Restriction map for
pFN21AE6460
(Circular) (Six-base) MAPSORT of: pFN21AE6460.seq Check: 1670 from: 1 to: 6112
pFN21AE6460 6112 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2031 2267 2031
Size: 236 5876
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3922 278
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3455 1937
Size: 1518 4594
Acc65I G'GTAC_C
Cuts at: 2118 3433 2118
Size: 1315 4797
AclI AA'CG_TT
Cuts at: 1563 4239 4612 1563
Size: 2676 373 3063
Fragments arranged by size:
3063 2676 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 4175 5559 5827 1632
Size: 723 1820 1384 268 1917
Fragments arranged by size:
1917 1820 1384 723 268
AfeI AGC'GCT
Cuts at: 3336 3336
Size: 6112
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5915
AflIII A'CryG_T
Cuts at: 1203 5011 5988 1203
Size: 3808 977 1327
Fragments arranged by size:
3808 1327 977
AgeI A'CCGG_T
Cuts at: 4943 4943
Size: 6112
AhdI GACnn_n'nnGTC
Cuts at: 4841 4841
Size: 6112
AleI CACnn'nnGTG
Cuts at: 1166 3183 1166
Size: 2017 4095
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2902 1184
Size: 1718 4394
AlwNI CAG_nnn'CTG
Cuts at: 2745 3228 3855 5427 2745
Size: 483 627 1572 3430
Fragments arranged by size:
3430 1572 627 483
ApaI G_GGCC'C
Cuts at: 2169 2828 2169
Size: 659 5453
ApaLI G'TGCA_C
Cuts at: 4169 5325 4169
Size: 1156 4956
ApoI r'AATT_y
Cuts at: 1493 3421 3647 3799 5908 1493
Size: 1928 226 152 2109 1697
Fragments arranged by size:
2109 1928 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4666 160
Size: 872 3634 1606
Fragments arranged by size:
3634 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
AvaI C'yCGr_G
Cuts at: 1870 1943 2108 3437 1870
Size: 73 165 1329 4545
Fragments arranged by size:
4545 1329 165 73
BamHI G'GATC_C
Cuts at: 3442 3809 3442
Size: 367 5745
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2118 3433 4888 618
Size: 325 217 627 331 1315 1455 1842
Fragments arranged by size:
1842 1455 1315 627 331 325 217
BanII G_rGCy'C
Cuts at: 1391 2051 2073 2169 2486 2828 3158 3323 3431
Size: 660 22 96 317 342 330 165 108
Cuts at: 3431 1391
Size: 4072
Fragments arranged by size:
4072 660 342 330 317 165 108 96
22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 2662 2769 928
Size: 971 130 633 107 4271
Fragments arranged by size:
4271 971 633 130 107
BbvCI CC'TCA_GC
Cuts at: 3023 3233 3275 3023
Size: 210 42 5860
Fragments arranged by size:
5860 210 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4303 4337 1903
Size: 34 2366 34 3678
Fragments arranged by size:
3678 2366 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4004 5214 4004
Size: 1210 4902
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2468 2832 4723
Size: 107 122 71 631 605 796 364 1891
Cuts at: 4723 136
Size: 1525
Fragments arranged by size:
1891 1525 796 631 605 364 122 107
71
BglII A'GATC_T
Cuts at: 6107 6107
Size: 6112
BlpI GC'TnA_GC
Cuts at: 3529 3529
Size: 6112
Bme1580I G_kGCm'C
Cuts at: 1790 2169 2223 2828 4173 5329 1790
Size: 379 54 605 1345 1156 2573
Fragments arranged by size:
2573 1345 1156 605 379 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6112
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3190 3843 4801
Size: 416 53 83 399 24 1773 653 958
Cuts at: 4801 442
Size: 1753
Fragments arranged by size:
1773 1753 958 653 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6112
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3356 3356
Size: 6112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2651 2711 4772 1239
Size: 1412 60 2061 2579
Fragments arranged by size:
2579 2061 1412 60
Bpu10I CC'TnA_GC
Cuts at: 3023 3233 3275 3023
Size: 210 42 5860
Fragments arranged by size:
5860 210 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4237 5102 5400 4237
Size: 865 298 4949
Fragments arranged by size:
4949 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2495 3061 3084 4775 882
Size: 617 996 566 23 1691 2219
Fragments arranged by size:
2219 1691 996 617 566 23
BsaAI yAC'GTr
Cuts at: 493 1204 3903 493
Size: 711 2699 2702
Fragments arranged by size:
2702 2699 711
BsaBI GATnn'nnATC
Cuts at: 6106 6106
Size: 6112
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3919 4301 4936 275
Size: 53 83 186 936 2386 382 635 1451
Fragments arranged by size:
2386 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2486 4545 4943 5217 5364 5775 2486
Size: 2059 398 274 147 411 2823
Fragments arranged by size:
2823 2059 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 1178
Size: 30 165 30 708 30 5149
Fragments arranged by size:
5149 708 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2669 2759 2885 1186
Size: 1483 90 126 4413
Fragments arranged by size:
4413 1483 126 90
BseYI C'CCAG_C
Cuts at: 2201 2239 2745 3253 3316 5315 5811 2201
Size: 38 506 508 63 1999 496 2502
Fragments arranged by size:
2502 1999 508 506 496 63 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2347 2987 3332 3383 1163
Size: 573 611 640 345 51 3892
Fragments arranged by size:
3892 640 611 573 345 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3562 4323 5351 1540
Size: 275 186 1561 761 1028 2301
Fragments arranged by size:
2301 1561 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4173 4258 5329 4173
Size: 85 1071 4956
Fragments arranged by size:
4956 1071 85
BsmI GAATG_Cn'
Cuts at: 3623 3716 3623
Size: 93 6019
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2073 2169 2223 2486 2828 3158
Size: 399 261 22 96 54 263 342 330
Cuts at: 3158 3323 3431 4173 4258 5329 1391
Size: 165 108 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 330 263 261
165 108 96 85 54 22
BspEI T'CCGG_A
Cuts at: 5775 5775
Size: 6112
BspHI T'CATG_A
Cuts at: 2391 2691 4001 5731 2391
Size: 300 1310 1730 2772
Fragments arranged by size:
2772 1730 1310 300
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2031 2130 2267 2507 3125 3467 844
Size: 1187 99 137 240 618 342 3489
Fragments arranged by size:
3489 1187 618 342 240 137 99
BsrBI CCG'CTC
Cuts at: 1394 1823 3999 1394
Size: 429 2176 3507
Fragments arranged by size:
3507 2176 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4607 4781 65
Size: 1183 3359 174 1396
Fragments arranged by size:
3359 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2397 4756 4943 1700
Size: 110 587 2359 187 2869
Fragments arranged by size:
2869 2359 587 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6112
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6112
BssSI C'ACGA_G
Cuts at: 2548 2708 3208 4172 5184 2548
Size: 160 500 964 1012 3476
Fragments arranged by size:
3476 1012 964 500 160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6112
BstBI TT'CG_AA
Cuts at: 4916 4916
Size: 6112
BstEII G'GTnAC_C
Cuts at: 1184 2056 2176 2782 1184
Size: 872 120 606 4514
Fragments arranged by size:
4514 872 606 120
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 2695 3185 2587
Size: 108 490 5514
Fragments arranged by size:
5514 490 108
BstYI r'GATC_y
Cuts at: 1972 3442 3809 4194 4211 5663 5749 5761 6107
Size: 1470 367 385 17 1452 86 12 346
Cuts at: 6107 1972
Size: 1977
Fragments arranged by size:
1977 1470 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2757 3141 3381 513
Size: 552 939 753 384 240 3244
Fragments arranged by size:
3244 939 753 552 384 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2305 2548 487
Size: 683 56 1079 243 4051
Fragments arranged by size:
4051 1079 683 243 56
BtsI GCAGTG_nn'
Cuts at: 3628 4422 4442 3628
Size: 794 20 5298
Fragments arranged by size:
5298 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6112
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2410 2445 529
Size: 35 1846 35 4196
Fragments arranged by size:
4196 1846 35 35
DraI TTT'AAA
Cuts at: 3416 3416
Size: 6112
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5119 809
Size: 507 3803 1802
Fragments arranged by size:
3803 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2308 3033
Size: 54 1425 50 275 18 324 154 725
Cuts at: 3033 3138 3559 4448 6077 8
Size: 105 421 889 1629 43
Fragments arranged by size:
1629 1425 889 725 421 324 275 154
105 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3559 1537
Size: 275 1747 4090
Fragments arranged by size:
4090 1747 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2374 2805 3377 4042 1353
Size: 1021 431 572 665 3423
Fragments arranged by size:
3423 1021 665 572 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2584 4699 5073 5219 387
Size: 1064 138 995 2115 374 146 1280
Fragments arranged by size:
2115 1280 1064 995 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2651 2711 4175 4772 5559 5827
Size: 393 723 296 60 1464 597 787 268
Cuts at: 5827 1239
Size: 1524
Fragments arranged by size:
1524 1464 787 723 597 393 296 268
60
EcoNI CCTnn'n_nnAGG
Cuts at: 3170 3170
Size: 6112
EcoO109I rG'GnC_Cy
Cuts at: 2166 2825 3097 3114 2166
Size: 659 272 17 5164
Fragments arranged by size:
5164 659 272 17
EcoRI G'AATT_C
Cuts at: 3421 3799 3421
Size: 378 5734
EcoRV GAT'ATC
Cuts at: 2520 2520
Size: 6112
FspI TGC'GCA
Cuts at: 4618 4618
Size: 6112
HaeII r_GCGC'y
Cuts at: 1678 3288 3338 5259 1678
Size: 1610 50 1921 2531
Fragments arranged by size:
2531 1921 1610 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 4753 4785 4827
Size: 32 135 32 779 32 2370 32 42
Cuts at: 4827 4859 1373
Size: 32 2626
Fragments arranged by size:
2626 2370 779 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2735 2935 3358 3456 3702 669
Size: 988 281 797 200 423 98 246 3079
Fragments arranged by size:
3079 988 797 423 281 246 200 98
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6112
HpaI GTT'AAC
Cuts at: 3702 3702
Size: 6112
KpnI G_GTAC'C
Cuts at: 2122 3437 2122
Size: 1315 4797
MfeI C'AATT_G
Cuts at: 3711 3711
Size: 6112
MluI A'CGCG_T
Cuts at: 5988 5988
Size: 6112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5226 5410 1305
Size: 3921 184 2007
Fragments arranged by size:
3921 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 3035 3140 10
Size: 54 1425 343 324 879 105 2982
Fragments arranged by size:
2982 1425 879 343 324 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2693 3140 3146 3183 4070 4429 4588
Size: 648 1527 447 6 37 887 359 159
Cuts at: 4588 518
Size: 2042
Fragments arranged by size:
2042 1527 887 648 447 359 159 37
6
MspA1I CmG'CkG
Cuts at: 1606 2152 2205 2448 3225 3316 3383 3528 4205
Size: 546 53 243 777 91 67 145 677
Cuts at: 4205 5353 5598 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 777 677 546 245 243 145
91 67 53
NaeI GCC'GGC
Cuts at: 2399 2399
Size: 6112
NcoI C'CATG_G
Cuts at: 513 1065 2004 3141 513
Size: 552 939 1137 3484
Fragments arranged by size:
3484 1137 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6112
NgoMIV G'CCGG_C
Cuts at: 2397 2397
Size: 6112
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6112
NotI GC'GGCC_GC
Cuts at: 3559 3559
Size: 6112
NspI r_CATG'y
Cuts at: 1132 1229 2956 3470 5015 1132
Size: 97 1727 514 1545 2229
Fragments arranged by size:
2229 1727 1545 514 97
PasI CC'CwG_GG
Cuts at: 1348 2479 1348
Size: 1131 4981
PciI A'CATG_T
Cuts at: 5011 5011
Size: 6112
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2745 1108
Size: 1637 4475
PmeI GTTT'AAAC
Cuts at: 3416 3416
Size: 6112
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4181 4213 5694 5726 4181
Size: 32 1481 32 4567
Fragments arranged by size:
4567 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3097 3114 3097
Size: 17 6095
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6112
PsiI TTA'TAA
Cuts at: 3682 3682
Size: 6112
PspOMI G'GGCC_C
Cuts at: 2165 2824 2165
Size: 659 5453
PstI C_TGCA'G
Cuts at: 830 2833 3464 830
Size: 2003 631 3478
Fragments arranged by size:
3478 2003 631
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6112
PvuII CAG'CTG
Cuts at: 2152 2205 3316 2152
Size: 53 1111 4948
Fragments arranged by size:
4948 1111 53
SacII CC_GC'GG
Cuts at: 3384 3384
Size: 6112
SalI G'TCGA_C
Cuts at: 1936 3454 1936
Size: 1518 4594
SapI GCTCTTCn'nnn_
Cuts at: 3377 3377
Size: 6112
SbfI CC_TGCA'GG
Cuts at: 2833 3464 2833
Size: 631 5481
ScaI AGT'ACT
Cuts at: 4360 6072 4360
Size: 1712 4400
SexAI A'CCwGG_T
Cuts at: 2778 2778
Size: 6112
SfcI C'TryA_G
Cuts at: 826 1045 2829 3460 4595 5276 5467 826
Size: 219 1784 631 1135 681 191 1471
Fragments arranged by size:
1784 1471 1135 681 631 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6112
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6112
SmaI CCC'GGG
Cuts at: 1872 3439 1872
Size: 1567 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4216 5117 5379 820
Size: 197 926 2273 901 262 1553
Fragments arranged by size:
2273 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3903 493
Size: 3410 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6112
SphI G_CATG'C
Cuts at: 1132 2956 3470 1132
Size: 1824 514 3774
Fragments arranged by size:
3774 1824 514
SspI AAT'ATT
Cuts at: 5 52 4036 5
Size: 47 3984 2081
Fragments arranged by size:
3984 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6112
StyI C'CwwG_G
Cuts at: 513 1065 2004 3141 3551 513
Size: 552 939 1137 410 3074
Fragments arranged by size:
3074 1137 939 552 410
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2044 2287 2423 3202 4139 4156 4309
Size: 1255 464 243 136 779 937 17 153
Cuts at: 4309 4494 4939 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 937 779 464 445 243 185
153 136 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4358 6070 96
Size: 274 80 33 51 1443 2381 1712 138
Fragments arranged by size:
2381 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6072 6104 6072
Size: 32 6080
Tth111I GACn'n_nGTC
Cuts at: 1316 2780 1316
Size: 1464 4648
XbaI T'CTAG_A
Cuts at: 2866 3448 2866
Size: 582 5530
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2428
Size: 6112
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6112
XmaI C'CCGG_G
Cuts at: 1870 3437 1870
Size: 1567 4545
XmnI GAAnn'nnTTC
Cuts at: 4241 4241
Size: 6112
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3920 276
Size: 53 83 186 936 2386 2468
Fragments arranged by size:
2468 2386 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SanDI SfiI SfoI SrfI SwaI