Restriction map for
pFN21AE6494
(Circular) (Six-base) MAPSORT of: pFN21AE6494.seq Check: 4279 from: 1 to: 6556
pFN21AE6494 6556 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3372 4366 278
Size: 53 83 186 936 1836 994 2468
Fragments arranged by size:
2468 1836 994 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3018 3899 1937
Size: 1081 881 4594
Fragments arranged by size:
4594 1081 881
Acc65I G'GTAC_C
Cuts at: 2008 3877 2008
Size: 1869 4687
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 4619 6003 6271 1632
Size: 554 2433 1384 268 1917
Fragments arranged by size:
2433 1917 1384 554 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2598 5455 6432 1203
Size: 1395 2857 977 1327
Fragments arranged by size:
2857 1395 1327 977
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2655 1184
Size: 1471 5085
AlwNI CAG_nnn'CTG
Cuts at: 3362 4299 5871 3362
Size: 937 1572 4047
Fragments arranged by size:
4047 1572 937
ApaI G_GGCC'C
Cuts at: 2033 2033
Size: 6556
ApaLI G'TGCA_C
Cuts at: 4613 5769 4613
Size: 1156 5400
ApoI r'AATT_y
Cuts at: 1493 3865 4091 4243 6352 1493
Size: 2372 226 152 2109 1697
Fragments arranged by size:
2372 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 3877 5332 618
Size: 325 217 627 221 1869 1455 1842
Fragments arranged by size:
1869 1842 1455 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2033 2197 2258 2275 3875 1391
Size: 642 164 61 17 1600 4072
Fragments arranged by size:
4072 1600 642 164 61 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5585
BbvCI CC'TCA_GC
Cuts at: 3304 3304
Size: 6556
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4747 4781 1903
Size: 34 2810 34 3678
Fragments arranged by size:
3678 2810 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 2760 1548
Size: 1212 5344
BfrBI ATG'CAT
Cuts at: 2906 2906
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5167 136
Size: 107 122 71 631 605 3495 1525
Fragments arranged by size:
3495 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3471 6551 3471
Size: 3080 3476
BlpI GC'TnA_GC
Cuts at: 3464 3973 3464
Size: 509 6047
Bme1580I G_kGCm'C
Cuts at: 1790 2033 4617 5773 1790
Size: 243 2584 1156 2573
Fragments arranged by size:
2584 2573 1156 243
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2028 2976 3535
Size: 416 53 83 399 24 611 948 559
Cuts at: 3535 4287 5245 442
Size: 752 958 1753
Fragments arranged by size:
1753 958 948 752 611 559 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2706 2955 3698 1056
Size: 1650 249 743 3914
Fragments arranged by size:
3914 1650 743 249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3831 5216 1239
Size: 2592 1385 2579
Fragments arranged by size:
2592 2579 1385
Bpu10I CC'TnA_GC
Cuts at: 2225 3304 3397 2225
Size: 1079 93 5384
Fragments arranged by size:
5384 1079 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4681 5546 5844 4681
Size: 865 298 5393
Fragments arranged by size:
5393 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2434 3213 3845 5219 882
Size: 617 935 779 632 1374 2219
Fragments arranged by size:
2219 1374 935 779 632 617
BsaAI yAC'GTr
Cuts at: 493 1204 4347 493
Size: 711 3143 2702
Fragments arranged by size:
3143 2702 711
BsaBI GATnn'nnATC
Cuts at: 2531 2784 6550 2531
Size: 253 3766 2537
Fragments arranged by size:
3766 2537 253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3369 4363 4745 5380
Size: 53 83 186 936 1836 994 382 635
Cuts at: 5380 275
Size: 1451
Fragments arranged by size:
1836 1451 994 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2291 4989 5387 5661 5808 6219 2291
Size: 2698 398 274 147 411 2628
Fragments arranged by size:
2698 2628 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6331
Fragments arranged by size:
6331 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 3046 3530 1186
Size: 1286 574 484 4212
Fragments arranged by size:
4212 1286 574 484
BseYI C'CCAG_C
Cuts at: 3373 5759 6255 3373
Size: 2386 496 3674
Fragments arranged by size:
3674 2386 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5983
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2258 2731 3465 4617 4702 5773 2258
Size: 473 734 1152 85 1071 3041
Fragments arranged by size:
3041 1152 1071 734 473 85
BsmI GAATG_Cn'
Cuts at: 2538 4067 4160 2538
Size: 1529 93 4934
Fragments arranged by size:
4934 1529 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2060 3290 2060
Size: 1230 5326
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2197 2258 2275 2731 3465 3875
Size: 399 243 164 61 17 456 734 410
Cuts at: 3875 4617 4702 5773 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 734 456 410 399 243
164 85 61 17
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 3612 4445 6175 3612
Size: 833 1730 3993
Fragments arranged by size:
3993 1730 833
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3911 844
Size: 3067 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2415 2448 2649 2947 5051 5225 65
Size: 1183 1167 33 201 298 2104 174 1396
Fragments arranged by size:
2104 1396 1183 1167 298 201 174 33
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 5200 5387 1700
Size: 110 275 3115 187 2869
Fragments arranged by size:
3115 2869 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 2869 96
Size: 2773 3783
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 4616 5628 4616
Size: 1012 5544
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2477 794
Size: 1683 4873
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 3079 1184
Size: 1895 4661
BstXI CCAn_nnnn'nTGG
Cuts at: 3453 3453
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 2803 3471 3886 4253 4638 4655 6107 6193
Size: 831 668 415 367 385 17 1452 86
Cuts at: 6193 6205 6551 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 831 668 415 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2584 3084 3501 3587 513
Size: 552 939 57 523 500 417 86 3482
Fragments arranged by size:
3482 939 552 523 500 417 86 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2452 487
Size: 683 56 1226 4591
Fragments arranged by size:
4591 1226 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 4072 4866 4886 2139
Size: 1933 794 20 3809
Fragments arranged by size:
3809 1933 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2953 2988 2995 3030 3799 3834 529
Size: 35 2389 35 7 35 769 35 3251
Fragments arranged by size:
3251 2389 769 35 35 35 35 7
DraI TTT'AAA
Cuts at: 3860 3860
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2881 4003 4892
Size: 54 1425 50 275 18 1051 1122 889
Cuts at: 4892 6521 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1122 1051 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2202 2449 2488 3015 4486 1353
Size: 849 247 39 527 1471 3423
Fragments arranged by size:
3423 1471 849 527 247 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5143 5517 5663 387
Size: 1064 138 3554 374 146 1280
Fragments arranged by size:
3554 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2256 2256
Size: 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 3831 4619 5216 6003 6271 1239
Size: 393 554 1645 788 597 787 268 1524
Fragments arranged by size:
1645 1524 788 787 597 554 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2029 2030 2299 2029
Size: 1 269 6286
Fragments arranged by size:
6286 269 1
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2467 2499 5197 5229 5271
Size: 32 135 32 895 32 2698 32 42
Cuts at: 5271 5303 1373
Size: 32 2626
Fragments arranged by size:
2698 2626 895 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3198 3900 4146 669
Size: 988 281 1260 702 246 3079
Fragments arranged by size:
3079 1260 988 702 281 246
HindIII A'AGCT_T
Cuts at: 748 2365 748
Size: 1617 4939
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 2012 3881 2012
Size: 1869 4687
MfeI C'AATT_G
Cuts at: 2637 3432 4155 2637
Size: 795 723 5038
Fragments arranged by size:
5038 795 723
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2908 2953 5670 5854 1305
Size: 1603 45 2717 184 2007
Fragments arranged by size:
2717 2007 1603 184 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2883 10
Size: 54 1425 343 1051 3683
Fragments arranged by size:
3683 1425 1051 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3110 3592 4514 4873 5032 518
Size: 648 1944 482 922 359 159 2042
Fragments arranged by size:
2042 1944 922 648 482 359 159
MspA1I CmG'CkG
Cuts at: 1606 2079 3972 4649 5797 6042 1606
Size: 473 1893 677 1148 245 2120
Fragments arranged by size:
2120 1893 1148 677 473 245
NaeI GCC'GGC
Cuts at: 2087 2087
Size: 6556
NcoI C'CATG_G
Cuts at: 513 1065 2004 2584 3084 3501 3587 513
Size: 552 939 580 500 417 86 3482
Fragments arranged by size:
3482 939 580 552 500 417 86
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NgoMIV G'CCGG_C
Cuts at: 2085 2085
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 2702 2951 3694 1052
Size: 1650 249 743 3914
Fragments arranged by size:
3914 1650 743 249
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NsiI A_TGCA'T
Cuts at: 2908 2908
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2119 2602 2830 3914 5459 1132
Size: 97 890 483 228 1084 1545 2229
Fragments arranged by size:
2229 1545 1084 890 483 228 97
PasI CC'CwG_GG
Cuts at: 1348 2097 3362 1348
Size: 749 1265 4542
Fragments arranged by size:
4542 1265 749
PciI A'CATG_T
Cuts at: 2598 5455 2598
Size: 2857 3699
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3160 1108
Size: 2052 4504
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2299
Size: 6556
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PspOMI G'GGCC_C
Cuts at: 2029 2029
Size: 6556
PstI C_TGCA'G
Cuts at: 830 3134 3908 830
Size: 2304 774 3478
Fragments arranged by size:
3478 2304 774
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
SacI G_AGCT'C
Cuts at: 2258 2258
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SapI GCTCTTCn'nnn_
Cuts at: 2202 2202
Size: 6556
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 4804 6516 4804
Size: 1712 4844
SfcI C'TryA_G
Cuts at: 826 1045 3130 3904 5039 5720 5911 826
Size: 219 2085 774 1135 681 191 1471
Fragments arranged by size:
2085 1471 1135 774 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4660 5561 5823 820
Size: 197 926 2717 901 262 1553
Fragments arranged by size:
2717 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 2857 152
Size: 2705 3851
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 4480 5
Size: 47 4428 2081
Fragments arranged by size:
4428 2081 47
StuI AGG'CCT
Cuts at: 1517 2499 1517
Size: 982 5574
StyI C'CwwG_G
Cuts at: 513 1065 2004 2584 2619 3084 3159 3501 3587
Size: 552 939 580 35 465 75 342 86
Cuts at: 3587 3995 513
Size: 408 3074
Fragments arranged by size:
3074 939 580 552 465 408 342 86
75 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3137 3580 4583 4600 4753 4938 5383
Size: 1255 1557 443 1003 17 153 185 445
Cuts at: 5383 325
Size: 1498
Fragments arranged by size:
1557 1498 1255 1003 445 443 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2595 2790 2869
Size: 274 80 33 51 1443 618 195 79
Cuts at: 2869 4802 6514 96
Size: 1933 1712 138
Fragments arranged by size:
1933 1712 1443 618 274 195 138 80
79 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6516 6548 6516
Size: 32 6524
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6556
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 4685 4685
Size: 6556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3370 4364 276
Size: 53 83 186 936 1836 994 2468
Fragments arranged by size:
2468 1836 994 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI