Restriction map for
pFN21AE6440
(Circular) (Six-base) MAPSORT of: pFN21AE6440.seq Check: 2286 from: 1 to: 6430
pFN21AE6440 6430 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2258 3231 2258
Size: 973 5457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2045 3065 3224 3408 4493 5877 6145 1632
Size: 413 1020 159 184 1085 1384 268 1917
Fragments arranged by size:
1917 1384 1085 1020 413 268 184 159
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 2432 5159 2432
Size: 2727 3703
AleI CACnn'nnGTG
Cuts at: 1166 2124 1166
Size: 958 5472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2237 1184
Size: 1053 5377
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3668 3700 3668
Size: 32 6398
AlwNI CAG_nnn'CTG
Cuts at: 3202 3378 4173 5745 3202
Size: 176 795 1572 3887
Fragments arranged by size:
3887 1572 795 176
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 2405 2744 3050 3214 3479 3739 3965 4117
Size: 912 339 306 164 265 260 226 152
Cuts at: 4117 6226 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 912 339 306 265 260 226
164 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 3755 1870
Size: 73 1812 4545
Fragments arranged by size:
4545 1812 73
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 3751 5206 618
Size: 325 217 627 248 1716 1455 1842
Fragments arranged by size:
1842 1716 1455 627 325 248 217
BanII G_rGCy'C
Cuts at: 1391 2803 3579 3749 1391
Size: 1412 776 170 4072
Fragments arranged by size:
4072 1412 776 170
BbeI G_GCGC'C
Cuts at: 2039 2039
Size: 6430
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3387 928
Size: 971 1488 3971
Fragments arranged by size:
3971 1488 971
BbvCI CC'TCA_GC
Cuts at: 2068 2068
Size: 6430
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4621 4655 1903
Size: 34 50 34 2600 34 3678
Fragments arranged by size:
3678 2600 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5041 136
Size: 107 122 71 631 605 3369 1525
Fragments arranged by size:
3369 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2505 6425 2505
Size: 3920 2510
BlpI GC'TnA_GC
Cuts at: 2297 3847 2297
Size: 1550 4880
Bme1580I G_kGCm'C
Cuts at: 1790 2121 3729 4491 5647 1790
Size: 331 1608 762 1156 2573
Fragments arranged by size:
2573 1608 1156 762 331
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2677 3143 4161
Size: 416 53 83 399 24 1260 466 1018
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1753 1260 1018 958 466 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2061 2357 3350 3635 5090 1239
Size: 822 296 993 285 1455 2579
Fragments arranged by size:
2579 1455 993 822 296 285
Bpu10I CC'TnA_GC
Cuts at: 2068 2068
Size: 6430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2154 4555 5420 5718 2154
Size: 2401 865 298 2866
Fragments arranged by size:
2866 2401 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3708 5093 882
Size: 617 2209 1385 2219
Fragments arranged by size:
2219 2209 1385 617
BsaAI yAC'GTr
Cuts at: 493 1204 4221 493
Size: 711 3017 2702
Fragments arranged by size:
3017 2702 711
BsaBI GATnn'nnATC
Cuts at: 2676 6424 2676
Size: 3748 2682
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 4237 4619 5254
Size: 53 83 186 936 503 2201 382 635
Cuts at: 5254 275
Size: 1451
Fragments arranged by size:
2201 1451 936 635 503 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2195 2225 2605 2635 3668
Size: 30 165 30 792 30 380 30 1033
Cuts at: 3668 3698 1178
Size: 30 3910
Fragments arranged by size:
3910 1033 792 380 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6430
BseYI C'CCAG_C
Cuts at: 2102 2782 2795 3154 5633 6129 2102
Size: 680 13 359 2479 496 2403
Fragments arranged by size:
2479 2403 680 496 359 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2233 3665 1163
Size: 573 497 1432 3928
Fragments arranged by size:
3928 1432 573 497
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3880 4641 5669 1540
Size: 275 186 1879 761 1028 2301
Fragments arranged by size:
2301 1879 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4491 4576 5647 4491
Size: 85 1071 5274
Fragments arranged by size:
5274 1071 85
BsmI GAATG_Cn'
Cuts at: 2317 3941 4034 2317
Size: 1624 93 4713
Fragments arranged by size:
4713 1624 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 2803 3579 3729 3749 4491 4576
Size: 399 331 682 776 150 20 742 85
Cuts at: 4576 5647 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 776 742 682 399 331 150
85 20
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2229 2258 3231 3785 844
Size: 1385 29 973 554 3489
Fragments arranged by size:
3489 1385 973 554 29
BsrBI CCG'CTC
Cuts at: 1394 1823 4317 1394
Size: 429 2494 3507
Fragments arranged by size:
3507 2494 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5074 5261 1700
Size: 110 3264 187 2869
Fragments arranged by size:
3264 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstXI CCAn_nnnn'nTGG
Cuts at: 2909 3604 2909
Size: 695 5735
BstYI r'GATC_y
Cuts at: 1972 2505 3760 4127 4512 4529 5981 6067 6079
Size: 533 1255 367 385 17 1452 86 12
Cuts at: 6079 6425 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1255 533 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3382 3579 513
Size: 552 2317 197 3364
Fragments arranged by size:
3364 2317 552 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 2588 3139 3946 4740 4760 2588
Size: 551 807 794 20 4258
Fragments arranged by size:
4258 807 794 551 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2359 2499 3310
Size: 54 1425 50 275 18 529 140 811
Cuts at: 3310 3877 4766 6395 8
Size: 567 889 1629 43
Fragments arranged by size:
1629 1425 889 811 567 529 275 140
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3877 1537
Size: 275 2065 4090
Fragments arranged by size:
4090 2065 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2064 2391 3134 3539 4360 1353
Size: 711 327 743 405 821 3423
Fragments arranged by size:
3423 821 743 711 405 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3567 3638 5017 5391 5537 387
Size: 1064 138 1978 71 1379 374 146 1280
Fragments arranged by size:
1978 1379 1280 1064 374 146 138 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2061 2357 3065 3224 3350 3408
Size: 393 413 16 296 708 159 126 58
Cuts at: 3408 3635 4493 5090 5877 6145 1239
Size: 227 858 597 787 268 1524
Fragments arranged by size:
1524 858 787 708 597 413 393 296
268 227 159 126 58 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3671 3671
Size: 6430
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
EcoRV GAT'ATC
Cuts at: 3299 3299
Size: 6430
FspI TGC'GCA
Cuts at: 3339 4936 3339
Size: 1597 4833
HaeII r_GCGC'y
Cuts at: 1678 2039 5577 1678
Size: 361 3538 2531
Fragments arranged by size:
3538 2531 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2268 2300 2418
Size: 32 135 32 506 32 158 32 118
Cuts at: 2418 2450 5071 5103 5145 5177 1373
Size: 32 2621 32 42 32 2626
Fragments arranged by size:
2626 2621 506 158 135 118 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3061 748
Size: 2313 4117
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 2035 2035
Size: 6430
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2920 3063 3210 3423 3471 5544 5728 1305
Size: 1615 143 147 213 48 2073 184 2007
Fragments arranged by size:
2073 2007 1615 213 184 147 143 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2361 2501 10
Size: 54 1425 343 529 140 3939
Fragments arranged by size:
3939 1425 529 343 140 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2124 4388 4747 4906 518
Size: 648 958 2264 359 159 2042
Fragments arranged by size:
2264 2042 958 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2795 3846 4523 5671 5916 1606
Size: 1189 1051 677 1148 245 2120
Fragments arranged by size:
2120 1189 1148 1051 677 245
NarI GG'CG_CC
Cuts at: 2036 2036
Size: 6430
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5878
NdeI CA'TA_TG
Cuts at: 387 2734 387
Size: 2347 4083
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3788 5333 1132
Size: 97 2559 1545 2229
Fragments arranged by size:
2559 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2766 1348
Size: 1418 5012
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2723 3667 1108
Size: 1615 944 3871
Fragments arranged by size:
3871 1615 944
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 4499 4531 6012 6044 3668
Size: 32 799 32 1481 32 4054
Fragments arranged by size:
4054 1481 799 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PstI C_TGCA'G
Cuts at: 830 3134 3782 830
Size: 2304 648 3478
Fragments arranged by size:
3478 2304 648
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2795 2795
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 4678 6390 4678
Size: 1712 4718
SfcI C'TryA_G
Cuts at: 826 1045 2414 2916 3130 3778 4913 5594 5785
Size: 219 1369 502 214 648 1135 681 191
Cuts at: 5785 826
Size: 1471
Fragments arranged by size:
1471 1369 1135 681 648 502 219 214
191
SfoI GGC'GCC
Cuts at: 2037 2037
Size: 6430
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 3757 1872
Size: 1885 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2169 4534 5435 5697 820
Size: 197 926 226 2365 901 262 1553
Fragments arranged by size:
2365 1553 926 901 262 226 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 2459 3013 4354 5
Size: 47 2407 554 1341 2081
Fragments arranged by size:
2407 2081 1341 554 47
StuI AGG'CCT
Cuts at: 1517 2195 1517
Size: 678 5752
StyI C'CwwG_G
Cuts at: 513 1065 2391 3718 3869 513
Size: 552 1326 1327 151 3074
Fragments arranged by size:
3074 1327 1326 552 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4457 4474 4627 4812 5257 325
Size: 1255 2877 17 153 185 445 1498
Fragments arranged by size:
2877 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3345 4676 6388
Size: 274 80 33 51 1443 1368 1331 1712
Cuts at: 6388 96
Size: 138
Fragments arranged by size:
1712 1443 1368 1331 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 6390 6422 2195
Size: 32 4163 32 2203
Fragments arranged by size:
4163 2203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3149 1316
Size: 1833 4597
XbaI T'CTAG_A
Cuts at: 2462 3068 3766 2462
Size: 606 698 5126
Fragments arranged by size:
5126 698 606
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3227 3227
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 3755 1870
Size: 1885 4545
XmnI GAAnn'nnTTC
Cuts at: 2470 2744 2855 3683 4559 2470
Size: 274 111 828 876 4341
Fragments arranged by size:
4341 876 828 274 111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoICRI EcoO109I FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI