Restriction map for
pFN21AE6483
(Circular) (Six-base) MAPSORT of: pFN21AE6483.seq Check: 394 from: 1 to: 6364
pFN21AE6483 6364 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3362 3362
Size: 6364
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3707 1937
Size: 1770 4594
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2456 2531 2710 4427 5811 6079 1632
Size: 824 75 179 1717 1384 268 1917
Fragments arranged by size:
1917 1717 1384 824 268 179 75
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 5263 6240 1203
Size: 4060 977 1327
Fragments arranged by size:
4060 1327 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 5093 5093
Size: 6364
AleI CACnn'nnGTG
Cuts at: 1166 2396 3278 1166
Size: 1230 882 4252
Fragments arranged by size:
4252 1230 882
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2543 1184
Size: 1359 5005
AlwNI CAG_nnn'CTG
Cuts at: 2899 3124 4107 5679 2899
Size: 225 983 1572 3584
Fragments arranged by size:
3584 1572 983 225
ApaI G_GGCC'C
Cuts at: 2246 2692 2246
Size: 446 5918
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 3673 3899 4051 6160 1493
Size: 2180 226 152 2109 1697
Fragments arranged by size:
2180 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 3551 3689 1870
Size: 73 1608 138 4545
Fragments arranged by size:
4545 1608 138 73
AvrII C'CTAG_G
Cuts at: 2194 2194
Size: 6364
BamHI G'GATC_C
Cuts at: 3694 4061 3694
Size: 367 5997
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2155 3017 3188 3280 3514
Size: 325 217 627 368 862 171 92 234
Cuts at: 3514 3685 5140 618
Size: 171 1455 1842
Fragments arranged by size:
1842 1455 862 627 368 325 234 217
171 171 92
BanII G_rGCy'C
Cuts at: 1391 2246 2692 3258 3301 3381 3683 1391
Size: 855 446 566 43 80 302 4072
Fragments arranged by size:
4072 855 566 446 302 80 43
BbeI G_GCGC'C
Cuts at: 3192 3192
Size: 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4555 4589 1903
Size: 34 2618 34 3678
Fragments arranged by size:
3678 2618 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 2290 3213
Size: 107 122 71 631 605 550 68 923
Cuts at: 3213 4975 136
Size: 1762 1525
Fragments arranged by size:
1762 1525 923 631 605 550 122 107
71 68
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 2987 3203 3307 3781 2987
Size: 216 104 474 5570
Fragments arranged by size:
5570 474 216 104
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2158 2246 2660 2692 3020 3285 4425
Size: 349 19 88 414 32 328 265 1140
Cuts at: 4425 5581 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1140 414 349 328 265 88
32 19
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2443 4095 5053
Size: 416 53 83 399 24 1026 1652 958
Cuts at: 5053 442
Size: 1753
Fragments arranged by size:
1753 1652 1026 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2135 2347 2557 5024 1239
Size: 806 90 212 210 2467 2579
Fragments arranged by size:
2579 2467 806 212 210 90
Bpu10I CC'TnA_GC
Cuts at: 2181 3395 2181
Size: 1214 5150
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2224 4489 5354 5652 2224
Size: 2265 865 298 2936
Fragments arranged by size:
2936 2265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2086 2803 3548 5027 882
Size: 617 587 717 745 1479 2219
Fragments arranged by size:
2219 1479 745 717 617 587
BsaAI yAC'GTr
Cuts at: 493 1204 4155 493
Size: 711 2951 2702
Fragments arranged by size:
2951 2702 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2640 3189 4171 4553
Size: 53 83 186 936 1107 549 982 382
Cuts at: 4553 5188 275
Size: 635 1451
Fragments arranged by size:
1451 1107 982 936 635 549 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3048 4797 5195 5469 5616 6027 3048
Size: 1749 398 274 147 411 3385
Fragments arranged by size:
3385 1749 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2191 2221 2844 2874 1178
Size: 30 165 30 788 30 623 30 4668
Fragments arranged by size:
4668 788 623 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2852 2996 3453 1186
Size: 1666 144 457 4097
Fragments arranged by size:
4097 1666 457 144
BseYI C'CCAG_C
Cuts at: 2138 2151 2361 2465 3175 3322 3328 3469 3631
Size: 13 210 104 710 147 6 141 162
Cuts at: 3631 5567 6063 2138
Size: 1936 496 2439
Fragments arranged by size:
2439 1936 710 496 210 162 147 141
104 13 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2957 3614 1163
Size: 573 1221 657 3913
Fragments arranged by size:
3913 1221 657 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 3814 4575 5603 1540
Size: 275 186 129 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186 129
BsiHKAI G_wGCw'C
Cuts at: 3381 3396 4425 4510 5581 3381
Size: 15 1029 85 1071 4164
Fragments arranged by size:
4164 1071 1029 85 15
BsmI GAATG_Cn'
Cuts at: 3261 3875 3968 3261
Size: 614 93 5657
Fragments arranged by size:
5657 614 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2139 2158 2246 2660 2692 3020 3258
Size: 399 349 19 88 414 32 328 238
Cuts at: 3258 3285 3301 3381 3396 3683 4425 4510 5581
Size: 27 16 80 15 287 742 85 1071
Cuts at: 5581 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 414 399 349 328 287
238 88 85 80 32 27 19 16
15
BspEI T'CCGG_A
Cuts at: 3048 6027 3048
Size: 2979 3385
BspHI T'CATG_A
Cuts at: 2643 4253 5983 2643
Size: 1610 1730 3024
Fragments arranged by size:
3024 1730 1610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2037 3123 3362 3719 844
Size: 1193 1086 239 357 3489
Fragments arranged by size:
3489 1193 1086 357 239
BsrBI CCG'CTC
Cuts at: 1394 1823 4251 1394
Size: 429 2428 3507
Fragments arranged by size:
3507 2428 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5008 5195 1700
Size: 110 3198 187 2869
Fragments arranged by size:
3198 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 2030 2414 4424 5436 2030
Size: 384 2010 1012 2958
Fragments arranged by size:
2958 2010 1012 384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 2398 2461 3277 2398
Size: 63 816 5485
Fragments arranged by size:
5485 816 63
BstYI r'GATC_y
Cuts at: 1972 3694 4061 4446 4463 5915 6001 6013 6359
Size: 1722 367 385 17 1452 86 12 346
Cuts at: 6359 1972
Size: 1977
Fragments arranged by size:
1977 1722 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2211 2955 2211
Size: 744 5620
BtgI C'CryG_G
Cuts at: 513 1065 2004 2313 2442 2631 2737 3433 3478
Size: 552 939 309 129 189 106 696 45
Cuts at: 3478 513
Size: 3399
Fragments arranged by size:
3399 939 696 552 309 189 129 106
45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5625
Fragments arranged by size:
5625 683 56
BtsI GCAGTG_nn'
Cuts at: 2760 2780 2940 3414 3539 3880 4674 4694 2760
Size: 20 160 474 125 341 794 20 4430
Fragments arranged by size:
4430 794 474 341 160 125 20 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3643 3678 529
Size: 35 3079 35 3215
Fragments arranged by size:
3215 3079 35 35
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DraIII CAC_nnn'GTG
Cuts at: 3069 3069
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5371 809
Size: 507 4055 1802
Fragments arranged by size:
4055 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2316 2400
Size: 54 1425 50 275 18 297 189 84
Cuts at: 2400 2734 3231 3267 3315 3534 3811 4700 6329
Size: 334 497 36 48 219 277 889 1629
Cuts at: 6329 8
Size: 43
Fragments arranged by size:
1629 1425 889 497 334 297 277 275
219 189 84 54 50 48 43 36
18
EagI C'GGCC_G
Cuts at: 1537 1812 2127 3811 1537
Size: 275 315 1684 4090
Fragments arranged by size:
4090 1684 315 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3386 4294 1353
Size: 2033 908 3423
Fragments arranged by size:
3423 2033 908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2267 4951 5325 5471 387
Size: 1064 138 678 2684 374 146 1280
Fragments arranged by size:
2684 1280 1064 678 374 146 138
EcoICRI GAG'CTC
Cuts at: 3379 3379
Size: 6364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2135 2347 2456 2531 2557 2710
Size: 393 413 90 212 109 75 26 153
Cuts at: 2710 4427 5024 5811 6079 1239
Size: 1717 597 787 268 1524
Fragments arranged by size:
1717 1524 787 597 413 393 268 212
153 109 90 75 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2506 2506
Size: 6364
EcoO109I rG'GnC_Cy
Cuts at: 2215 2243 2256 2887 3121 3452 2215
Size: 28 13 631 234 331 5127
Fragments arranged by size:
5127 631 331 234 28 13
EcoRI G'AATT_C
Cuts at: 3673 4051 3673
Size: 378 5986
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 3192 5511 1678
Size: 1514 2319 2531
Fragments arranged by size:
2531 2319 1514
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2056 2088 2191 2223 2245
Size: 32 135 32 484 32 103 32 22
Cuts at: 2245 2277 2847 2879 3429 3461 5005 5037 5079
Size: 32 570 32 550 32 1544 32 42
Cuts at: 5079 5111 1373
Size: 32 2626
Fragments arranged by size:
2626 1544 570 550 484 135 103 42
32 32 32 32 32 32 32 32
32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 3188 3188
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5478 5662 1305
Size: 4173 184 2007
Fragments arranged by size:
4173 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 3269 10
Size: 54 1425 343 570 867 3105
Fragments arranged by size:
3105 1425 867 570 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 3278 4322 4681 4840 518
Size: 648 1230 882 1044 359 159 2042
Fragments arranged by size:
2042 1230 1044 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2148 2325 2573 2750 2791 3571 3780 4457
Size: 542 177 248 177 41 780 209 677
Cuts at: 4457 5605 5850 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 780 677 542 248 245 209
177 177 41
NarI GG'CG_CC
Cuts at: 3189 3189
Size: 6364
NcoI C'CATG_G
Cuts at: 513 1065 2004 2442 2737 3478 513
Size: 552 939 438 295 741 3399
Fragments arranged by size:
3399 939 741 552 438 295
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 2113 3034 3722 5267 1132
Size: 97 884 921 688 1545 2229
Fragments arranged by size:
2229 1545 921 884 688 97
PasI CC'CwG_GG
Cuts at: 1348 3124 1348
Size: 1776 4588
PciI A'CATG_T
Cuts at: 5263 5263
Size: 6364
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2068 2091 2325 3292 1108
Size: 960 23 234 967 4180
Fragments arranged by size:
4180 967 960 234 23
PfoI T'CCnGG_A
Cuts at: 2022 2163 2022
Size: 141 6223
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6364
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2242 2688 2242
Size: 446 5918
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2591 2623 2591
Size: 32 6332
PstI C_TGCA'G
Cuts at: 830 2755 2790 2895 3120 3716 830
Size: 1925 35 105 225 596 3478
Fragments arranged by size:
3478 1925 596 225 105 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
PvuII CAG'CTG
Cuts at: 2325 2573 2750 2791 2325
Size: 248 177 41 5898
Fragments arranged by size:
5898 248 177 41
SacI G_AGCT'C
Cuts at: 3381 3381
Size: 6364
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SapI GCTCTTCn'nnn_
Cuts at: 3386 3386
Size: 6364
SbfI CC_TGCA'GG
Cuts at: 2895 3120 3716 2895
Size: 225 596 5543
Fragments arranged by size:
5543 596 225
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2751 2786 2891 3116 3367 3712 4847
Size: 219 1706 35 105 225 251 345 1135
Cuts at: 4847 5528 5719 826
Size: 681 191 1471
Fragments arranged by size:
1706 1471 1135 681 345 251 225 219
191 105 35
SfoI GGC'GCC
Cuts at: 3190 3190
Size: 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2203 4468 5369 5631 820
Size: 197 926 260 2265 901 262 1553
Fragments arranged by size:
2265 1553 926 901 262 260 197
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 2113 3034 3722 1132
Size: 981 921 688 3774
Fragments arranged by size:
3774 981 921 688
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2004 2194 2442 2697 2737 3312 3478
Size: 552 939 190 248 255 40 575 166
Cuts at: 3478 3803 513
Size: 325 3074
Fragments arranged by size:
3074 939 575 552 325 255 248 190
166 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2791 3461 4391 4408 4561 4746
Size: 1255 444 767 670 930 17 153 185
Cuts at: 4746 5191 325
Size: 445 1498
Fragments arranged by size:
1498 1255 930 767 670 445 444 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2598 2826 4610
Size: 274 80 33 51 1443 621 228 1784
Cuts at: 4610 6322 96
Size: 1712 138
Fragments arranged by size:
1784 1712 1443 621 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2844 2876 6324 6356 2844
Size: 32 3448 32 2852
Fragments arranged by size:
3448 2852 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6364
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 3155 2875
Size: 280 6084
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6364
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 4493 4493
Size: 6364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI
SexAI SfiI SrfI SwaI