Restriction map for
pFN21AE6496
(Circular) (Six-base) MAPSORT of: pFN21AE6496.seq Check: 9217 from: 1 to: 6301
pFN21AE6496 6301 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3075 3075
Size: 6301
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4111 278
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3030 3195 3264 3644 1937
Size: 1093 165 69 380 4594
Fragments arranged by size:
4594 1093 380 165 69
Acc65I G'GTAC_C
Cuts at: 3622 3622
Size: 6301
AclI AA'CG_TT
Cuts at: 1563 4428 4801 1563
Size: 2865 373 3063
Fragments arranged by size:
3063 2865 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4364 5748 6016 1632
Size: 2732 1384 268 1917
Fragments arranged by size:
2732 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 2583 5200 6177 1203
Size: 1380 2617 977 1327
Fragments arranged by size:
2617 1380 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2747 1184
Size: 1563 4738
AlwNI CAG_nnn'CTG
Cuts at: 2563 3076 4044 5616 2563
Size: 513 968 1572 3248
Fragments arranged by size:
3248 1572 968 513
ApaI G_GGCC'C
Cuts at: 2216 2436 3312 2216
Size: 220 876 5205
Fragments arranged by size:
5205 876 220
ApaLI G'TGCA_C
Cuts at: 2640 3332 4358 5514 2640
Size: 692 1026 1156 3427
Fragments arranged by size:
3427 1156 1026 692
ApoI r'AATT_y
Cuts at: 1493 3610 3836 3988 6097 1493
Size: 2117 226 152 2109 1697
Fragments arranged by size:
2117 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4855 160
Size: 872 3823 1606
Fragments arranged by size:
3823 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2228 2820 3305 3626 1870
Size: 73 91 194 592 485 321 4545
Fragments arranged by size:
4545 592 485 321 194 91 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2100 3107 3622 5077 618
Size: 325 217 627 313 1007 515 1455 1842
Fragments arranged by size:
1842 1455 1007 627 515 325 313 217
BanII G_rGCy'C
Cuts at: 1391 2088 2216 2244 2330 2436 2570 2821 3312
Size: 697 128 28 86 106 134 251 491
Cuts at: 3312 3620 1391
Size: 308 4072
Fragments arranged by size:
4072 697 491 308 251 134 128 106
86 28
BbeI G_GCGC'C
Cuts at: 2104 3111 2104
Size: 1007 5294
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 928
Size: 971 471 4859
Fragments arranged by size:
4859 971 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4492 4526 1903
Size: 34 2555 34 3678
Fragments arranged by size:
3678 2555 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4193 5403 4193
Size: 1210 5091
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 2092 2285
Size: 107 122 71 631 605 367 53 193
Cuts at: 2285 2559 2885 3528 3549 4912 136
Size: 274 326 643 21 1363 1525
Fragments arranged by size:
1525 1363 643 631 605 367 326 274
193 122 107 71 53 21
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 2329 2569 3356 3718 2329
Size: 240 787 362 4912
Fragments arranged by size:
4912 787 362 240
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2178 2216 2436 2644 2736 2843 3129
Size: 379 9 38 220 208 92 107 286
Cuts at: 3129 3312 3336 4362 5518 1790
Size: 183 24 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 379 286 220 208 183
107 92 38 24 9
BmgBI CAC'GTC
Cuts at: 1318 2368 2461 2586 3235 1318
Size: 1050 93 125 649 4384
Fragments arranged by size:
4384 1050 649 125 93
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2654 4032 4990
Size: 416 53 83 399 24 1237 1378 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
1753 1378 1237 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2750 1056
Size: 1694 4607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4961 1239
Size: 3722 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2446 4426 5291 5589 2446
Size: 1980 865 298 3158
Fragments arranged by size:
3158 1980 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3163 4964 882
Size: 617 1664 1801 2219
Fragments arranged by size:
2219 1801 1664 617
BsaAI yAC'GTr
Cuts at: 493 1204 2946 4092 493
Size: 711 1742 1146 2702
Fragments arranged by size:
2702 1742 1146 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2101 2300 2484 3027
Size: 53 83 186 936 568 199 184 543
Cuts at: 3027 3108 4108 4490 5125 275
Size: 81 1000 382 635 1451
Fragments arranged by size:
1451 1000 936 635 568 543 382 199
186 184 83 81 53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2209 2239 1178
Size: 30 165 30 806 30 5240
Fragments arranged by size:
5240 806 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2636 1186
Size: 1450 4851
BseYI C'CCAG_C
Cuts at: 3022 3340 5504 6000 3022
Size: 318 2164 496 3323
Fragments arranged by size:
3323 2164 496 318
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2796 1163
Size: 573 1060 4668
Fragments arranged by size:
4668 1060 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2446 3751 4512 5540 1540
Size: 275 186 445 1305 761 1028 2301
Fragments arranged by size:
2301 1305 1028 761 445 275 186
BsiHKAI G_wGCw'C
Cuts at: 2644 3336 4362 4447 5518 2644
Size: 692 1026 85 1071 3427
Fragments arranged by size:
3427 1071 1026 692 85
BsmI GAATG_Cn'
Cuts at: 3812 3905 3812
Size: 93 6208
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2169 2178 2216 2244 2330 2436
Size: 399 298 81 9 38 28 86 106
Cuts at: 2436 2570 2644 2736 2821 2843 3129 3312 3336
Size: 134 74 92 85 22 286 183 24
Cuts at: 3336 3620 4362 4447 5518 1391
Size: 284 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 298 286 284 183
134 106 92 86 85 85 81 74
38 28 24 22 9
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2029 2838 3075 3390 3656 844
Size: 1185 809 237 315 266 3489
Fragments arranged by size:
3489 1185 809 315 266 237
BsrBI CCG'CTC
Cuts at: 1394 1823 2430 3138 4188 1394
Size: 429 607 708 1050 3507
Fragments arranged by size:
3507 1050 708 607 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2874 2937 4796 4970 65
Size: 1183 1626 63 1859 174 1396
Fragments arranged by size:
1859 1626 1396 1183 174 63
BsrFI r'CCGG_y
Cuts at: 1700 1810 2720 2888 3001 4945 5132 1700
Size: 110 910 168 113 1944 187 2869
Fragments arranged by size:
2869 1944 910 187 168 113 110
BsrGI T'GTAC_A
Cuts at: 96 2151 96
Size: 2055 4246
BssHII G'CGCG_C
Cuts at: 1925 2546 2892 3053 3084 1925
Size: 621 346 161 31 5142
Fragments arranged by size:
5142 621 346 161 31
BssSI C'ACGA_G
Cuts at: 3115 4361 5373 3115
Size: 1246 1012 4043
Fragments arranged by size:
4043 1246 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 3631 3998 4383 4400 5852 5938 5950 6296
Size: 1659 367 385 17 1452 86 12 346
Cuts at: 6296 1972
Size: 1977
Fragments arranged by size:
1977 1659 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 2880 513
Size: 552 939 9 867 3934
Fragments arranged by size:
3934 939 867 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2170 3256 3465 487
Size: 683 56 944 1086 209 3323
Fragments arranged by size:
3323 1086 944 683 209 56
BtsI GCAGTG_nn'
Cuts at: 2793 3077 3203 3817 4611 4631 2793
Size: 284 126 614 794 20 4463
Fragments arranged by size:
4463 794 614 284 126 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6266
DraI TTT'AAA
Cuts at: 3605 3605
Size: 6301
DraIII CAC_nnn'GTG
Cuts at: 3331 3331
Size: 6301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2265 2286
Size: 54 1425 50 275 18 213 222 21
Cuts at: 2286 2318 2790 2886 3375 3520 3748 4637 6266
Size: 32 472 96 489 145 228 889 1629
Cuts at: 6266 8
Size: 43
Fragments arranged by size:
1629 1425 889 489 472 275 228 222
213 145 96 54 50 43 32 21
18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4231 1353
Size: 2878 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4364 4961 5748 6016 1239
Size: 393 2732 597 787 268 1524
Fragments arranged by size:
2732 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2212 2213 2433 2560 2212
Size: 1 220 127 5953
Fragments arranged by size:
5953 220 127 1
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 2078 2104 2132 2280 2657 3111 5448 1678
Size: 400 26 28 148 377 454 2337 2531
Fragments arranged by size:
2531 2337 454 400 377 148 28 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4942 4974 5016 5048 1373
Size: 32 135 32 3370 32 42 32 2626
Fragments arranged by size:
3370 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3196 3645 3891 669
Size: 988 281 1258 449 246 3079
Fragments arranged by size:
3079 1258 988 449 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KasI G'GCGC_C
Cuts at: 2100 3107 2100
Size: 1007 5294
KpnI G_GTAC'C
Cuts at: 3626 3626
Size: 6301
MfeI C'AATT_G
Cuts at: 3900 3900
Size: 6301
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2037 5415 5599 1305
Size: 732 3378 184 2007
Fragments arranged by size:
3378 2007 732 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2320 2792 3377 3522 10
Size: 54 1425 343 488 472 585 145 2789
Fragments arranged by size:
2789 1425 585 488 472 343 145 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2597 2879 2948 4259 4618 4777 518
Size: 648 1431 282 69 1311 359 159 2042
Fragments arranged by size:
2042 1431 1311 648 359 282 159 69
MspA1I CmG'CkG
Cuts at: 1606 2015 2254 2312 2324 2410 2467 3717 4394
Size: 409 239 58 12 86 57 1250 677
Cuts at: 4394 5542 5787 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1250 1148 677 409 245 239 86
58 57 12
NaeI GCC'GGC
Cuts at: 2722 2890 3003 2722
Size: 168 113 6020
Fragments arranged by size:
6020 168 113
NarI GG'CG_CC
Cuts at: 2101 3108 2101
Size: 1007 5294
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4810
Fragments arranged by size:
4810 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NgoMIV G'CCGG_C
Cuts at: 2720 2888 3001 2720
Size: 168 113 6020
Fragments arranged by size:
6020 168 113
NheI G'CTAG_C
Cuts at: 1052 2746 1052
Size: 1694 4607
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2149 2197 3659 5204 1132
Size: 97 920 48 1462 1545 2229
Fragments arranged by size:
2229 1545 1462 920 97 48
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PmlI CAC'GTG
Cuts at: 2946 2946
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 2212 2432 3308 2212
Size: 220 876 5205
Fragments arranged by size:
5205 876 220
PstI C_TGCA'G
Cuts at: 830 2082 2409 2559 3072 3653 830
Size: 1252 327 150 513 581 3478
Fragments arranged by size:
3478 1252 581 513 327 150
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2312 2410 2312
Size: 98 6203
SacII CC_GC'GG
Cuts at: 2016 2016
Size: 6301
SalI G'TCGA_C
Cuts at: 1936 3194 3643 1936
Size: 1258 449 4594
Fragments arranged by size:
4594 1258 449
SbfI CC_TGCA'GG
Cuts at: 2559 3653 2559
Size: 1094 5207
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 2078 2405 2499 2555 3068 3649 4784
Size: 219 1033 327 94 56 513 581 1135
Cuts at: 4784 5465 5656 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1033 681 581 513 327 219
191 94 56
SfoI GGC'GCC
Cuts at: 2102 3109 2102
Size: 1007 5294
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 2036 3628 1872
Size: 164 1592 4545
Fragments arranged by size:
4545 1592 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2425 4405 5306 5568 820
Size: 197 926 482 1980 901 262 1553
Fragments arranged by size:
1980 1553 926 901 482 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SrfI GCCC'GGGC
Cuts at: 2036 2036
Size: 6301
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 3361 3740 513
Size: 552 939 390 967 379 3074
Fragments arranged by size:
3074 967 939 552 390 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4328 4345 4498 4683 5128 325
Size: 1255 2748 17 153 185 445 1498
Fragments arranged by size:
2748 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4547 6259
Size: 274 80 33 51 1443 174 2396 1712
Cuts at: 6259 96
Size: 138
Fragments arranged by size:
2396 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2209 2241 2667 2699 6261 6293 2209
Size: 32 426 32 3562 32 2217
Fragments arranged by size:
3562 2217 426 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2833 3069 2833
Size: 236 6065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 2034 3626 1870
Size: 164 1592 4545
Fragments arranged by size:
4545 1592 164
XmnI GAAnn'nnTTC
Cuts at: 4430 4430
Size: 6301
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4109 276
Size: 53 83 186 936 2575 2468
Fragments arranged by size:
2575 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SwaI