Restriction map for
pFN21AE6476
(Circular) (Six-base) MAPSORT of: pFN21AE6476.seq Check: 447 from: 1 to: 6142
pFN21AE6476 6142 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 2054 2050
Size: 4 6138
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2970 3306 3339 3485 1937
Size: 1033 336 33 146 4594
Fragments arranged by size:
4594 1033 336 146 33
Acc65I G'GTAC_C
Cuts at: 2885 3427 3436 3463 2885
Size: 542 9 27 5564
Fragments arranged by size:
5564 542 27 9
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2245 3261 3342 4205 5589 5857 1632
Size: 613 1016 81 863 1384 268 1917
Fragments arranged by size:
1917 1384 1016 863 613 268 81
AfeI AGC'GCT
Cuts at: 2252 2252
Size: 6142
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 3885 5457 3885
Size: 1572 4570
ApaLI G'TGCA_C
Cuts at: 2201 2843 4199 5355 2201
Size: 642 1356 1156 2988
Fragments arranged by size:
2988 1356 1156 642
ApoI r'AATT_y
Cuts at: 1493 2168 2638 3451 3677 3829 5938 1493
Size: 675 470 813 226 152 2109 1697
Fragments arranged by size:
2109 1697 813 675 470 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 3467 1870
Size: 73 1524 4545
Fragments arranged by size:
4545 1524 73
BamHI G'GATC_C
Cuts at: 2595 3472 3839 2595
Size: 877 367 4898
Fragments arranged by size:
4898 877 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2567 2675 2885 2918 3427
Size: 325 217 627 780 108 210 33 509
Cuts at: 3427 3436 3463 4918 618
Size: 9 27 1455 1842
Fragments arranged by size:
1842 1455 780 627 509 325 217 210
108 33 27 9
BanII G_rGCy'C
Cuts at: 1391 2583 3246 3461 1391
Size: 1192 663 215 4072
Fragments arranged by size:
4072 1192 663 215
BbeI G_GCGC'C
Cuts at: 2571 2571
Size: 6142
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2206 928
Size: 971 307 4864
Fragments arranged by size:
4864 971 307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4333 4367 1903
Size: 34 2396 34 3678
Fragments arranged by size:
3678 2396 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2423 4753 136
Size: 107 122 71 631 605 751 2330 1525
Fragments arranged by size:
2330 1525 751 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2205 2815 2847 2923 4203 5359 1790
Size: 415 610 32 76 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 610 415 76 32
BmgBI CAC'GTC
Cuts at: 1318 2200 1318
Size: 882 5260
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3319 3873 4831
Size: 416 53 83 399 24 1902 554 958
Cuts at: 4831 442
Size: 1753
Fragments arranged by size:
1902 1753 958 554 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3008 4802 1239
Size: 1769 1794 2579
Fragments arranged by size:
2579 1794 1769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2170 4267 5132 5430 2170
Size: 2097 865 298 2882
Fragments arranged by size:
2882 2097 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3200 4805 882
Size: 617 1701 1605 2219
Fragments arranged by size:
2219 1701 1605 617
BsaAI yAC'GTr
Cuts at: 493 1204 2206 3933 493
Size: 711 1002 1727 2702
Fragments arranged by size:
2702 1727 1002 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 2568 3949 4331
Size: 53 83 186 936 582 453 1381 382
Cuts at: 4331 4966 275
Size: 635 1451
Fragments arranged by size:
1451 1381 936 635 582 453 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4575 4973 5247 5394 5805 4575
Size: 398 274 147 411 4912
Fragments arranged by size:
4912 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5917
Fragments arranged by size:
5917 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6142
BseYI C'CCAG_C
Cuts at: 2702 5345 5841 2702
Size: 2643 496 3003
Fragments arranged by size:
3003 2643 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 2984 1163
Size: 573 1065 183 4321
Fragments arranged by size:
4321 1065 573 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2205 2847 4203 4288 5359 2205
Size: 642 1356 85 1071 2988
Fragments arranged by size:
2988 1356 1071 642 85
BsmI GAATG_Cn'
Cuts at: 2860 3039 3653 3746 2860
Size: 179 614 93 5256
Fragments arranged by size:
5256 614 179 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2583 2815 2847 2923 3246 3461
Size: 399 415 378 232 32 76 323 215
Cuts at: 3461 4203 4288 5359 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 378 323 232
215 85 76 32
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2235 2463 4031 5761 2235
Size: 228 1568 1730 2616
Fragments arranged by size:
2616 1730 1568 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 2054 2526 3497 844
Size: 1206 4 472 971 3489
Fragments arranged by size:
3489 1206 971 472 4
BsrBI CCG'CTC
Cuts at: 1394 1823 2041 4029 1394
Size: 429 218 1988 3507
Fragments arranged by size:
3507 1988 429 218
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 3202 1184
Size: 2018 4124
BstXI CCAn_nnnn'nTGG
Cuts at: 2564 3191 2564
Size: 627 5515
BstYI r'GATC_y
Cuts at: 1972 2595 3472 3839 4224 4241 5693 5779 5791
Size: 623 877 367 385 17 1452 86 12
Cuts at: 5791 6137 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 877 623 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2971 2971
Size: 6142
Bsu36I CC'TnA_GG
Cuts at: 3348 3370 3348
Size: 22 6120
BtgI C'CryG_G
Cuts at: 513 1065 2004 2268 2728 2880 3213 3396 3414
Size: 552 939 264 460 152 333 183 18
Cuts at: 3414 513
Size: 3241
Fragments arranged by size:
3241 939 552 460 333 264 183 152
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 3658 4452 4472 3658
Size: 794 20 5328
Fragments arranged by size:
5328 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2877 3210 3589
Size: 54 1425 50 275 18 1047 333 379
Cuts at: 3589 4478 6107 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1047 889 379 333 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2492 2687 4072 1353
Size: 731 408 195 1385 3423
Fragments arranged by size:
3423 1385 731 408 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3076 4729 5103 5249 387
Size: 1064 138 1487 1653 374 146 1280
Fragments arranged by size:
1653 1487 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2245 3008 3261 3342 4205 4802 5589
Size: 393 613 763 253 81 863 597 787
Cuts at: 5589 5857 1239
Size: 268 1524
Fragments arranged by size:
1524 863 787 763 613 597 393 268
253 81
EcoNI CCTnn'n_nnAGG
Cuts at: 2522 2522
Size: 6142
EcoO109I rG'GnC_Cy
Cuts at: 3352 3374 3352
Size: 22 6120
EcoRI G'AATT_C
Cuts at: 2168 2638 3451 3829 2168
Size: 470 813 378 4481
Fragments arranged by size:
4481 813 470 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3134 3134
Size: 6142
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2254 2268 2315 2571 5289 1678
Size: 576 14 47 256 2718 2531
Fragments arranged by size:
2718 2531 576 256 47 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2739 2771 3178 3210 4783
Size: 32 135 32 1167 32 407 32 1573
Cuts at: 4783 4815 4857 4889 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1573 1167 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2188 748
Size: 1440 4702
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KasI G'GCGC_C
Cuts at: 2567 2567
Size: 6142
KpnI G_GTAC'C
Cuts at: 2889 3431 3440 3467 2889
Size: 542 9 27 5564
Fragments arranged by size:
5564 542 27 9
MfeI C'AATT_G
Cuts at: 3741 3741
Size: 6142
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 5256 5440 1305
Size: 1329 2622 184 2007
Fragments arranged by size:
2622 2007 1329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3212 10
Size: 54 1425 343 1380 2940
Fragments arranged by size:
2940 1425 1380 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2360 3236 4100 4459 4618 518
Size: 648 1194 876 864 359 159 2042
Fragments arranged by size:
2042 1194 876 864 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2028 2455 2891 3080 3558 4235 5383 5628
Size: 422 427 436 189 478 677 1148 245
Cuts at: 5628 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 478 436 427 422 245
189
NarI GG'CG_CC
Cuts at: 2568 2568
Size: 6142
NcoI C'CATG_G
Cuts at: 513 1065 2004 2728 3213 3396 3414 513
Size: 552 939 724 485 183 18 3241
Fragments arranged by size:
3241 939 724 552 485 183 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 3018 3500 5045 1132
Size: 97 1789 482 1545 2229
Fragments arranged by size:
2229 1789 1545 482 97
PasI CC'CwG_GG
Cuts at: 1348 2959 1348
Size: 1611 4531
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 5240
PfoI T'CCnGG_A
Cuts at: 2193 2985 3006 2193
Size: 792 21 5329
Fragments arranged by size:
5329 792 21
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PmlI CAC'GTG
Cuts at: 2206 2206
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3352 3374 3352
Size: 22 6120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2156 2188 2156
Size: 32 6110
PstI C_TGCA'G
Cuts at: 830 2061 2250 3494 830
Size: 1231 189 1244 3478
Fragments arranged by size:
3478 1244 1231 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2455 2455
Size: 6142
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 2061 3494 2061
Size: 1433 4709
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 2057 2246 3490 4625 5306 5497 826
Size: 219 1012 189 1244 1135 681 191 1471
Fragments arranged by size:
1471 1244 1135 1012 681 219 191 189
SfoI GGC'GCC
Cuts at: 2569 2569
Size: 6142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2149 4246 5147 5409 820
Size: 197 926 206 2097 901 262 1553
Fragments arranged by size:
2097 1553 926 901 262 206 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3500 1132
Size: 2368 3774
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2004 2728 2772 3213 3396 3414 3432
Size: 552 939 724 44 441 183 18 18
Cuts at: 3432 3581 513
Size: 149 3074
Fragments arranged by size:
3074 939 724 552 441 183 149 44
18 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3056 3215 4169 4186 4339 4524 4969
Size: 1255 1476 159 954 17 153 185 445
Cuts at: 4969 325
Size: 1498
Fragments arranged by size:
1498 1476 1255 954 445 185 159 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 4388 6100
Size: 274 80 33 51 1443 717 1694 1712
Cuts at: 6100 96
Size: 138
Fragments arranged by size:
1712 1694 1443 717 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6102 6134 6102
Size: 32 6110
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3220 3220
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 2168 2501 4271 2168
Size: 333 1770 4039
Fragments arranged by size:
4039 1770 333
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI