Restriction map for
pFN21AE6457
(Circular) (Six-base) MAPSORT of: pFN21AE6457.seq Check: 4201 from: 1 to: 6133
pFN21AE6457 6133 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 2886 3454 2886
Size: 568 5565
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3212 4196 5580 5848 1632
Size: 1580 984 1384 268 1917
Fragments arranged by size:
1917 1580 1384 984 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 3184 5032 6009 1203
Size: 1981 1848 977 1327
Fragments arranged by size:
1981 1848 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 2299 4862 2299
Size: 2563 3570
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 3876 5448 3876
Size: 1572 4561
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2179 2258 2275 2570 2818 3293 3442 3668
Size: 686 79 17 295 248 475 149 226
Cuts at: 3668 3820 5929 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 686 475 295 248 226 152
149 79 17
AseI AT'TA_AT
Cuts at: 160 1032 2483 4687 160
Size: 872 1451 2204 1606
Fragments arranged by size:
2204 1606 1451 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2886 3454 4909 618
Size: 325 217 627 1099 568 1455 1842
Fragments arranged by size:
1842 1455 1099 627 568 325 217
BanII G_rGCy'C
Cuts at: 1391 3452 1391
Size: 2061 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2202 928
Size: 971 303 4859
Fragments arranged by size:
4859 971 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 4324 4358 1903
Size: 34 183 34 2170 34 3678
Fragments arranged by size:
3678 2170 183 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2361 2920 4025 5235 2361
Size: 559 1105 1210 3259
Fragments arranged by size:
3259 1210 1105 559
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2022 6128 2022
Size: 4106 2027
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 3263 4194 5350 1790
Size: 1473 931 1156 2573
Fragments arranged by size:
2573 1473 1156 931
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3864 4822 442
Size: 416 53 83 399 24 2447 958 1753
Fragments arranged by size:
2447 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4793 1239
Size: 3554 2579
Bpu10I CC'TnA_GC
Cuts at: 2431 2609 2431
Size: 178 5955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4258 5123 5421 4258
Size: 865 298 4970
Fragments arranged by size:
4970 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 2027 6127 2027
Size: 4100 2033
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4322 4957 275
Size: 53 83 186 936 2407 382 635 1451
Fragments arranged by size:
2407 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2012 4566 4964 5238 5385 5796 2012
Size: 2554 398 274 147 411 2349
Fragments arranged by size:
2554 2349 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2054 2084 2402 2432 1178
Size: 30 165 30 651 30 318 30 4879
Fragments arranged by size:
4879 651 318 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2322 3051 3285 3375 1186
Size: 1136 729 234 90 3944
Fragments arranged by size:
3944 1136 729 234 90
BseYI C'CCAG_C
Cuts at: 2454 5336 5832 2454
Size: 2882 496 2755
Fragments arranged by size:
2882 2755 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2407 1163
Size: 573 671 4889
Fragments arranged by size:
4889 671 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3277 4194 4279 5350 3277
Size: 917 85 1071 4060
Fragments arranged by size:
4060 1071 917 85
BsmI GAATG_Cn'
Cuts at: 2242 3644 3737 2242
Size: 1402 93 4638
Fragments arranged by size:
4638 1402 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3263 3277 3452 4194 4279 5350 1391
Size: 399 1473 14 175 742 85 1071 2174
Fragments arranged by size:
2174 1473 1071 742 399 175 85 14
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2647 2967 3488 844
Size: 1803 320 521 3489
Fragments arranged by size:
3489 1803 521 320
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2447 4628 4802 65
Size: 1183 1199 2181 174 1396
Fragments arranged by size:
2181 1396 1199 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2421 794
Size: 1627 4506
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2022 3045 3463 3830 4215 4232 5684 5770
Size: 50 1023 418 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1023 418 385 367 346 86
50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2967 513
Size: 552 1902 3679
Fragments arranged by size:
3679 1902 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5394
Fragments arranged by size:
5394 683 56
BtsI GCAGTG_nn'
Cuts at: 2745 3649 4443 4463 2745
Size: 904 794 20 4415
Fragments arranged by size:
4415 904 794 20
ClaI AT'CG_AT
Cuts at: 1554 2871 1554
Size: 1317 4816
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 2569 3437 2569
Size: 868 5265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2799 3580 4469
Size: 54 1425 50 275 18 969 781 889
Cuts at: 4469 6098 8
Size: 1629 43
Fragments arranged by size:
1629 1425 969 889 781 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2310 2382 3082 4063 1353
Size: 957 72 700 981 3423
Fragments arranged by size:
3423 981 957 700 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3212 4196 4793 5580 5848 1239
Size: 393 1580 984 597 787 268 1524
Fragments arranged by size:
1580 1524 984 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 5280 1678
Size: 3602 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2007 2039 2285 2317 4774
Size: 32 135 32 435 32 246 32 2457
Cuts at: 4774 4806 4848 4880 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2457 435 246 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2378 3477 3723 669
Size: 988 281 440 1099 246 3079
Fragments arranged by size:
3079 1099 988 440 281 246
HindIII A'AGCT_T
Cuts at: 748 3224 748
Size: 2476 3657
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 2890 3458 2890
Size: 568 5565
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3105 3399 5247 5431 1305
Size: 1800 294 1848 184 2007
Fragments arranged by size:
2007 1848 1800 294 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4311
Fragments arranged by size:
4311 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4091 4450 4609 518
Size: 648 2925 359 159 2042
Fragments arranged by size:
2925 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3549 4226 5374 5619 1606
Size: 1943 677 1148 245 2120
Fragments arranged by size:
2120 1943 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2967 513
Size: 552 1902 3679
Fragments arranged by size:
3679 1902 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2430 2462 2922 3005 3188 3239 3491
Size: 97 1201 32 460 83 183 51 252
Cuts at: 3491 5036 1132
Size: 1545 2229
Fragments arranged by size:
2229 1545 1201 460 252 183 97 83
51 32
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 3184 5032 3184
Size: 1848 4285
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3315 1108
Size: 2207 3926
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 2679 3165 3703 2679
Size: 486 538 5109
Fragments arranged by size:
5109 538 486
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2454 2486 2454
Size: 32 6101
PstI C_TGCA'G
Cuts at: 830 3485 830
Size: 2655 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SfcI C'TryA_G
Cuts at: 826 1045 2046 2173 3481 4616 5297 5488 826
Size: 219 1001 127 1308 1135 681 191 1471
Fragments arranged by size:
1471 1308 1135 1001 681 219 191 127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4237 5138 5400 820
Size: 197 926 2294 901 262 1553
Fragments arranged by size:
2294 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 2922 3005 3491 1132
Size: 1790 83 486 3774
Fragments arranged by size:
3774 1790 486 83
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2161 2345 2578 2967 3572 513
Size: 552 1096 184 233 389 605 3074
Fragments arranged by size:
3074 1096 605 552 389 233 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2781 4160 4177 4330 4515 4960 325
Size: 1255 1201 1379 17 153 185 445 1498
Fragments arranged by size:
1498 1379 1255 1201 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4379 6091 96
Size: 274 80 33 51 1443 2402 1712 138
Fragments arranged by size:
2402 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6093 6125 6093
Size: 32 6101
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6133
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2752 2752
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI