Restriction map for
pFN21AE6482
(Circular) (Six-base) MAPSORT of: pFN21AE6482.seq Check: 6725 from: 1 to: 6319
pFN21AE6482 6319 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3119 3119
Size: 6319
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3303 4129 278
Size: 53 83 186 936 1767 826 2468
Fragments arranged by size:
2468 1767 936 826 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3662 1937
Size: 1725 4594
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2498 2630 3073 4382 5766 6034 1632
Size: 866 132 443 1309 1384 268 1917
Fragments arranged by size:
1917 1384 1309 866 443 268 132
AfeI AGC'GCT
Cuts at: 2799 2799
Size: 6319
AflII C'TTAA_G
Cuts at: 820 1017 3398 820
Size: 197 2381 3741
Fragments arranged by size:
3741 2381 197
AflIII A'CryG_T
Cuts at: 1203 2595 5218 6195 1203
Size: 1392 2623 977 1327
Fragments arranged by size:
2623 1392 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 3237 3340 3574 4062 5634 3237
Size: 103 234 488 1572 3922
Fragments arranged by size:
3922 1572 488 234 103
ApaI G_GGCC'C
Cuts at: 2199 2199
Size: 6319
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2533 3628 3854 4006 6115 1493
Size: 1040 1095 226 152 2109 1697
Fragments arranged by size:
2109 1697 1095 1040 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2307 2856 2904 3360 3593 3644 1870
Size: 73 364 549 48 456 233 51 4545
Fragments arranged by size:
4545 549 456 364 233 73 51 48
AvrII C'CTAG_G
Cuts at: 2405 2405
Size: 6319
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2455 2488 2455
Size: 33 6286
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 2828 2884 3365 3640
Size: 325 217 627 350 691 56 481 275
Cuts at: 3640 5095 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 691 627 481 350 325 275
217 56
BanII G_rGCy'C
Cuts at: 1391 2021 2199 2208 2819 3568 3638 1391
Size: 630 178 9 611 749 70 4072
Fragments arranged by size:
4072 749 630 611 178 70 9
BbeI G_GCGC'C
Cuts at: 3369 3369
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2736 2998 928
Size: 971 837 262 4249
Fragments arranged by size:
4249 971 837 262
BbvCI CC'TCA_GC
Cuts at: 2505 2505
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2629 2663 4510 4544 1903
Size: 34 692 34 1847 34 3678
Fragments arranged by size:
3678 1847 692 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2078 2203 2421
Size: 107 122 71 631 605 406 125 218
Cuts at: 2421 4930 136
Size: 2509 1525
Fragments arranged by size:
2509 1525 631 605 406 218 125 122
107 71
BglII A'GATC_T
Cuts at: 6314 6314
Size: 6319
BlpI GC'TnA_GC
Cuts at: 3494 3567 3736 3494
Size: 73 169 6077
Fragments arranged by size:
6077 169 73
Bme1580I G_kGCm'C
Cuts at: 1790 2142 2199 2642 3514 4380 5536 1790
Size: 352 57 443 872 866 1156 2573
Fragments arranged by size:
2573 1156 872 866 443 352 57
BmgBI CAC'GTC
Cuts at: 1318 3430 1318
Size: 2112 4207
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2193 3225 4050
Size: 416 53 83 399 24 776 1032 825
Cuts at: 4050 5008 442
Size: 958 1753
Fragments arranged by size:
1753 1032 958 825 776 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2040 2315 3079 3619 2040
Size: 275 764 540 4740
Fragments arranged by size:
4740 764 540 275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2112 2680 3074 4979 1239
Size: 873 568 394 1905 2579
Fragments arranged by size:
2579 1905 873 568 394
Bpu10I CC'TnA_GC
Cuts at: 2505 3047 2505
Size: 542 5777
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4444 5309 5607 4444
Size: 865 298 5156
Fragments arranged by size:
5156 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2341 3435 4982 882
Size: 617 842 1094 1547 2219
Fragments arranged by size:
2219 1547 1094 842 617
BsaAI yAC'GTr
Cuts at: 493 1204 3316 4110 493
Size: 711 2112 794 2702
Fragments arranged by size:
2702 2112 794 711
BsaBI GATnn'nnATC
Cuts at: 2783 6313 2783
Size: 3530 2789
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2099 3300 3366 4126
Size: 53 83 186 936 566 1201 66 760
Cuts at: 4126 4508 5143 275
Size: 382 635 1451
Fragments arranged by size:
1451 1201 936 760 635 566 382 186
83 66 53
BsaWI w'CCGG_w
Cuts at: 3303 4752 5150 5424 5571 5982 3303
Size: 1449 398 274 147 411 3640
Fragments arranged by size:
3640 1449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3434 3464 1178
Size: 30 165 30 2031 30 4033
Fragments arranged by size:
4033 2031 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2416 3176 3237 1186
Size: 880 350 760 61 4268
Fragments arranged by size:
4268 880 760 350 61
BseYI C'CCAG_C
Cuts at: 2260 2585 2618 2969 3118 3237 3333 3421 5522
Size: 325 33 351 149 119 96 88 2101
Cuts at: 5522 6018 2260
Size: 496 2561
Fragments arranged by size:
2561 2101 496 351 325 149 119 96
88 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2266 2972 1163
Size: 573 530 706 4510
Fragments arranged by size:
4510 706 573 530
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2646 3769 4530 5558 1540
Size: 275 186 645 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 645 275 186
BsiHKAI G_wGCw'C
Cuts at: 2021 2361 2819 2964 4380 4465 5536 2021
Size: 340 458 145 1416 85 1071 2804
Fragments arranged by size:
2804 1416 1071 458 340 145 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2142 2199 2208 2361 2642 2819
Size: 399 231 121 57 9 153 281 177
Cuts at: 2819 2964 3514 3568 3638 4380 4465 5536 1391
Size: 145 550 54 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 399 281 231 177
153 145 121 85 70 57 54 9
BspEI T'CCGG_A
Cuts at: 3303 5982 3303
Size: 2679 3640
BspHI T'CATG_A
Cuts at: 3141 4208 5938 3141
Size: 1067 1730 3522
Fragments arranged by size:
3522 1730 1067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2247 3119 3674 844
Size: 1403 872 555 3489
Fragments arranged by size:
3489 1403 872 555
BsrBI CCG'CTC
Cuts at: 1394 1823 4206 1394
Size: 429 2383 3507
Fragments arranged by size:
3507 2383 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 2294 2456 2887 3616 4963 5150
Size: 110 227 257 162 431 729 1347 187
Cuts at: 5150 1700
Size: 2869
Fragments arranged by size:
2869 1347 729 431 257 227 187 162
110
BsrGI T'GTAC_A
Cuts at: 96 2570 3469 96
Size: 2474 899 2946
Fragments arranged by size:
2946 2474 899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2959 3577 3613 794
Size: 2165 618 36 3500
Fragments arranged by size:
3500 2165 618 36
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 2125 2125
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 3532 3649 4016 4401 4418 5870 5956 5968
Size: 1560 117 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1560 1452 385 367 346 117 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2517 3030 513
Size: 552 939 513 513 3802
Fragments arranged by size:
3802 939 552 513 513
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 3835 4629 4649 3835
Size: 794 20 5505
Fragments arranged by size:
5505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2757 2792 529
Size: 35 2193 35 4056
Fragments arranged by size:
4056 2193 35 35
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DraIII CAC_nnn'GTG
Cuts at: 3274 3274
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2446 2889
Size: 54 1425 50 275 18 177 439 443
Cuts at: 2889 2988 3387 3766 4655 6284 8
Size: 99 399 379 889 1629 43
Fragments arranged by size:
1629 1425 889 443 439 399 379 275
177 99 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4249 1353
Size: 2896 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3068 4906 5280 5426 387
Size: 1064 138 1479 1838 374 146 1280
Fragments arranged by size:
1838 1479 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2019 2817 2019
Size: 798 5521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2112 2498 2630 2680 3073 3074 4382
Size: 393 480 386 132 50 393 1 1308
Cuts at: 4382 4979 5766 6034 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1308 787 597 480 393 393 386
268 132 50 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2219 2723 2048
Size: 171 504 5644
Fragments arranged by size:
5644 504 171
EcoO109I rG'GnC_Cy
Cuts at: 2087 2195 2212 2614 2715 2944 3227 2087
Size: 108 17 402 101 229 283 5179
Fragments arranged by size:
5179 402 283 229 108 101 17
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2496 2801 3369 5466 1678
Size: 818 305 568 2097 2531
Fragments arranged by size:
2531 2097 818 568 305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3434 3466 4960 4992 5034
Size: 32 135 32 1862 32 1494 32 42
Cuts at: 5034 5066 1373
Size: 32 2626
Fragments arranged by size:
2626 1862 1494 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 3365 3365
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5433 5617 1305
Size: 4128 184 2007
Fragments arranged by size:
4128 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2448 3389 10
Size: 54 1425 343 177 439 941 2940
Fragments arranged by size:
2940 1425 941 439 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4277 4636 4795 518
Size: 648 3111 359 159 2042
Fragments arranged by size:
3111 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2927 2956 2993 3337 3735 4412 5560 5805
Size: 1321 29 37 344 398 677 1148 245
Cuts at: 5805 1606
Size: 2120
Fragments arranged by size:
2120 1321 1148 677 398 344 245 37
29
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 6319
NarI GG'CG_CC
Cuts at: 3366 3366
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4828
Fragments arranged by size:
4828 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2599 3677 5222 1132
Size: 97 1370 1078 1545 2229
Fragments arranged by size:
2229 1545 1370 1078 97
PasI CC'CwG_GG
Cuts at: 1348 2221 2287 2919 3436 1348
Size: 873 66 632 517 4231
Fragments arranged by size:
4231 873 632 517 66
PciI A'CATG_T
Cuts at: 2595 5218 2595
Size: 2623 3696
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2851 3237 1108
Size: 1743 386 4190
Fragments arranged by size:
4190 1743 386
PfoI T'CCnGG_A
Cuts at: 2089 2089
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 3316 3316
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 2212 2614 2715 2087
Size: 125 402 101 5691
Fragments arranged by size:
5691 402 125 101
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspOMI G'GGCC_C
Cuts at: 2195 2195
Size: 6319
PspXI vC'TCGA_Gb
Cuts at: 3593 3593
Size: 6319
PstI C_TGCA'G
Cuts at: 830 2727 3671 830
Size: 1897 944 3478
Fragments arranged by size:
3478 1897 944
PvuI CG_AT'CG
Cuts at: 2001 2646 2001
Size: 645 5674
PvuII CAG'CTG
Cuts at: 2927 2956 3337 2927
Size: 29 381 5909
Fragments arranged by size:
5909 381 29
SacI G_AGCT'C
Cuts at: 2021 2819 2021
Size: 798 5521
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 3012 3270 3012
Size: 258 6061
SfcI C'TryA_G
Cuts at: 826 1045 2723 3667 4802 5483 5674 826
Size: 219 1678 944 1135 681 191 1471
Fragments arranged by size:
1678 1471 1135 944 681 219 191
SfoI GGC'GCC
Cuts at: 3367 3367
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 2309 2858 3362 3646 1872
Size: 437 549 504 284 4545
Fragments arranged by size:
4545 549 504 437 284
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3398 3593 4423 5324 5586 820
Size: 197 926 1455 195 830 901 262 1553
Fragments arranged by size:
1553 1455 926 901 830 262 197 195
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SrfI GCCC'GGGC
Cuts at: 2309 2309
Size: 6319
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 2410 3257 1517
Size: 893 847 4579
Fragments arranged by size:
4579 893 847
StyI C'CwwG_G
Cuts at: 513 1065 2004 2405 2892 3758 513
Size: 552 939 401 487 866 3074
Fragments arranged by size:
3074 939 866 552 487 401
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 2261 4346 4363 4516 4701 5146
Size: 1255 537 144 2085 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
2085 1498 1255 537 445 185 153 144
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2570 2929 3469
Size: 274 80 33 51 1443 593 359 540
Cuts at: 3469 4565 6277 96
Size: 1096 1712 138
Fragments arranged by size:
1712 1443 1096 593 540 359 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 3593 1943
Size: 1650 4669
XmaI C'CCGG_G
Cuts at: 1870 2307 2856 3360 3644 1870
Size: 437 549 504 284 4545
Fragments arranged by size:
4545 549 504 437 284
XmnI GAAnn'nnTTC
Cuts at: 4448 4448
Size: 6319
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3301 4127 276
Size: 53 83 186 936 1767 826 2468
Fragments arranged by size:
2468 1767 936 826 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV
FseI FspAI NruI NsiI PacI PsrI RsrII SacII
SanDI SapI SfiI SwaI XcmI