Restriction map for
pFN21AE6456
(Circular) (Six-base) MAPSORT of: pFN21AE6456.seq Check: 3294 from: 1 to: 6364
pFN21AE6456 6364 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3240 3240
Size: 6364
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4174 278
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3588 3707 1937
Size: 1651 119 4594
Fragments arranged by size:
4594 1651 119
Acc65I G'GTAC_C
Cuts at: 3685 3685
Size: 6364
AclI AA'CG_TT
Cuts at: 1563 4491 4864 1563
Size: 2928 373 3063
Fragments arranged by size:
3063 2928 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3119 4427 5811 6079 1632
Size: 1487 1308 1384 268 1917
Fragments arranged by size:
1917 1487 1384 1308 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6167
AflIII A'CryG_T
Cuts at: 1203 2561 5263 6240 1203
Size: 1358 2702 977 1327
Fragments arranged by size:
2702 1358 1327 977
AgeI A'CCGG_T
Cuts at: 5195 5195
Size: 6364
AhdI GACnn_n'nnGTC
Cuts at: 2910 5093 2910
Size: 2183 4181
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3359 1184
Size: 2175 4189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2700 2732 2700
Size: 32 6332
AlwNI CAG_nnn'CTG
Cuts at: 2138 2802 3356 4107 5679 2138
Size: 664 554 751 1572 2823
Fragments arranged by size:
2823 1572 751 664 554
ApaI G_GGCC'C
Cuts at: 2024 2639 2660 2684 3271 2024
Size: 615 21 24 587 5117
Fragments arranged by size:
5117 615 587 24 21
ApaLI G'TGCA_C
Cuts at: 4421 5577 4421
Size: 1156 5208
ApoI r'AATT_y
Cuts at: 1493 2266 3673 3899 4051 6160 1493
Size: 773 1407 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 773 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4918 160
Size: 872 3886 1606
Fragments arranged by size:
3886 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
AvaI C'yCGr_G
Cuts at: 1870 1943 2696 2814 3611 3689 1870
Size: 73 753 118 797 78 4545
Fragments arranged by size:
4545 797 753 118 78 73
AvrII C'CTAG_G
Cuts at: 3326 3326
Size: 6364
BamHI G'GATC_C
Cuts at: 2894 3694 4061 2894
Size: 800 367 5197
Fragments arranged by size:
5197 800 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2042 3039 3442 3685 5140
Size: 325 217 627 255 997 403 243 1455
Cuts at: 5140 618
Size: 1842
Fragments arranged by size:
1842 1455 997 627 403 325 255 243
217
BanII G_rGCy'C
Cuts at: 1391 2024 2182 2384 2639 2660 2684 2849 2992
Size: 633 158 202 255 21 24 165 143
Cuts at: 2992 3271 3612 3683 1391
Size: 279 341 71 4072
Fragments arranged by size:
4072 633 341 279 255 202 165 158
143 71 24 21
BbeI G_GCGC'C
Cuts at: 3043 3043
Size: 6364
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2057 3526 928
Size: 971 158 1469 3766
Fragments arranged by size:
3766 1469 971 158
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4555 4589 1903
Size: 34 2618 34 3678
Fragments arranged by size:
3678 2618 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4256 5466 4256
Size: 1210 5154
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6364
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3527 4975 136
Size: 107 122 71 631 605 1855 1448 1525
Fragments arranged by size:
1855 1525 1448 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6359 6359
Size: 6364
BlpI GC'TnA_GC
Cuts at: 2076 3618 3781 2076
Size: 1542 163 4659
Fragments arranged by size:
4659 1542 163
Bme1580I G_kGCm'C
Cuts at: 1790 2024 2639 2660 2684 2755 3271 3309 3445
Size: 234 615 21 24 71 516 38 136
Cuts at: 3445 3470 4425 5581 1790
Size: 25 955 1156 2573
Fragments arranged by size:
2573 1156 955 615 516 234 136 71
38 25 24 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6364
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2018 2722 3194
Size: 416 53 83 399 24 601 704 472
Cuts at: 3194 3571 4095 5053 442
Size: 377 524 958 1753
Fragments arranged by size:
1753 958 704 601 524 472 416 399
377 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3281 5024 1239
Size: 2042 1743 2579
Fragments arranged by size:
2579 2042 1743
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2920 4489 5354 5652 2920
Size: 1569 865 298 3632
Fragments arranged by size:
3632 1569 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2846 5027 882
Size: 617 1347 2181 2219
Fragments arranged by size:
2219 2181 1347 617
BsaAI yAC'GTr
Cuts at: 493 1204 4155 493
Size: 711 2951 2702
Fragments arranged by size:
2951 2702 711
BsaBI GATnn'nnATC
Cuts at: 6358 6358
Size: 6364
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3040 4171 4553 5188
Size: 53 83 186 936 1507 1131 382 635
Cuts at: 5188 275
Size: 1451
Fragments arranged by size:
1507 1451 1131 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3082 4797 5195 5469 5616 6027 3082
Size: 1715 398 274 147 411 3419
Fragments arranged by size:
3419 1715 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2979 3009 1178
Size: 30 165 30 1576 30 4533
Fragments arranged by size:
4533 1576 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 3017 3101 1186
Size: 996 835 84 4449
Fragments arranged by size:
4449 996 835 84
BseYI C'CCAG_C
Cuts at: 2016 2442 2478 2490 2640 3070 3171 3252 3419
Size: 426 36 12 150 430 101 81 167
Cuts at: 3419 5567 6063 2016
Size: 2148 496 2317
Fragments arranged by size:
2317 2148 496 430 426 167 150 101
81 36 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2570 2672 3473 1163
Size: 573 834 102 801 4054
Fragments arranged by size:
4054 834 801 573 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3814 4575 5603 1540
Size: 275 186 1813 761 1028 2301
Fragments arranged by size:
2301 1813 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2929 4425 4510 5581 2929
Size: 1496 85 1071 3712
Fragments arranged by size:
3712 1496 1071 85
BsmI GAATG_Cn'
Cuts at: 3875 3968 3875
Size: 93 6271
BsmBI CGTCTCn'nnnn_
Cuts at: 2549 2549
Size: 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 2182 2384 2639 2660 2684 2755
Size: 399 234 158 202 255 21 24 71
Cuts at: 2755 2849 2929 2992 3271 3309 3445 3470 3612
Size: 94 80 63 279 38 136 25 142
Cuts at: 3612 3683 4425 4510 5581 1391
Size: 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 279 255 234 202
158 142 136 94 85 80 71 71
63 38 25 24 21
BspEI T'CCGG_A
Cuts at: 6027 6027
Size: 6364
BspHI T'CATG_A
Cuts at: 4253 5983 4253
Size: 1730 4634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2900 3240 3574 3719 844
Size: 2056 340 334 145 3489
Fragments arranged by size:
3489 2056 340 334 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2075 4251 1394
Size: 429 252 2176 3507
Fragments arranged by size:
3507 2176 429 252
BsrDI GCAATG_nn'
Cuts at: 65 1248 4859 5033 65
Size: 1183 3611 174 1396
Fragments arranged by size:
3611 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3339 3526 5008 5195 1700
Size: 110 1529 187 1482 187 2869
Fragments arranged by size:
2869 1529 1482 187 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6364
BssSI C'ACGA_G
Cuts at: 4424 5436 4424
Size: 1012 5352
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6364
BstBI TT'CG_AA
Cuts at: 5168 5168
Size: 6364
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6364
BstXI CCAn_nnnn'nTGG
Cuts at: 2943 3057 3406 2943
Size: 114 349 5901
Fragments arranged by size:
5901 349 114
BstYI r'GATC_y
Cuts at: 1972 2894 3694 4061 4446 4463 5915 6001 6013
Size: 922 800 367 385 17 1452 86 12
Cuts at: 6013 6359 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 922 800 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2118 3135 2118
Size: 1017 5347
BtgI C'CryG_G
Cuts at: 513 1065 2936 513
Size: 552 1871 3941
Fragments arranged by size:
3941 1871 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2020 2438 487
Size: 683 56 794 418 4413
Fragments arranged by size:
4413 794 683 418 56
BtsI GCAGTG_nn'
Cuts at: 2223 2233 3025 3880 4674 4694 2223
Size: 10 792 855 794 20 3893
Fragments arranged by size:
3893 855 794 792 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6364
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6329
DraI TTT'AAA
Cuts at: 3668 3668
Size: 6364
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2216 3584 5371 809
Size: 507 900 1368 1787 1802
Fragments arranged by size:
1802 1787 1368 900 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3480 3636 3811
Size: 54 1425 50 275 18 1650 156 175
Cuts at: 3811 4700 6329 8
Size: 889 1629 43
Fragments arranged by size:
1650 1629 1425 889 275 175 156 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3811 1537
Size: 275 1999 4090
Fragments arranged by size:
4090 1999 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 4294 1353
Size: 966 1975 3423
Fragments arranged by size:
3423 1975 966
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3000 4951 5325 5471 387
Size: 1064 138 1411 1951 374 146 1280
Fragments arranged by size:
1951 1411 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3119 3281 4427 5024 5811 6079 1239
Size: 393 1487 162 1146 597 787 268 1524
Fragments arranged by size:
1524 1487 1146 787 597 393 268 162
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 6364
EcoO109I rG'GnC_Cy
Cuts at: 2020 2218 2358 2520 2635 2636 2656 2657 2680
Size: 198 140 162 115 1 20 1 23
Cuts at: 2680 2681 2912 3046 3094 3323 3330 3430 3515
Size: 1 231 134 48 229 7 100 85
Cuts at: 3515 2020
Size: 4869
Fragments arranged by size:
4869 231 229 198 162 140 134 115
100 85 48 23 20 7 1 1
1
EcoRI G'AATT_C
Cuts at: 2266 3673 4051 2266
Size: 1407 378 4579
Fragments arranged by size:
4579 1407 378
FspI TGC'GCA
Cuts at: 4870 4870
Size: 6364
HaeII r_GCGC'y
Cuts at: 1678 3043 5511 1678
Size: 1365 2468 2531
Fragments arranged by size:
2531 2468 1365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2169 2201 2896 2928 3270
Size: 32 135 32 597 32 695 32 342
Cuts at: 3270 3302 3419 3451 5005 5037 5079 5111 1373
Size: 32 117 32 1554 32 42 32 2626
Fragments arranged by size:
2626 1554 695 597 342 135 117 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3708 3954 669
Size: 988 281 1770 246 3079
Fragments arranged by size:
3079 1770 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6364
HpaI GTT'AAC
Cuts at: 3954 3954
Size: 6364
KasI G'GCGC_C
Cuts at: 3039 3039
Size: 6364
KpnI G_GTAC'C
Cuts at: 3689 3689
Size: 6364
MfeI C'AATT_G
Cuts at: 3963 3963
Size: 6364
MluI A'CGCG_T
Cuts at: 6240 6240
Size: 6364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2628 5478 5662 1305
Size: 1323 2850 184 2007
Fragments arranged by size:
2850 2007 1323 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3638 10
Size: 54 1425 343 1806 2736
Fragments arranged by size:
2736 1806 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4322 4681 4840 518
Size: 648 3156 359 159 2042
Fragments arranged by size:
3156 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2193 2584 2789 2880 2974 3423 3660 3780
Size: 587 391 205 91 94 449 237 120
Cuts at: 3780 4457 5605 5850 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 587 449 391 245 237
205 120 94 91
NaeI GCC'GGC
Cuts at: 3341 3528 3341
Size: 187 6177
NarI GG'CG_CC
Cuts at: 3040 3040
Size: 6364
NcoI C'CATG_G
Cuts at: 513 1065 2936 513
Size: 552 1871 3941
Fragments arranged by size:
3941 1871 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6364
NgoMIV G'CCGG_C
Cuts at: 3339 3526 3339
Size: 187 6177
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6364
NotI GC'GGCC_GC
Cuts at: 3811 3811
Size: 6364
NspI r_CATG'y
Cuts at: 1132 1229 2565 3350 3722 5267 1132
Size: 97 1336 785 372 1545 2229
Fragments arranged by size:
2229 1545 1336 785 372 97
PasI CC'CwG_GG
Cuts at: 1348 2315 2355 2943 1348
Size: 967 40 588 4769
Fragments arranged by size:
4769 967 588 40
PciI A'CATG_T
Cuts at: 2561 5263 2561
Size: 2702 3662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2490 1108
Size: 902 480 4982
Fragments arranged by size:
4982 902 480
PmeI GTTT'AAAC
Cuts at: 3668 3668
Size: 6364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4433 4465 5946 5978 4433
Size: 32 1481 32 4819
Fragments arranged by size:
4819 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2218 2520 2912 2218
Size: 302 392 5670
Fragments arranged by size:
5670 392 302
PshAI GACnn'nnGTC
Cuts at: 1613 2599 1613
Size: 986 5378
PsiI TTA'TAA
Cuts at: 3934 3934
Size: 6364
PspOMI G'GGCC_C
Cuts at: 2020 2635 2656 2680 3267 2020
Size: 615 21 24 587 5117
Fragments arranged by size:
5117 615 587 24 21
PspXI vC'TCGA_Gb
Cuts at: 2696 2814 2696
Size: 118 6246
PstI C_TGCA'G
Cuts at: 830 2192 2298 2349 2403 2811 3338 3716 830
Size: 1362 106 51 54 408 527 378 3478
Fragments arranged by size:
3478 1362 527 408 378 106 54 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6364
PvuII CAG'CTG
Cuts at: 2584 2789 2880 2974 3423 3660 2584
Size: 205 91 94 449 237 5288
Fragments arranged by size:
5288 449 237 205 94 91
SalI G'TCGA_C
Cuts at: 1936 3706 1936
Size: 1770 4594
SanDI GG'GwC_CC
Cuts at: 2520 2520
Size: 6364
SbfI CC_TGCA'GG
Cuts at: 3716 3716
Size: 6364
ScaI AGT'ACT
Cuts at: 4612 6324 4612
Size: 1712 4652
SfcI C'TryA_G
Cuts at: 826 1045 2188 2294 2345 2371 2399 2807 3334
Size: 219 1143 106 51 26 28 408 527
Cuts at: 3334 3712 4847 5528 5719 826
Size: 378 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 681 527 408 378 219
191 106 51 28 26
SfoI GGC'GCC
Cuts at: 3041 3041
Size: 6364
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6364
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6364
SmaI CCC'GGG
Cuts at: 1872 3691 1872
Size: 1819 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2696 2814 2899 4468 5369 5631
Size: 197 926 753 118 85 1569 901 262
Cuts at: 5631 820
Size: 1553
Fragments arranged by size:
1569 1553 926 901 753 262 197 118
85
SnaBI TAC'GTA
Cuts at: 493 4155 493
Size: 3662 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6364
SphI G_CATG'C
Cuts at: 1132 3350 3722 1132
Size: 2218 372 3774
Fragments arranged by size:
3774 2218 372
SspI AAT'ATT
Cuts at: 5 52 4288 5
Size: 47 4236 2081
Fragments arranged by size:
4236 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6364
StyI C'CwwG_G
Cuts at: 513 1065 2936 3326 3511 3803 513
Size: 552 1871 390 185 292 3074
Fragments arranged by size:
3074 1871 552 390 292 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3398 4391 4408 4561 4746 5191
Size: 1255 1563 255 993 17 153 185 445
Cuts at: 5191 325
Size: 1498
Fragments arranged by size:
1563 1498 1255 993 445 255 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4610 6322 96
Size: 274 80 33 51 1443 2633 1712 138
Fragments arranged by size:
2633 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2614 2646 2979 3011 6324 6356 2614
Size: 32 333 32 3313 32 2622
Fragments arranged by size:
3313 2622 333 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3563 1316
Size: 2247 4117
XbaI T'CTAG_A
Cuts at: 3700 3700
Size: 6364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2053 3032 3236 2053
Size: 979 204 5181
Fragments arranged by size:
5181 979 204
XhoI C'TCGA_G
Cuts at: 1943 2696 2814 1943
Size: 753 118 5493
Fragments arranged by size:
5493 753 118
XmaI C'CCGG_G
Cuts at: 1870 3689 1870
Size: 1819 4545
XmnI GAAnn'nnTTC
Cuts at: 4493 4493
Size: 6364
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4172 276
Size: 53 83 186 936 2638 2468
Fragments arranged by size:
2638 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PsrI RsrII SacI SacII
SapI SexAI SfiI SrfI SwaI