Restriction map for
pFN21AE6471
(Circular) (Six-base) MAPSORT of: pFN21AE6471.seq Check: 7412 from: 1 to: 6127
pFN21AE6471 6127 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3095 3095
Size: 6127
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4190 5574 5842 1632
Size: 2558 1384 268 1917
Fragments arranged by size:
2558 1917 1384 268
AfeI AGC'GCT
Cuts at: 2942 2942
Size: 6127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 2297 2396 3393 1166
Size: 1131 99 997 3900
Fragments arranged by size:
3900 1131 997 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2210 3113 1184
Size: 1026 903 4198
Fragments arranged by size:
4198 1026 903
AlwNI CAG_nnn'CTG
Cuts at: 2246 3061 3870 5442 2246
Size: 815 809 1572 2931
Fragments arranged by size:
2931 1572 815 809
ApaI G_GGCC'C
Cuts at: 2555 3220 2555
Size: 665 5462
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 3436 3662 3814 5923 1493
Size: 1943 226 152 2109 1697
Fragments arranged by size:
2109 1943 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 3292 3452 1870
Size: 73 98 1251 160 4545
Fragments arranged by size:
4545 1251 160 98 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3230 3448 4903 618
Size: 325 217 627 330 1113 218 1455 1842
Fragments arranged by size:
1842 1455 1113 627 330 325 218 217
BanII G_rGCy'C
Cuts at: 1391 2131 2467 2555 3220 3446 1391
Size: 740 336 88 665 226 4072
Fragments arranged by size:
4072 740 665 336 226 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2090 2325 2332 2617 2795 928
Size: 971 191 235 7 285 178 4260
Fragments arranged by size:
4260 971 285 235 191 178 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2099 2133 4318 4352 1903
Size: 34 162 34 2185 34 3678
Fragments arranged by size:
3678 2185 162 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BfrBI ATG'CAT
Cuts at: 2349 2349
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3321 3356 4738
Size: 107 122 71 631 605 1649 35 1382
Cuts at: 4738 136
Size: 1525
Fragments arranged by size:
1649 1525 1382 631 605 122 107 71
35
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 2555 3220 4188 5344 1790
Size: 765 665 968 1156 2573
Fragments arranged by size:
2573 1156 968 765 665
BmgBI CAC'GTC
Cuts at: 1318 3183 1318
Size: 1865 4262
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2032 3858 4816
Size: 416 53 83 399 24 615 1826 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1826 1753 958 615 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2329 2954 3182 4787 1239
Size: 1090 625 228 1605 2579
Fragments arranged by size:
2579 1605 1090 625 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 4252 5117 5415 2443
Size: 1809 865 298 3155
Fragments arranged by size:
3155 1809 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2051 2056 2648 4790 882
Size: 617 552 5 592 2142 2219
Fragments arranged by size:
2219 2142 617 592 552 5
BsaAI yAC'GTr
Cuts at: 493 1204 3918 493
Size: 711 2714 2702
Fragments arranged by size:
2714 2702 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2114 2531 4560 4958 5232 5379 5790 2114
Size: 417 2029 398 274 147 411 2451
Fragments arranged by size:
2451 2029 417 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2435 2465 2513 2543 1178
Size: 30 165 30 1032 30 48 30 4762
Fragments arranged by size:
4762 1032 165 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2951 3133 3278 1186
Size: 1065 700 182 145 4035
Fragments arranged by size:
4035 1065 700 182 145
BseYI C'CCAG_C
Cuts at: 2796 2995 3329 5330 5826 2796
Size: 199 334 2001 496 3097
Fragments arranged by size:
3097 2001 496 334 199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2768 3073 3150 1163
Size: 573 1032 305 77 4140
Fragments arranged by size:
4140 1032 573 305 77
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2501 3577 4338 5366 1540
Size: 275 186 500 1076 761 1028 2301
Fragments arranged by size:
2301 1076 1028 761 500 275 186
BsiHKAI G_wGCw'C
Cuts at: 2365 4188 4273 5344 2365
Size: 1823 85 1071 3148
Fragments arranged by size:
3148 1823 1071 85
BsmI GAATG_Cn'
Cuts at: 2349 3070 3638 3731 2349
Size: 721 568 93 4745
Fragments arranged by size:
4745 721 568 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2365 2467 2555 3220 3446 4188
Size: 399 341 234 102 88 665 226 742
Cuts at: 4188 4273 5344 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 665 399 341 234 226
102 88 85
BspEI T'CCGG_A
Cuts at: 5790 5790
Size: 6127
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2420 2850 3095 3482 844
Size: 1576 430 245 387 3489
Fragments arranged by size:
3489 1576 430 387 245
BsrBI CCG'CTC
Cuts at: 1394 1823 2068 4014 1394
Size: 429 245 1946 3507
Fragments arranged by size:
3507 1946 429 245
BsrDI GCAATG_nn'
Cuts at: 65 1248 2702 4622 4796 65
Size: 1183 1454 1920 174 1396
Fragments arranged by size:
1920 1454 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3008 4771 4958 1700
Size: 110 1198 1763 187 2869
Fragments arranged by size:
2869 1763 1198 187 110
BsrGI T'GTAC_A
Cuts at: 96 3039 96
Size: 2943 3184
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3061 3183 794
Size: 2267 122 3738
Fragments arranged by size:
3738 2267 122
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstYI r'GATC_y
Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122
Size: 1485 367 385 17 1452 86 12 346
Cuts at: 6122 1972
Size: 1977
Fragments arranged by size:
1977 1485 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2556 2660 2892 3003 3123 513
Size: 552 939 552 104 232 111 120 3517
Fragments arranged by size:
3517 939 552 552 232 120 111 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2328 3262 487
Size: 683 56 1102 934 3352
Fragments arranged by size:
3352 1102 934 683 56
BtsI GCAGTG_nn'
Cuts at: 3059 3092 3165 3270 3643 4437 4457 3059
Size: 33 73 105 373 794 20 4729
Fragments arranged by size:
4729 794 373 105 73 33 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3154 3189 529
Size: 35 2590 35 3467
Fragments arranged by size:
3467 2590 35 35
DraI TTT'AAA
Cuts at: 2440 3431 2440
Size: 991 5136
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2498 2889 3006
Size: 54 1425 50 275 18 668 391 117
Cuts at: 3006 3357 3574 4463 6092 8
Size: 351 217 889 1629 43
Fragments arranged by size:
1629 1425 889 668 391 351 275 217
117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2498 3574 1537
Size: 275 686 1076 4090
Fragments arranged by size:
4090 1076 686 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4057 1353
Size: 2704 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 4714 5088 5234 387
Size: 1064 138 525 2600 374 146 1280
Fragments arranged by size:
2600 1280 1064 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2329 2954 3182 4190 4787 5574 5842
Size: 393 697 625 228 1008 597 787 268
Cuts at: 5842 1239
Size: 1524
Fragments arranged by size:
1524 1008 787 697 625 597 393 268
228
EcoO109I rG'GnC_Cy
Cuts at: 2552 2695 3216 2552
Size: 143 521 5463
Fragments arranged by size:
5463 521 143
EcoRI G'AATT_C
Cuts at: 3436 3814 3436
Size: 378 5749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2349 2349
Size: 6127
FseI GG_CCGG'CC
Cuts at: 3012 3012
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 2944 5274 1678
Size: 1266 2330 2531
Fragments arranged by size:
2531 2330 1266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2018 2050 4768 4800 4842
Size: 32 135 32 446 32 2718 32 42
Cuts at: 4842 4874 1373
Size: 32 2626
Fragments arranged by size:
2718 2626 446 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2104 2261 5241 5425 1305
Size: 799 157 2980 184 2007
Fragments arranged by size:
2980 2007 799 184 157
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2891 3359 10
Size: 54 1425 343 1059 468 2778
Fragments arranged by size:
2778 1425 1059 468 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2297 2396 3393 4085 4444 4603
Size: 648 1026 105 99 997 692 359 159
Cuts at: 4603 518
Size: 2042
Fragments arranged by size:
2042 1026 997 692 648 359 159 105
99
MspA1I CmG'CkG
Cuts at: 1606 3202 3228 3543 4220 5368 5613 1606
Size: 1596 26 315 677 1148 245 2120
Fragments arranged by size:
2120 1596 1148 677 315 245 26
NaeI GCC'GGC
Cuts at: 3010 3010
Size: 6127
NcoI C'CATG_G
Cuts at: 513 1065 2004 2660 2892 3123 513
Size: 552 939 656 232 231 3517
Fragments arranged by size:
3517 939 656 552 232 231
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NgoMIV G'CCGG_C
Cuts at: 3008 3008
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2351 2351
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2349 2995 3485 5030 1132
Size: 97 1120 646 490 1545 2229
Fragments arranged by size:
2229 1545 1120 646 490 97
PasI CC'CwG_GG
Cuts at: 1348 2802 3019 1348
Size: 1454 217 4456
Fragments arranged by size:
4456 1454 217
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 2551 3216 2551
Size: 665 5462
PstI C_TGCA'G
Cuts at: 830 3265 3479 830
Size: 2435 214 3478
Fragments arranged by size:
3478 2435 214
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 3202 3202
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 2911 3261 3475 4610 5291 5482 826
Size: 219 1866 350 214 1135 681 191 1471
Fragments arranged by size:
1866 1471 1135 681 350 219 214 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2458 4231 5132 5394 820
Size: 197 926 515 1773 901 262 1553
Fragments arranged by size:
1773 1553 926 901 515 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 2349 2995 3485 1132
Size: 1217 646 490 3774
Fragments arranged by size:
3774 1217 646 490
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2004 2224 2660 2892 2958 3111 3123
Size: 552 939 220 436 232 66 153 12
Cuts at: 3123 3566 513
Size: 443 3074
Fragments arranged by size:
3074 939 552 443 436 232 220 153
66 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3116 3326 4154 4171 4324 4509 4954
Size: 1255 1536 210 828 17 153 185 445
Cuts at: 4954 325
Size: 1498
Fragments arranged by size:
1536 1498 1255 828 445 210 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2684 2907 3039
Size: 274 80 33 51 1443 707 223 132
Cuts at: 3039 4373 6085 96
Size: 1334 1712 138
Fragments arranged by size:
1712 1443 1334 707 274 223 138 132
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2435 2467 6087 6119 2435
Size: 32 3620 32 2443
Fragments arranged by size:
3620 2443 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FspAI KasI NarI NruI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI