:
2702 1817 711
BsaBI GATnn'nnATC
Cuts at: 5224 5224
Size: 5230
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3037 3419 4054 275
Size: 53 83 186 936 1504 382 635 1451
Fragments arranged by size:
1504 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3663 4061 4335 4482 4893 3663
Size: 398 274 147 411 4000
Fragments arranged by size:
4000 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5005
Fragments arranged by size:
5005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5230
BseYI C'CCAG_C
Cuts at: 4433 4929 4433
Size: 496 4734
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4657
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2680 3441 4469 1540
Size: 275 186 679 761 1028 2301
Fragments arranged by size:
2301 1028 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 3291 3376 4447 3291
Size: 85 1071 4074
Fragments arranged by size:
4074 1071 85
BsmI GAATG_Cn'
Cuts at: 2741 2834 2741
Size: 93 5137
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2549 3291 3376 4447 1391
Size: 399 759 742 85 1071 2174
Fragments arranged by size:
2174 1071 759 742 399 85
BspEI T'CCGG_A
Cuts at: 4893 4893
Size: 5230
BspHI T'CATG_A
Cuts at: 3119 4849 3119
Size: 1730 3500
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2585 844
Size: 1741 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3117 1394
Size: 429 1294 3507
Fragments arranged by size:
3507 1294 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3725 3899 65
Size: 1183 2477 174 1396
Fragments arranged by size:
2477 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3874 4061 1700
Size: 110 2064 187 2869
Fragments arranged by size:
2869 2064 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5230
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5230
BssSI C'ACGA_G
Cuts at: 3290 4302 3290
Size: 1012 4218
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5230
BstBI TT'CG_AA
Cuts at: 4034 4034
Size: 5230
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5230
BstYI r'GATC_y
Cuts at: 1972 2238 2560 2927 3312 3329 4781 4867 4879
Size: 266 322 367 385 17 1452 86 12
Cuts at: 4879 5225 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 385 367 346 322 266 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2335 487
Size: 683 56 1109 3382
Fragments arranged by size:
3382 1109 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2746 3540 3560 2067
Size: 679 794 20 3737
Fragments arranged by size:
3737 794 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2007 2042 529
Size: 35 1443 35 3717
Fragments arranged by size:
3717 1443 35 35
DraI TTT'AAA
Cuts at: 2534 2534
Size: 5230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4237 809
Size: 507 2921 1802
Fragments arranged by size:
2921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2677 3566 5195
Size: 54 1425 50 275 18 847 889 1629
Cuts at: 5195 8
Size: 43
Fragments arranged by size:
1629 1425 889 847 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2677 1537
Size: 275 865 4090
Fragments arranged by size:
4090 865 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2205 2442 3160 1353
Size: 852 237 718 3423
Fragments arranged by size:
3423 852 718 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2065 3817 4191 4337 387
Size: 1064 138 476 1752 374 146 1280
Fragments arranged by size:
1752 1280 1064 476 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2292 2371 3293 3890 4677 4945 1239
Size: 393 660 79 922 597 787 268 1524
Fragments arranged by size:
1524 922 787 660 597 393 268 79
EcoRI G'AATT_C
Cuts at: 2539 2917 2539
Size: 378 4852
EcoRV GAT'ATC
Cuts at: 2185 2185
Size: 5230
FspI TGC'GCA
Cuts at: 3736 3736
Size: 5230
HaeII r_GCGC'y
Cuts at: 1678 4377 1678
Size: 2699 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 3871 3903 3945
Size: 32 135 32 779 32 1488 32 42
Cuts at: 3945 3977 1373
Size: 32 2626
Fragments arranged by size:
2626 1488 779 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2574 2820 669
Size: 988 281 636 246 3079
Fragments arranged by size:
3079 988 636 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5230
HpaI GTT'AAC
Cuts at: 2820 2820
Size: 5230
KpnI G_GTAC'C
Cuts at: 2555 2555
Size: 5230
MfeI C'AATT_G
Cuts at: 2829 2829
Size: 5230
MluI A'CGCG_T
Cuts at: 5106 5106
Size: 5230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2120 2394 4344 4528 1305
Size: 815 274 1950 184 2007
Fragments arranged by size:
2007 1950 815 274 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3408
Fragments arranged by size:
3408 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3188 3547 3706 518
Size: 648 2022 359 159 2042
Fragments arranged by size:
2042 2022 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2646 3323 4471 4716 1606
Size: 1040 677 1148 245 2120
Fragments arranged by size:
2120 1148 1040 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3739
Fragments arranged by size:
3739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5230
NotI GC'GGCC_GC
Cuts at: 2677 2677
Size: 5230
NspI r_CATG'y
Cuts at: 1132 1229 2266 2588 4133 1132
Size: 97 1037 322 1545 2229
Fragments arranged by size:
2229 1545 1037 322 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5230
PciI A'CATG_T
Cuts at: 4129 4129
Size: 5230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5230
PmeI GTTT'AAAC
Cuts at: 2534 2534
Size: 5230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 4812 4844 3299
Size: 32 1481 32 3685
Fragments arranged by size:
3685 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5230
PsiI TTA'TAA
Cuts at: 2800 2800
Size: 5230
PstI C_TGCA'G
Cuts at: 830 2582 830
Size: 1752 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5230
SalI G'TCGA_C
Cuts at: 1936 2572 1936
Size: 636 4594
SapI GCTCTTCn'nnn_
Cuts at: 2205 2205
Size: 5230
SbfI CC_TGCA'GG
Cuts at: 2582 2582
Size: 5230
ScaI AGT'ACT
Cuts at: 2392 3478 5190 2392
Size: 1086 1712 2432
Fragments arranged by size:
2432 1712 1086
SexAI A'CCwGG_T
Cuts at: 2099 2099
Size: 5230
SfcI C'TryA_G
Cuts at: 826 1045 2067 2578 3713 4394 4585 826
Size: 219 1022 511 1135 681 191 1471
Fragments arranged by size:
1471 1135 1022 681 511 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5230
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5230
SmaI CCC'GGG
Cuts at: 1872 2557 1872
Size: 685 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2145 3334 4235 4497 820
Size: 197 926 202 1189 901 262 1553
Fragments arranged by size:
1553 1189 926 901 262 202 197
SnaBI TAC'GTA
Cuts at: 493 3021 493
Size: 2528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5230
SphI G_CATG'C
Cuts at: 1132 2588 1132
Size: 1456 3774
SspI AAT'ATT
Cuts at: 5 52 3154 5
Size: 47 3102 2081
Fragments arranged by size:
3102 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5230
StyI C'CwwG_G
Cuts at: 513 1065 2004 2669 513
Size: 552 939 665 3074
Fragments arranged by size:
3074 939 665 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3274 3427 3612 4057 325
Size: 1255 1677 17 153 185 445 1498
Fragments arranged by size:
1677 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2390 3476 5188
Size: 274 80 33 51 1443 413 1086 1712
Cuts at: 5188 96
Size: 138
Fragments arranged by size:
1712 1443 1086 413 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5190 5222 5190
Size: 32 5198
Tth111I GACn'n_nGTC
Cuts at: 1316 2101 1316
Size: 785 4445
XbaI T'CTAG_A
Cuts at: 2566 2566
Size: 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5230
XmaI C'CCGG_G
Cuts at: 1870 2555 1870
Size: 685 4545
XmnI GAAnn'nnTTC
Cuts at: 3359 3359
Size: 5230
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3038 276
Size: 53 83 186 936 1504 2468
Fragments arranged by size:
2468 1504 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SfiI SfoI SrfI SwaI XcmI