Restriction map for
pFN21AE6475
(Circular) (Six-base) MAPSORT of: pFN21AE6475.seq Check: 9717 from: 1 to: 6124
pFN21AE6475 6124 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2037 2267 4187 5571 5839 1632
Size: 405 230 1920 1384 268 1917
Fragments arranged by size:
1920 1917 1384 405 268 230
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2156 2778 5023 6000 1203
Size: 953 622 2245 977 1327
Fragments arranged by size:
2245 1327 977 953 622
AgeI A'CCGG_T
Cuts at: 3221 4955 3221
Size: 1734 4390
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 2276 3335 3380 1166
Size: 1110 1059 45 3910
Fragments arranged by size:
3910 1110 1059 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2294 1184
Size: 1110 5014
AlwNI CAG_nnn'CTG
Cuts at: 2657 2750 3867 5439 2657
Size: 93 1117 1572 3342
Fragments arranged by size:
3342 1572 1117 93
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2228 3433 3659 3811 5920 1493
Size: 735 1205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 735 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3260 4678 160
Size: 872 2228 1418 1606
Fragments arranged by size:
2228 1606 1418 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 3449 1870
Size: 73 1506 4545
Fragments arranged by size:
4545 1506 73
AvrII C'CTAG_G
Cuts at: 2581 2788 2581
Size: 207 5917
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2866 3445 4900 618
Size: 325 217 627 1079 579 1455 1842
Fragments arranged by size:
1842 1455 1079 627 579 325 217
BanII G_rGCy'C
Cuts at: 1391 3129 3443 1391
Size: 1738 314 4072
Fragments arranged by size:
4072 1738 314
BbeI G_GCGC'C
Cuts at: 2870 2870
Size: 6124
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2659 3236 928
Size: 971 760 577 3816
Fragments arranged by size:
3816 971 760 577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4016 5226 4016
Size: 1210 4914
BclI T'GATC_A
Cuts at: 1548 3372 3384 1548
Size: 1824 12 4288
Fragments arranged by size:
4288 1824 12
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4735 136
Size: 107 122 71 631 605 3063 1525
Fragments arranged by size:
3063 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6119 6119
Size: 6124
BlpI GC'TnA_GC
Cuts at: 2299 3541 2299
Size: 1242 4882
Bme1580I G_kGCm'C
Cuts at: 1790 4185 5341 1790
Size: 2395 1156 2573
Fragments arranged by size:
2573 2395 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6124
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3855 4813 442
Size: 416 53 83 399 24 2438 958 1753
Fragments arranged by size:
2438 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2072 2374 2970 4784 1239
Size: 833 302 596 1814 2579
Fragments arranged by size:
2579 1814 833 596 302
Bpu10I CC'TnA_GC
Cuts at: 3085 3085
Size: 6124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2277 2545 4249 5114 5412 2277
Size: 268 1704 865 298 2989
Fragments arranged by size:
2989 1704 865 298 268
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2263 4787 882
Size: 617 764 2524 2219
Fragments arranged by size:
2524 2219 764 617
BsaAI yAC'GTr
Cuts at: 493 1204 3025 3915 493
Size: 711 1821 890 2702
Fragments arranged by size:
2702 1821 890 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2793 2867 3931 4313
Size: 53 83 186 936 1260 74 1064 382
Cuts at: 4313 4948 275
Size: 635 1451
Fragments arranged by size:
1451 1260 1064 936 635 382 186 83
74 53
BsaWI w'CCGG_w
Cuts at: 2080 2995 3221 4557 4955 5229 5376 5787 2080
Size: 915 226 1336 398 274 147 411 2417
Fragments arranged by size:
2417 1336 915 411 398 274 226 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2146 2176 1178
Size: 30 165 30 743 30 5126
Fragments arranged by size:
5126 743 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2499 1186
Size: 1313 4811
BseYI C'CCAG_C
Cuts at: 2918 5327 5823 2918
Size: 2409 496 3219
Fragments arranged by size:
3219 2409 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2895 3574 4335 5363 1540
Size: 275 186 894 679 761 1028 2301
Fragments arranged by size:
2301 1028 894 761 679 275 186
BsiHKAI G_wGCw'C
Cuts at: 3129 4185 4270 5341 3129
Size: 1056 85 1071 3912
Fragments arranged by size:
3912 1071 1056 85
BsmI GAATG_Cn'
Cuts at: 2150 3635 3728 2150
Size: 1485 93 4546
Fragments arranged by size:
4546 1485 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3129 3443 4185 4270 5341 1391
Size: 399 1339 314 742 85 1071 2174
Fragments arranged by size:
2174 1339 1071 742 399 314 85
BspEI T'CCGG_A
Cuts at: 2995 5787 2995
Size: 2792 3332
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3233 3479 844
Size: 2389 246 3489
Fragments arranged by size:
3489 2389 246
BsrBI CCG'CTC
Cuts at: 1394 1823 4011 1394
Size: 429 2188 3507
Fragments arranged by size:
3507 2188 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2316 3033 3129 3305 3387 4619
Size: 1183 879 189 717 96 176 82 1232
Cuts at: 4619 4793 65
Size: 174 1396
Fragments arranged by size:
1396 1232 1183 879 717 189 176 174
96 82
BsrFI r'CCGG_y
Cuts at: 1700 1810 2863 3221 4768 4955 1700
Size: 110 1053 358 1547 187 2869
Fragments arranged by size:
2869 1547 1053 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6124
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6124
BstXI CCAn_nnnn'nTGG
Cuts at: 3073 3073
Size: 6124
BstYI r'GATC_y
Cuts at: 1972 3454 3821 4206 4223 5675 5761 5773 6119
Size: 1482 367 385 17 1452 86 12 346
Cuts at: 6119 1972
Size: 1977
Fragments arranged by size:
1977 1482 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2635 2730 3072 513
Size: 552 939 631 95 342 3565
Fragments arranged by size:
3565 939 631 552 342 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5385
Fragments arranged by size:
5385 683 56
BtsI GCAGTG_nn'
Cuts at: 3375 3640 4434 4454 3375
Size: 265 794 20 5045
Fragments arranged by size:
5045 794 265 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 2851 3428 2851
Size: 577 5547
DraIII CAC_nnn'GTG
Cuts at: 3019 3019
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2607 3075 3571
Size: 54 1425 50 275 18 777 468 496
Cuts at: 3571 4460 6089 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 777 496 468 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3571 1537
Size: 275 1759 4090
Fragments arranged by size:
4090 1759 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2353 3014 3170 4054 1353
Size: 1000 661 156 884 3423
Fragments arranged by size:
3423 1000 884 661 156
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3127 3127
Size: 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2037 2072 2267 2374 2970 4187 4784
Size: 393 405 35 195 107 596 1217 597
Cuts at: 4784 5571 5839 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1217 787 597 596 405 393 268
195 107 35
EcoNI CCTnn'n_nnAGG
Cuts at: 3209 3209
Size: 6124
EcoO109I rG'GnC_Cy
Cuts at: 2408 2408
Size: 6124
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
FspI TGC'GCA
Cuts at: 4630 4630
Size: 6124
HaeII r_GCGC'y
Cuts at: 1678 2870 5271 1678
Size: 1192 2401 2531
Fragments arranged by size:
2531 2401 1192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2520 2552 3376
Size: 32 135 32 671 32 245 32 824
Cuts at: 3376 3408 4765 4797 4839 4871 1373
Size: 32 1357 32 42 32 2626
Fragments arranged by size:
2626 1357 824 671 245 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3112 3468 3714 669
Size: 988 281 1174 356 246 3079
Fragments arranged by size:
3079 1174 988 356 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KasI G'GCGC_C
Cuts at: 2866 2866
Size: 6124
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2938 3031 5238 5422 1305
Size: 1633 93 2207 184 2007
Fragments arranged by size:
2207 2007 1633 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2609 3077 10
Size: 54 1425 343 777 468 3057
Fragments arranged by size:
3057 1425 777 468 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2155 2189 2276 2546 2675 3071 3335
Size: 648 989 34 87 270 129 396 264
Cuts at: 3335 3380 4082 4441 4600 518
Size: 45 702 359 159 2042
Fragments arranged by size:
2042 989 702 648 396 359 270 264
159 129 87 45 34
MspA1I CmG'CkG
Cuts at: 1606 2321 2657 2918 3540 4217 5365 5610 1606
Size: 715 336 261 622 677 1148 245 2120
Fragments arranged by size:
2120 1148 715 677 622 336 261 245
NaeI GCC'GGC
Cuts at: 2865 2865
Size: 6124
NarI GG'CG_CC
Cuts at: 2867 2867
Size: 6124
NcoI C'CATG_G
Cuts at: 513 1065 2004 3072 513
Size: 552 939 1068 3565
Fragments arranged by size:
3565 1068 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NgoMIV G'CCGG_C
Cuts at: 2863 2863
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 3571 3571
Size: 6124
NspI r_CATG'y
Cuts at: 1132 1229 2160 2666 3482 5027 1132
Size: 97 931 506 816 1545 2229
Fragments arranged by size:
2229 1545 931 816 506 97
PasI CC'CwG_GG
Cuts at: 1348 3136 1348
Size: 1788 4336
PciI A'CATG_T
Cuts at: 2156 5023 2156
Size: 2867 3257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2336 1108
Size: 1228 4896
PfoI T'CCnGG_A
Cuts at: 2088 2479 2088
Size: 391 5733
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2953 2985 4193 4225 5706 5738 2953
Size: 32 1208 32 1481 32 3339
Fragments arranged by size:
3339 1481 1208 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2408 2408
Size: 6124
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PstI C_TGCA'G
Cuts at: 830 3476 830
Size: 2646 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
PvuII CAG'CTG
Cuts at: 2321 2657 2918 2321
Size: 336 261 5527
Fragments arranged by size:
5527 336 261
SacI G_AGCT'C
Cuts at: 3129 3129
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 4372 6084 4372
Size: 1712 4412
SexAI A'CCwGG_T
Cuts at: 3105 3105
Size: 6124
SfcI C'TryA_G
Cuts at: 826 1045 2590 3472 4607 5288 5479 826
Size: 219 1545 882 1135 681 191 1471
Fragments arranged by size:
1545 1471 1135 882 681 219 191
SfoI GGC'GCC
Cuts at: 2868 2868
Size: 6124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 3451 1872
Size: 1579 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2292 2524 4228 5129 5391 820
Size: 197 926 349 232 1704 901 262 1553
Fragments arranged by size:
1704 1553 926 901 349 262 232 197
SnaBI TAC'GTA
Cuts at: 493 3025 3915 493
Size: 2532 890 2702
Fragments arranged by size:
2702 2532 890
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 2334 4048 5
Size: 47 2282 1714 2081
Fragments arranged by size:
2282 2081 1714 47
StuI AGG'CCT
Cuts at: 1517 2065 1517
Size: 548 5576
StyI C'CwwG_G
Cuts at: 513 1065 2004 2581 2788 3072 3563 513
Size: 552 939 577 207 284 491 3074
Fragments arranged by size:
3074 939 577 552 491 284 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2237 2813 4151 4168 4321 4506 4951
Size: 1255 657 576 1338 17 153 185 445
Cuts at: 4951 325
Size: 1498
Fragments arranged by size:
1498 1338 1255 657 576 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2159 2343 2741
Size: 274 80 33 51 1443 182 184 398
Cuts at: 2741 4370 6082 96
Size: 1629 1712 138
Fragments arranged by size:
1712 1629 1443 398 274 184 182 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2146 2178 3045 3077 6084 6116 2146
Size: 32 867 32 3007 32 2154
Fragments arranged by size:
3007 2154 867 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2255 1316
Size: 939 5185
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 3449 1870
Size: 1579 4545
XmnI GAAnn'nnTTC
Cuts at: 2725 4253 2725
Size: 1528 4596
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI