Restriction map for
pFN21AE6451
(Circular) (Six-base) MAPSORT of: pFN21AE6451.seq Check: 2935 from: 1 to: 6217
pFN21AE6451 6217 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3402 4280 5664 5932 1632
Size: 1770 878 1384 268 1917
Fragments arranged by size:
1917 1770 1384 878 268
AfeI AGC'GCT
Cuts at: 2513 2513
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2503 2506 3344 3960 5532 2503
Size: 3 838 616 1572 3188
Fragments arranged by size:
3188 1572 838 616 3
ApaI G_GGCC'C
Cuts at: 2039 2222 2609 2039
Size: 183 387 5647
Fragments arranged by size:
5647 387 183
ApaLI G'TGCA_C
Cuts at: 2746 4274 5430 2746
Size: 1528 1156 3533
Fragments arranged by size:
3533 1528 1156
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2909 3123 2909
Size: 214 6003
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2597 2798 3456 3542 1870
Size: 73 654 201 658 86 4545
Fragments arranged by size:
4545 658 654 201 86 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2724 2924 3035 3538
Size: 325 217 627 285 652 200 111 503
Cuts at: 3538 4993 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 652 627 503 325 285 217
200 111
BanII G_rGCy'C
Cuts at: 1391 2039 2222 2337 2553 2609 2805 3000 3399
Size: 648 183 115 216 56 196 195 399
Cuts at: 3399 3468 3536 1391
Size: 69 68 4072
Fragments arranged by size:
4072 648 399 216 196 195 183 115
69 68 56
BbeI G_GCGC'C
Cuts at: 2076 2728 2928 3039 2076
Size: 652 200 111 5254
Fragments arranged by size:
5254 652 200 111
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 3271 928
Size: 971 220 1152 3874
Fragments arranged by size:
3874 1152 971 220
BbvCI CC'TCA_GC
Cuts at: 3375 3375
Size: 6217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2576 2599 2722
Size: 107 122 71 631 605 904 23 123
Cuts at: 2722 2987 4828 136
Size: 265 1841 1525
Fragments arranged by size:
1841 1525 904 631 605 265 123 122
107 71 23
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3449 3634 3449
Size: 185 6032
Bme1580I G_kGCm'C
Cuts at: 1790 2039 2222 2609 2750 2856 4278 5434 1790
Size: 249 183 387 141 106 1422 1156 2573
Fragments arranged by size:
2573 1422 1156 387 249 183 141 106
BmgBI CAC'GTC
Cuts at: 1318 2959 1318
Size: 1641 4576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3132 3948 4906
Size: 416 53 83 399 24 1715 816 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1753 1715 958 816 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2201 1056
Size: 1145 5072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2351 2528 3452 3461 4877 1239
Size: 1112 177 924 9 1416 2579
Fragments arranged by size:
2579 1416 1112 924 177 9
Bpu10I CC'TnA_GC
Cuts at: 3008 3375 3008
Size: 367 5850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4342 5207 5505 4342
Size: 865 298 5054
Fragments arranged by size:
5054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2157 2725 2865
Size: 53 83 186 936 540 84 568 140
Cuts at: 2865 2925 3036 4024 4406 5041 275
Size: 60 111 988 382 635 1451
Fragments arranged by size:
1451 988 936 635 568 540 382 186
140 111 84 83 60 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5992
Fragments arranged by size:
5992 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6217
BseYI C'CCAG_C
Cuts at: 2052 2058 2068 2348 2404 3145 3272 5420 5916
Size: 6 10 280 56 741 127 2148 496
Cuts at: 5916 2052
Size: 2353
Fragments arranged by size:
2353 2148 741 496 280 127 56 10
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2543 1163
Size: 573 807 4837
Fragments arranged by size:
4837 807 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2615 2638 2755 2954 3205 3667
Size: 275 186 614 23 117 199 251 462
Cuts at: 3667 4428 5456 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 614 462 275 251 199
186 117 23
BsiHKAI G_wGCw'C
Cuts at: 2337 2358 2750 3108 3183 3450 4278 4363 5434
Size: 21 392 358 75 267 828 85 1071
Cuts at: 5434 2337
Size: 3120
Fragments arranged by size:
3120 1071 828 392 358 267 85 75
21
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2039 2222 2337 2358 2553 2609 2750
Size: 399 249 183 115 21 195 56 141
Cuts at: 2750 2805 2856 3000 3108 3183 3399 3450 3468
Size: 55 51 144 108 75 216 51 18
Cuts at: 3468 3536 4278 4363 5434 1391
Size: 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 249 216 195 183
144 141 115 108 85 75 68 56
55 51 51 21 18
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 2583 4106 5836 2583
Size: 1523 1730 2964
Fragments arranged by size:
2964 1730 1523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2422 2491 4104 1394
Size: 429 599 69 1613 3507
Fragments arranged by size:
3507 1613 599 429 69
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 2159 2630 2721 3200 3251 4861
Size: 110 280 69 471 91 479 51 1610
Cuts at: 4861 5048 1700
Size: 187 2869
Fragments arranged by size:
2869 1610 479 471 280 187 110 91
69 51
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 2136 2909 3021 3023 3087 3123 1925
Size: 211 773 112 2 64 36 5019
Fragments arranged by size:
5019 773 211 112 64 36 2
BssSI C'ACGA_G
Cuts at: 2237 3161 4277 5289 2237
Size: 924 1116 1012 3165
Fragments arranged by size:
3165 1116 1012 924
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 2086 1184
Size: 902 5315
BstXI CCAn_nnnn'nTGG
Cuts at: 2446 2824 3145 2446
Size: 378 321 5518
Fragments arranged by size:
5518 378 321
BstYI r'GATC_y
Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212
Size: 1575 367 385 17 1452 86 12 346
Cuts at: 6212 1972
Size: 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2292 3171 513
Size: 552 939 288 879 3559
Fragments arranged by size:
3559 939 879 552 288
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 2748 3733 4527 4547 2560
Size: 188 985 794 20 4230
Fragments arranged by size:
4230 985 794 188 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2436 2494 2951
Size: 54 1425 50 275 18 606 58 457
Cuts at: 2951 2988 3202 3249 3664 4553 6182 8
Size: 37 214 47 415 889 1629 43
Fragments arranged by size:
1629 1425 889 606 457 415 275 214
58 54 50 47 43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 2951 3202 3664 1537
Size: 275 1139 251 462 4090
Fragments arranged by size:
4090 1139 462 275 251
EarI CTCTTCn'nnn_
Cuts at: 1353 2810 4147 1353
Size: 1457 1337 3423
Fragments arranged by size:
3423 1457 1337
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2678 3366 4804 5178 5324 387
Size: 1064 138 1089 688 1438 374 146 1280
Fragments arranged by size:
1438 1280 1089 1064 688 374 146 138
EcoICRI GAG'CTC
Cuts at: 2335 2335
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2351 2528 3402 3452 3461 4280 4877
Size: 393 719 177 874 50 9 819 597
Cuts at: 4877 5664 5932 1239
Size: 787 268 1524
Fragments arranged by size:
1524 874 819 787 719 597 393 268
177 50 9
EcoO109I rG'GnC_Cy
Cuts at: 2035 2036 2219 3436 2035
Size: 1 183 1217 4816
Fragments arranged by size:
4816 1217 183 1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2013 2076 2515 2631 2728 2798 2928 3039
Size: 335 63 439 116 97 70 130 111
Cuts at: 3039 3456 5364 1678
Size: 417 1908 2531
Fragments arranged by size:
2531 1908 439 417 335 130 116 111
97 70 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373
Size: 32 135 32 3286 32 42 32 2626
Fragments arranged by size:
3286 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2072 2724 2924 3035 2072
Size: 652 200 111 5254
Fragments arranged by size:
5254 652 200 111
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2438 2990 10
Size: 54 1425 343 606 552 3237
Fragments arranged by size:
3237 1425 606 552 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 4175 4534 4693 518
Size: 648 1071 1938 359 159 2042
Fragments arranged by size:
2042 1938 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2294 2348 2503 2689 3173 3633 4310 5458
Size: 688 54 155 186 484 460 677 1148
Cuts at: 5458 5703 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 688 677 484 460 245 186
155 54
NaeI GCC'GGC
Cuts at: 2161 2632 2723 3202 3253 2161
Size: 471 91 479 51 5125
Fragments arranged by size:
5125 479 471 91 51
NarI GG'CG_CC
Cuts at: 2073 2725 2925 3036 2073
Size: 652 200 111 5254
Fragments arranged by size:
5254 652 200 111
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4726
Fragments arranged by size:
4726 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2159 2630 2721 3200 3251 2159
Size: 471 91 479 51 5125
Fragments arranged by size:
5125 479 471 91 51
NheI G'CTAG_C
Cuts at: 1052 2197 1052
Size: 1145 5072
NotI GC'GGCC_GC
Cuts at: 2951 3664 2951
Size: 713 5504
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2706 1348
Size: 1358 4859
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PfoI T'CCnGG_A
Cuts at: 2328 2328
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2035 2218 2605 2035
Size: 183 387 5647
Fragments arranged by size:
5647 387 183
PstI C_TGCA'G
Cuts at: 830 2688 3096 3569 830
Size: 1858 408 473 3478
Fragments arranged by size:
3478 1858 473 408
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2503 2689 2503
Size: 186 6031
RsrII CG'GwC_CG
Cuts at: 3188 3188
Size: 6217
SacI G_AGCT'C
Cuts at: 2337 2337
Size: 6217
SacII CC_GC'GG
Cuts at: 2295 3174 2295
Size: 879 5338
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2810 2810
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2164 2684 3092 3513 3565 4700 5381
Size: 219 1119 520 408 421 52 1135 681
Cuts at: 5381 5572 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1119 681 520 421 408 219
191 52
SfiI GGCCn_nnn'nGGCC
Cuts at: 2987 2987
Size: 6217
SfoI GGC'GCC
Cuts at: 2074 2726 2926 3037 2074
Size: 652 200 111 5254
Fragments arranged by size:
5254 652 200 111
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 2630 2721 1700
Size: 930 91 5196
Fragments arranged by size:
5196 930 91
SmaI CCC'GGG
Cuts at: 1872 2599 3458 3544 1872
Size: 727 859 86 4545
Fragments arranged by size:
4545 859 727 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4321 5222 5484 820
Size: 197 926 2378 901 262 1553
Fragments arranged by size:
2378 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SrfI GCCC'GGGC
Cuts at: 2599 2599
Size: 6217
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2364 1517
Size: 847 5370
StyI C'CwwG_G
Cuts at: 513 1065 2004 2838 3656 513
Size: 552 939 834 818 3074
Fragments arranged by size:
3074 939 834 818 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2170 4244 4261 4414 4599 5044
Size: 1255 470 120 2074 17 153 185 445
Cuts at: 5044 325
Size: 1498
Fragments arranged by size:
2074 1498 1255 470 445 185 153 120
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 4463 6175
Size: 274 80 33 51 1443 435 2051 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
2051 1712 1443 435 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2049 2326 2788 2049
Size: 277 462 5478
Fragments arranged by size:
5478 462 277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 2597 3456 3542 1870
Size: 727 859 86 4545
Fragments arranged by size:
4545 859 727 86
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI SanDI
SexAI SwaI