Restriction map for
pFN21AE6487
(Circular) (Six-base) MAPSORT of: pFN21AE6487.seq Check: 9948 from: 1 to: 6226
pFN21AE6487 6226 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4036 278
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3569 1937
Size: 1632 4594
Acc65I G'GTAC_C
Cuts at: 3547 3547
Size: 6226
AclI AA'CG_TT
Cuts at: 1563 4353 4726 1563
Size: 2790 373 3063
Fragments arranged by size:
3063 2790 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 2360 2870 4289 5673 5941 1632
Size: 557 171 510 1419 1384 268 1917
Fragments arranged by size:
1917 1419 1384 557 510 268 171
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6029
AflIII A'CryG_T
Cuts at: 1203 3030 5125 6102 1203
Size: 1827 2095 977 1327
Fragments arranged by size:
2095 1827 1327 977
AgeI A'CCGG_T
Cuts at: 5057 5057
Size: 6226
AhdI GACnn_n'nnGTC
Cuts at: 4955 4955
Size: 6226
AleI CACnn'nnGTG
Cuts at: 1166 2471 1166
Size: 1305 4921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6226
AlwNI CAG_nnn'CTG
Cuts at: 3316 3969 5541 3316
Size: 653 1572 4001
Fragments arranged by size:
4001 1572 653
ApaI G_GGCC'C
Cuts at: 2131 2131
Size: 6226
ApaLI G'TGCA_C
Cuts at: 3039 4283 5439 3039
Size: 1244 1156 3826
Fragments arranged by size:
3826 1244 1156
ApoI r'AATT_y
Cuts at: 1493 3071 3535 3761 3913 6022 1493
Size: 1578 464 226 152 2109 1697
Fragments arranged by size:
2109 1697 1578 464 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4780 160
Size: 872 3748 1606
Fragments arranged by size:
3748 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
AvaI C'yCGr_G
Cuts at: 1870 1943 2520 2642 3129 3551 1870
Size: 73 577 122 487 422 4545
Fragments arranged by size:
4545 577 487 422 122 73
BamHI G'GATC_C
Cuts at: 3319 3556 3923 3319
Size: 237 367 5622
Fragments arranged by size:
5622 367 237
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2320 3306 3547 5002 618
Size: 325 217 627 533 986 241 1455 1842
Fragments arranged by size:
1842 1455 986 627 533 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2122 2131 2481 2831 3545 1391
Size: 731 9 350 350 714 4072
Fragments arranged by size:
4072 731 714 350 350 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5255
BbvCI CC'TCA_GC
Cuts at: 2081 2780 2081
Size: 699 5527
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4417 4451 1903
Size: 34 2480 34 3678
Fragments arranged by size:
3678 2480 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4118 5328 4118
Size: 1210 5016
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6226
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4837 136
Size: 107 122 71 631 605 3165 1525
Fragments arranged by size:
3165 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6221 6221
Size: 6226
BlpI GC'TnA_GC
Cuts at: 3643 3643
Size: 6226
Bme1580I G_kGCm'C
Cuts at: 1790 2131 2220 3043 4287 5443 1790
Size: 341 89 823 1244 1156 2573
Fragments arranged by size:
2573 1244 1156 823 341 89
BmgBI CAC'GTC
Cuts at: 1318 3105 1318
Size: 1787 4439
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2795 3957 4915
Size: 416 53 83 399 24 1378 1162 958
Cuts at: 4915 442
Size: 1753
Fragments arranged by size:
1753 1378 1162 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3155
Size: 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 2372 3044 4886 1239
Size: 944 189 672 1842 2579
Fragments arranged by size:
2579 1842 944 672 189
Bpu10I CC'TnA_GC
Cuts at: 2081 2780 2081
Size: 699 5527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2932 4351 5216 5514 2932
Size: 1419 865 298 3644
Fragments arranged by size:
3644 1419 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 2177 2685 4889 882
Size: 617 513 165 508 2204 2219
Fragments arranged by size:
2219 2204 617 513 508 165
BsaAI yAC'GTr
Cuts at: 493 1204 4017 493
Size: 711 2813 2702
Fragments arranged by size:
2813 2702 711
BsaBI GATnn'nnATC
Cuts at: 6220 6220
Size: 6226
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2765 3048 4033 4415
Size: 53 83 186 936 1232 283 985 382
Cuts at: 4415 5050 275
Size: 635 1451
Fragments arranged by size:
1451 1232 985 936 635 382 283 186
83 53
BsaWI w'CCGG_w
Cuts at: 4659 5057 5331 5478 5889 4659
Size: 398 274 147 411 4996
Fragments arranged by size:
4996 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2540 2570 3185 3215 1178
Size: 30 165 30 1137 30 615 30 4189
Fragments arranged by size:
4189 1137 615 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6226
BseYI C'CCAG_C
Cuts at: 2062 3355 5429 5925 2062
Size: 1293 2074 496 2363
Fragments arranged by size:
2363 2074 1293 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 3011 3085 3439 1163
Size: 573 744 531 74 354 3950
Fragments arranged by size:
3950 744 573 531 354 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3676 4437 5465 1540
Size: 275 186 1675 761 1028 2301
Fragments arranged by size:
2301 1675 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2122 2635 3043 3234 4287 4372 5443 2122
Size: 513 408 191 1053 85 1071 2905
Fragments arranged by size:
2905 1071 1053 513 408 191 85
BsmI GAATG_Cn'
Cuts at: 3737 3830 3737
Size: 93 6133
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2122 2131 2220 2481 2635 2831 3043
Size: 399 332 9 89 261 154 196 212
Cuts at: 3043 3234 3545 4287 4372 5443 1391
Size: 191 311 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 332 311 261 212
196 191 154 89 85 9
BspEI T'CCGG_A
Cuts at: 5889 5889
Size: 6226
BspHI T'CATG_A
Cuts at: 4115 5845 4115
Size: 1730 4496
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3033 3246 3372 3581 844
Size: 2189 213 126 209 3489
Fragments arranged by size:
3489 2189 213 209 126
BsrBI CCG'CTC
Cuts at: 1394 1823 4113 1394
Size: 429 2290 3507
Fragments arranged by size:
3507 2290 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2661 2673 2814 4721 4895 65
Size: 1183 1413 12 141 1907 174 1396
Fragments arranged by size:
1907 1413 1396 1183 174 141 12
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 4870 5057 1700
Size: 110 442 2618 187 2869
Fragments arranged by size:
2869 2618 442 187 110
BsrGI T'GTAC_A
Cuts at: 96 3413 96
Size: 3317 2909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6226
BssSI C'ACGA_G
Cuts at: 4286 5298 4286
Size: 1012 5214
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2594 794
Size: 1800 4426
BstBI TT'CG_AA
Cuts at: 5030 5030
Size: 6226
BstEII G'GTnAC_C
Cuts at: 1184 2209 1184
Size: 1025 5201
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2473 2161
Size: 312 5914
BstYI r'GATC_y
Cuts at: 1972 2844 3319 3556 3923 4308 4325 5777 5863
Size: 872 475 237 367 385 17 1452 86
Cuts at: 5863 5875 6221 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 872 475 385 367 346 237
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2880 3381 513
Size: 552 939 876 501 3358
Fragments arranged by size:
3358 939 876 552 501
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5487
Fragments arranged by size:
5487 683 56
BtsI GCAGTG_nn'
Cuts at: 2572 2574 3331 3390 3742 4536 4556 2572
Size: 2 757 59 352 794 20 4242
Fragments arranged by size:
4242 794 757 352 59 20 2
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6226
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2656 2691 3363 3398 529
Size: 35 2092 35 672 35 3357
Fragments arranged by size:
3357 2092 672 35 35 35
DraI TTT'AAA
Cuts at: 3530 3530
Size: 6226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2191 5233 809
Size: 507 875 3042 1802
Fragments arranged by size:
3042 1802 875 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2877 2994 3673
Size: 54 1425 50 275 18 1047 117 679
Cuts at: 3673 4562 6191 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1047 889 679 275 117 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3673 1537
Size: 275 1861 4090
Fragments arranged by size:
4090 1861 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4156 1353
Size: 2803 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4813 5187 5333 387
Size: 1064 138 3224 374 146 1280
Fragments arranged by size:
3224 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2120 2120
Size: 6226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2189 2360 2372 2870 3044 4289
Size: 393 551 6 171 12 498 174 1245
Cuts at: 4289 4886 5673 5941 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1245 787 597 551 498 393 268
174 171 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2453 3191 3254 2453
Size: 738 63 5425
Fragments arranged by size:
5425 738 63
EcoO109I rG'GnC_Cy
Cuts at: 2127 2128 2809 3313 3361 3433 2127
Size: 1 681 504 48 72 4920
Fragments arranged by size:
4920 681 504 72 48 1
EcoRI G'AATT_C
Cuts at: 3535 3913 3535
Size: 378 5848
FspI TGC'GCA
Cuts at: 4732 4732
Size: 6226
HaeII r_GCGC'y
Cuts at: 1678 5373 1678
Size: 3695 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2617 2649 2682 2714 2892
Size: 32 135 32 1045 32 33 32 178
Cuts at: 2892 2924 3185 3217 3420 3452 4867 4899 4941
Size: 32 261 32 203 32 1415 32 42
Cuts at: 4941 4973 1373
Size: 32 2626
Fragments arranged by size:
2626 1415 1045 261 203 178 135 42
33 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2650 3570 3816 669
Size: 988 281 712 920 246 3079
Fragments arranged by size:
3079 988 920 712 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6226
HpaI GTT'AAC
Cuts at: 3816 3816
Size: 6226
KpnI G_GTAC'C
Cuts at: 3551 3551
Size: 6226
MfeI C'AATT_G
Cuts at: 3825 3825
Size: 6226
MluI A'CGCG_T
Cuts at: 6102 6102
Size: 6226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5340 5524 1305
Size: 4035 184 2007
Fragments arranged by size:
4035 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2879 2996 10
Size: 54 1425 343 1047 117 3240
Fragments arranged by size:
3240 1425 1047 343 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2456 2471 4184 4543 4702 518
Size: 648 1290 15 1713 359 159 2042
Fragments arranged by size:
2042 1713 1290 648 359 159 15
MspA1I CmG'CkG
Cuts at: 1606 2029 2043 2384 2770 3642 4319 5467 5712
Size: 423 14 341 386 872 677 1148 245
Cuts at: 5712 1606
Size: 2120
Fragments arranged by size:
2120 1148 872 677 423 386 341 245
14
NaeI GCC'GGC
Cuts at: 2254 2254
Size: 6226
NcoI C'CATG_G
Cuts at: 513 1065 2004 2880 513
Size: 552 939 876 3859
Fragments arranged by size:
3859 939 876 552
NdeI CA'TA_TG
Cuts at: 387 2969 387
Size: 2582 3644
NgoMIV G'CCGG_C
Cuts at: 2252 2252
Size: 6226
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6226
NotI GC'GGCC_GC
Cuts at: 3673 3673
Size: 6226
NspI r_CATG'y
Cuts at: 1132 1229 2260 3034 3584 5129 1132
Size: 97 1031 774 550 1545 2229
Fragments arranged by size:
2229 1545 1031 774 550 97
PasI CC'CwG_GG
Cuts at: 1348 2599 1348
Size: 1251 4975
PciI A'CATG_T
Cuts at: 3030 5125 3030
Size: 2095 4131
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3316 1108
Size: 2208 4018
PmeI GTTT'AAAC
Cuts at: 3530 3530
Size: 6226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4295 4327 5808 5840 4295
Size: 32 1481 32 4681
Fragments arranged by size:
4681 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 3361 2809
Size: 552 5674
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6226
PsiI TTA'TAA
Cuts at: 3796 3796
Size: 6226
PspOMI G'GGCC_C
Cuts at: 2127 2127
Size: 6226
PspXI vC'TCGA_Gb
Cuts at: 3129 3129
Size: 6226
PstI C_TGCA'G
Cuts at: 830 3578 830
Size: 2748 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6226
PvuII CAG'CTG
Cuts at: 2043 2384 2043
Size: 341 5885
SacI G_AGCT'C
Cuts at: 2122 2122
Size: 6226
SalI G'TCGA_C
Cuts at: 1936 3568 1936
Size: 1632 4594
SbfI CC_TGCA'GG
Cuts at: 3578 3578
Size: 6226
ScaI AGT'ACT
Cuts at: 4474 6186 4474
Size: 1712 4514
SexAI A'CCwGG_T
Cuts at: 3186 3186
Size: 6226
SfcI C'TryA_G
Cuts at: 826 1045 2100 2737 3574 4709 5390 5581 826
Size: 219 1055 637 837 1135 681 191 1471
Fragments arranged by size:
1471 1135 1055 837 681 637 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6226
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6226
SmaI CCC'GGG
Cuts at: 1872 3553 1872
Size: 1681 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2520 2911 3129 4330 5231 5493
Size: 197 926 577 391 218 1201 901 262
Cuts at: 5493 820
Size: 1553
Fragments arranged by size:
1553 1201 926 901 577 391 262 218
197
SnaBI TAC'GTA
Cuts at: 493 4017 493
Size: 3524 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6226
SphI G_CATG'C
Cuts at: 1132 2260 3584 1132
Size: 1128 1324 3774
Fragments arranged by size:
3774 1324 1128
SspI AAT'ATT
Cuts at: 5 52 4150 5
Size: 47 4098 2081
Fragments arranged by size:
4098 2081 47
StuI AGG'CCT
Cuts at: 1517 2202 2235 3249 1517
Size: 685 33 1014 4494
Fragments arranged by size:
4494 1014 685 33
StyI C'CwwG_G
Cuts at: 513 1065 2004 2541 2880 3665 513
Size: 552 939 537 339 785 3074
Fragments arranged by size:
3074 939 785 552 537 339
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2848 4253 4270 4423 4608 5053 325
Size: 1255 1268 1405 17 153 185 445 1498
Fragments arranged by size:
1498 1405 1268 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 3413 4472
Size: 274 80 33 51 1443 960 476 1059
Cuts at: 4472 6184 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1059 960 476 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 3033 3065 6186 6218 2540
Size: 32 461 32 3121 32 2548
Fragments arranged by size:
3121 2548 461 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6226
XbaI T'CTAG_A
Cuts at: 3562 3562
Size: 6226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2716 2716
Size: 6226
XhoI C'TCGA_G
Cuts at: 1943 2520 3129 1943
Size: 577 609 5040
Fragments arranged by size:
5040 609 577
XmaI C'CCGG_G
Cuts at: 1870 3551 1870
Size: 1681 4545
XmnI GAAnn'nnTTC
Cuts at: 3071 4355 3071
Size: 1284 4942
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4034 276
Size: 53 83 186 936 2500 2468
Fragments arranged by size:
2500 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI