Restriction map for
pFN21AE6446
(Circular) (Six-base) MAPSORT of: pFN21AE6446.seq Check: 3510 from: 1 to: 6220
pFN21AE6446 6220 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2644 2644
Size: 6220
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4030 278
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3563 1937
Size: 1626 4594
Acc65I G'GTAC_C
Cuts at: 2513 3541 2513
Size: 1028 5192
AclI AA'CG_TT
Cuts at: 1563 4347 4720 1563
Size: 2784 373 3063
Fragments arranged by size:
3063 2784 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2347 2833 4283 5667 5935 1632
Size: 715 486 1450 1384 268 1917
Fragments arranged by size:
1917 1450 1384 715 486 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6023
AflIII A'CryG_T
Cuts at: 1203 2099 3201 5119 6096 1203
Size: 896 1102 1918 977 1327
Fragments arranged by size:
1918 1327 1102 977 896
AgeI A'CCGG_T
Cuts at: 5051 5051
Size: 6220
AhdI GACnn_n'nnGTC
Cuts at: 4949 4949
Size: 6220
AleI CACnn'nnGTG
Cuts at: 1166 2066 2186 2588 1166
Size: 900 120 402 4798
Fragments arranged by size:
4798 900 402 120
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 2662 1184
Size: 1218 260 4742
Fragments arranged by size:
4742 1218 260
AlwNI CAG_nnn'CTG
Cuts at: 2017 3963 5535 2017
Size: 1946 1572 2702
Fragments arranged by size:
2702 1946 1572
ApaI G_GGCC'C
Cuts at: 2208 2208
Size: 6220
ApaLI G'TGCA_C
Cuts at: 4277 5433 4277
Size: 1156 5064
ApoI r'AATT_y
Cuts at: 1493 3529 3755 3907 6016 1493
Size: 2036 226 152 2109 1697
Fragments arranged by size:
2109 2036 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4774 160
Size: 872 3742 1606
Fragments arranged by size:
3742 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 3545 1870
Size: 73 684 918 4545
Fragments arranged by size:
4545 918 684 73
BamHI G'GATC_C
Cuts at: 3550 3917 3550
Size: 367 5853
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2513 2782 3041 3541 4996
Size: 325 217 627 726 269 259 500 1455
Cuts at: 4996 618
Size: 1842
Fragments arranged by size:
1842 1455 726 627 500 325 269 259
217
BanII G_rGCy'C
Cuts at: 1391 2208 2955 3100 3539 1391
Size: 817 747 145 439 4072
Fragments arranged by size:
4072 817 747 439 145
BbeI G_GCGC'C
Cuts at: 3045 3045
Size: 6220
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2518 2767 3369 928
Size: 971 619 249 602 3779
Fragments arranged by size:
3779 971 619 602 249
BbvCI CC'TCA_GC
Cuts at: 2312 2312
Size: 6220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2618 2652 4411 4445 1903
Size: 34 50 34 597 34 1759 34 3678
Fragments arranged by size:
3678 1759 597 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4112 5322 4112
Size: 1210 5010
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6220
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4831 136
Size: 107 122 71 631 605 3159 1525
Fragments arranged by size:
3159 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6215 6215
Size: 6220
BlpI GC'TnA_GC
Cuts at: 3637 3637
Size: 6220
Bme1580I G_kGCm'C
Cuts at: 1790 2208 4281 5437 1790
Size: 418 2073 1156 2573
Fragments arranged by size:
2573 2073 1156 418
BmgBI CAC'GTC
Cuts at: 1318 3392 1318
Size: 2074 4146
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2741 3951 4909
Size: 416 53 83 399 24 1324 1210 958
Cuts at: 4909 442
Size: 1753
Fragments arranged by size:
1753 1324 1210 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2910 1056
Size: 1854 4366
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2039 2513 2527 3326 3421 4880 1239
Size: 800 474 14 799 95 1459 2579
Fragments arranged by size:
2579 1459 800 799 474 95 14
Bpu10I CC'TnA_GC
Cuts at: 2312 2312
Size: 6220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4345 5210 5508 4345
Size: 865 298 5057
Fragments arranged by size:
5057 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 2594 4883 882
Size: 617 651 444 2289 2219
Fragments arranged by size:
2289 2219 651 617 444
BsaAI yAC'GTr
Cuts at: 493 1204 2100 4011 493
Size: 711 896 1911 2702
Fragments arranged by size:
2702 1911 896 711
BsaBI GATnn'nnATC
Cuts at: 6214 6214
Size: 6220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2445 3042 3171 3402
Size: 53 83 186 936 912 597 129 231
Cuts at: 3402 4027 4409 5044 275
Size: 625 382 635 1451
Fragments arranged by size:
1451 936 912 635 625 597 382 231
186 129 83 53
BsaWI w'CCGG_w
Cuts at: 4653 5051 5325 5472 5883 4653
Size: 398 274 147 411 4990
Fragments arranged by size:
4990 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5995
Fragments arranged by size:
5995 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2819 3424 1186
Size: 1633 605 3982
Fragments arranged by size:
3982 1633 605
BseYI C'CCAG_C
Cuts at: 2037 2257 2389 5423 5919 2037
Size: 220 132 3034 496 2338
Fragments arranged by size:
3034 2338 496 220 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2046 2233 2622 1163
Size: 573 310 187 389 4761
Fragments arranged by size:
4761 573 389 310 187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2665 2897 3670 4431 5459 1540
Size: 275 186 664 232 773 761 1028 2301
Fragments arranged by size:
2301 1028 773 761 664 275 232 186
BsiHKAI G_wGCw'C
Cuts at: 2193 2997 4281 4366 5437 2193
Size: 804 1284 85 1071 2976
Fragments arranged by size:
2976 1284 1071 804 85
BsmI GAATG_Cn'
Cuts at: 3731 3824 3731
Size: 93 6127
BsmBI CGTCTCn'nnnn_
Cuts at: 2452 2452
Size: 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2208 2955 2997 3100 3539 4281
Size: 399 403 15 747 42 103 439 742
Cuts at: 4281 4366 5437 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 439 403 399 103
85 42 15
BspEI T'CCGG_A
Cuts at: 5883 5883
Size: 6220
BspHI T'CATG_A
Cuts at: 4109 5839 4109
Size: 1730 4490
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 3575 844
Size: 1800 931 3489
Fragments arranged by size:
3489 1800 931
BsrBI CCG'CTC
Cuts at: 1394 1823 3310 4107 1394
Size: 429 1487 797 3507
Fragments arranged by size:
3507 1487 797 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2135 4715 4889 65
Size: 1183 887 2580 174 1396
Fragments arranged by size:
2580 1396 1183 887 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2409 2896 4864 5051 1700
Size: 110 599 487 1968 187 2869
Fragments arranged by size:
2869 1968 599 487 187 110
BsrGI T'GTAC_A
Cuts at: 96 2724 96
Size: 2628 3592
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6220
BssSI C'ACGA_G
Cuts at: 2996 4280 5292 2996
Size: 1284 1012 3924
Fragments arranged by size:
3924 1284 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6220
BstBI TT'CG_AA
Cuts at: 5024 5024
Size: 6220
BstEII G'GTnAC_C
Cuts at: 1184 2068 1184
Size: 884 5336
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2590 3100 2431
Size: 159 510 5551
Fragments arranged by size:
5551 510 159
BstYI r'GATC_y
Cuts at: 1972 2535 3313 3550 3917 4302 4319 5771 5857
Size: 563 778 237 367 385 17 1452 86
Cuts at: 5857 5869 6215 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 778 563 385 367 346 237
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2864 2864
Size: 6220
BtgI C'CryG_G
Cuts at: 513 1065 2064 3078 513
Size: 552 999 1014 3655
Fragments arranged by size:
3655 1014 999 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5481
Fragments arranged by size:
5481 683 56
BtsI GCAGTG_nn'
Cuts at: 2545 2762 3736 4530 4550 2545
Size: 217 974 794 20 4215
Fragments arranged by size:
4215 974 794 217 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6185
DraI TTT'AAA
Cuts at: 3524 3524
Size: 6220
DraIII CAC_nnn'GTG
Cuts at: 2806 2806
Size: 6220
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5227 809
Size: 507 3911 1802
Fragments arranged by size:
3911 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 2271 2289
Size: 54 1425 50 275 18 228 213 18
Cuts at: 2289 2421 2468 2580 2662 2894 3153 3464 3667
Size: 132 47 112 82 232 259 311 203
Cuts at: 3667 4556 6185 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 311 275 259 232 228
213 203 132 112 82 54 50 47
43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2662 2894 3667 1537
Size: 275 850 232 773 4090
Fragments arranged by size:
4090 850 773 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 3113 3239 4150 1353
Size: 1760 126 911 3423
Fragments arranged by size:
3423 1760 911 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2396 3311 4807 5181 5327 387
Size: 1064 138 807 915 1496 374 146 1280
Fragments arranged by size:
1496 1280 1064 915 807 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2347 2513 2527 2833 3326 3421
Size: 393 407 308 166 14 306 493 95
Cuts at: 3421 4283 4880 5667 5935 1239
Size: 862 597 787 268 1524
Fragments arranged by size:
1524 862 787 597 493 407 393 308
306 268 166 95 14
EcoNI CCTnn'n_nnAGG
Cuts at: 3436 3436
Size: 6220
EcoO109I rG'GnC_Cy
Cuts at: 2014 2077 2204 2205 2868 2014
Size: 63 127 1 663 5366
Fragments arranged by size:
5366 663 127 63 1
EcoRI G'AATT_C
Cuts at: 3529 3907 3529
Size: 378 5842
FseI GG_CCGG'CC
Cuts at: 2900 2900
Size: 6220
FspI TGC'GCA
Cuts at: 4726 4726
Size: 6220
HaeII r_GCGC'y
Cuts at: 1678 2680 3045 5367 1678
Size: 1002 365 2322 2531
Fragments arranged by size:
2531 2322 1002 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4861 4893 4935 4967 1373
Size: 32 135 32 3289 32 42 32 2626
Fragments arranged by size:
3289 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3564 3810 669
Size: 988 281 1626 246 3079
Fragments arranged by size:
3079 1626 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6220
HpaI GTT'AAC
Cuts at: 3810 3810
Size: 6220
KasI G'GCGC_C
Cuts at: 3041 3041
Size: 6220
KpnI G_GTAC'C
Cuts at: 2517 3545 2517
Size: 1028 5192
MfeI C'AATT_G
Cuts at: 3819 3819
Size: 6220
MluI A'CGCG_T
Cuts at: 6096 6096
Size: 6220
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5334 5518 1305
Size: 4029 184 2007
Fragments arranged by size:
4029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2060 2273 2291 2423 2582
Size: 54 1425 343 228 213 18 132 159
Cuts at: 2582 3466 10
Size: 884 2764
Fragments arranged by size:
2764 1425 884 343 228 213 159 132
54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2186 2222 2384 2588 4178 4537
Size: 648 900 120 36 162 204 1590 359
Cuts at: 4537 4696 518
Size: 159 2042
Fragments arranged by size:
2042 1590 900 648 359 204 162 159
120 36
MspA1I CmG'CkG
Cuts at: 1606 2037 2473 3227 3398 3636 4313 5461 5706
Size: 431 436 754 171 238 677 1148 245
Cuts at: 5706 1606
Size: 2120
Fragments arranged by size:
2120 1148 754 677 436 431 245 238
171
NaeI GCC'GGC
Cuts at: 2411 2898 2411
Size: 487 5733
NarI GG'CG_CC
Cuts at: 3042 3042
Size: 6220
NcoI C'CATG_G
Cuts at: 513 1065 2064 3078 513
Size: 552 999 1014 3655
Fragments arranged by size:
3655 1014 999 552
NdeI CA'TA_TG
Cuts at: 387 2788 387
Size: 2401 3819
NgoMIV G'CCGG_C
Cuts at: 2409 2896 2409
Size: 487 5733
NheI G'CTAG_C
Cuts at: 1052 2906 1052
Size: 1854 4366
NotI GC'GGCC_GC
Cuts at: 3667 3667
Size: 6220
NspI r_CATG'y
Cuts at: 1132 1229 2359 2661 3205 3578 5123 1132
Size: 97 1130 302 544 373 1545 2229
Fragments arranged by size:
2229 1545 1130 544 373 302 97
PasI CC'CwG_GG
Cuts at: 1348 2074 2086 2452 3100 1348
Size: 726 12 366 648 4468
Fragments arranged by size:
4468 726 648 366 12
PciI A'CATG_T
Cuts at: 3201 5119 3201
Size: 1918 4302
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2017 1108
Size: 909 5311
PfoI T'CCnGG_A
Cuts at: 3117 3165 3437 3117
Size: 48 272 5900
Fragments arranged by size:
5900 272 48
PmeI GTTT'AAAC
Cuts at: 3524 3524
Size: 6220
PmlI CAC'GTG
Cuts at: 2100 2100
Size: 6220
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4289 4321 5802 5834 4289
Size: 32 1481 32 4675
Fragments arranged by size:
4675 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6220
PsiI TTA'TAA
Cuts at: 3790 3790
Size: 6220
PspOMI G'GGCC_C
Cuts at: 2204 2204
Size: 6220
PstI C_TGCA'G
Cuts at: 830 3264 3483 3572 830
Size: 2434 219 89 3478
Fragments arranged by size:
3478 2434 219 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6220
PvuII CAG'CTG
Cuts at: 2037 3227 3398 2037
Size: 1190 171 4859
Fragments arranged by size:
4859 1190 171
SalI G'TCGA_C
Cuts at: 1936 3562 1936
Size: 1626 4594
SbfI CC_TGCA'GG
Cuts at: 3572 3572
Size: 6220
ScaI AGT'ACT
Cuts at: 4468 6180 4468
Size: 1712 4508
SexAI A'CCwGG_T
Cuts at: 2961 2961
Size: 6220
SfcI C'TryA_G
Cuts at: 826 1045 3260 3479 3568 4703 5384 5575 826
Size: 219 2215 219 89 1135 681 191 1471
Fragments arranged by size:
2215 1471 1135 681 219 219 191 89
SfoI GGC'GCC
Cuts at: 3043 3043
Size: 6220
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6220
SgrAI Cr'CCGG_yG
Cuts at: 1700 2409 1700
Size: 709 5511
SmaI CCC'GGG
Cuts at: 1872 3547 1872
Size: 1675 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4324 5225 5487 820
Size: 197 926 2381 901 262 1553
Fragments arranged by size:
2381 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4011 493
Size: 3518 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6220
SphI G_CATG'C
Cuts at: 1132 2661 3578 1132
Size: 1529 917 3774
Fragments arranged by size:
3774 1529 917
SspI AAT'ATT
Cuts at: 5 52 4144 5
Size: 47 4092 2081
Fragments arranged by size:
4092 2081 47
StuI AGG'CCT
Cuts at: 1517 2349 2575 1517
Size: 832 226 5162
Fragments arranged by size:
5162 832 226
StyI C'CwwG_G
Cuts at: 513 1065 2064 3078 3659 513
Size: 552 999 1014 581 3074
Fragments arranged by size:
3074 1014 999 581 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 2723 2980 3230 4247 4264 4417
Size: 1255 455 688 257 250 1017 17 153
Cuts at: 4417 4602 5047 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1017 688 455 445 257 250
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 4466 6178
Size: 274 80 33 51 1443 747 1742 1712
Cuts at: 6178 96
Size: 138
Fragments arranged by size:
1742 1712 1443 747 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2760 2792 6180 6212 2760
Size: 32 3388 32 2768
Fragments arranged by size:
3388 2768 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3071 1316
Size: 1755 4465
XbaI T'CTAG_A
Cuts at: 3556 3556
Size: 6220
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2281 2793 2281
Size: 512 5708
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6220
XmaI C'CCGG_G
Cuts at: 1870 3545 1870
Size: 1675 4545
XmnI GAAnn'nnTTC
Cuts at: 4349 4349
Size: 6220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4028 276
Size: 53 83 186 936 2494 2468
Fragments arranged by size:
2494 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoICRI EcoRV FalI FspAI NruI NsiI PacI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SrfI SwaI