Restriction map for
pFN21AE6479
(Circular) (Six-base) MAPSORT of: pFN21AE6479.seq Check: 4086 from: 1 to: 6130
pFN21AE6479 6130 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 2934 3451 2934
Size: 517 5613
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3075 3154 3443 4193 5577 5845 1632
Size: 1443 79 289 750 1384 268 1917
Fragments arranged by size:
1917 1443 1384 750 289 268 79
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 5029 6006 1203
Size: 3826 977 1327
Fragments arranged by size:
3826 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 2513 3873 5445 2513
Size: 1360 1572 3198
Fragments arranged by size:
3198 1572 1360
ApaI G_GGCC'C
Cuts at: 2411 2411
Size: 6130
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 3455 1870
Size: 73 1512 4545
Fragments arranged by size:
4545 1512 73
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2298 2934 2966 3451 4906
Size: 325 217 627 511 636 32 485 1455
Cuts at: 4906 618
Size: 1842
Fragments arranged by size:
1842 1455 636 627 511 485 325 217
32
BanII G_rGCy'C
Cuts at: 1391 2177 2264 2349 2411 2708 2750 3337 3449
Size: 786 87 85 62 297 42 587 112
Cuts at: 3449 1391
Size: 4072
Fragments arranged by size:
4072 786 587 297 112 87 85 62
42
BbeI G_GCGC'C
Cuts at: 2970 2970
Size: 6130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2285 3215 928
Size: 971 386 930 3843
Fragments arranged by size:
3843 971 930 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2493 2637
Size: 107 122 71 631 605 337 484 144
Cuts at: 2637 4741 136
Size: 2104 1525
Fragments arranged by size:
2104 1525 631 605 484 337 144 122
107 71
BglII A'GATC_T
Cuts at: 6125 6125
Size: 6130
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2301 2411 4191 5347 1790
Size: 511 110 1780 1156 2573
Fragments arranged by size:
2573 1780 1156 511 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6130
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 4790 1239
Size: 1106 2445 2579
Fragments arranged by size:
2579 2445 1106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4255 5120 5418 4255
Size: 865 298 4967
Fragments arranged by size:
4967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3166 3418 4793 882
Size: 617 1667 252 1375 2219
Fragments arranged by size:
2219 1667 1375 617 252
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 2043 6124 2043
Size: 4081 2049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2967 3937 4319 4954
Size: 53 83 186 936 1434 970 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1451 1434 970 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4563 4961 5235 5382 5793 4563
Size: 398 274 147 411 4900
Fragments arranged by size:
4900 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2193 2223 1178
Size: 30 165 30 790 30 5085
Fragments arranged by size:
5085 790 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2834 2873 1186
Size: 1330 318 39 4443
Fragments arranged by size:
4443 1330 318 39
BseYI C'CCAG_C
Cuts at: 2257 2334 2599 3191 3417 5333 5829 2257
Size: 77 265 592 226 1916 496 2558
Fragments arranged by size:
2558 1916 592 496 265 226 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 2689 2879 1163
Size: 573 594 359 190 4414
Fragments arranged by size:
4414 594 573 359 190
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2826 4191 4276 5347 2826
Size: 1365 85 1071 3609
Fragments arranged by size:
3609 1365 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
BsmBI CGTCTCn'nnnn_
Cuts at: 2361 2946 2361
Size: 585 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2264 2301 2349 2411 2708 2750
Size: 399 387 87 37 48 62 297 42
Cuts at: 2750 2826 3337 3449 4191 4276 5347 1391
Size: 76 511 112 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 511 399 387 297 112
87 85 76 62 48 42 37
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 4019 5749 4019
Size: 1730 4400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3061 3485 844
Size: 2217 424 3489
Fragments arranged by size:
3489 2217 424
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 4017 1394
Size: 429 410 1784 3507
Fragments arranged by size:
3507 1784 429 410
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2417 4774 4961 1700
Size: 110 607 2357 187 2869
Fragments arranged by size:
2869 2357 607 187 110
BsrGI T'GTAC_A
Cuts at: 96 3132 96
Size: 3036 3094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 3035 4190 5202 3035
Size: 1155 1012 3963
Fragments arranged by size:
3963 1155 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2875 3010 3391 2875
Size: 135 381 5614
Fragments arranged by size:
5614 381 135
BstYI r'GATC_y
Cuts at: 1972 2226 2886 3460 3827 4212 4229 5681 5767
Size: 254 660 574 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 660 574 385 367 346 254
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2633 2633
Size: 6130
BtgI C'CryG_G
Cuts at: 513 1065 2004 2799 2953 3142 3285 513
Size: 552 939 795 154 189 143 3358
Fragments arranged by size:
3358 939 795 552 189 154 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 3646 4440 4460 3646
Size: 794 20 5316
Fragments arranged by size:
5316 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2061 2096 529
Size: 35 1497 35 4563
Fragments arranged by size:
4563 1497 35 35
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2105 2415 2802
Size: 54 1425 50 275 18 275 310 387
Cuts at: 2802 3393 3577 4466 6095 8
Size: 591 184 889 1629 43
Fragments arranged by size:
1629 1425 889 591 387 310 275 275
184 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2811 3193 4060 1353
Size: 755 703 382 867 3423
Fragments arranged by size:
3423 867 755 703 382
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4717 5091 5237 387
Size: 1064 138 3128 374 146 1280
Fragments arranged by size:
3128 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 3075 3154 3443 4193 4790 5577
Size: 393 713 730 79 289 750 597 787
Cuts at: 5577 5845 1239
Size: 268 1524
Fragments arranged by size:
1524 787 750 730 713 597 393 289
268 79
EcoNI CCTnn'n_nnAGG
Cuts at: 3407 3407
Size: 6130
EcoO109I rG'GnC_Cy
Cuts at: 2407 2518 2407
Size: 111 6019
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
EcoRV GAT'ATC
Cuts at: 2194 2194
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2970 5277 1678
Size: 1292 2307 2531
Fragments arranged by size:
2531 2307 1292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 2472 2504 4771
Size: 32 135 32 537 32 331 32 2267
Cuts at: 4771 4803 4845 4877 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2267 537 331 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2473 3474 3720 669
Size: 988 281 535 1001 246 3079
Fragments arranged by size:
3079 1001 988 535 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KasI G'GCGC_C
Cuts at: 2966 2966
Size: 6130
KpnI G_GTAC'C
Cuts at: 2938 3455 2938
Size: 517 5613
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2289 3189 5244 5428 1305
Size: 984 900 2055 184 2007
Fragments arranged by size:
2055 2007 984 900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2304 2357 2660 2918 4088 4447 4606
Size: 648 1138 53 303 258 1170 359 159
Cuts at: 4606 518
Size: 2042
Fragments arranged by size:
2042 1170 1138 648 359 303 258 159
53
MspA1I CmG'CkG
Cuts at: 1606 3323 3421 3546 4223 5371 5616 1606
Size: 1717 98 125 677 1148 245 2120
Fragments arranged by size:
2120 1717 1148 677 245 125 98
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 6130
NarI GG'CG_CC
Cuts at: 2967 2967
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 2004 2953 513
Size: 552 939 949 3690
Fragments arranged by size:
3690 949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 3488 5033 1132
Size: 97 2259 1545 2229
Fragments arranged by size:
2259 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3156 1348
Size: 1808 4322
PciI A'CATG_T
Cuts at: 5029 5029
Size: 6130
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3171 1613
Size: 1558 4572
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PspOMI G'GGCC_C
Cuts at: 2407 2407
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2155 2320 3482 830
Size: 1325 165 1162 3478
Fragments arranged by size:
3478 1325 1162 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 3421 3421
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 3193 3193
Size: 6130
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2151 2316 3478 4613 5294 5485 826
Size: 219 1106 165 1162 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 1106 681 219 191 165
SfoI GGC'GCC
Cuts at: 2968 2968
Size: 6130
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 3457 1872
Size: 1585 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4234 5135 5397 820
Size: 197 926 2291 901 262 1553
Fragments arranged by size:
2291 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2004 2868 2953 3569 513
Size: 552 939 864 85 616 3074
Fragments arranged by size:
3074 939 864 616 552 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2068 4157 4174 4327 4512 4957 325
Size: 1255 488 2089 17 153 185 445 1498
Fragments arranged by size:
2089 1498 1255 488 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2031 3132 4376
Size: 274 80 33 51 1443 54 1101 1244
Cuts at: 4376 6088 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1244 1101 274 138 80 54
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6090 6122 6090
Size: 32 6098
Tth111I GACn'n_nGTC
Cuts at: 1316 2573 1316
Size: 1257 4873
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 3455 1870
Size: 1585 4545
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI
XcmI