Restriction map for
pFN21AE6461
(Circular) (Six-base) MAPSORT of: pFN21AE6461.seq Check: 6125 from: 1 to: 6130
pFN21AE6461 6130 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2030 2049 2950 2030
Size: 19 901 5210
Fragments arranged by size:
5210 901 19
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 3451 3451
Size: 6130
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4193 5577 5845 1632
Size: 2561 1384 268 1917
Fragments arranged by size:
2561 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 3120 3309 5029 6006 1203
Size: 1917 189 1720 977 1327
Fragments arranged by size:
1917 1720 1327 977 189
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 4859 4859
Size: 6130
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2357 1184
Size: 1173 4957
AlwNI CAG_nnn'CTG
Cuts at: 2620 3873 5445 2620
Size: 1253 1572 3305
Fragments arranged by size:
3305 1572 1253
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2012 2056 2190 2366 3135 3455 1870
Size: 73 69 44 134 176 769 320 4545
Fragments arranged by size:
4545 769 320 176 134 73 69 44
BamHI G'GATC_C
Cuts at: 2773 3460 3827 2773
Size: 687 367 5076
Fragments arranged by size:
5076 687 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2808 3451 4906 618
Size: 325 217 627 1021 643 1455 1842
Fragments arranged by size:
1842 1455 1021 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2076 2200 2495 2724 3091 3209 3323
Size: 621 64 124 295 229 367 118 114
Cuts at: 3323 3449 1391
Size: 126 4072
Fragments arranged by size:
4072 621 367 295 229 126 124 118
114 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3016 928
Size: 971 1117 4042
Fragments arranged by size:
4042 1117 971
BbvCI CC'TCA_GC
Cuts at: 2930 2930
Size: 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2728 4022 5232 2728
Size: 1294 1210 3626
Fragments arranged by size:
3626 1294 1210
BclI T'GATC_A
Cuts at: 1548 2779 3054 1548
Size: 1231 275 4624
Fragments arranged by size:
4624 1231 275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2322 3288 6125 2322
Size: 966 2837 2327
Fragments arranged by size:
2837 2327 966
BlpI GC'TnA_GC
Cuts at: 2300 3547 2300
Size: 1247 4883
Bme1580I G_kGCm'C
Cuts at: 1790 2607 2811 2903 4191 5347 1790
Size: 817 204 92 1288 1156 2573
Fragments arranged by size:
2573 1288 1156 817 204 92
BmgBI CAC'GTC
Cuts at: 1318 2911 1318
Size: 1593 4537
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3861 4819 442
Size: 416 53 83 399 24 2444 958 1753
Fragments arranged by size:
2444 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2955 3110 2955
Size: 155 5975
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4790 1239
Size: 3551 2579
Bpu10I CC'TnA_GC
Cuts at: 2930 2930
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2363 4255 5120 5418 2363
Size: 1892 865 298 3075
Fragments arranged by size:
3075 1892 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3076 4793 882
Size: 617 1577 1717 2219
Fragments arranged by size:
2219 1717 1577 617
BsaAI yAC'GTr
Cuts at: 493 1204 3921 493
Size: 711 2717 2702
Fragments arranged by size:
2717 2702 711
BsaBI GATnn'nnATC
Cuts at: 2327 2778 6124 2327
Size: 451 3346 2333
Fragments arranged by size:
3346 2333 451
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 3937 4319 4954
Size: 53 83 186 936 608 1796 382 635
Cuts at: 4954 275
Size: 1451
Fragments arranged by size:
1796 1451 936 635 608 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2153 4563 4961 5235 5382 5793 2153
Size: 2410 398 274 147 411 2490
Fragments arranged by size:
2490 2410 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2396 2426 1178
Size: 30 165 30 993 30 4882
Fragments arranged by size:
4882 993 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2152 2485 3083 3199 3328 1186
Size: 931 35 333 598 116 129 3988
Fragments arranged by size:
3988 931 598 333 129 116 35
BseYI C'CCAG_C
Cuts at: 2290 2344 2479 2488 2542 2600 2711 5333 5829
Size: 54 135 9 54 58 111 2622 496
Cuts at: 5829 2290
Size: 2591
Fragments arranged by size:
2622 2591 496 135 111 58 54 54
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2978 1163
Size: 573 1242 4315
Fragments arranged by size:
4315 1242 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2308 3580 4341 5369 1540
Size: 275 186 307 1272 761 1028 2301
Fragments arranged by size:
2301 1272 1028 761 307 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 4191 4276 5347 2724
Size: 1467 85 1071 3507
Fragments arranged by size:
3507 1467 1071 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2076 2200 2495 2607 2724 2811
Size: 399 222 64 124 295 112 117 87
Cuts at: 2811 2903 3091 3209 3323 3449 4191 4276 5347
Size: 92 188 118 114 126 742 85 1071
Cuts at: 5347 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 295 222 188 126
124 118 117 114 112 92 87 85
64
BspEI T'CCGG_A
Cuts at: 5793 5793
Size: 6130
BspHI T'CATG_A
Cuts at: 3057 4019 5749 3057
Size: 962 1730 3438
Fragments arranged by size:
3438 1730 962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2030 2049 2950 3485 844
Size: 1186 19 901 535 3489
Fragments arranged by size:
3489 1186 901 535 19
BsrBI CCG'CTC
Cuts at: 1394 1823 2511 4017 1394
Size: 429 688 1506 3507
Fragments arranged by size:
3507 1506 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2850 4625 4799 65
Size: 1183 1602 1775 174 1396
Fragments arranged by size:
1775 1602 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3257 4774 4961 1700
Size: 110 1447 1517 187 2869
Fragments arranged by size:
2869 1517 1447 187 110
BsrGI T'GTAC_A
Cuts at: 96 3279 96
Size: 3183 2947
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 2129 4190 5202 2129
Size: 2061 1012 3057
Fragments arranged by size:
3057 2061 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6130
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 2017 1184
Size: 833 5297
BstXI CCAn_nnnn'nTGG
Cuts at: 3010 3010
Size: 6130
BstYI r'GATC_y
Cuts at: 1972 2322 2773 3288 3460 3827 4212 4229 5681
Size: 350 451 515 172 367 385 17 1452
Cuts at: 5681 5767 5779 6125 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 515 451 385 367 350 346
172 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2027 2730 3303 513
Size: 552 962 703 573 3340
Fragments arranged by size:
3340 962 703 573 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2169 487
Size: 683 56 943 4448
Fragments arranged by size:
4448 943 683 56
BtsI GCAGTG_nn'
Cuts at: 2808 3646 4440 4460 2808
Size: 838 794 20 4478
Fragments arranged by size:
4478 838 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5137 809
Size: 507 3821 1802
Fragments arranged by size:
3821 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2396 2703 3357
Size: 54 1425 50 275 18 566 307 654
Cuts at: 3357 3577 4466 6095 8
Size: 220 889 1629 43
Fragments arranged by size:
1629 1425 889 654 566 307 275 220
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2591 4060 1353
Size: 1238 1469 3423
Fragments arranged by size:
3423 1469 1238
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2269 2443 4717 5091 5237 387
Size: 1064 138 680 174 2274 374 146 1280
Fragments arranged by size:
2274 1280 1064 680 374 174 146 138
EcoICRI GAG'CTC
Cuts at: 2722 2722
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4193 4790 5577 5845 1239
Size: 393 2561 597 787 268 1524
Fragments arranged by size:
2561 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2061 2617 2689 2061
Size: 556 72 5502
Fragments arranged by size:
5502 556 72
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2429 2480 5277 1678
Size: 751 51 2797 2531
Fragments arranged by size:
2797 2531 751 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2726 2758 4771 4803 4845
Size: 32 135 32 1154 32 2013 32 42
Cuts at: 4845 4877 1373
Size: 32 2626
Fragments arranged by size:
2626 2013 1154 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6130
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 3455 3455
Size: 6130
MfeI C'AATT_G
Cuts at: 3729 3729
Size: 6130
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2190 2766 2927 5244 5428 1305
Size: 885 576 161 2317 184 2007
Fragments arranged by size:
2317 2007 885 576 184 161
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2398 2705 3359 10
Size: 54 1425 343 566 307 654 2781
Fragments arranged by size:
2781 1425 654 566 343 307 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 3050 3056 3308 3314 4088 4447
Size: 648 1167 717 6 252 6 774 359
Cuts at: 4447 4606 518
Size: 159 2042
Fragments arranged by size:
2042 1167 774 717 648 359 252 159
6 6
MspA1I CmG'CkG
Cuts at: 1606 2029 3033 3241 3546 4223 5371 5616 1606
Size: 423 1004 208 305 677 1148 245 2120
Fragments arranged by size:
2120 1148 1004 677 423 305 245 208
NcoI C'CATG_G
Cuts at: 513 1065 3303 513
Size: 552 2238 3340
Fragments arranged by size:
3340 2238 552
NdeI CA'TA_TG
Cuts at: 387 3409 387
Size: 3022 3108
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2338 3124 3313 3488 5033 1132
Size: 97 1109 786 189 175 1545 2229
Fragments arranged by size:
2229 1545 1109 786 189 175 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 3120 3309 5029 3120
Size: 189 1720 4221
Fragments arranged by size:
4221 1720 189
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2822 1108
Size: 1714 4416
PfoI T'CCnGG_A
Cuts at: 2821 2821
Size: 6130
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2061 2689 2061
Size: 628 5502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PspXI vC'TCGA_Gb
Cuts at: 3135 3135
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2639 2983 3240 3482 830
Size: 1809 344 257 242 3478
Fragments arranged by size:
3478 1809 344 257 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
PvuII CAG'CTG
Cuts at: 3241 3241
Size: 6130
SacI G_AGCT'C
Cuts at: 2724 2724
Size: 6130
SacII CC_GC'GG
Cuts at: 2030 2030
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SbfI CC_TGCA'GG
Cuts at: 3482 3482
Size: 6130
ScaI AGT'ACT
Cuts at: 4378 6090 4378
Size: 1712 4418
SfcI C'TryA_G
Cuts at: 826 1045 2635 2668 2979 3236 3478 4613 5294
Size: 219 1590 33 311 257 242 1135 681
Cuts at: 5294 5485 826
Size: 191 1471
Fragments arranged by size:
1590 1471 1135 681 311 257 242 219
191 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 2192 3457 1872
Size: 320 1265 4545
Fragments arranged by size:
4545 1265 320
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2378 3135 4234 5135 5397 820
Size: 197 926 435 757 1099 901 262 1553
Fragments arranged by size:
1553 1099 926 901 757 435 262 197
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 3246 3303 3569 513
Size: 552 2181 57 266 3074
Fragments arranged by size:
3074 2181 552 266 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2858 3002 4157 4174 4327 4512 4957
Size: 1255 1278 144 1155 17 153 185 445
Cuts at: 4957 325
Size: 1498
Fragments arranged by size:
1498 1278 1255 1155 445 185 153 144
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3279 4376 6088
Size: 274 80 33 51 1443 1302 1097 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
1712 1443 1302 1097 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2758 2790 6090 6122 2758
Size: 32 3300 32 2766
Fragments arranged by size:
3300 2766 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6130
XbaI T'CTAG_A
Cuts at: 2200 3466 2200
Size: 1266 4864
XhoI C'TCGA_G
Cuts at: 1943 3135 1943
Size: 1192 4938
XmaI C'CCGG_G
Cuts at: 1870 2190 3455 1870
Size: 320 1265 4545
Fragments arranged by size:
4545 1265 320
XmnI GAAnn'nnTTC
Cuts at: 4259 4259
Size: 6130
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI