Restriction map for
pFN21AE6481
(Circular) (Six-base) MAPSORT of: pFN21AE6481.seq Check: 4841 from: 1 to: 6244
pFN21AE6481 6244 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2230 2230
Size: 6244
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2523 4054 278
Size: 53 83 186 936 987 1531 2468
Fragments arranged by size:
2468 1531 987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 4307 5691 5959 1632
Size: 1850 825 1384 268 1917
Fragments arranged by size:
1917 1850 1384 825 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 5143 6120 1203
Size: 3940 977 1327
Fragments arranged by size:
3940 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 4973 4973
Size: 6244
AleI CACnn'nnGTG
Cuts at: 1166 2115 1166
Size: 949 5295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 2052 3987 5559 2052
Size: 1935 1572 2737
Fragments arranged by size:
2737 1935 1572
ApaI G_GGCC'C
Cuts at: 2154 3386 2154
Size: 1232 5012
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 2324 2834 3344 3553 3779 3931 6040 1493
Size: 831 510 510 209 226 152 2109 1697
Fragments arranged by size:
2109 1697 831 510 510 226 209 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2483 3569 1870
Size: 73 127 413 1086 4545
Fragments arranged by size:
4545 1086 413 127 73
AvrII C'CTAG_G
Cuts at: 2059 2059
Size: 6244
BamHI G'GATC_C
Cuts at: 3023 3574 3941 3023
Size: 551 367 5326
Fragments arranged by size:
5326 551 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2258 2935 2943 3565 5020
Size: 325 217 627 471 677 8 622 1455
Cuts at: 5020 618
Size: 1842
Fragments arranged by size:
1842 1455 677 627 622 471 325 217
8
BanII G_rGCy'C
Cuts at: 1391 2040 2154 2577 3386 3563 1391
Size: 649 114 423 809 177 4072
Fragments arranged by size:
4072 809 649 423 177 114
BbeI G_GCGC'C
Cuts at: 2947 2947
Size: 6244
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2318 928
Size: 971 419 4854
Fragments arranged by size:
4854 971 419
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2035 3293 4855
Size: 107 122 71 631 605 363 1258 1562
Cuts at: 4855 136
Size: 1525
Fragments arranged by size:
1562 1525 1258 631 605 363 122 107
71
BglII A'GATC_T
Cuts at: 2601 3114 3147 6239 2601
Size: 513 33 3092 2606
Fragments arranged by size:
3092 2606 513 33
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 2154 3386 4305 5461 1790
Size: 364 1232 919 1156 2573
Fragments arranged by size:
2573 1232 1156 919 364
BmgBI CAC'GTC
Cuts at: 1318 3280 1318
Size: 1962 4282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2179 3975 4933
Size: 416 53 83 399 24 762 1796 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1796 1753 958 762 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3415 3415
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4904 1239
Size: 3665 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2267 4369 5234 5532 2267
Size: 2102 865 298 2979
Fragments arranged by size:
2979 2102 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2920 4907 882
Size: 617 1421 1987 2219
Fragments arranged by size:
2219 1987 1421 617
BsaAI yAC'GTr
Cuts at: 493 1204 3121 4035 493
Size: 711 1917 914 2702
Fragments arranged by size:
2702 1917 914 711
BsaBI GATnn'nnATC
Cuts at: 2555 3041 6238 2555
Size: 486 3197 2561
Fragments arranged by size:
3197 2561 486
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 2944 4051 4433
Size: 53 83 186 936 987 424 1107 382
Cuts at: 4433 5068 275
Size: 635 1451
Fragments arranged by size:
1451 1107 987 936 635 424 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2350 4677 5075 5349 5496 5907 2350
Size: 2327 398 274 147 411 2687
Fragments arranged by size:
2687 2327 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2453 2483 1178
Size: 30 165 30 1050 30 4939
Fragments arranged by size:
4939 1050 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2245 2501 2945 3323 1186
Size: 1059 256 444 378 4107
Fragments arranged by size:
4107 1059 444 378 256
BseYI C'CCAG_C
Cuts at: 2368 5447 5943 2368
Size: 3079 496 2669
Fragments arranged by size:
3079 2669 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2085 2158 3302 1163
Size: 573 349 73 1144 4105
Fragments arranged by size:
4105 1144 573 349 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2793 3694 4455 5483 1540
Size: 275 186 792 901 761 1028 2301
Fragments arranged by size:
2301 1028 901 792 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3171 4305 4390 5461 3171
Size: 1134 85 1071 3954
Fragments arranged by size:
3954 1134 1071 85
BsmI GAATG_Cn'
Cuts at: 3468 3755 3848 3468
Size: 287 93 5864
Fragments arranged by size:
5864 287 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2154 2577 3171 3386 3563 4305
Size: 399 250 114 423 594 215 177 742
Cuts at: 4305 4390 5461 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 594 423 399 250 215
177 114 85
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 2523 4133 5863 2523
Size: 1610 1730 2904
Fragments arranged by size:
2904 1730 1610
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2230 3339 3599 844
Size: 1386 1109 260 3489
Fragments arranged by size:
3489 1386 1109 260
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 2597 2797 2995 3399 4131 1394
Size: 429 412 362 200 198 404 732 3507
Fragments arranged by size:
3507 732 429 412 404 362 200 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 2532 4739 4913 65
Size: 1183 1284 2207 174 1396
Fragments arranged by size:
2207 1396 1284 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4888 5075 1700
Size: 110 3078 187 2869
Fragments arranged by size:
3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2967 96
Size: 2871 3373
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2052 794
Size: 1258 4986
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 2089 1184
Size: 905 5339
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 2550 2601 3023 3036 3114 3147 3574 3941
Size: 578 51 422 13 78 33 427 367
Cuts at: 3941 4326 4343 5795 5881 5893 6239 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 578 427 422 385 367 346
86 78 51 33 17 13 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2010 2539 2685 2745 3229 513
Size: 552 939 6 529 146 60 484 3528
Fragments arranged by size:
3528 939 552 529 484 146 60 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 2146 2826 3534 3760 4554 4574 2146
Size: 680 708 226 794 20 3816
Fragments arranged by size:
3816 794 708 680 226 20
ClaI AT'CG_AT
Cuts at: 1554 2301 1554
Size: 747 5497
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5251 809
Size: 507 3935 1802
Fragments arranged by size:
3935 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2449 2542
Size: 54 1425 50 275 18 177 442 93
Cuts at: 2542 2775 2979 3691 4580 6209 8
Size: 233 204 712 889 1629 43
Fragments arranged by size:
1629 1425 889 712 442 275 233 204
177 93 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2768 4174 1353
Size: 1415 1406 3423
Fragments arranged by size:
3423 1415 1406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2441 4831 5205 5351 387
Size: 1064 138 852 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 852 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3482 4307 4904 5691 5959 1239
Size: 393 1850 825 597 787 268 1524
Fragments arranged by size:
1850 1524 825 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2151 3328 3382 3383 3520 2151
Size: 1177 54 1 137 4875
Fragments arranged by size:
4875 1177 137 54 1
EcoRI G'AATT_C
Cuts at: 2834 3344 3553 3931 2834
Size: 510 209 378 5147
Fragments arranged by size:
5147 510 378 209
EcoRV GAT'ATC
Cuts at: 2443 3067 2443
Size: 624 5620
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2743 2743
Size: 6244
FspI TGC'GCA
Cuts at: 3440 4750 3440
Size: 1310 4934
HaeII r_GCGC'y
Cuts at: 1678 2822 2947 3317 3515 5391 1678
Size: 1144 125 370 198 1876 2531
Fragments arranged by size:
2531 1876 1144 370 198 125
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2581 2613 3012 3044 4885
Size: 32 135 32 1009 32 399 32 1841
Cuts at: 4885 4917 4959 4991 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1841 1009 399 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KasI G'GCGC_C
Cuts at: 2943 2943
Size: 6244
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3267 5358 5542 1305
Size: 1962 2091 184 2007
Fragments arranged by size:
2091 2007 1962 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2451 2777 2981 10
Size: 54 1425 343 177 442 326 204 3273
Fragments arranged by size:
3273 1425 442 343 326 204 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2115 2690 3164 3234 4202 4561
Size: 648 843 106 575 474 70 968 359
Cuts at: 4561 4720 518
Size: 159 2042
Fragments arranged by size:
2042 968 843 648 575 474 359 159
106 70
MspA1I CmG'CkG
Cuts at: 1606 2541 3250 3660 4337 5485 5730 1606
Size: 935 709 410 677 1148 245 2120
Fragments arranged by size:
2120 1148 935 709 677 410 245
NarI GG'CG_CC
Cuts at: 2944 2944
Size: 6244
NcoI C'CATG_G
Cuts at: 513 1065 2004 2010 2685 2745 3229 513
Size: 552 939 6 675 60 484 3528
Fragments arranged by size:
3528 939 675 552 484 60 6
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 2083 2368 2864 3602 5147 1132
Size: 97 854 285 496 738 1545 2229
Fragments arranged by size:
2229 1545 854 738 496 285 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6244
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6244
PfoI T'CCnGG_A
Cuts at: 2956 2956
Size: 6244
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3328 3520 3328
Size: 192 6052
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspOMI G'GGCC_C
Cuts at: 2150 3382 2150
Size: 1232 5012
PstI C_TGCA'G
Cuts at: 830 3354 3519 3596 830
Size: 2524 165 77 3478
Fragments arranged by size:
3478 2524 165 77
PvuI CG_AT'CG
Cuts at: 2001 2793 2001
Size: 792 5452
PvuII CAG'CTG
Cuts at: 3250 3250
Size: 6244
SacII CC_GC'GG
Cuts at: 2542 2542
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SfcI C'TryA_G
Cuts at: 826 1045 2125 3350 3515 3592 4727 5408 5599
Size: 219 1080 1225 165 77 1135 681 191
Cuts at: 5599 826
Size: 1471
Fragments arranged by size:
1471 1225 1135 1080 681 219 191 165
77
SfoI GGC'GCC
Cuts at: 2945 2945
Size: 6244
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 4348 5249 5511 820
Size: 197 926 303 2102 901 262 1553
Fragments arranged by size:
2102 1553 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 2029 2903 1517
Size: 512 874 4858
Fragments arranged by size:
4858 874 512
StyI C'CwwG_G
Cuts at: 513 1065 2004 2010 2059 2262 2634 2685 2745
Size: 552 939 6 49 203 372 51 60
Cuts at: 2745 3229 3450 3683 513
Size: 484 221 233 3074
Fragments arranged by size:
3074 939 552 484 372 233 221 203
60 51 49 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2123 3086 4271 4288 4441 4626 5071
Size: 1255 543 963 1185 17 153 185 445
Cuts at: 5071 325
Size: 1498
Fragments arranged by size:
1498 1255 1185 963 543 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 4490 6202
Size: 274 80 33 51 1443 990 1523 1712
Cuts at: 6202 96
Size: 138
Fragments arranged by size:
1712 1523 1443 990 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6204 6236 6204
Size: 32 6212
Tth111I GACn'n_nGTC
Cuts at: 1316 2171 1316
Size: 855 5389
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2641 2641
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 3368 4373 3368
Size: 1005 5239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2521 4052 276
Size: 53 83 186 936 987 1531 2468
Fragments arranged by size:
2468 1531 987 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI