Restriction map for
pFN21AE6480
(Circular) (Six-base) MAPSORT of: pFN21AE6480.seq Check: 1751 from: 1 to: 6235
pFN21AE6480 6235 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3074 4045 278
Size: 53 83 186 936 1538 971 2468
Fragments arranged by size:
2468 1538 971 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 3578 1937
Size: 72 1569 4594
Fragments arranged by size:
4594 1569 72
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2090 2561 2873 3312 4298 5682 5950 1632
Size: 458 471 312 439 986 1384 268 1917
Fragments arranged by size:
1917 1384 986 471 458 439 312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 2832 3213 3529 5134 6111 1203
Size: 1629 381 316 1605 977 1327
Fragments arranged by size:
1629 1605 1327 977 381 316
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 2711 3978 5550 2711
Size: 1267 1572 3396
Fragments arranged by size:
3396 1572 1267
ApaI G_GGCC'C
Cuts at: 2267 2267
Size: 6235
ApaLI G'TGCA_C
Cuts at: 2132 3525 4292 5448 2132
Size: 1393 767 1156 2919
Fragments arranged by size:
2919 1393 1156 767
ApoI r'AATT_y
Cuts at: 1493 2195 3544 3770 3922 6031 1493
Size: 702 1349 226 152 2109 1697
Fragments arranged by size:
2109 1697 1349 702 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2762 3560 1870
Size: 73 819 798 4545
Fragments arranged by size:
4545 819 798 73
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2591 3556 5011 618
Size: 325 217 627 511 293 965 1455 1842
Fragments arranged by size:
1842 1455 965 627 511 325 293 217
BanII G_rGCy'C
Cuts at: 1391 2153 2267 2328 2763 3554 1391
Size: 762 114 61 435 791 4072
Fragments arranged by size:
4072 791 762 435 114 61
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2861 3148 3300 928
Size: 971 962 287 152 3863
Fragments arranged by size:
3863 971 962 287 152
BbvCI CC'TCA_GC
Cuts at: 2237 2492 2237
Size: 255 5980
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6230 6230
Size: 6235
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2136 2267 2807 2823 2842 3529 4296 5452
Size: 346 131 540 16 19 687 767 1156
Cuts at: 5452 1790
Size: 2573
Fragments arranged by size:
2573 1156 767 687 540 346 131 19
16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3966 4924 442
Size: 416 53 83 399 24 2549 958 1753
Fragments arranged by size:
2549 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2587 4895 1239
Size: 1348 2308 2579
Fragments arranged by size:
2579 2308 1348
Bpu10I CC'TnA_GC
Cuts at: 2237 2492 2237
Size: 255 5980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4360 5225 5523 4360
Size: 865 298 5072
Fragments arranged by size:
5072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 4898 882
Size: 617 1269 2130 2219
Fragments arranged by size:
2219 2130 1269 617
BsaAI yAC'GTr
Cuts at: 493 1204 3216 4026 493
Size: 711 2012 810 2702
Fragments arranged by size:
2702 2012 810 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3071 3117 4042 4424
Size: 53 83 186 936 1538 46 925 382
Cuts at: 4424 5059 275
Size: 635 1451
Fragments arranged by size:
1538 1451 936 925 635 382 186 83
53 46
BsaWI w'CCGG_w
Cuts at: 2507 2844 4668 5066 5340 5487 5898 2507
Size: 337 1824 398 274 147 411 2844
Fragments arranged by size:
2844 1824 411 398 337 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2313 2343 1178
Size: 30 165 30 910 30 5070
Fragments arranged by size:
5070 910 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2110 2786 1186
Size: 924 676 4635
Fragments arranged by size:
4635 924 676
BseYI C'CCAG_C
Cuts at: 2343 3367 5438 5934 2343
Size: 1024 2071 496 2644
Fragments arranged by size:
2644 2071 1024 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5662
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3685 4446 5474 1540
Size: 275 186 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 2136 3529 4296 4381 5452 2118
Size: 18 1393 767 85 1071 2901
Fragments arranged by size:
2901 1393 1071 767 85 18
BsiWI C'GTAC_G
Cuts at: 3216 3216
Size: 6235
BsmI GAATG_Cn'
Cuts at: 2578 3483 3746 3839 2578
Size: 905 263 93 4974
Fragments arranged by size:
4974 905 263 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2464 2464
Size: 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2136 2153 2267 2328 2763 2807
Size: 399 328 18 17 114 61 435 44
Cuts at: 2807 2823 2842 3529 3554 4296 4381 5452 1391
Size: 16 19 687 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 687 435 399 328 114
85 61 44 25 19 18 17 16
BspEI T'CCGG_A
Cuts at: 2844 5898 2844
Size: 3054 3181
BspHI T'CATG_A
Cuts at: 2017 2628 4124 5854 2017
Size: 611 1496 1730 2398
Fragments arranged by size:
2398 1730 1496 611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2439 2445 2796 3590 844
Size: 1595 6 351 794 3489
Fragments arranged by size:
3489 1595 794 351 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4122 1394
Size: 429 2299 3507
Fragments arranged by size:
3507 2299 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3362 4730 4904 65
Size: 1183 2114 1368 174 1396
Fragments arranged by size:
2114 1396 1368 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4879 5066 1700
Size: 110 3069 187 2869
Fragments arranged by size:
3069 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6235
BstBI TT'CG_AA
Cuts at: 3487 5039 3487
Size: 1552 4683
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 3390 3565 3932 4317 4334 5786 5872 5884
Size: 1418 175 367 385 17 1452 86 12
Cuts at: 5884 6230 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1418 385 367 346 175 86
17 12
BstZ17I GTA'TAC
Cuts at: 2010 2010
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 2209 2499 513
Size: 552 1144 290 4249
Fragments arranged by size:
4249 1144 552 290
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 3751 4545 4565 3751
Size: 794 20 5421
Fragments arranged by size:
5421 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3165 3318 3682
Size: 54 1425 50 275 18 1335 153 364
Cuts at: 3682 4571 6200 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1335 889 364 275 153 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 2462 2739 4165 1353
Size: 1034 75 277 1426 3423
Fragments arranged by size:
3423 1426 1034 277 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2109 4822 5196 5342 387
Size: 1064 138 520 2713 374 146 1280
Fragments arranged by size:
2713 1280 1064 520 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2090 2561 2587 2873 3312 4298 4895
Size: 393 458 471 26 286 439 986 597
Cuts at: 4895 5682 5950 1239
Size: 787 268 1524
Fragments arranged by size:
1524 986 787 597 471 458 439 393
286 268 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2483 3381 2483
Size: 898 5337
EcoO109I rG'GnC_Cy
Cuts at: 2264 2488 2634 2264
Size: 224 146 5865
Fragments arranged by size:
5865 224 146
EcoRI G'AATT_C
Cuts at: 2195 3544 3922 2195
Size: 1349 378 4508
Fragments arranged by size:
4508 1349 378
FspI TGC'GCA
Cuts at: 2554 4741 2554
Size: 2187 4048
HaeII r_GCGC'y
Cuts at: 1678 2627 5382 1678
Size: 949 2755 2531
Fragments arranged by size:
2755 2531 949
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2313 2345 2463 2495 2914
Size: 32 135 32 741 32 118 32 419
Cuts at: 2914 2946 4876 4908 4950 4982 1373
Size: 32 1930 32 42 32 2626
Fragments arranged by size:
2626 1930 741 419 135 118 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2332 2721 3579 3825 669
Size: 988 281 394 389 858 246 3079
Fragments arranged by size:
3079 988 858 394 389 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5349 5533 1305
Size: 4044 184 2007
Fragments arranged by size:
4044 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3167 3320 10
Size: 54 1425 343 1335 153 2925
Fragments arranged by size:
2925 1425 1335 343 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4193 4552 4711 518
Size: 648 3027 359 159 2042
Fragments arranged by size:
3027 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2211 2405 2418 2438 2865 3651 4328 5476
Size: 605 194 13 20 427 786 677 1148
Cuts at: 5476 5721 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 786 677 605 427 245 194
20 13
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5683
NdeI CA'TA_TG
Cuts at: 387 3431 387
Size: 3044 3191
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 2836 3533 3593 5138 1132
Size: 97 1607 697 60 1545 2229
Fragments arranged by size:
2229 1607 1545 697 97 60
PasI CC'CwG_GG
Cuts at: 1348 2164 2338 2485 1348
Size: 816 174 147 5098
Fragments arranged by size:
5098 816 174 147
PciI A'CATG_T
Cuts at: 2832 3529 5134 2832
Size: 697 1605 3933
Fragments arranged by size:
3933 1605 697
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6235
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 2263 2263
Size: 6235
PstI C_TGCA'G
Cuts at: 830 3587 830
Size: 2757 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2405 2865 2405
Size: 460 5775
SacII CC_GC'GG
Cuts at: 2212 2212
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SfcI C'TryA_G
Cuts at: 826 1045 3583 4718 5399 5590 826
Size: 219 2538 1135 681 191 1471
Fragments arranged by size:
2538 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4339 5240 5502 820
Size: 197 926 2396 901 262 1553
Fragments arranged by size:
2396 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 2587 3674 513
Size: 552 1522 1087 3074
Fragments arranged by size:
3074 1522 1087 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2094 2156 2286 2335 4262 4279 4432
Size: 1255 514 62 130 49 1927 17 153
Cuts at: 4432 4617 5062 325
Size: 185 445 1498
Fragments arranged by size:
1927 1498 1255 514 445 185 153 130
62 49 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4481 6193 96
Size: 274 80 33 51 1443 2504 1712 138
Fragments arranged by size:
2504 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6195 6227 6195
Size: 32 6203
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 2891 3155 4364 2891
Size: 264 1209 4762
Fragments arranged by size:
4762 1209 264
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3072 4043 276
Size: 53 83 186 936 1538 971 2468
Fragments arranged by size:
2468 1538 971 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BplI BstXI Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI