Restriction map for
pFN21AE6462
(Circular) (Six-base) MAPSORT of: pFN21AE6462.seq Check: 5572 from: 1 to: 6187
pFN21AE6462 6187 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3530 1937
Size: 1593 4594
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2074 2591 4250 5634 5902 1632
Size: 442 517 1659 1384 268 1917
Fragments arranged by size:
1917 1659 1384 517 442 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 2178 5086 6063 1203
Size: 975 2908 977 1327
Fragments arranged by size:
2908 1327 977 975
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 2995 4916 2995
Size: 1921 4266
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AlwNI CAG_nnn'CTG
Cuts at: 3930 5502 3930
Size: 1572 4615
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2332 3496 3722 3874 5983 1493
Size: 839 1164 226 152 2109 1697
Fragments arranged by size:
2109 1697 1164 839 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2762 2766 3428 4741 160
Size: 872 1730 4 662 1313 1606
Fragments arranged by size:
1730 1606 1313 872 662 4
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2713 3512 1870
Size: 73 770 799 4545
Fragments arranged by size:
4545 799 770 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3508 4963 618
Size: 325 217 627 1721 1455 1842
Fragments arranged by size:
1842 1721 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3249 3506 1391
Size: 1858 257 4072
Fragments arranged by size:
4072 1858 257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2751 2790 928
Size: 971 852 39 4325
Fragments arranged by size:
4325 971 852 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4378 4412 1903
Size: 34 2441 34 3678
Fragments arranged by size:
3678 2441 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2260 4079 5289 2260
Size: 1819 1210 3158
Fragments arranged by size:
3158 1819 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4798 136
Size: 107 122 71 631 605 3126 1525
Fragments arranged by size:
3126 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2202 6182 2202
Size: 3980 2207
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 4248 5404 1790
Size: 2458 1156 2573
Fragments arranged by size:
2573 2458 1156
BmgBI CAC'GTC
Cuts at: 1318 2181 1318
Size: 863 5324
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2041 2223 3918
Size: 416 53 83 399 24 624 182 1695
Cuts at: 3918 4876 442
Size: 958 1753
Fragments arranged by size:
1753 1695 958 624 416 399 182 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3486 3486
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2227 2569 2895 4847 1239
Size: 988 342 326 1952 2579
Fragments arranged by size:
2579 1952 988 342 326
Bpu10I CC'TnA_GC
Cuts at: 3458 3458
Size: 6187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2040 2549 2714 4850 882
Size: 617 541 509 165 2136 2219
Fragments arranged by size:
2219 2136 617 541 509 165
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 2669 6181 2669
Size: 3512 2675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2642 3994 4376 5011
Size: 53 83 186 936 1109 1352 382 635
Cuts at: 5011 275
Size: 1451
Fragments arranged by size:
1451 1352 1109 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2542 2572 3306 3336 1178
Size: 30 165 30 1139 30 734 30 4029
Fragments arranged by size:
4029 1139 734 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2544 1186
Size: 1358 4829
BseYI C'CCAG_C
Cuts at: 5390 5886 5390
Size: 496 5691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2253 2669 1163
Size: 573 517 416 4681
Fragments arranged by size:
4681 573 517 416
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3637 4398 5426 1540
Size: 275 186 1636 761 1028 2301
Fragments arranged by size:
2301 1636 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2366 3249 4248 4333 5404 2366
Size: 883 999 85 1071 3149
Fragments arranged by size:
3149 1071 999 883 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2366 3249 3506 4248 4333 5404 1391
Size: 399 576 883 257 742 85 1071 2174
Fragments arranged by size:
2174 1071 883 742 576 399 257 85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 3393 4076 5806 3393
Size: 683 1730 3774
Fragments arranged by size:
3774 1730 683
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 844
Size: 2698 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2137 4682 4856 65
Size: 1183 889 2545 174 1396
Fragments arranged by size:
2545 1396 1183 889 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3076 4831 5018 1700
Size: 110 1266 1755 187 2869
Fragments arranged by size:
2869 1755 1266 187 110
BsrGI T'GTAC_A
Cuts at: 96 3404 96
Size: 3308 2879
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 3452 4247 5259 3452
Size: 795 1012 4380
Fragments arranged by size:
4380 1012 795
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6187
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 2202 3517 3884 4269 4286 5738 5824 5836
Size: 230 1315 367 385 17 1452 86 12
Cuts at: 5836 6182 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1315 385 367 346 230 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4696
Fragments arranged by size:
4696 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2353 487
Size: 683 56 1127 4321
Fragments arranged by size:
4321 1127 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 3268 3327 3703 4497 4517 2162
Size: 1106 59 376 794 20 3832
Fragments arranged by size:
3832 1106 794 376 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2012 3273 3634
Size: 54 1425 50 275 18 182 1261 361
Cuts at: 3634 4523 6152 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1261 889 361 275 182 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2371 3062 4117 1353
Size: 932 86 691 1055 3423
Fragments arranged by size:
3423 1055 932 691 86
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2996 4774 5148 5294 387
Size: 1064 138 1407 1778 374 146 1280
Fragments arranged by size:
1778 1407 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3247 3247
Size: 6187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2074 2227 2569 2591 2895 4250 4847
Size: 393 442 153 342 22 304 1355 597
Cuts at: 4847 5634 5902 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1355 787 597 442 393 342 304
268 153 22
EcoO109I rG'GnC_Cy
Cuts at: 3111 3111
Size: 6187
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2264 2264
Size: 6187
FspI TGC'GCA
Cuts at: 4693 4693
Size: 6187
HaeII r_GCGC'y
Cuts at: 1678 3077 5334 1678
Size: 1399 2257 2531
Fragments arranged by size:
2531 2257 1399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2981 3013 4828 4860 4902
Size: 32 135 32 1409 32 1815 32 42
Cuts at: 4902 4934 1373
Size: 32 2626
Fragments arranged by size:
2626 1815 1409 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2848 3267 748
Size: 2100 419 3668
Fragments arranged by size:
3668 2100 419
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3408 3786 3408
Size: 378 5809
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2995 5301 5485 1305
Size: 1690 2306 184 2007
Fragments arranged by size:
2306 2007 1690 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2014 3275 10
Size: 54 1425 343 182 1261 2922
Fragments arranged by size:
2922 1425 1261 343 182 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 3392 4145 4504 4663 518
Size: 648 895 1331 753 359 159 2042
Fragments arranged by size:
2042 1331 895 753 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3603 4280 5428 5673 1606
Size: 1997 677 1148 245 2120
Fragments arranged by size:
2120 1997 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4696
Fragments arranged by size:
4696 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2362 2865 3545 5090 1132
Size: 97 1133 503 680 1545 2229
Fragments arranged by size:
2229 1545 1133 680 503 97
PacI TTA_AT'TAA
Cuts at: 2766 2766
Size: 6187
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2478 1108
Size: 902 468 4817
Fragments arranged by size:
4817 902 468
PfoI T'CCnGG_A
Cuts at: 2872 3348 2872
Size: 476 5711
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3063 3095 4256 4288 5769 5801 3063
Size: 32 1161 32 1481 32 3449
Fragments arranged by size:
3449 1481 1161 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3111 3111
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 1613 3234 1613
Size: 1621 4566
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2469 3322 3539 830
Size: 1639 853 217 3478
Fragments arranged by size:
3478 1639 853 217
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
SacI G_AGCT'C
Cuts at: 3249 3249
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SanDI GG'GwC_CC
Cuts at: 3111 3111
Size: 6187
SapI GCTCTTCn'nnn_
Cuts at: 2371 2371
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2465 3062 3318 3535 4670 5351 5542
Size: 219 1420 597 256 217 1135 681 191
Cuts at: 5542 826
Size: 1471
Fragments arranged by size:
1471 1420 1135 681 597 256 219 217
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4291 5192 5454 820
Size: 197 926 2348 901 262 1553
Fragments arranged by size:
2348 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 3444 4111 5
Size: 47 3392 667 2081
Fragments arranged by size:
3392 2081 667 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 2004 3626 513
Size: 552 939 1622 3074
Fragments arranged by size:
3074 1622 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4214 4231 4384 4569 5014 325
Size: 1255 2634 17 153 185 445 1498
Fragments arranged by size:
2634 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2433 3058 3404
Size: 274 80 33 51 1443 456 625 346
Cuts at: 3404 4433 6145 96
Size: 1029 1712 138
Fragments arranged by size:
1712 1443 1029 625 456 346 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2152 2184 2542 2574 2673 2705 3013 3045 3306
Size: 32 358 32 99 32 308 32 261
Cuts at: 3306 3338 6147 6179 2152
Size: 32 2809 32 2160
Fragments arranged by size:
2809 2160 358 308 261 99 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2641 1316
Size: 1325 4862
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 4316 4316
Size: 6187
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PacI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SexAI SfiI SfoI SrfI SwaI XcmI