Restriction map for
pFN21AE6448
(Circular) (Six-base) MAPSORT of: pFN21AE6448.seq Check: 4914 from: 1 to: 6328
pFN21AE6448 6328 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3440 3440
Size: 6328
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2088 2519 2820 4391 5775 6043 1632
Size: 456 431 301 1571 1384 268 1917
Fragments arranged by size:
1917 1571 1384 456 431 301 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 2266 5057 2266
Size: 2791 3537
AleI CACnn'nnGTG
Cuts at: 1166 2474 3153 3321 1166
Size: 1308 679 168 4173
Fragments arranged by size:
4173 1308 679 168
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6328
AlwNI CAG_nnn'CTG
Cuts at: 2134 4071 5643 2134
Size: 1937 1572 2819
Fragments arranged by size:
2819 1937 1572
ApaI G_GGCC'C
Cuts at: 2098 2430 2697 3060 3068 3538 3578 2098
Size: 332 267 363 8 470 40 4848
Fragments arranged by size:
4848 470 363 332 267 40 8
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 3637 3863 4015 6124 1493
Size: 2144 226 152 2109 1697
Fragments arranged by size:
2144 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2488 3570 3653 1870
Size: 73 147 398 1082 83 4545
Fragments arranged by size:
4545 1082 398 147 83 73
BamHI G'GATC_C
Cuts at: 3658 4025 3658
Size: 367 5961
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2785 2849 3044 3219 3649
Size: 325 217 627 998 64 195 175 430
Cuts at: 3649 5104 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 998 627 430 325 217 195
175 64
BanII G_rGCy'C
Cuts at: 1391 2098 2430 2697 3060 3068 3538 3569 3578
Size: 707 332 267 363 8 470 31 9
Cuts at: 3578 3647 1391
Size: 69 4072
Fragments arranged by size:
4072 707 470 363 332 267 69 31
9 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2076 3149 928
Size: 971 177 1073 4107
Fragments arranged by size:
4107 1073 971 177
BbvCI CC'TCA_GC
Cuts at: 2563 2563
Size: 6328
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4519 4553 1903
Size: 34 50 34 2498 34 3678
Fragments arranged by size:
3678 2498 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2056 4220 5430 2056
Size: 2164 1210 2954
Fragments arranged by size:
2954 2164 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2443 4939 136
Size: 107 122 71 631 605 771 2496 1525
Fragments arranged by size:
2496 1525 771 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 2255 3745 2255
Size: 1490 4838
Bme1580I G_kGCm'C
Cuts at: 1790 2098 2287 2430 2697 2790 3060 3068 3260
Size: 308 189 143 267 93 270 8 192
Cuts at: 3260 3538 3578 4389 5545 1790
Size: 278 40 811 1156 2573
Fragments arranged by size:
2573 1156 811 308 278 270 267 192
189 143 93 40 8
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2754 3063 3463
Size: 416 53 83 399 24 1337 309 400
Cuts at: 3463 4059 5017 442
Size: 596 958 1753
Fragments arranged by size:
1753 1337 958 596 416 400 399 309
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2224 2224
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2993 3057 4988 1239
Size: 917 837 64 1931 2579
Fragments arranged by size:
2579 1931 917 837 64
Bpu10I CC'TnA_GC
Cuts at: 2563 2563
Size: 6328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3281 4453 5318 5616 3281
Size: 1172 865 298 3993
Fragments arranged by size:
3993 1172 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3518 4991 882
Size: 617 2019 1473 2219
Fragments arranged by size:
2219 2019 1473 617
BsaAI yAC'GTr
Cuts at: 493 1204 4119 493
Size: 711 2915 2702
Fragments arranged by size:
2915 2702 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4761 5159 5433 5580 5991 4761
Size: 398 274 147 411 5098
Fragments arranged by size:
5098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6103
Fragments arranged by size:
6103 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2174 2324 2694 2990 3042 1186
Size: 973 15 150 370 296 52 4472
Fragments arranged by size:
4472 973 370 296 150 52 15
BseYI C'CCAG_C
Cuts at: 2356 2568 2995 3531 5531 6027 2356
Size: 212 427 536 2000 496 2657
Fragments arranged by size:
2657 2000 536 496 427 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2452 1163
Size: 573 716 5039
Fragments arranged by size:
5039 716 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2281 3778 4539 5567 1540
Size: 275 186 280 1497 761 1028 2301
Fragments arranged by size:
2301 1497 1028 761 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 3569 4389 4474 5545 3569
Size: 820 85 1071 4352
Fragments arranged by size:
4352 1071 820 85
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2098 2287 2430 2697 2790 3060 3068
Size: 399 308 189 143 267 93 270 8
Cuts at: 3068 3260 3538 3569 3578 3647 4389 4474 5545
Size: 192 278 31 9 69 742 85 1071
Cuts at: 5545 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 308 278 270 267
192 189 143 93 85 69 31 9
8
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 3440 3683 844
Size: 2113 483 243 3489
Fragments arranged by size:
3489 2113 483 243
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4972 5159 1700
Size: 110 3162 187 2869
Fragments arranged by size:
3162 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 4388 5400 4388
Size: 1012 5316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2291 794
Size: 1497 4831
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstXI CCAn_nnnn'nTGG
Cuts at: 2372 3127 2372
Size: 755 5573
BstYI r'GATC_y
Cuts at: 1972 2131 3658 4025 4410 4427 5879 5965 5977
Size: 159 1527 367 385 17 1452 86 12
Cuts at: 5977 6323 1972
Size: 346 1977
Fragments arranged by size:
1977 1527 1452 385 367 346 159 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2666 2666
Size: 6328
BtgI C'CryG_G
Cuts at: 513 1065 2441 3444 3490 513
Size: 552 1376 1003 46 3351
Fragments arranged by size:
3351 1376 1003 552 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2632 2921 3844 4638 4658 2632
Size: 289 923 794 20 4302
Fragments arranged by size:
4302 923 794 289 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DraIII CAC_nnn'GTG
Cuts at: 2291 2846 3152 2291
Size: 555 306 5467
Fragments arranged by size:
5467 555 306
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2278 2545 3288
Size: 54 1425 50 275 18 448 267 743
Cuts at: 3288 3487 3775 4664 6293 8
Size: 199 288 889 1629 43
Fragments arranged by size:
1629 1425 889 743 448 288 275 267
199 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2278 3775 1537
Size: 275 466 1497 4090
Fragments arranged by size:
4090 1497 466 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2151 4258 1353
Size: 792 6 2107 3423
Fragments arranged by size:
3423 2107 792 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3567 3567
Size: 6328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2088 2156 2519 2820 2993 3057 4391
Size: 393 456 68 363 301 173 64 1334
Cuts at: 4391 4988 5775 6043 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1334 787 597 456 393 363 301
268 173 68 64
EcoO109I rG'GnC_Cy
Cuts at: 2087 2094 2095 2426 2427 2693 2694 2896 3056
Size: 7 1 331 1 266 1 202 160
Cuts at: 3056 3057 3064 3065 3506 3535 3574 2087
Size: 1 7 1 441 29 39 4841
Fragments arranged by size:
4841 441 331 266 202 160 39 29
7 7 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 3637 4015 3637
Size: 378 5950
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 3019 3472 5475 1678
Size: 1341 453 2003 2531
Fragments arranged by size:
2531 2003 1341 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2252 2284 2659 2691 4969
Size: 32 135 32 680 32 375 32 2278
Cuts at: 4969 5001 5043 5075 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2278 680 375 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2578 3672 3918 669
Size: 988 281 640 1094 246 3079
Fragments arranged by size:
3079 1094 988 640 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5442 5626 1305
Size: 4137 184 2007
Fragments arranged by size:
4137 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3290 3489 10
Size: 54 1425 343 1458 199 2849
Fragments arranged by size:
2849 1458 1425 343 199 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3153 3321 4286 4645 4804 518
Size: 648 1308 679 168 965 359 159 2042
Fragments arranged by size:
2042 1308 965 679 648 359 168 159
MspA1I CmG'CkG
Cuts at: 1606 2368 2523 2541 2550 2625 2730 2878 3171
Size: 762 155 18 9 75 105 148 293
Cuts at: 3171 3180 3360 3423 3744 4421 5569 5814 1606
Size: 9 180 63 321 677 1148 245 2120
Fragments arranged by size:
2120 1148 762 677 321 293 245 180
155 148 105 75 63 18 9 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5776
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 2512 3025 3394 3686 5231 1132
Size: 97 1283 513 369 292 1545 2229
Fragments arranged by size:
2229 1545 1283 513 369 292 97
PasI CC'CwG_GG
Cuts at: 1348 3061 3586 1348
Size: 1713 525 4090
Fragments arranged by size:
4090 1713 525
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2134 2191 2846 2870 1108
Size: 1026 57 655 24 4566
Fragments arranged by size:
4566 1026 655 57 24
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2519 2551 4397 4429 5910 5942 2519
Size: 32 1846 32 1481 32 2905
Fragments arranged by size:
2905 1846 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2087 2087
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PspOMI G'GGCC_C
Cuts at: 2094 2426 2693 3056 3064 3534 3574 2094
Size: 332 267 363 8 470 40 4848
Fragments arranged by size:
4848 470 363 332 267 40 8
PspXI vC'TCGA_Gb
Cuts at: 2488 2488
Size: 6328
PstI C_TGCA'G
Cuts at: 830 2651 3680 830
Size: 1821 1029 3478
Fragments arranged by size:
3478 1821 1029
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
PvuII CAG'CTG
Cuts at: 2368 2878 3360 2368
Size: 510 482 5336
Fragments arranged by size:
5336 510 482
SacI G_AGCT'C
Cuts at: 3569 3569
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SexAI A'CCwGG_T
Cuts at: 2842 2842
Size: 6328
SfcI C'TryA_G
Cuts at: 826 1045 2647 3088 3355 3676 4811 5492 5683
Size: 219 1602 441 267 321 1135 681 191
Cuts at: 5683 826
Size: 1471
Fragments arranged by size:
1602 1471 1135 681 441 321 267 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 2092 3655 1872
Size: 220 1563 4545
Fragments arranged by size:
4545 1563 220
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2488 3296 4432 5333 5595 820
Size: 197 926 545 808 1136 901 262 1553
Fragments arranged by size:
1553 1136 926 901 808 545 262 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3025 3686 1132
Size: 1893 661 3774
Fragments arranged by size:
3774 1893 661
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 2236 1517
Size: 719 5609
StyI C'CwwG_G
Cuts at: 513 1065 2942 3767 513
Size: 552 1877 825 3074
Fragments arranged by size:
3074 1877 825 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2615 4355 4372 4525 4710 5155 325
Size: 1255 1035 1740 17 153 185 445 1498
Fragments arranged by size:
1740 1498 1255 1035 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 2735 4574
Size: 274 80 33 51 1443 474 284 1839
Cuts at: 4574 6286 96
Size: 1712 138
Fragments arranged by size:
1839 1712 1443 474 284 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3100 3132 6288 6320 3100
Size: 32 3156 32 3108
Fragments arranged by size:
3156 3108 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3482 3482
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 2488 1943
Size: 545 5783
XmaI C'CCGG_G
Cuts at: 1870 2090 3653 1870
Size: 220 1563 4545
Fragments arranged by size:
4545 1563 220
XmnI GAAnn'nnTTC
Cuts at: 2534 3164 3416 4457 2534
Size: 630 252 1041 4405
Fragments arranged by size:
4405 1041 630 252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI