Restriction map for
pFN21AE6477
(Circular) (Six-base) MAPSORT of: pFN21AE6477.seq Check: 9544 from: 1 to: 6220
pFN21AE6477 6220 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3417 4030 278
Size: 53 83 186 936 1881 613 2468
Fragments arranged by size:
2468 1881 936 613 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3014 3447 3563 1937
Size: 1077 433 116 4594
Fragments arranged by size:
4594 1077 433 116
Acc65I G'GTAC_C
Cuts at: 3541 3541
Size: 6220
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3290 4283 5667 5935 1632
Size: 1658 993 1384 268 1917
Fragments arranged by size:
1917 1658 1384 993 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2115 5119 6096 1203
Size: 912 3004 977 1327
Fragments arranged by size:
3004 1327 977 912
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 2570 2736 2988 1166
Size: 1404 166 252 4398
Fragments arranged by size:
4398 1404 252 166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2048 2080 2048
Size: 32 6188
AlwNI CAG_nnn'CTG
Cuts at: 2787 3963 5535 2787
Size: 1176 1572 3472
Fragments arranged by size:
3472 1572 1176
ApaI G_GGCC'C
Cuts at: 2233 2233
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 2557 3529 3755 3907 6016 1493
Size: 1064 972 226 152 2109 1697
Fragments arranged by size:
2109 1697 1064 972 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 3545 1870
Size: 73 1602 4545
Fragments arranged by size:
4545 1602 73
BamHI G'GATC_C
Cuts at: 2883 3550 3917 2883
Size: 667 367 5186
Fragments arranged by size:
5186 667 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3317 3541 4996 618
Size: 325 217 627 1530 224 1455 1842
Fragments arranged by size:
1842 1530 1455 627 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2233 2433 2591 3063 3363 3539 1391
Size: 842 200 158 472 300 176 4072
Fragments arranged by size:
4072 842 472 300 200 176 158
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3382 928
Size: 971 1483 3766
Fragments arranged by size:
3766 1483 971
BbvCI CC'TCA_GC
Cuts at: 2727 2727
Size: 6220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2500 2534 2834 2868 4411
Size: 34 50 34 479 34 300 34 1543
Cuts at: 4411 4445 1903
Size: 34 3678
Fragments arranged by size:
3678 1543 479 300 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 3186 1548
Size: 1638 4582
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2978 2999 3273
Size: 107 122 71 631 605 1306 21 274
Cuts at: 3273 4831 136
Size: 1558 1525
Fragments arranged by size:
1558 1525 1306 631 605 274 122 107
71 21
BglII A'GATC_T
Cuts at: 3456 6215 3456
Size: 2759 3461
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2233 4281 5437 1790
Size: 443 2048 1156 2573
Fragments arranged by size:
2573 2048 1156 443
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6220
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3951 4909 442
Size: 416 53 83 399 24 2534 958 1753
Fragments arranged by size:
2534 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2035 4880 1239
Size: 796 2845 2579
Fragments arranged by size:
2845 2579 796
Bpu10I CC'TnA_GC
Cuts at: 2727 3479 2727
Size: 752 5468
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2831 4345 5210 5508 2831
Size: 1514 865 298 3543
Fragments arranged by size:
3543 1514 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4883 882
Size: 617 3384 2219
Fragments arranged by size:
3384 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4011 493
Size: 711 2807 2702
Fragments arranged by size:
2807 2702 711
BsaBI GATnn'nnATC
Cuts at: 3185 6214 3185
Size: 3029 3191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2670 3414 4027 4409
Size: 53 83 186 936 1137 744 613 382
Cuts at: 4409 5044 275
Size: 635 1451
Fragments arranged by size:
1451 1137 936 744 635 613 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2880 4653 5051 5325 5472 5883 2880
Size: 1773 398 274 147 411 3217
Fragments arranged by size:
3217 1773 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2715 1186
Size: 1529 4691
BseYI C'CCAG_C
Cuts at: 2226 3002 3356 5423 5919 2226
Size: 776 354 2067 496 2527
Fragments arranged by size:
2527 2067 776 496 354
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2138 2242 1163
Size: 573 402 104 5141
Fragments arranged by size:
5141 573 402 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3670 4431 5459 1540
Size: 275 186 1669 761 1028 2301
Fragments arranged by size:
2301 1669 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2433 4281 4366 5437 2433
Size: 1848 85 1071 3216
Fragments arranged by size:
3216 1848 1071 85
BsmI GAATG_Cn'
Cuts at: 2373 3731 3824 2373
Size: 1358 93 4769
Fragments arranged by size:
4769 1358 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3070 3070
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2233 2433 2591 3063 3363 3539 4281
Size: 399 443 200 158 472 300 176 742
Cuts at: 4281 4366 5437 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 472 443 399 300 200
176 158 85
BspEI T'CCGG_A
Cuts at: 2880 5883 2880
Size: 3003 3217
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3575 844
Size: 2731 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2335 2344 2805 4107 1394
Size: 429 512 9 461 1302 3507
Fragments arranged by size:
3507 1302 512 461 429 9
BsrDI GCAATG_nn'
Cuts at: 65 1248 4715 4889 65
Size: 1183 3467 174 1396
Fragments arranged by size:
3467 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2600 3320 3438 4864 5051 1700
Size: 110 790 720 118 1426 187 2869
Fragments arranged by size:
2869 1426 790 720 187 118 110
BsrGI T'GTAC_A
Cuts at: 96 3138 96
Size: 3042 3178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 2426 4280 5292 2426
Size: 1854 1012 3354
Fragments arranged by size:
3354 1854 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 2497 1184
Size: 1313 4907
BstYI r'GATC_y
Cuts at: 1972 2883 3456 3550 3917 4302 4319 5771 5857
Size: 911 573 94 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 911 573 385 367 346 94
86 17 12
BstZ17I GTA'TAC
Cuts at: 3448 3448
Size: 6220
Bsu36I CC'TnA_GG
Cuts at: 2766 2766
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2734 3294 3372 513
Size: 552 1669 560 78 3361
Fragments arranged by size:
3361 1669 560 552 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2275 2953 487
Size: 683 56 1049 678 3754
Fragments arranged by size:
3754 1049 683 678 56
BtsI GCAGTG_nn'
Cuts at: 2627 3231 3736 4530 4550 2627
Size: 604 505 794 20 4297
Fragments arranged by size:
4297 794 604 505 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 2217 2217
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2820 3151 3303
Size: 54 1425 50 275 18 990 331 152
Cuts at: 3303 3667 4556 6185 8
Size: 364 889 1629 43
Fragments arranged by size:
1629 1425 990 889 364 331 275 152
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3667 1537
Size: 275 1855 4090
Fragments arranged by size:
4090 1855 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2690 2841 3026 4150 1353
Size: 1337 151 185 1124 3423
Fragments arranged by size:
3423 1337 1124 185 151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4807 5181 5327 387
Size: 1064 138 3218 374 146 1280
Fragments arranged by size:
3218 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2431 2431
Size: 6220
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 3290 4283 4880 5667 5935 1239
Size: 393 403 1255 993 597 787 268 1524
Fragments arranged by size:
1524 1255 993 787 597 403 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2050 2050
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2230 2626 2770 3007 3199 3344 2230
Size: 396 144 237 192 145 5106
Fragments arranged by size:
5106 396 237 192 145 144
EcoRI G'AATT_C
Cuts at: 2557 3529 3907 2557
Size: 972 378 4870
Fragments arranged by size:
4870 972 378
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2879 5367 1678
Size: 1201 2488 2531
Fragments arranged by size:
2531 2488 1201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2061 2093 4861 4893 4935
Size: 32 135 32 489 32 2768 32 42
Cuts at: 4935 4967 1373
Size: 32 2626
Fragments arranged by size:
2768 2626 489 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KpnI G_GTAC'C
Cuts at: 3545 3545
Size: 6220
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2822 3153 10
Size: 54 1425 343 990 331 3077
Fragments arranged by size:
3077 1425 990 343 331 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2570 2609 2736 2988 3179 4178
Size: 648 1140 264 39 127 252 191 999
Cuts at: 4178 4537 4696 518
Size: 359 159 2042
Fragments arranged by size:
2042 1140 999 648 359 264 252 191
159 127 39
MspA1I CmG'CkG
Cuts at: 1606 2061 2226 2363 2747 3492 3636 4313 5461
Size: 455 165 137 384 745 144 677 1148
Cuts at: 5461 5706 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 745 677 455 384 245 165
144 137
NaeI GCC'GGC
Cuts at: 2602 2602
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5668
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6220
NgoMIV G'CCGG_C
Cuts at: 2600 2600
Size: 6220
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6220
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2119 2311 3400 3578 5123 1132
Size: 97 890 192 1089 178 1545 2229
Fragments arranged by size:
2229 1545 1089 890 192 178 97
PasI CC'CwG_GG
Cuts at: 1348 2774 1348
Size: 1426 4794
PciI A'CATG_T
Cuts at: 2115 5119 2115
Size: 3004 3216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2484 3378 1108
Size: 1376 894 3950
Fragments arranged by size:
3950 1376 894
PfoI T'CCnGG_A
Cuts at: 2023 2120 2023
Size: 97 6123
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2770 3007 2770
Size: 237 5983
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2229 2229
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3572 830
Size: 2742 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2061 2226 2363 2747 2061
Size: 165 137 384 5534
Fragments arranged by size:
5534 384 165 137
SacI G_AGCT'C
Cuts at: 2433 2433
Size: 6220
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SapI GCTCTTCn'nnn_
Cuts at: 2841 2841
Size: 6220
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 3516 3516
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 3568 4703 5384 5575 826
Size: 219 2523 1135 681 191 1471
Fragments arranged by size:
2523 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6220
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 4324 5225 5487 820
Size: 197 926 867 1514 901 262 1553
Fragments arranged by size:
1553 1514 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 3578 1132
Size: 2446 3774
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 2073 1517
Size: 556 5664
StyI C'CwwG_G
Cuts at: 513 1065 2832 2994 3659 513
Size: 552 1767 162 665 3074
Fragments arranged by size:
3074 1767 665 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2599 2715 4247 4264 4417 4602 5047
Size: 1255 1019 116 1532 17 153 185 445
Cuts at: 5047 325
Size: 1498
Fragments arranged by size:
1532 1498 1255 1019 445 185 153 116
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3138 4466 6178
Size: 274 80 33 51 1443 1161 1328 1712
Cuts at: 6178 96
Size: 138
Fragments arranged by size:
1712 1443 1328 1161 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6180 6212 6180
Size: 32 6188
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6220
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2459 2459
Size: 6220
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3415 4028 276
Size: 53 83 186 936 1881 613 2468
Fragments arranged by size:
2468 1881 936 613 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SfiI SfoI SrfI SwaI