Restriction map for
pFN21AE6507
(Circular) (Six-base) MAPSORT of: pFN21AE6507.seq Check: 2342 from: 1 to: 5335
pFN21AE6507 5335 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3145 278
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 1937
Size: 741 4594
Acc65I G'GTAC_C
Cuts at: 2656 2656
Size: 5335
AclI AA'CG_TT
Cuts at: 1563 3462 3835 1563
Size: 1899 373 3063
Fragments arranged by size:
3063 1899 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3398 4782 5050 1632
Size: 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5138
AflIII A'CryG_T
Cuts at: 1203 2358 4234 5211 1203
Size: 1155 1876 977 1327
Fragments arranged by size:
1876 1327 1155 977
AgeI A'CCGG_T
Cuts at: 4166 4166
Size: 5335
AhdI GACnn_n'nnGTC
Cuts at: 2542 4064 2542
Size: 1522 3813
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5335
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5335
AlwNI CAG_nnn'CTG
Cuts at: 3078 4650 3078
Size: 1572 3763
ApaLI G'TGCA_C
Cuts at: 3392 4548 3392
Size: 1156 4179
ApoI r'AATT_y
Cuts at: 1493 2233 2644 2870 3022 5131 1493
Size: 740 411 226 152 2109 1697
Fragments arranged by size:
2109 1697 740 411 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3889 160
Size: 872 2857 1606
Fragments arranged by size:
2857 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2079 2660 1870
Size: 73 72 64 581 4545
Fragments arranged by size:
4545 581 73 72 64
BamHI G'GATC_C
Cuts at: 2665 3032 2665
Size: 367 4968
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2656 4111 618
Size: 325 217 627 232 637 1455 1842
Fragments arranged by size:
1842 1455 637 627 325 232 217
BanII G_rGCy'C
Cuts at: 1391 2574 2654 1391
Size: 1183 80 4072
Fragments arranged by size:
4072 1183 80
BbeI G_GCGC'C
Cuts at: 2023 2023
Size: 5335
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2343 2598 928
Size: 971 444 255 3665
Fragments arranged by size:
3665 971 444 255
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2122 2126 2156 2160 3526 3560 1903
Size: 34 185 4 30 4 1366 34 3678
Fragments arranged by size:
3678 1366 185 34 34 30 4 4
BciVI GTATCCnnnnn_n'
Cuts at: 3227 4437 3227
Size: 1210 4125
BclI T'GATC_A
Cuts at: 1548 2436 1548
Size: 888 4447
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2124 2265 3946
Size: 107 122 71 631 605 452 141 1681
Cuts at: 3946 136
Size: 1525
Fragments arranged by size:
1681 1525 631 605 452 141 122 107
71
BglII A'GATC_T
Cuts at: 2419 5330 2419
Size: 2911 2424
BlpI GC'TnA_GC
Cuts at: 2752 2752
Size: 5335
Bme1580I G_kGCm'C
Cuts at: 1790 3396 4552 1790
Size: 1606 1156 2573
Fragments arranged by size:
2573 1606 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5335
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2420 2520 2530
Size: 416 53 83 399 24 1003 100 10
Cuts at: 2530 3066 4024 442
Size: 536 958 1753
Fragments arranged by size:
1753 1003 958 536 416 399 100 83
53 24 10
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3995 1239
Size: 2756 2579
Bpu10I CC'TnA_GC
Cuts at: 2493 2493
Size: 5335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3460 4325 4623 3460
Size: 865 298 4172
Fragments arranged by size:
4172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 3998 882
Size: 617 774 1725 2219
Fragments arranged by size:
2219 1725 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 3126 493
Size: 711 1922 2702
Fragments arranged by size:
2702 1922 711
BsaBI GATnn'nnATC
Cuts at: 5329 5329
Size: 5335
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2020 2045 2450 3142
Size: 53 83 186 936 487 25 405 692
Cuts at: 3142 3524 4159 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 692 635 487 405 382 186
83 53 25
BsaWI w'CCGG_w
Cuts at: 2621 3768 4166 4440 4587 4998 2621
Size: 1147 398 274 147 411 2958
Fragments arranged by size:
2958 1147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5110
Fragments arranged by size:
5110 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5335
BseYI C'CCAG_C
Cuts at: 2051 4538 5034 2051
Size: 2487 496 2352
Fragments arranged by size:
2487 2352 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2137 1163
Size: 573 401 4361
Fragments arranged by size:
4361 573 401
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2093 2119 2785 3546 4574 1540
Size: 275 186 92 26 666 761 1028 2301
Fragments arranged by size:
2301 1028 761 666 275 186 92 26
BsiHKAI G_wGCw'C
Cuts at: 2574 3396 3481 4552 2574
Size: 822 85 1071 3357
Fragments arranged by size:
3357 1071 822 85
BsmI GAATG_Cn'
Cuts at: 2846 2939 2846
Size: 93 5242
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2574 2654 3396 3481 4552 1391
Size: 399 784 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 784 742 399 85 80
BspEI T'CCGG_A
Cuts at: 2621 4998 2621
Size: 2377 2958
BspHI T'CATG_A
Cuts at: 3224 4954 3224
Size: 1730 3605
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2159 2690 844
Size: 1315 531 3489
Fragments arranged by size:
3489 1315 531
BsrBI CCG'CTC
Cuts at: 1394 1823 3222 1394
Size: 429 1399 3507
Fragments arranged by size:
3507 1399 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3830 4004 65
Size: 1183 2582 174 1396
Fragments arranged by size:
2582 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2092 3979 4166 1700
Size: 110 223 59 1887 187 2869
Fragments arranged by size:
2869 1887 223 187 110 59
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5335
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5335
BssSI C'ACGA_G
Cuts at: 3395 4407 3395
Size: 1012 4323
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5335
BstBI TT'CG_AA
Cuts at: 2174 4139 2174
Size: 1965 3370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5335
BstYI r'GATC_y
Cuts at: 1972 2419 2665 3032 3417 3434 4886 4972 4984
Size: 447 246 367 385 17 1452 86 12
Cuts at: 4984 5330 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 447 385 367 346 246 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2087 2122 513
Size: 552 1022 35 3726
Fragments arranged by size:
3726 1022 552 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4596
Fragments arranged by size:
4596 683 56
BtsI GCAGTG_nn'
Cuts at: 2851 3645 3665 2851
Size: 794 20 4521
Fragments arranged by size:
4521 794 20
ClaI AT'CG_AT
Cuts at: 1554 2142 1554
Size: 588 4747
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2395 2430 529
Size: 35 1831 35 3434
Fragments arranged by size:
3434 1831 35 35
DraI TTT'AAA
Cuts at: 2639 2639
Size: 5335
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2375 4342 809
Size: 507 1059 1967 1802
Fragments arranged by size:
1967 1802 1059 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 2116 2562
Size: 54 1425 50 275 18 260 26 446
Cuts at: 2562 2782 3671 5300 8
Size: 220 889 1629 43
Fragments arranged by size:
1629 1425 889 446 275 260 220 54
50 43 26 18
EagI C'GGCC_G
Cuts at: 1537 1812 2090 2116 2782 1537
Size: 275 278 26 666 4090
Fragments arranged by size:
4090 666 278 275 26
EarI CTCTTCn'nnn_
Cuts at: 1353 3265 1353
Size: 1912 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3922 4296 4442 387
Size: 1064 138 2333 374 146 1280
Fragments arranged by size:
2333 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2572 2572
Size: 5335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3398 3995 4782 5050 1239
Size: 393 1766 597 787 268 1524
Fragments arranged by size:
1766 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 5335
EcoRI G'AATT_C
Cuts at: 2644 3022 2644
Size: 378 4957
FspI TGC'GCA
Cuts at: 2132 3841 2132
Size: 1709 3626
HaeII r_GCGC'y
Cuts at: 1678 2023 4482 1678
Size: 345 2459 2531
Fragments arranged by size:
2531 2459 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 3976 4008 4050
Size: 32 135 32 956 32 1416 32 42
Cuts at: 4050 4082 1373
Size: 32 2626
Fragments arranged by size:
2626 1416 956 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 2925 669
Size: 988 281 741 246 3079
Fragments arranged by size:
3079 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5335
HpaI GTT'AAC
Cuts at: 2925 2925
Size: 5335
KasI G'GCGC_C
Cuts at: 2019 2019
Size: 5335
KpnI G_GTAC'C
Cuts at: 2660 2660
Size: 5335
MfeI C'AATT_G
Cuts at: 2934 2934
Size: 5335
MluI A'CGCG_T
Cuts at: 5211 5211
Size: 5335
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4449 4633 1305
Size: 3144 184 2007
Fragments arranged by size:
3144 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3513
Fragments arranged by size:
3513 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3293 3652 3811 518
Size: 648 2127 359 159 2042
Fragments arranged by size:
2127 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2089 2751 3428 4576 4821 1606
Size: 483 662 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 483 245
NaeI GCC'GGC
Cuts at: 2035 2094 2035
Size: 59 5276
NarI GG'CG_CC
Cuts at: 2020 2020
Size: 5335
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4783
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5335
NgoMIV G'CCGG_C
Cuts at: 2033 2092 2033
Size: 59 5276
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5335
NotI GC'GGCC_GC
Cuts at: 2116 2782 2116
Size: 666 4669
NspI r_CATG'y
Cuts at: 1132 1229 2693 4238 1132
Size: 97 1464 1545 2229
Fragments arranged by size:
2229 1545 1464 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5335
PciI A'CATG_T
Cuts at: 4234 4234
Size: 5335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2486 1108
Size: 1378 3957
PmeI GTTT'AAAC
Cuts at: 2639 2639
Size: 5335
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3404 3436 4917 4949 3404
Size: 32 1481 32 3790
Fragments arranged by size:
3790 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 5335
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5335
PsiI TTA'TAA
Cuts at: 2905 2905
Size: 5335
PstI C_TGCA'G
Cuts at: 830 2687 830
Size: 1857 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5335
SacI G_AGCT'C
Cuts at: 2574 2574
Size: 5335
SacII CC_GC'GG
Cuts at: 2090 2090
Size: 5335
SalI G'TCGA_C
Cuts at: 1936 2677 1936
Size: 741 4594
SanDI GG'GwC_CC
Cuts at: 2083 2083
Size: 5335
SbfI CC_TGCA'GG
Cuts at: 2687 2687
Size: 5335
ScaI AGT'ACT
Cuts at: 3583 5295 3583
Size: 1712 3623
SfcI C'TryA_G
Cuts at: 826 1045 2683 3818 4499 4690 826
Size: 219 1638 1135 681 191 1471
Fragments arranged by size:
1638 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2124 2124
Size: 5335
SfoI GGC'GCC
Cuts at: 2021 2021
Size: 5335
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5335
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5335
SmaI CCC'GGG
Cuts at: 1872 2081 2662 1872
Size: 209 581 4545
Fragments arranged by size:
4545 581 209
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3439 4340 4602 820
Size: 197 926 1496 901 262 1553
Fragments arranged by size:
1553 1496 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3126 493
Size: 2633 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5335
SphI G_CATG'C
Cuts at: 1132 2693 1132
Size: 1561 3774
SspI AAT'ATT
Cuts at: 5 52 3259 5
Size: 47 3207 2081
Fragments arranged by size:
3207 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5335
StyI C'CwwG_G
Cuts at: 513 1065 2610 2774 513
Size: 552 1545 164 3074
Fragments arranged by size:
3074 1545 552 164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2065 2277 2402 2512 3362 3379 3532
Size: 1255 485 212 125 110 850 17 153
Cuts at: 3532 3717 4162 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 850 485 445 212 185 153
125 110 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2526 3581 5293
Size: 274 80 33 51 1443 549 1055 1712
Cuts at: 5293 96
Size: 138
Fragments arranged by size:
1712 1443 1055 549 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2418 2450 5295 5327 2418
Size: 32 2845 32 2426
Fragments arranged by size:
2845 2426 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5335
XbaI T'CTAG_A
Cuts at: 2272 2671 2272
Size: 399 4936
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5335
XmaI C'CCGG_G
Cuts at: 1870 2079 2660 1870
Size: 209 581 4545
Fragments arranged by size:
4545 581 209
XmnI GAAnn'nnTTC
Cuts at: 3464 3464
Size: 5335
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3143 276
Size: 53 83 186 936 1609 2468
Fragments arranged by size:
2468 1609 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SapI
SexAI SrfI SwaI XcmI