Restriction map for
pFN21AE6466
(Circular) (Six-base) MAPSORT of: pFN21AE6466.seq Check: 6314 from: 1 to: 6547
pFN21AE6466 6547 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4357 278
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2397 3890 1937
Size: 460 1493 4594
Fragments arranged by size:
4594 1493 460
Acc65I G'GTAC_C
Cuts at: 3868 3868
Size: 6547
AclI AA'CG_TT
Cuts at: 1563 4674 5047 1563
Size: 3111 373 3063
Fragments arranged by size:
3111 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3084 3701 4610 5994 6262 1632
Size: 1452 617 909 1384 268 1917
Fragments arranged by size:
1917 1452 1384 909 617 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6350
AflIII A'CryG_T
Cuts at: 1203 3231 5446 6423 1203
Size: 2028 2215 977 1327
Fragments arranged by size:
2215 2028 1327 977
AgeI A'CCGG_T
Cuts at: 5378 5378
Size: 6547
AhdI GACnn_n'nnGTC
Cuts at: 3690 5276 3690
Size: 1586 4961
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6547
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3401 1184
Size: 2217 4330
AlwNI CAG_nnn'CTG
Cuts at: 3083 4290 5862 3083
Size: 1207 1572 3768
Fragments arranged by size:
3768 1572 1207
ApaI G_GGCC'C
Cuts at: 3798 3798
Size: 6547
ApaLI G'TGCA_C
Cuts at: 4604 5760 4604
Size: 1156 5391
ApoI r'AATT_y
Cuts at: 1493 2551 2573 3856 4082 4234 6343 1493
Size: 1058 22 1283 226 152 2109 1697
Fragments arranged by size:
2109 1697 1283 1058 226 152 22
AseI AT'TA_AT
Cuts at: 160 1032 5101 160
Size: 872 4069 1606
Fragments arranged by size:
4069 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2605 3687 3872 1870
Size: 73 117 545 1082 185 4545
Fragments arranged by size:
4545 1082 545 185 117 73
AvrII C'CTAG_G
Cuts at: 3175 3175
Size: 6547
BamHI G'GATC_C
Cuts at: 3877 4244 3877
Size: 367 6180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2867 2904 3052 3868 5323
Size: 325 217 627 1080 37 148 816 1455
Cuts at: 5323 618
Size: 1842
Fragments arranged by size:
1842 1455 1080 816 627 325 217 148
37
BanII G_rGCy'C
Cuts at: 1391 2425 2627 3273 3798 3866 1391
Size: 1034 202 646 525 68 4072
Fragments arranged by size:
4072 1034 646 525 202 68
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2094 3007 3689 928
Size: 971 195 913 682 3786
Fragments arranged by size:
3786 971 913 682 195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4738 4772 1903
Size: 34 2801 34 3678
Fragments arranged by size:
3678 2801 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3602 4439 5649 3602
Size: 837 1210 4500
Fragments arranged by size:
4500 1210 837
BclI T'GATC_A
Cuts at: 1548 2766 1548
Size: 1218 5329
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5158 136
Size: 107 122 71 631 605 3486 1525
Fragments arranged by size:
3486 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2334 2874 3498 6542 2334
Size: 540 624 3044 2339
Fragments arranged by size:
3044 2339 624 540
BlpI GC'TnA_GC
Cuts at: 3964 3964
Size: 6547
Bme1580I G_kGCm'C
Cuts at: 1790 2870 3057 3798 4608 5764 1790
Size: 1080 187 741 810 1156 2573
Fragments arranged by size:
2573 1156 1080 810 741 187
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6547
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4278 5236 442
Size: 416 53 83 399 24 2861 958 1753
Fragments arranged by size:
2861 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6547
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2203 2300 2347 3718 5207 1239
Size: 964 97 47 1371 1489 2579
Fragments arranged by size:
2579 1489 1371 964 97 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 3557 3574 4672 5537 5835 2822
Size: 735 17 1098 865 298 3534
Fragments arranged by size:
3534 1098 865 735 298 17
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2053 3830 5210 882
Size: 617 554 1777 1380 2219
Fragments arranged by size:
2219 1777 1380 617 554
BsaAI yAC'GTr
Cuts at: 493 1204 4338 493
Size: 711 3134 2702
Fragments arranged by size:
3134 2702 711
BsaBI GATnn'nnATC
Cuts at: 3341 6541 3341
Size: 3200 3347
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4354 4736 5371 275
Size: 53 83 186 936 2821 382 635 1451
Fragments arranged by size:
2821 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3249 4980 5378 5652 5799 6210 3249
Size: 1731 398 274 147 411 3586
Fragments arranged by size:
3586 1731 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2273 2303 2609 2639 1178
Size: 30 165 30 870 30 306 30 5086
Fragments arranged by size:
5086 870 306 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 3263 1186
Size: 892 1185 4470
Fragments arranged by size:
4470 1185 892
BseYI C'CCAG_C
Cuts at: 2170 2634 3002 3135 3423 5750 6246 2170
Size: 464 368 133 288 2327 496 2471
Fragments arranged by size:
2471 2327 496 464 368 288 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5974
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3997 4758 5786 1540
Size: 275 186 1996 761 1028 2301
Fragments arranged by size:
2301 1996 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 4608 4693 5764 3273
Size: 1335 85 1071 4056
Fragments arranged by size:
4056 1335 1071 85
BsmI GAATG_Cn'
Cuts at: 4058 4151 4058
Size: 93 6454
BsmBI CGTCTCn'nnnn_
Cuts at: 2578 2578
Size: 6547
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2425 2627 2870 3057 3273 3798 3866
Size: 399 635 202 243 187 216 525 68
Cuts at: 3866 4608 4693 5764 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 635 525 399 243 216
202 187 85 68
BspEI T'CCGG_A
Cuts at: 3249 6210 3249
Size: 2961 3586
BspHI T'CATG_A
Cuts at: 4436 6166 4436
Size: 1730 4817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2480 3902 844
Size: 1636 1422 3489
Fragments arranged by size:
3489 1636 1422
BsrBI CCG'CTC
Cuts at: 1394 1823 3258 4434 1394
Size: 429 1435 1176 3507
Fragments arranged by size:
3507 1435 1176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2182 2497 5042 5216 65
Size: 1183 934 315 2545 174 1396
Fragments arranged by size:
2545 1396 1183 934 315 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2528 3477 5191 5378 1700
Size: 110 718 949 1714 187 2869
Fragments arranged by size:
2869 1714 949 718 187 110
BsrGI T'GTAC_A
Cuts at: 96 3339 96
Size: 3243 3304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6547
BssSI C'ACGA_G
Cuts at: 4607 5619 4607
Size: 1012 5535
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3052 794
Size: 2258 4289
BstBI TT'CG_AA
Cuts at: 5351 5351
Size: 6547
BstEII G'GTnAC_C
Cuts at: 1184 3322 1184
Size: 2138 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 2357 2998 2131
Size: 226 641 5680
Fragments arranged by size:
5680 641 226
BstYI r'GATC_y
Cuts at: 1972 2334 2874 3498 3877 4244 4629 4646 6098
Size: 362 540 624 379 367 385 17 1452
Cuts at: 6098 6184 6196 6542 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 624 540 385 379 367 362
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2847 2847
Size: 6547
BtgI C'CryG_G
Cuts at: 513 1065 2004 3482 3642 513
Size: 552 939 1478 160 3418
Fragments arranged by size:
3418 1478 939 552 160
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2947 3082 3271 487
Size: 683 56 1721 135 189 3763
Fragments arranged by size:
3763 1721 683 189 135 56
BtsI GCAGTG_nn'
Cuts at: 2028 3781 4063 4857 4877 2028
Size: 1753 282 794 20 3698
Fragments arranged by size:
3698 1753 794 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2977 3012 529
Size: 35 2413 35 4064
Fragments arranged by size:
4064 2413 35 35
DraI TTT'AAA
Cuts at: 3851 3851
Size: 6547
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5554 809
Size: 507 4238 1802
Fragments arranged by size:
4238 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 3069 3479
Size: 54 1425 50 275 18 321 918 410
Cuts at: 3479 3534 3781 3994 4883 6512 8
Size: 55 247 213 889 1629 43
Fragments arranged by size:
1629 1425 918 889 410 321 275 247
213 55 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3994 1537
Size: 275 2182 4090
Fragments arranged by size:
4090 2182 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2351 2432 3248 3515 3551 3665 4477 1353
Size: 998 81 816 267 36 114 812 3423
Fragments arranged by size:
3423 998 816 812 267 114 81 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2822 3452 5134 5508 5654 387
Size: 1064 138 1233 630 1682 374 146 1280
Fragments arranged by size:
1682 1280 1233 1064 630 374 146 138
EcoICRI GAG'CTC
Cuts at: 3271 3271
Size: 6547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2203 2300 2347 3084 3701 3718 4610
Size: 393 571 97 47 737 617 17 892
Cuts at: 4610 5207 5994 6262 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 892 787 737 617 597 571 393
268 97 47 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2348 2348
Size: 6547
EcoO109I rG'GnC_Cy
Cuts at: 2056 2842 3080 3692 3795 2056
Size: 786 238 612 103 4808
Fragments arranged by size:
4808 786 612 238 103
EcoRI G'AATT_C
Cuts at: 2573 3856 4234 2573
Size: 1283 378 4886
Fragments arranged by size:
4886 1283 378
FspI TGC'GCA
Cuts at: 5053 5053
Size: 6547
HaeII r_GCGC'y
Cuts at: 1678 5694 1678
Size: 4016 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2609 2641 3676 3708 5188
Size: 32 135 32 1037 32 1035 32 1480
Cuts at: 5188 5220 5262 5294 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1480 1037 1035 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3891 4137 669
Size: 988 281 1953 246 3079
Fragments arranged by size:
3079 1953 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6547
HpaI GTT'AAC
Cuts at: 4137 4137
Size: 6547
KpnI G_GTAC'C
Cuts at: 3872 3872
Size: 6547
MfeI C'AATT_G
Cuts at: 4146 4146
Size: 6547
MluI A'CGCG_T
Cuts at: 6423 6423
Size: 6547
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 5661 5845 1305
Size: 753 3603 184 2007
Fragments arranged by size:
3603 2007 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3536 10
Size: 54 1425 343 1704 3021
Fragments arranged by size:
3021 1704 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2267 3230 4505 4864 5023 518
Size: 648 1101 963 1275 359 159 2042
Fragments arranged by size:
2042 1275 1101 963 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3139 3352 3963 4640 5788 6033 1606
Size: 1533 213 611 677 1148 245 2120
Fragments arranged by size:
2120 1533 1148 677 611 245 213
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 6547
NcoI C'CATG_G
Cuts at: 513 1065 2004 3482 3642 513
Size: 552 939 1478 160 3418
Fragments arranged by size:
3418 1478 939 552 160
NdeI CA'TA_TG
Cuts at: 387 3380 387
Size: 2993 3554
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 6547
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6547
NotI GC'GGCC_GC
Cuts at: 3994 3994
Size: 6547
NspI r_CATG'y
Cuts at: 1132 1229 3235 3905 5450 1132
Size: 97 2006 670 1545 2229
Fragments arranged by size:
2229 2006 1545 670 97
PasI CC'CwG_GG
Cuts at: 1348 2917 3603 1348
Size: 1569 686 4292
Fragments arranged by size:
4292 1569 686
PciI A'CATG_T
Cuts at: 3231 5446 3231
Size: 2215 4332
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2620 3621 1108
Size: 1512 1001 4034
Fragments arranged by size:
4034 1512 1001
PfoI T'CCnGG_A
Cuts at: 2277 2785 2277
Size: 508 6039
PmeI GTTT'AAAC
Cuts at: 3851 3851
Size: 6547
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 6129 6161 4616
Size: 32 1481 32 5002
Fragments arranged by size:
5002 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2056 2842 3080 3692 2056
Size: 786 238 612 4911
Fragments arranged by size:
4911 786 612 238
PshAI GACnn'nnGTC
Cuts at: 1613 3735 1613
Size: 2122 4425
PsiI TTA'TAA
Cuts at: 4117 4117
Size: 6547
PspOMI G'GGCC_C
Cuts at: 3794 3794
Size: 6547
PstI C_TGCA'G
Cuts at: 830 3899 830
Size: 3069 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6547
PvuII CAG'CTG
Cuts at: 3139 3352 3139
Size: 213 6334
RsrII CG'GwC_CG
Cuts at: 3439 3439
Size: 6547
SacI G_AGCT'C
Cuts at: 3273 3273
Size: 6547
SalI G'TCGA_C
Cuts at: 1936 3889 1936
Size: 1953 4594
SanDI GG'GwC_CC
Cuts at: 2056 2056
Size: 6547
SapI GCTCTTCn'nnn_
Cuts at: 3248 3515 3248
Size: 267 6280
SbfI CC_TGCA'GG
Cuts at: 3899 3899
Size: 6547
ScaI AGT'ACT
Cuts at: 4795 6507 4795
Size: 1712 4835
SexAI A'CCwGG_T
Cuts at: 2097 2097
Size: 6547
SfcI C'TryA_G
Cuts at: 826 1045 3895 5030 5711 5902 826
Size: 219 2850 1135 681 191 1471
Fragments arranged by size:
2850 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6547
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6547
SmaI CCC'GGG
Cuts at: 1872 3874 1872
Size: 2002 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 3553 3572 4651 5552 5814
Size: 197 926 894 716 19 1079 901 262
Cuts at: 5814 820
Size: 1553
Fragments arranged by size:
1553 1079 926 901 894 716 262 197
19
SnaBI TAC'GTA
Cuts at: 493 4338 493
Size: 3845 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6547
SphI G_CATG'C
Cuts at: 1132 3905 1132
Size: 2773 3774
SspI AAT'ATT
Cuts at: 5 52 4471 5
Size: 47 4419 2081
Fragments arranged by size:
4419 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6547
StyI C'CwwG_G
Cuts at: 513 1065 2004 2308 3108 3175 3482 3642 3986
Size: 552 939 304 800 67 307 160 344
Cuts at: 3986 513
Size: 3074
Fragments arranged by size:
3074 939 800 552 344 307 304 160
67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2885 3415 4574 4591 4744 4929 5374
Size: 1255 1305 530 1159 17 153 185 445
Cuts at: 5374 325
Size: 1498
Fragments arranged by size:
1498 1305 1255 1159 530 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3339 3561 3576
Size: 274 80 33 51 1443 1362 222 15
Cuts at: 3576 4793 6505 96
Size: 1217 1712 138
Fragments arranged by size:
1712 1443 1362 1217 274 222 138 80
51 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6507 6539 6507
Size: 32 6515
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6547
XbaI T'CTAG_A
Cuts at: 3883 3883
Size: 6547
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2713 2995 3064 2713
Size: 282 69 6196
Fragments arranged by size:
6196 282 69
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6547
XmaI C'CCGG_G
Cuts at: 1870 3872 1870
Size: 2002 4545
XmnI GAAnn'nnTTC
Cuts at: 4676 4676
Size: 6547
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4355 276
Size: 53 83 186 936 2821 2468
Fragments arranged by size:
2821 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspXI
PsrI SacII SfiI SfoI SrfI SwaI