Restriction map for
pFN21AE6473
(Circular) (Six-base) MAPSORT of: pFN21AE6473.seq Check: 8366 from: 1 to: 6064
pFN21AE6473 6064 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2346 3407 1937
Size: 409 1061 4594
Fragments arranged by size:
4594 1061 409
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 4191 4564 1563
Size: 2628 373 3063
Fragments arranged by size:
3063 2628 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 3152 4127 5511 5779 1632
Size: 776 744 975 1384 268 1917
Fragments arranged by size:
1917 1384 975 776 744 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 4963 5940 1203
Size: 3760 977 1327
Fragments arranged by size:
3760 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 4793 4793
Size: 6064
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 2296 2355 3097 3260 3807 5379 2296
Size: 59 742 163 547 1572 2981
Fragments arranged by size:
2981 1572 742 547 163 59
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 3373 3599 3751 5860 1493
Size: 1880 226 152 2109 1697
Fragments arranged by size:
2109 1880 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4618 160
Size: 872 3586 1606
Fragments arranged by size:
3586 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2642 2676 3389 1870
Size: 73 126 573 34 713 4545
Fragments arranged by size:
4545 713 573 126 73 34
AvrII C'CTAG_G
Cuts at: 3245 3245
Size: 6064
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 3385 4840 618
Size: 325 217 627 386 1212 1455 1842
Fragments arranged by size:
1842 1455 1212 627 386 325 217
BanII G_rGCy'C
Cuts at: 1391 2553 3066 3383 1391
Size: 1162 513 317 4072
Fragments arranged by size:
4072 1162 513 317
BbeI G_GCGC'C
Cuts at: 2177 2177
Size: 6064
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2371 928
Size: 971 472 4621
Fragments arranged by size:
4621 971 472
BbvCI CC'TCA_GC
Cuts at: 2442 2484 2442
Size: 42 6022
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4255 4289 1903
Size: 34 2318 34 3678
Fragments arranged by size:
3678 2318 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3166 3956 5166 3166
Size: 790 1210 4064
Fragments arranged by size:
4064 1210 790
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6064
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2816 2981 4675
Size: 107 122 71 631 605 1144 165 1694
Cuts at: 4675 136
Size: 1525
Fragments arranged by size:
1694 1525 1144 631 605 165 122 107
71
BglII A'GATC_T
Cuts at: 6059 6059
Size: 6064
BlpI GC'TnA_GC
Cuts at: 2186 2552 3481 2186
Size: 366 929 4769
Fragments arranged by size:
4769 929 366
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6064
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2158 3795 4753
Size: 416 53 83 399 24 741 1637 958
Cuts at: 4753 442
Size: 1753
Fragments arranged by size:
1753 1637 958 741 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2685 3164 4724 1239
Size: 1446 479 1560 2579
Fragments arranged by size:
2579 1560 1446 479
Bpu10I CC'TnA_GC
Cuts at: 2442 2484 2442
Size: 42 6022
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2381 2843 3299 4189 5054 5352 2381
Size: 462 456 890 865 298 3093
Fragments arranged by size:
3093 890 865 462 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 2701 2859 4727 882
Size: 617 541 661 158 1868 2219
Fragments arranged by size:
2219 1868 661 617 541 158
BsaAI yAC'GTr
Cuts at: 493 1204 2687 3855 493
Size: 711 1483 1168 2702
Fragments arranged by size:
2702 1483 1168 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2174 3871 4253 4888
Size: 53 83 186 936 641 1697 382 635
Cuts at: 4888 275
Size: 1451
Fragments arranged by size:
1697 1451 936 641 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2934 4497 4895 5169 5316 5727 2934
Size: 1563 398 274 147 411 3271
Fragments arranged by size:
3271 1563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2081 2111 3294 3324 1178
Size: 30 165 30 678 30 1183 30 3918
Fragments arranged by size:
3918 1183 678 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2102 2137 2459 2501 2512 1186
Size: 907 9 35 322 42 11 4738
Fragments arranged by size:
4738 907 322 42 35 11 9
BseYI C'CCAG_C
Cuts at: 2035 2269 2419 2984 5267 5763 2035
Size: 234 150 565 2283 496 2336
Fragments arranged by size:
2336 2283 565 496 234 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2553 2998 3066 4125 4210 5281 2553
Size: 445 68 1059 85 1071 3336
Fragments arranged by size:
3336 1071 1059 445 85 68
BsmI GAATG_Cn'
Cuts at: 3575 3668 3575
Size: 93 5971
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2553 2998 3066 3383 4125 4210 5281
Size: 399 763 445 68 317 742 85 1071
Cuts at: 5281 1391
Size: 2174
Fragments arranged by size:
2174 1071 763 742 445 399 317 85
68
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 3953 5683 3953
Size: 1730 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2304 3419 844
Size: 1460 1115 3489
Fragments arranged by size:
3489 1460 1115
BsrBI CCG'CTC
Cuts at: 1394 1823 2020 3951 1394
Size: 429 197 1931 3507
Fragments arranged by size:
3507 1931 429 197
BsrDI GCAATG_nn'
Cuts at: 65 1248 4559 4733 65
Size: 1183 3311 174 1396
Fragments arranged by size:
3311 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4708 4895 1700
Size: 110 2898 187 2869
Fragments arranged by size:
2898 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6064
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6064
BssSI C'ACGA_G
Cuts at: 3044 4124 5136 3044
Size: 1080 1012 3972
Fragments arranged by size:
3972 1080 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2296 794
Size: 1502 4562
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6064
BstYI r'GATC_y
Cuts at: 1972 2358 2937 3054 3193 3249 3394 3761 4146
Size: 386 579 117 139 56 145 367 385
Cuts at: 4146 4163 5615 5701 5713 6059 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 579 386 385 367 346 145
139 117 86 56 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2280 2532 513
Size: 552 1215 252 4045
Fragments arranged by size:
4045 1215 552 252
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 3580 4374 4394 3580
Size: 794 20 5250
Fragments arranged by size:
5250 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DraIII CAC_nnn'GTG
Cuts at: 2533 2533
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2337 3511
Size: 54 1425 50 275 18 447 60 1174
Cuts at: 3511 4400 6029 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1174 889 447 275 60 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2621 3994 1353
Size: 1268 1373 3423
Fragments arranged by size:
3423 1373 1268
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4651 5025 5171 387
Size: 1064 138 3062 374 146 1280
Fragments arranged by size:
3062 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2551 3064 2551
Size: 513 5551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2685 3152 3164 4127 4724 5511
Size: 393 776 277 467 12 963 597 787
Cuts at: 5511 5779 1239
Size: 268 1524
Fragments arranged by size:
1524 963 787 776 597 467 393 277
268 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3167 3167
Size: 6064
EcoO109I rG'GnC_Cy
Cuts at: 2032 2293 2449 2589 2032
Size: 261 156 140 5507
Fragments arranged by size:
5507 261 156 140
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2182 3293 2182
Size: 1111 4953
FspI TGC'GCA
Cuts at: 4570 4570
Size: 6064
HaeII r_GCGC'y
Cuts at: 1678 2177 5211 1678
Size: 499 3034 2531
Fragments arranged by size:
3034 2531 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2081 2113 2762 2794 3272
Size: 32 135 32 509 32 649 32 478
Cuts at: 3272 3294 3304 3326 4705 4737 4779 4811 1373
Size: 22 10 22 1379 32 42 32 2626
Fragments arranged by size:
2626 1379 649 509 478 135 42 32
32 32 32 32 32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6064
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KasI G'GCGC_C
Cuts at: 2173 2173
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3338 3663 3338
Size: 325 5739
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 2339 10
Size: 54 1425 343 447 60 3735
Fragments arranged by size:
3735 1425 447 343 60 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4022 4381 4540 518
Size: 648 2856 359 159 2042
Fragments arranged by size:
2856 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2269 2320 2355 2441 2614 2734 2842 3480
Size: 663 51 35 86 173 120 108 638
Cuts at: 3480 4157 5305 5550 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 663 638 245 173 120
108 86 51 35
NarI GG'CG_CC
Cuts at: 2174 2174
Size: 6064
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5512
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NruI TCG'CGA
Cuts at: 2130 2130
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2098 2419 2823 2903 3422 4967 1132
Size: 97 869 321 404 80 519 1545 2229
Fragments arranged by size:
2229 1545 869 519 404 321 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 2533 1108
Size: 1101 324 4639
Fragments arranged by size:
4639 1101 324
PfoI T'CCnGG_A
Cuts at: 2928 2928
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PmlI CAC'GTG
Cuts at: 2687 2687
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2739 2771 4133 4165 5646 5678 2739
Size: 32 1362 32 1481 32 3125
Fragments arranged by size:
3125 1481 1362 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2449 2589 2293
Size: 156 140 5768
Fragments arranged by size:
5768 156 140
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6064
PsiI TTA'TAA
Cuts at: 3634 3634
Size: 6064
PspXI vC'TCGA_Gb
Cuts at: 2642 2642
Size: 6064
PstI C_TGCA'G
Cuts at: 830 2292 2319 2613 2619 3416 830
Size: 1462 27 294 6 797 3478
Fragments arranged by size:
3478 1462 797 294 27 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
PvuII CAG'CTG
Cuts at: 2355 2441 2614 2734 2842 2355
Size: 86 173 120 108 5577
Fragments arranged by size:
5577 173 120 108 86
SacI G_AGCT'C
Cuts at: 2553 3066 2553
Size: 513 5551
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SfcI C'TryA_G
Cuts at: 826 1045 2288 2315 2609 2615 3412 4547 5228
Size: 219 1243 27 294 6 797 1135 681
Cuts at: 5228 5419 826
Size: 191 1471
Fragments arranged by size:
1471 1243 1135 797 681 294 219 191
27 6
SfoI GGC'GCC
Cuts at: 2175 2175
Size: 6064
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2396 2642 2858 3278 4168 5069
Size: 197 926 453 246 216 420 890 901
Cuts at: 5069 5331 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 890 453 420 262 246
216 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 2823 2903 3422 1132
Size: 1691 80 519 3774
Fragments arranged by size:
3774 1691 519 80
SspI AAT'ATT
Cuts at: 5 52 3988 5
Size: 47 3936 2081
Fragments arranged by size:
3936 2081 47
StuI AGG'CCT
Cuts at: 1517 2243 2894 1517
Size: 726 651 4687
Fragments arranged by size:
4687 726 651
StyI C'CwwG_G
Cuts at: 513 1065 2691 3245 3503 513
Size: 552 1626 554 258 3074
Fragments arranged by size:
3074 1626 554 552 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3089 4091 4108 4261 4446 4891 325
Size: 1255 1509 1002 17 153 185 445 1498
Fragments arranged by size:
1509 1498 1255 1002 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4310 6022 96
Size: 274 80 33 51 1443 2333 1712 138
Fragments arranged by size:
2333 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6024 6056 6024
Size: 32 6032
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6064
XbaI T'CTAG_A
Cuts at: 3400 3400
Size: 6064
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3187 3187
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 2642 1943
Size: 699 5365
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 2751 4193 2751
Size: 1442 4622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
NaeI NgoMIV NsiI PacI PspOMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI