Restriction map for
pFN21AE6489
(Circular) (Six-base) MAPSORT of: pFN21AE6489.seq Check: 9589 from: 1 to: 6484
pFN21AE6489 6484 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3461 3461
Size: 6484
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4294 278
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3051 3827 1937
Size: 1114 776 4594
Fragments arranged by size:
4594 1114 776
Acc65I G'GTAC_C
Cuts at: 3805 3805
Size: 6484
AclI AA'CG_TT
Cuts at: 1563 4611 4984 1563
Size: 3048 373 3063
Fragments arranged by size:
3063 3048 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2631 4547 5931 6199 1632
Size: 999 1916 1384 268 1917
Fragments arranged by size:
1917 1916 1384 999 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6287
AflIII A'CryG_T
Cuts at: 1203 2734 3594 5383 6360 1203
Size: 1531 860 1789 977 1327
Fragments arranged by size:
1789 1531 1327 977 860
AgeI A'CCGG_T
Cuts at: 5315 5315
Size: 6484
AhdI GACnn_n'nnGTC
Cuts at: 5213 5213
Size: 6484
AleI CACnn'nnGTG
Cuts at: 1166 2183 3385 1166
Size: 1017 1202 4265
Fragments arranged by size:
4265 1202 1017
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2574 1184
Size: 1390 5094
AlwNI CAG_nnn'CTG
Cuts at: 2402 4227 5799 2402
Size: 1825 1572 3087
Fragments arranged by size:
3087 1825 1572
ApaLI G'TGCA_C
Cuts at: 4541 5697 4541
Size: 1156 5328
ApoI r'AATT_y
Cuts at: 1493 2161 2202 2358 2444 2582 3793 4019 4171
Size: 668 41 156 86 138 1211 226 152
Cuts at: 4171 6280 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1211 668 226 156 152 138
86 41
AseI AT'TA_AT
Cuts at: 160 1032 2876 5038 160
Size: 872 1844 2162 1606
Fragments arranged by size:
2162 1844 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
AvaI C'yCGr_G
Cuts at: 1870 1943 2757 3809 1870
Size: 73 814 1052 4545
Fragments arranged by size:
4545 1052 814 73
BamHI G'GATC_C
Cuts at: 3814 4181 3814
Size: 367 6117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3003 3805 5260 618
Size: 325 217 627 1216 802 1455 1842
Fragments arranged by size:
1842 1455 1216 802 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3803 1391
Size: 2412 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 2438 928
Size: 971 300 239 4974
Fragments arranged by size:
4974 971 300 239
BbvCI CC'TCA_GC
Cuts at: 2900 2900
Size: 6484
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4675 4709 1903
Size: 34 2738 34 3678
Fragments arranged by size:
3678 2738 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4376 5586 4376
Size: 1210 5274
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6484
BfrBI ATG'CAT
Cuts at: 2506 2510 2506
Size: 4 6480
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5095 136
Size: 107 122 71 631 605 3423 1525
Fragments arranged by size:
3423 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6479 6479
Size: 6484
BlpI GC'TnA_GC
Cuts at: 3901 3901
Size: 6484
Bme1580I G_kGCm'C
Cuts at: 1790 4545 5701 1790
Size: 2755 1156 2573
Fragments arranged by size:
2755 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3383 1318
Size: 2065 4419
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2090 4215 5173
Size: 416 53 83 399 24 673 2125 958
Cuts at: 5173 442
Size: 1753
Fragments arranged by size:
2125 1753 958 673 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6484
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3365
Size: 6484
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5144 1239
Size: 3905 2579
Bpu10I CC'TnA_GC
Cuts at: 2479 2900 2479
Size: 421 6063
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2608 4609 5474 5772 2608
Size: 2001 865 298 3320
Fragments arranged by size:
3320 2001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2411 3159 5147 882
Size: 617 912 748 1988 2219
Fragments arranged by size:
2219 1988 912 748 617
BsaAI yAC'GTr
Cuts at: 493 1204 4275 493
Size: 711 3071 2702
Fragments arranged by size:
3071 2702 711
BsaBI GATnn'nnATC
Cuts at: 6478 6478
Size: 6484
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 3677 4291 4673
Size: 53 83 186 936 479 1665 614 382
Cuts at: 4673 5308 275
Size: 635 1451
Fragments arranged by size:
1665 1451 936 635 614 479 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2889 2964 3168 4917 5315 5589 5736 6147 2889
Size: 75 204 1749 398 274 147 411 3226
Fragments arranged by size:
3226 1749 411 398 274 204 147 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3177 3207 3226 3256 3304
Size: 30 165 30 1774 30 19 30 48
Cuts at: 3304 3334 3459 3489 1178
Size: 30 125 30 4173
Fragments arranged by size:
4173 1774 165 125 48 30 30 30
30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3328 1186
Size: 2142 4342
BseYI C'CCAG_C
Cuts at: 2395 2457 5687 6183 2395
Size: 62 3230 496 2696
Fragments arranged by size:
3230 2696 496 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 3055 3619 3642 1163
Size: 573 896 423 564 23 4005
Fragments arranged by size:
4005 896 573 564 423 23
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3934 4695 5723 1540
Size: 275 186 1933 761 1028 2301
Fragments arranged by size:
2301 1933 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3455 3740 4545 4630 5701 3455
Size: 285 805 85 1071 4238
Fragments arranged by size:
4238 1071 805 285 85
BsmI GAATG_Cn'
Cuts at: 2981 3410 3995 4088 2981
Size: 429 585 93 5377
Fragments arranged by size:
5377 585 429 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2003 2003
Size: 6484
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3455 3740 3803 4545 4630 5701 1391
Size: 399 1665 285 63 742 85 1071 2174
Fragments arranged by size:
2174 1665 1071 742 399 285 85 63
BspEI T'CCGG_A
Cuts at: 2889 2964 6147 2889
Size: 75 3183 3226
Fragments arranged by size:
3226 3183 75
BspHI T'CATG_A
Cuts at: 2139 3514 4373 6103 2139
Size: 1375 859 1730 2520
Fragments arranged by size:
2520 1730 1375 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 3839 844
Size: 2617 378 3489
Fragments arranged by size:
3489 2617 378
BsrBI CCG'CTC
Cuts at: 1394 1823 4371 1394
Size: 429 2548 3507
Fragments arranged by size:
3507 2548 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2844 4979 5153 65
Size: 1183 1596 2135 174 1396
Fragments arranged by size:
2135 1596 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5128 5315 1700
Size: 110 3318 187 2869
Fragments arranged by size:
3318 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2380 96
Size: 2284 4200
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6484
BssSI C'ACGA_G
Cuts at: 4544 5556 4544
Size: 1012 5472
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2814 794
Size: 2020 4464
BstBI TT'CG_AA
Cuts at: 5288 5288
Size: 6484
BstEII G'GTnAC_C
Cuts at: 1184 2742 1184
Size: 1558 4926
BstXI CCAn_nnnn'nTGG
Cuts at: 2929 2929
Size: 6484
BstYI r'GATC_y
Cuts at: 1972 3814 4181 4566 4583 6035 6121 6133 6479
Size: 1842 367 385 17 1452 86 12 346
Cuts at: 6479 1972
Size: 1977
Fragments arranged by size:
1977 1842 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2324 2324
Size: 6484
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4993
Fragments arranged by size:
4993 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2187 487
Size: 683 56 961 4784
Fragments arranged by size:
4784 961 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2349 2815 3325 4000 4794 4814 2089
Size: 260 466 510 675 794 20 3759
Fragments arranged by size:
3759 794 675 510 466 260 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6484
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6449
DraI TTT'AAA
Cuts at: 2989 3788 2989
Size: 799 5685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3721 5491 809
Size: 507 2405 1770 1802
Fragments arranged by size:
2405 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 3931 4820
Size: 54 1425 50 275 18 396 1705 889
Cuts at: 4820 6449 8
Size: 1629 43
Fragments arranged by size:
1705 1629 1425 889 396 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3931 1537
Size: 275 2119 4090
Fragments arranged by size:
4090 2119 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2646 2778 2876 4414 1353
Size: 1293 132 98 1538 3423
Fragments arranged by size:
3423 1538 1293 132 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3475 5071 5445 5591 387
Size: 1064 138 1886 1596 374 146 1280
Fragments arranged by size:
1886 1596 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2631 4547 5144 5931 6199 1239
Size: 393 999 1916 597 787 268 1524
Fragments arranged by size:
1916 1524 999 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3793 4171 3793
Size: 378 6106
FspI TGC'GCA
Cuts at: 4990 4990
Size: 6484
HaeII r_GCGC'y
Cuts at: 1678 5631 1678
Size: 3953 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2092 2124 3362 3394 5125
Size: 32 135 32 520 32 1238 32 1731
Cuts at: 5125 5157 5199 5231 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1731 1238 520 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 3828 4074 669
Size: 988 281 535 1355 246 3079
Fragments arranged by size:
3079 1355 988 535 281 246
HindIII A'AGCT_T
Cuts at: 748 2127 748
Size: 1379 5105
HpaI GTT'AAC
Cuts at: 4074 4074
Size: 6484
KpnI G_GTAC'C
Cuts at: 3809 3809
Size: 6484
MfeI C'AATT_G
Cuts at: 2181 4083 2181
Size: 1902 4582
MluI A'CGCG_T
Cuts at: 6360 6360
Size: 6484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3319 3628 5598 5782 1305
Size: 2014 309 1970 184 2007
Fragments arranged by size:
2014 2007 1970 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4662
Fragments arranged by size:
4662 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3385 4442 4801 4960 518
Size: 648 1017 1202 1057 359 159 2042
Fragments arranged by size:
2042 1202 1057 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2402 3900 4577 5725 5970 1606
Size: 414 382 1498 677 1148 245 2120
Fragments arranged by size:
2120 1498 1148 677 414 382 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4993
Fragments arranged by size:
4993 939 552
NdeI CA'TA_TG
Cuts at: 387 2118 387
Size: 1731 4753
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6484
NotI GC'GGCC_GC
Cuts at: 3931 3931
Size: 6484
NsiI A_TGCA'T
Cuts at: 2508 2512 2508
Size: 4 6480
NspI r_CATG'y
Cuts at: 1132 1229 2034 2510 2514 2738 3598 3842 5387
Size: 97 805 476 4 224 860 244 1545
Cuts at: 5387 1132
Size: 2229
Fragments arranged by size:
2229 1545 860 805 476 244 224 97
4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6484
PciI A'CATG_T
Cuts at: 2734 3594 5383 2734
Size: 860 1789 3835
Fragments arranged by size:
3835 1789 860
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2402 1108
Size: 1294 5190
PfoI T'CCnGG_A
Cuts at: 3026 3026
Size: 6484
PmeI GTTT'AAAC
Cuts at: 3788 3788
Size: 6484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4553 4585 6066 6098 4553
Size: 32 1481 32 4939
Fragments arranged by size:
4939 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6484
PsiI TTA'TAA
Cuts at: 3693 4054 3693
Size: 361 6123
PstI C_TGCA'G
Cuts at: 830 2356 3836 830
Size: 1526 1480 3478
Fragments arranged by size:
3478 1526 1480
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6484
PvuII CAG'CTG
Cuts at: 2402 2402
Size: 6484
SalI G'TCGA_C
Cuts at: 1936 3826 1936
Size: 1890 4594
SbfI CC_TGCA'GG
Cuts at: 3836 3836
Size: 6484
ScaI AGT'ACT
Cuts at: 4732 6444 4732
Size: 1712 4772
SfcI C'TryA_G
Cuts at: 826 1045 2233 2352 3111 3357 3504 3832 4967
Size: 219 1188 119 759 246 147 328 1135
Cuts at: 4967 5648 5839 826
Size: 681 191 1471
Fragments arranged by size:
1471 1188 1135 759 681 328 246 219
191 147 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6484
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6484
SmaI CCC'GGG
Cuts at: 1872 3811 1872
Size: 1939 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2623 4588 5489 5751 820
Size: 197 926 680 1965 901 262 1553
Fragments arranged by size:
1965 1553 926 901 680 262 197
SnaBI TAC'GTA
Cuts at: 493 4275 493
Size: 3782 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6484
SphI G_CATG'C
Cuts at: 1132 2034 2510 3842 1132
Size: 902 476 1332 3774
Fragments arranged by size:
3774 1332 902 476
SspI AAT'ATT
Cuts at: 5 52 2765 3689 4408 5
Size: 47 2713 924 719 2081
Fragments arranged by size:
2713 2081 924 719 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6484
StyI C'CwwG_G
Cuts at: 513 1065 2004 2111 3923 513
Size: 552 939 107 1812 3074
Fragments arranged by size:
3074 1812 939 552 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2671 2923 3320 4511 4528 4681 4866
Size: 1255 1091 252 397 1191 17 153 185
Cuts at: 4866 5311 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1191 1091 445 397 252 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2380 2569 3236
Size: 274 80 33 51 1443 403 189 667
Cuts at: 3236 4730 6442 96
Size: 1494 1712 138
Fragments arranged by size:
1712 1494 1443 667 403 274 189 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3177 3209 3304 3336 6444 6476 3177
Size: 32 95 32 3108 32 3185
Fragments arranged by size:
3185 3108 95 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6484
XbaI T'CTAG_A
Cuts at: 3820 3820
Size: 6484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2929 2929
Size: 6484
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6484
XmaI C'CCGG_G
Cuts at: 1870 3809 1870
Size: 1939 4545
XmnI GAAnn'nnTTC
Cuts at: 2129 2583 4613 2129
Size: 454 2030 4000
Fragments arranged by size:
4000 2030 454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4292 276
Size: 53 83 186 936 2758 2468
Fragments arranged by size:
2758 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI