Restriction map for
pFN21AE6450
(Circular) (Six-base) MAPSORT of: pFN21AE6450.seq Check: 9662 from: 1 to: 6286
pFN21AE6450 6286 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 2560 3607 2560
Size: 1047 5239
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2018 4349 5733 6001 1632
Size: 386 2331 1384 268 1917
Fragments arranged by size:
2331 1917 1384 386 268
AfeI AGC'GCT
Cuts at: 3280 3280
Size: 6286
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 2667 5185 6162 1203
Size: 1464 2518 977 1327
Fragments arranged by size:
2518 1464 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 2614 5015 2614
Size: 2401 3885
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6286
AlwNI CAG_nnn'CTG
Cuts at: 4029 5601 4029
Size: 1572 4714
ApaLI G'TGCA_C
Cuts at: 2175 4343 5499 2175
Size: 2168 1156 2962
Fragments arranged by size:
2962 2168 1156
ApoI r'AATT_y
Cuts at: 1493 2496 2638 2760 2792 2879 3339 3551 3595
Size: 1003 142 122 32 87 460 212 44
Cuts at: 3595 3821 3973 6082 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1003 460 226 212 152 142
122 87 44 32
AseI AT'TA_AT
Cuts at: 160 1032 3297 4840 160
Size: 872 2265 1543 1606
Fragments arranged by size:
2265 1606 1543 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2230 2560 3607 5062 618
Size: 325 217 627 443 330 1047 1455 1842
Fragments arranged by size:
1842 1455 1047 627 443 330 325 217
BanII G_rGCy'C
Cuts at: 1391 2133 2894 3605 1391
Size: 742 761 711 4072
Fragments arranged by size:
4072 761 742 711
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2728 3373 928
Size: 971 829 645 3841
Fragments arranged by size:
3841 971 829 645
BbvCI CC'TCA_GC
Cuts at: 2974 2974
Size: 6286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3060 4178 5388 3060
Size: 1118 1210 3958
Fragments arranged by size:
3958 1210 1118
BclI T'GATC_A
Cuts at: 1548 3190 3349 1548
Size: 1642 159 4485
Fragments arranged by size:
4485 1642 159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2376 6281 2376
Size: 3905 2381
BlpI GC'TnA_GC
Cuts at: 3275 3703 3275
Size: 428 5858
Bme1580I G_kGCm'C
Cuts at: 1790 2179 2235 4347 5503 1790
Size: 389 56 2112 1156 2573
Fragments arranged by size:
2573 2112 1156 389 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4017 4975 442
Size: 416 53 83 399 24 2600 958 1753
Fragments arranged by size:
2600 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2541 2541
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2511 4946 1239
Size: 1272 2435 2579
Fragments arranged by size:
2579 2435 1272
Bpu10I CC'TnA_GC
Cuts at: 2893 2974 2893
Size: 81 6205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2536 4411 5276 5574 2536
Size: 1875 865 298 3248
Fragments arranged by size:
3248 1875 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4949 882
Size: 617 3450 2219
Fragments arranged by size:
3450 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3219 3249 1178
Size: 30 165 30 1816 30 4215
Fragments arranged by size:
4215 1816 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6286
BseYI C'CCAG_C
Cuts at: 5489 5985 5489
Size: 496 5790
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5713
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2179 2894 4347 4432 5503 2133
Size: 46 715 1453 85 1071 2916
Fragments arranged by size:
2916 1453 1071 715 85 46
BsmI GAATG_Cn'
Cuts at: 3325 3797 3890 3325
Size: 472 93 5721
Fragments arranged by size:
5721 472 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2133 2179 2235 2894 3605 4347 4432
Size: 399 343 46 56 659 711 742 85
Cuts at: 4432 5503 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 711 659 399 343 85
56 46
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2857 3641 844
Size: 2013 784 3489
Fragments arranged by size:
3489 2013 784
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3204 4781 4955 65
Size: 1183 1956 1577 174 1396
Fragments arranged by size:
1956 1577 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6286
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2376 3135 3616 3983 4368 4385 5837 5923
Size: 404 759 481 367 385 17 1452 86
Cuts at: 5923 5935 6281 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 759 481 404 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2865 2865
Size: 6286
BtgI C'CryG_G
Cuts at: 513 1065 3154 513
Size: 552 2089 3645
Fragments arranged by size:
3645 2089 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 2839 3802 4596 4616 2839
Size: 963 794 20 4509
Fragments arranged by size:
4509 963 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6251
DraI TTT'AAA
Cuts at: 2487 3590 2487
Size: 1103 5183
DraIII CAC_nnn'GTG
Cuts at: 2365 2365
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2081 2337 3733
Size: 54 1425 50 275 18 251 256 1396
Cuts at: 3733 4622 6251 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1396 889 275 256 251 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4216 1353
Size: 2863 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2131 2892 2131
Size: 761 5525
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2018 2511 4349 4946 5733 6001 1239
Size: 393 386 493 1838 597 787 268 1524
Fragments arranged by size:
1838 1524 787 597 493 393 386 268
EcoRI G'AATT_C
Cuts at: 2496 3339 3595 3973 2496
Size: 843 256 378 4809
Fragments arranged by size:
4809 843 378 256
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 3282 5433 1678
Size: 1604 2151 2531
Fragments arranged by size:
2531 2151 1604
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2311 2343 2600 2632 2919
Size: 32 135 32 739 32 257 32 287
Cuts at: 2919 2951 4927 4959 5001 5033 1373
Size: 32 1976 32 42 32 2626
Fragments arranged by size:
2626 1976 739 287 257 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2712 3105 748
Size: 1964 393 3929
Fragments arranged by size:
3929 1964 393
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 2564 3611 2564
Size: 1047 5239
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2809 5400 5584 1305
Size: 1504 2591 184 2007
Fragments arranged by size:
2591 2007 1504 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2083 2339 10
Size: 54 1425 343 251 256 3957
Fragments arranged by size:
3957 1425 343 256 251 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4244 4603 4762 518
Size: 648 3078 359 159 2042
Fragments arranged by size:
3078 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2407 3233 3702 4379 5527 5772 1606
Size: 801 826 469 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 801 677 469 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5734
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2671 3644 5189 1132
Size: 97 1442 973 1545 2229
Fragments arranged by size:
2229 1545 1442 973 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6286
PciI A'CATG_T
Cuts at: 2667 5185 2667
Size: 2518 3768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3469 1108
Size: 2361 3925
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 5868 5900 4355
Size: 32 1481 32 4741
Fragments arranged by size:
4741 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3312 3856 3312
Size: 544 5742
PstI C_TGCA'G
Cuts at: 830 3638 830
Size: 2808 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 2407 3233 2407
Size: 826 5460
SacI G_AGCT'C
Cuts at: 2133 2894 2133
Size: 761 5525
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SexAI A'CCwGG_T
Cuts at: 3449 3449
Size: 6286
SfcI C'TryA_G
Cuts at: 826 1045 2657 2704 3634 4769 5450 5641 826
Size: 219 1612 47 930 1135 681 191 1471
Fragments arranged by size:
1612 1471 1135 930 681 219 191 47
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2515 4390 5291 5553 820
Size: 197 926 572 1875 901 262 1553
Fragments arranged by size:
1875 1553 926 901 572 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 3725 513
Size: 552 2660 3074
Fragments arranged by size:
3074 2660 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4313 4330 4483 4668 5113 325
Size: 1255 2733 17 153 185 445 1498
Fragments arranged by size:
2733 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2508 4532 6244
Size: 274 80 33 51 1443 531 2024 1712
Cuts at: 6244 96
Size: 138
Fragments arranged by size:
2024 1712 1443 531 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3219 3251 6246 6278 3219
Size: 32 2995 32 3227
Fragments arranged by size:
3227 2995 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 4415 4415
Size: 6286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI