Restriction map for
pFN21AE6468
(Circular) (Six-base) MAPSORT of: pFN21AE6468.seq Check: 578 from: 1 to: 6481
pFN21AE6468 6481 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2998 3294 2998
Size: 296 6185
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 2337 3802 2337
Size: 1465 5016
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3626 4544 5928 6196 1632
Size: 1994 918 1384 268 1917
Fragments arranged by size:
1994 1917 1384 918 268
AflII C'TTAA_G
Cuts at: 820 1017 3482 820
Size: 197 2465 3819
Fragments arranged by size:
3819 2465 197
AflIII A'CryG_T
Cuts at: 1203 2085 2682 3450 5380 6357 1203
Size: 882 597 768 1930 977 1327
Fragments arranged by size:
1930 1327 977 882 768 597
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6481
AlwNI CAG_nnn'CTG
Cuts at: 4224 5796 4224
Size: 1572 4909
ApaLI G'TGCA_C
Cuts at: 4538 5694 4538
Size: 1156 5325
ApoI r'AATT_y
Cuts at: 1493 3790 4016 4168 6277 1493
Size: 2297 226 152 2109 1697
Fragments arranged by size:
2297 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5035 160
Size: 872 4003 1606
Fragments arranged by size:
4003 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 3806 1870
Size: 73 1863 4545
Fragments arranged by size:
4545 1863 73
BamHI G'GATC_C
Cuts at: 2359 3811 4178 2359
Size: 1452 367 4662
Fragments arranged by size:
4662 1452 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2228 2337 2567 3802 5257
Size: 325 217 627 441 109 230 1235 1455
Cuts at: 5257 618
Size: 1842
Fragments arranged by size:
1842 1455 1235 627 441 325 230 217
109
BanII G_rGCy'C
Cuts at: 1391 3800 1391
Size: 2409 4072
BbeI G_GCGC'C
Cuts at: 2232 2232
Size: 6481
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3562 928
Size: 971 1663 3847
Fragments arranged by size:
3847 1663 971
BbvCI CC'TCA_GC
Cuts at: 3461 3461
Size: 6481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2764 2798 4672 4706 1903
Size: 34 827 34 1874 34 3678
Fragments arranged by size:
3678 1874 827 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6481
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2745 6476 2745
Size: 3731 2750
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 3116 4542 5698 1790
Size: 1326 1426 1156 2573
Fragments arranged by size:
2573 1426 1326 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4212 5170 442
Size: 416 53 83 399 24 2795 958 1753
Fragments arranged by size:
2795 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3319 3319
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 2468 3023 3262 5141 1239
Size: 1015 214 555 239 1879 2579
Fragments arranged by size:
2579 1879 1015 555 239 214
Bpu10I CC'TnA_GC
Cuts at: 3461 3578 3461
Size: 117 6364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3737 4606 5471 5769 3737
Size: 869 865 298 4449
Fragments arranged by size:
4449 869 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2290 5144 882
Size: 617 791 2854 2219
Fragments arranged by size:
2854 2219 791 617
BsaAI yAC'GTr
Cuts at: 493 1204 3451 4272 493
Size: 711 2247 821 2702
Fragments arranged by size:
2702 2247 821 711
BsaBI GATnn'nnATC
Cuts at: 6475 6475
Size: 6481
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2229 2429 2603 3624
Size: 53 83 186 936 696 200 174 1021
Cuts at: 3624 4288 4670 5305 275
Size: 664 382 635 1451
Fragments arranged by size:
1451 1021 936 696 664 635 382 200
186 174 83 53
BsaWI w'CCGG_w
Cuts at: 4914 5312 5586 5733 6144 4914
Size: 398 274 147 411 5251
Fragments arranged by size:
5251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6256
Fragments arranged by size:
6256 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2429 2516 3478 3688 1186
Size: 1243 87 962 210 3979
Fragments arranged by size:
3979 1243 962 210 87
BseYI C'CCAG_C
Cuts at: 3168 3208 3226 3359 5684 6180 3168
Size: 40 18 133 2325 496 3469
Fragments arranged by size:
3469 2325 496 133 40 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2522 1163
Size: 573 786 5122
Fragments arranged by size:
5122 786 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2021 3105 3499 3931 4692 5720
Size: 275 186 20 1084 394 432 761 1028
Cuts at: 5720 1540
Size: 2301
Fragments arranged by size:
2301 1084 1028 761 432 394 275 186
20
BsiHKAI G_wGCw'C
Cuts at: 2625 3186 4542 4627 5698 2625
Size: 561 1356 85 1071 3408
Fragments arranged by size:
3408 1356 1071 561 85
BsiWI C'GTAC_G
Cuts at: 2064 2064
Size: 6481
BsmI GAATG_Cn'
Cuts at: 2175 3125 3992 4085 2175
Size: 950 867 93 4571
Fragments arranged by size:
4571 950 867 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3425 3756 3425
Size: 331 6150
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2625 3116 3186 3800 4542 4627 5698
Size: 399 835 491 70 614 742 85 1071
Cuts at: 5698 1391
Size: 2174
Fragments arranged by size:
2174 1071 835 742 614 491 399 85
70
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2998 3294 3836 844
Size: 2154 296 542 3489
Fragments arranged by size:
3489 2154 542 296
BsrBI CCG'CTC
Cuts at: 1394 1823 2176 4368 1394
Size: 429 353 2192 3507
Fragments arranged by size:
3507 2192 429 353
BsrDI GCAATG_nn'
Cuts at: 65 1248 4976 5150 65
Size: 1183 3728 174 1396
Fragments arranged by size:
3728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2512 3494 5125 5312 1700
Size: 110 702 982 1631 187 2869
Fragments arranged by size:
2869 1631 982 702 187 110
BsrGI T'GTAC_A
Cuts at: 96 2679 96
Size: 2583 3898
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 3641 4541 5553 3641
Size: 900 1012 4569
Fragments arranged by size:
4569 1012 900
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2209 794
Size: 1415 5066
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 2393 1184
Size: 1209 5272
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 3001 3116 3340 2281
Size: 720 115 224 5422
Fragments arranged by size:
5422 720 224 115
BstYI r'GATC_y
Cuts at: 1972 2359 2745 3811 4178 4563 4580 6032 6118
Size: 387 386 1066 367 385 17 1452 86
Cuts at: 6118 6130 6476 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1066 387 386 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2070 2369 2656 3774 513
Size: 552 939 66 299 287 1118 3220
Fragments arranged by size:
3220 1118 939 552 299 287 66
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5742
Fragments arranged by size:
5742 683 56
BtsI GCAGTG_nn'
Cuts at: 2510 2606 3545 3997 4791 4811 2510
Size: 96 939 452 794 20 4180
Fragments arranged by size:
4180 939 794 452 96 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 3785 3785
Size: 6481
DraIII CAC_nnn'GTG
Cuts at: 3708 3708
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3104 5488 809
Size: 507 1788 2384 1802
Fragments arranged by size:
2384 1802 1788 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2018 2709 3102
Size: 54 1425 50 275 18 188 691 393
Cuts at: 3102 3240 3396 3496 3777 3928 4817 6446 8
Size: 138 156 100 281 151 889 1629 43
Fragments arranged by size:
1629 1425 889 691 393 281 275 188
156 151 138 100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2018 3102 3496 3928 1537
Size: 275 206 1084 394 432 4090
Fragments arranged by size:
4090 1084 432 394 275 206
EarI CTCTTCn'nnn_
Cuts at: 1353 2348 4411 1353
Size: 995 2063 3423
Fragments arranged by size:
3423 2063 995
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2136 5068 5442 5588 387
Size: 1064 138 547 2932 374 146 1280
Fragments arranged by size:
2932 1280 1064 547 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2468 3023 3262 3626 4544 5141
Size: 393 622 214 555 239 364 918 597
Cuts at: 5141 5928 6196 1239
Size: 787 268 1524
Fragments arranged by size:
1524 918 787 622 597 555 393 364
268 239 214
EcoNI CCTnn'n_nnAGG
Cuts at: 3005 3290 3005
Size: 285 6196
EcoO109I rG'GnC_Cy
Cuts at: 2644 3600 2644
Size: 956 5525
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2929 2929
Size: 6481
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 2232 2519 5628 1678
Size: 554 287 3109 2531
Fragments arranged by size:
3109 2531 554 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2414 2446 5122 5154 5196
Size: 32 135 32 842 32 2676 32 42
Cuts at: 5196 5228 1373
Size: 32 2626
Fragments arranged by size:
2676 2626 842 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3325 3825 4071 669
Size: 988 281 1387 500 246 3079
Fragments arranged by size:
3079 1387 988 500 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6481
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KasI G'GCGC_C
Cuts at: 2228 2228
Size: 6481
KpnI G_GTAC'C
Cuts at: 2341 3806 2341
Size: 1465 5016
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2835 5595 5779 1305
Size: 1530 2760 184 2007
Fragments arranged by size:
2760 2007 1530 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2711 10
Size: 54 1425 343 879 3780
Fragments arranged by size:
3780 1425 879 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2150 2681 2837 3161 3338 4439 4798
Size: 648 984 531 156 324 177 1101 359
Cuts at: 4798 4957 518
Size: 159 2042
Fragments arranged by size:
2042 1101 984 648 531 359 324 177
159 156
MspA1I CmG'CkG
Cuts at: 1606 2023 2707 3172 3239 3897 4574 5722 5967
Size: 417 684 465 67 658 677 1148 245
Cuts at: 5967 1606
Size: 2120
Fragments arranged by size:
2120 1148 684 677 658 465 417 245
67
NaeI GCC'GGC
Cuts at: 2514 2514
Size: 6481
NarI GG'CG_CC
Cuts at: 2229 2229
Size: 6481
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4990
Fragments arranged by size:
4990 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6481
NgoMIV G'CCGG_C
Cuts at: 2512 2512
Size: 6481
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 2018 3928 2018
Size: 1910 4571
NspI r_CATG'y
Cuts at: 1132 1229 2212 2686 3226 3839 5384 1132
Size: 97 983 474 540 613 1545 2229
Fragments arranged by size:
2229 1545 983 613 540 474 97
PasI CC'CwG_GG
Cuts at: 1348 3631 3727 1348
Size: 2283 96 4102
Fragments arranged by size:
4102 2283 96
PciI A'CATG_T
Cuts at: 2682 5380 2682
Size: 2698 3783
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2390 2724 1108
Size: 1282 334 4865
Fragments arranged by size:
4865 1282 334
PfoI T'CCnGG_A
Cuts at: 2355 2484 2355
Size: 129 6352
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3600 3600
Size: 6481
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PstI C_TGCA'G
Cuts at: 830 3080 3833 830
Size: 2250 753 3478
Fragments arranged by size:
3478 2250 753
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 2707 3172 2707
Size: 465 6016
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SanDI GG'GwC_CC
Cuts at: 3600 3600
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 2444 4729 6441 2444
Size: 2285 1712 2484
Fragments arranged by size:
2484 2285 1712
SfcI C'TryA_G
Cuts at: 826 1045 2039 3076 3829 4964 5645 5836 826
Size: 219 994 1037 753 1135 681 191 1471
Fragments arranged by size:
1471 1135 1037 994 753 681 219 191
SfoI GGC'GCC
Cuts at: 2230 2230
Size: 6481
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3482 3752 4585 5486 5748 820
Size: 197 926 1539 270 833 901 262 1553
Fragments arranged by size:
1553 1539 926 901 833 270 262 197
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 2212 3226 3839 1132
Size: 1080 1014 613 3774
Fragments arranged by size:
3774 1080 1014 613
SspI AAT'ATT
Cuts at: 5 52 4405 5
Size: 47 4353 2081
Fragments arranged by size:
4353 2081 47
StuI AGG'CCT
Cuts at: 1517 2723 1517
Size: 1206 5275
StyI C'CwwG_G
Cuts at: 513 1065 2004 2718 3291 3920 513
Size: 552 939 714 573 629 3074
Fragments arranged by size:
3074 939 714 629 573 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2756 4508 4525 4678 4863 5308 325
Size: 1255 1176 1752 17 153 185 445 1498
Fragments arranged by size:
1752 1498 1255 1176 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2442 2679
Size: 274 80 33 51 1443 327 138 237
Cuts at: 2679 4727 6439 96
Size: 2048 1712 138
Fragments arranged by size:
2048 1712 1443 327 274 237 138 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 3817 3817
Size: 6481
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2584 3154 3307 2584
Size: 570 153 5758
Fragments arranged by size:
5758 570 153
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 4610 4610
Size: 6481
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BstZ17I
Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SrfI SwaI