Restriction map for
pFN21AE6491
(Circular) (Six-base) MAPSORT of: pFN21AE6491.seq Check: 2839 from: 1 to: 6505
pFN21AE6491 6505 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2777 2837 3151 2777
Size: 60 314 6131
Fragments arranged by size:
6131 314 60
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4315 278
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2679 3848 1937
Size: 742 1169 4594
Fragments arranged by size:
4594 1169 742
Acc65I G'GTAC_C
Cuts at: 3826 3826
Size: 6505
AclI AA'CG_TT
Cuts at: 1563 2349 4632 5005 1563
Size: 786 2283 373 3063
Fragments arranged by size:
3063 2283 786 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3782 4568 5952 6220 1632
Size: 2150 786 1384 268 1917
Fragments arranged by size:
2150 1917 1384 786 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6308
AflIII A'CryG_T
Cuts at: 1203 5404 6381 1203
Size: 4201 977 1327
Fragments arranged by size:
4201 1327 977
AgeI A'CCGG_T
Cuts at: 5336 5336
Size: 6505
AhdI GACnn_n'nnGTC
Cuts at: 5234 5234
Size: 6505
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6505
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6505
AlwNI CAG_nnn'CTG
Cuts at: 2829 2898 3066 3325 3645 4248 5820 2829
Size: 69 168 259 320 603 1572 3514
Fragments arranged by size:
3514 1572 603 320 259 168 69
ApaLI G'TGCA_C
Cuts at: 4562 5718 4562
Size: 1156 5349
ApoI r'AATT_y
Cuts at: 1493 3814 4040 4192 6301 1493
Size: 2321 226 152 2109 1697
Fragments arranged by size:
2321 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5059 160
Size: 872 4027 1606
Fragments arranged by size:
4027 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
AvaI C'yCGr_G
Cuts at: 1870 1943 2063 2181 3114 3830 1870
Size: 73 120 118 933 716 4545
Fragments arranged by size:
4545 933 716 120 118 73
BamHI G'GATC_C
Cuts at: 3835 4202 3835
Size: 367 6138
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2839 3109 3139 3694
Size: 325 217 627 221 831 270 30 555
Cuts at: 3694 3826 5281 618
Size: 132 1455 1842
Fragments arranged by size:
1842 1455 831 627 555 325 270 221
217 132 30
BanII G_rGCy'C
Cuts at: 1391 2611 2862 2870 2943 3087 3653 3824 1391
Size: 1220 251 8 73 144 566 171 4072
Fragments arranged by size:
4072 1220 566 251 171 144 73 8
BbeI G_GCGC'C
Cuts at: 2012 2843 2012
Size: 831 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2458 928
Size: 971 559 4975
Fragments arranged by size:
4975 971 559
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4696 4730 1903
Size: 34 2759 34 3678
Fragments arranged by size:
3678 2759 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4397 5607 4397
Size: 1210 5295
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6505
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3002 3137 5116
Size: 107 122 71 631 605 1330 135 1979
Cuts at: 5116 136
Size: 1525
Fragments arranged by size:
1979 1525 1330 631 605 135 122 107
71
BglII A'GATC_T
Cuts at: 2403 3240 6500 2403
Size: 837 3260 2408
Fragments arranged by size:
3260 2408 837
BlpI GC'TnA_GC
Cuts at: 3922 3922
Size: 6505
Bme1580I G_kGCm'C
Cuts at: 1790 3047 4566 5722 1790
Size: 1257 1519 1156 2573
Fragments arranged by size:
2573 1519 1257 1156
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 5713
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4236 5194 442
Size: 416 53 83 399 24 2819 958 1753
Fragments arranged by size:
2819 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 3212 3461 3667 5165 1239
Size: 905 1068 249 206 1498 2579
Fragments arranged by size:
2579 1498 1068 905 249 206
Bpu10I CC'TnA_GC
Cuts at: 2856 2937 2856
Size: 81 6424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2099 2183 2348 2699 3539 4630 5495 5793 2099
Size: 84 165 351 840 1091 865 298 2811
Fragments arranged by size:
2811 1091 865 840 351 298 165 84
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2613 2937 5168 882
Size: 617 1114 324 2231 2219
Fragments arranged by size:
2231 2219 1114 617 324
BsaAI yAC'GTr
Cuts at: 493 1204 3792 4296 493
Size: 711 2588 504 2702
Fragments arranged by size:
2702 2588 711 504
BsaBI GATnn'nnATC
Cuts at: 6499 6499
Size: 6505
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2448 2840 4312
Size: 53 83 186 936 476 439 392 1472
Cuts at: 4312 4694 5329 275
Size: 382 635 1451
Fragments arranged by size:
1472 1451 936 635 476 439 392 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2166 3197 4938 5336 5610 5757 6168 2166
Size: 1031 1741 398 274 147 411 2503
Fragments arranged by size:
2503 1741 1031 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2261 2291 2294 2324 3209
Size: 30 165 30 858 30 3 30 885
Cuts at: 3209 3227 3239 3257 1178
Size: 18 12 18 4426
Fragments arranged by size:
4426 885 858 165 30 30 30 30
18 18 12 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 3530 1186
Size: 1228 1116 4161
Fragments arranged by size:
4161 1228 1116
BseYI C'CCAG_C
Cuts at: 2012 2042 2441 2891 2914 5708 6204 2012
Size: 30 399 450 23 2794 496 2313
Fragments arranged by size:
2794 2313 496 450 399 30 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2642 3728 1163
Size: 573 906 1086 3940
Fragments arranged by size:
3940 1086 906 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2266 3712 3955 4716 5744 1540
Size: 275 186 265 1446 243 761 1028 2301
Fragments arranged by size:
2301 1446 1028 761 275 265 243 186
BsiHKAI G_wGCw'C
Cuts at: 2109 3653 3758 4566 4651 5722 2109
Size: 1544 105 808 85 1071 2892
Fragments arranged by size:
2892 1544 1071 808 105 85
BsmI GAATG_Cn'
Cuts at: 2632 4016 4109 2632
Size: 1384 93 5028
Fragments arranged by size:
5028 1384 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2776 3098 2776
Size: 322 6183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2109 2611 2862 2870 2943 3047 3087
Size: 399 319 502 251 8 73 104 40
Cuts at: 3087 3653 3758 3824 4566 4651 5722 1391
Size: 566 105 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 566 502 399 319 251
105 104 85 73 66 40 8
BspEI T'CCGG_A
Cuts at: 2166 3197 6168 2166
Size: 1031 2971 2503
Fragments arranged by size:
2971 2503 1031
BspHI T'CATG_A
Cuts at: 2502 3681 4394 6124 2502
Size: 1179 713 1730 2883
Fragments arranged by size:
2883 1730 1179 713
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2368 2777 2837 3151 3291 3860 844
Size: 1524 409 60 314 140 569 3489
Fragments arranged by size:
3489 1524 569 409 314 140 60
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 2049 2128 2484 3301 4392 1394
Size: 429 206 20 79 356 817 1091 3507
Fragments arranged by size:
3507 1091 817 429 356 206 79 20
BsrDI GCAATG_nn'
Cuts at: 65 1248 2952 5000 5174 65
Size: 1183 1704 2048 174 1396
Fragments arranged by size:
2048 1704 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5149 5336 1700
Size: 110 3339 187 2869
Fragments arranged by size:
3339 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6505
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6505
BssSI C'ACGA_G
Cuts at: 4565 5577 4565
Size: 1012 5493
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3066 3666 794
Size: 2272 600 3633
Fragments arranged by size:
3633 2272 600
BstBI TT'CG_AA
Cuts at: 3354 5309 3354
Size: 1955 4550
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6505
BstYI r'GATC_y
Cuts at: 1972 2403 3240 3582 3835 4202 4587 4604 6056
Size: 431 837 342 253 367 385 17 1452
Cuts at: 6056 6142 6154 6500 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 837 431 385 367 346 342
253 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2904 3021 2904
Size: 117 6388
BtgI C'CryG_G
Cuts at: 513 1065 2004 2556 513
Size: 552 939 552 4462
Fragments arranged by size:
4462 939 552 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5766
Fragments arranged by size:
5766 683 56
BtsI GCAGTG_nn'
Cuts at: 2299 3646 4021 4815 4835 2299
Size: 1347 375 794 20 3969
Fragments arranged by size:
3969 1347 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6505
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6470
DraI TTT'AAA
Cuts at: 3809 3809
Size: 6505
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2546 5512 809
Size: 507 1230 2966 1802
Fragments arranged by size:
2966 1802 1230 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2263 2487 2559
Size: 54 1425 50 275 18 433 224 72
Cuts at: 2559 3274 3709 3952 4841 6470 8
Size: 715 435 243 889 1629 43
Fragments arranged by size:
1629 1425 889 715 435 433 275 243
224 72 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2263 3709 3952 1537
Size: 275 451 1446 243 4090
Fragments arranged by size:
4090 1446 451 275 243
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 4435 1353
Size: 815 2267 3423
Fragments arranged by size:
3423 2267 815
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2978 3368 5092 5466 5612 387
Size: 1064 138 1389 390 1724 374 146 1280
Fragments arranged by size:
1724 1389 1280 1064 390 374 146 138
EcoICRI GAG'CTC
Cuts at: 3651 3651
Size: 6505
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 3212 3461 3667 3782 4568 5165
Size: 393 512 1068 249 206 115 786 597
Cuts at: 5165 5952 6220 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1068 787 786 597 512 393 268
249 206 115
EcoNI CCTnn'n_nnAGG
Cuts at: 3614 3614
Size: 6505
EcoO109I rG'GnC_Cy
Cuts at: 3016 3050 3466 3568 3016
Size: 34 416 102 5953
Fragments arranged by size:
5953 416 102 34
EcoRI G'AATT_C
Cuts at: 3814 4192 3814
Size: 378 6127
FspI TGC'GCA
Cuts at: 3075 5011 3075
Size: 1936 4569
FspAI rTGC'GCAy
Cuts at: 3075 3075
Size: 6505
HaeII r_GCGC'y
Cuts at: 1678 2012 2843 3743 5652 1678
Size: 334 831 900 1909 2531
Fragments arranged by size:
2531 1909 900 831 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2294 2326 3227 3259 5146
Size: 32 135 32 722 32 901 32 1887
Cuts at: 5146 5178 5220 5252 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1887 901 722 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3166 3721 3849 4095 669
Size: 988 281 1228 555 128 246 3079
Fragments arranged by size:
3079 1228 988 555 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6505
HpaI GTT'AAC
Cuts at: 4095 4095
Size: 6505
KasI G'GCGC_C
Cuts at: 2008 2839 2008
Size: 831 5674
KpnI G_GTAC'C
Cuts at: 3830 3830
Size: 6505
MfeI C'AATT_G
Cuts at: 4104 4104
Size: 6505
MluI A'CGCG_T
Cuts at: 6381 6381
Size: 6505
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3207 3553 5619 5803 1305
Size: 1902 346 2066 184 2007
Fragments arranged by size:
2066 2007 1902 346 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4683
Fragments arranged by size:
4683 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2507 2513 3680 4463 4822 4981 518
Size: 648 1341 6 1167 783 359 159 2042
Fragments arranged by size:
2042 1341 1167 783 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2037 2122 2142 2580 3066 3636 3708 3921
Size: 431 85 20 438 486 570 72 213
Cuts at: 3921 4598 5746 5991 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 570 486 438 431 245
213 85 72 20
NarI GG'CG_CC
Cuts at: 2009 2840 2009
Size: 831 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5014
Fragments arranged by size:
5014 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6505
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6505
NotI GC'GGCC_GC
Cuts at: 2263 3709 3952 2263
Size: 1446 243 4816
Fragments arranged by size:
4816 1446 243
NspI r_CATG'y
Cuts at: 1132 1229 3863 5408 1132
Size: 97 2634 1545 2229
Fragments arranged by size:
2634 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6505
PciI A'CATG_T
Cuts at: 5404 5404
Size: 6505
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6505
PfoI T'CCnGG_A
Cuts at: 2391 2391
Size: 6505
PmeI GTTT'AAAC
Cuts at: 3809 3809
Size: 6505
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4574 4606 6087 6119 4574
Size: 32 1481 32 4960
Fragments arranged by size:
4960 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3016 3050 3466 3568 3016
Size: 34 416 102 5953
Fragments arranged by size:
5953 416 102 34
PshAI GACnn'nnGTC
Cuts at: 1613 3308 3617 1613
Size: 1695 309 4501
Fragments arranged by size:
4501 1695 309
PsiI TTA'TAA
Cuts at: 4075 4075
Size: 6505
PstI C_TGCA'G
Cuts at: 830 3857 830
Size: 3027 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6505
PvuII CAG'CTG
Cuts at: 2122 2122
Size: 6505
SacI G_AGCT'C
Cuts at: 3653 3653
Size: 6505
SalI G'TCGA_C
Cuts at: 1936 3847 1936
Size: 1911 4594
SanDI GG'GwC_CC
Cuts at: 3016 3016
Size: 6505
SbfI CC_TGCA'GG
Cuts at: 3857 3857
Size: 6505
ScaI AGT'ACT
Cuts at: 4753 6465 4753
Size: 1712 4793
SexAI A'CCwGG_T
Cuts at: 2670 2724 3462 2670
Size: 54 738 5713
Fragments arranged by size:
5713 738 54
SfcI C'TryA_G
Cuts at: 826 1045 2407 3853 4988 5669 5860 826
Size: 219 1362 1446 1135 681 191 1471
Fragments arranged by size:
1471 1446 1362 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3002 3002
Size: 6505
SfoI GGC'GCC
Cuts at: 2010 2841 2010
Size: 831 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6505
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6505
SmaI CCC'GGG
Cuts at: 1872 2065 2183 3116 3832 1872
Size: 193 118 933 716 4545
Fragments arranged by size:
4545 933 716 193 118
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2114 2198 2363 2714 3554 4609
Size: 197 926 171 84 165 351 840 1055
Cuts at: 4609 5510 5772 820
Size: 901 262 1553
Fragments arranged by size:
1553 1055 926 901 840 351 262 197
171 165 84
SnaBI TAC'GTA
Cuts at: 493 4296 493
Size: 3803 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6505
SphI G_CATG'C
Cuts at: 1132 3863 1132
Size: 2731 3774
SspI AAT'ATT
Cuts at: 5 52 4429 5
Size: 47 4377 2081
Fragments arranged by size:
4377 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6505
StyI C'CwwG_G
Cuts at: 513 1065 2004 2454 2964 3006 3944 513
Size: 552 939 450 510 42 938 3074
Fragments arranged by size:
3074 939 938 552 510 450 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2628 4532 4549 4702 4887 5332 325
Size: 1255 1048 1904 17 153 185 445 1498
Fragments arranged by size:
1904 1498 1255 1048 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 3180 4751
Size: 274 80 33 51 1443 354 849 1571
Cuts at: 4751 6463 96
Size: 1712 138
Fragments arranged by size:
1712 1571 1443 849 354 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6465 6497 6465
Size: 32 6473
Tth111I GACn'n_nGTC
Cuts at: 1316 3431 3677 1316
Size: 2115 246 4144
Fragments arranged by size:
4144 2115 246
XbaI T'CTAG_A
Cuts at: 3841 3841
Size: 6505
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2671 2752 2971 3556 2671
Size: 81 219 585 5620
Fragments arranged by size:
5620 585 219 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6505
XmaI C'CCGG_G
Cuts at: 1870 2063 2181 3114 3830 1870
Size: 193 118 933 716 4545
Fragments arranged by size:
4545 933 716 193 118
XmnI GAAnn'nnTTC
Cuts at: 4634 4634
Size: 6505
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4313 276
Size: 53 83 186 936 2779 2468
Fragments arranged by size:
2779 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI
NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SapI SrfI SwaI