Restriction map for
pFN21AE6437
(Circular) (Six-base) MAPSORT of: pFN21AE6437.seq Check: 525 from: 1 to: 6379
pFN21AE6437 6379 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2099 2099
Size: 6379
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 2654 3700 2654
Size: 1046 5333
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2661 4442 5826 6094 1632
Size: 1029 1781 1384 268 1917
Fragments arranged by size:
1917 1781 1384 1029 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6182
AflIII A'CryG_T
Cuts at: 1203 2560 5278 6255 1203
Size: 1357 2718 977 1327
Fragments arranged by size:
2718 1357 1327 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 3646 5108 3646
Size: 1462 4917
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6379
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6379
AlwNI CAG_nnn'CTG
Cuts at: 2515 4122 5694 2515
Size: 1607 1572 3200
Fragments arranged by size:
3200 1607 1572
ApaI G_GGCC'C
Cuts at: 3431 3431
Size: 6379
ApaLI G'TGCA_C
Cuts at: 4436 5592 4436
Size: 1156 5223
ApoI r'AATT_y
Cuts at: 1493 2261 2282 2490 2843 2915 3284 3688 3914
Size: 768 21 208 353 72 369 404 226
Cuts at: 3914 4066 6175 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 768 404 369 353 226 208
152 72 21
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 3704 1870
Size: 73 1761 4545
Fragments arranged by size:
4545 1761 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2646 2679 2646
Size: 33 6346
BamHI G'GATC_C
Cuts at: 3128 3364 3709 4076 3128
Size: 236 345 367 5431
Fragments arranged by size:
5431 367 345 236
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2654 3562 3700 5155 618
Size: 325 217 627 867 908 138 1455 1842
Fragments arranged by size:
1842 1455 908 867 627 325 217 138
BanII G_rGCy'C
Cuts at: 1391 2088 3153 3431 3698 1391
Size: 697 1065 278 267 4072
Fragments arranged by size:
4072 1065 697 278 267
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4570 4604 1903
Size: 34 2633 34 3678
Fragments arranged by size:
3678 2633 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2885 4271 5481 2885
Size: 1386 1210 3783
Fragments arranged by size:
3783 1386 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3117 3426 3560
Size: 107 122 71 631 605 1445 309 134
Cuts at: 3560 4990 136
Size: 1430 1525
Fragments arranged by size:
1525 1445 1430 631 605 309 134 122
107 71
BglII A'GATC_T
Cuts at: 6374 6374
Size: 6379
BlpI GC'TnA_GC
Cuts at: 2289 3796 2289
Size: 1507 4872
Bme1580I G_kGCm'C
Cuts at: 1790 3431 3565 4440 5596 1790
Size: 1641 134 875 1156 2573
Fragments arranged by size:
2573 1641 1156 875 134
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3463 4110 5068
Size: 416 53 83 399 24 2046 647 958
Cuts at: 5068 442
Size: 1753
Fragments arranged by size:
2046 1753 958 647 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6379
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2610 5039 1239
Size: 863 508 2429 2579
Fragments arranged by size:
2579 2429 863 508
Bpu10I CC'TnA_GC
Cuts at: 2628 2628
Size: 6379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3344 4504 5369 5667 3344
Size: 1160 865 298 4056
Fragments arranged by size:
4056 1160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5042 882
Size: 617 3543 2219
Fragments arranged by size:
3543 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4812 5210 5484 5631 6042 4812
Size: 398 274 147 411 5149
Fragments arranged by size:
5149 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2022 2052 1178
Size: 30 165 30 619 30 5505
Fragments arranged by size:
5505 619 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2041 2607 3690 1186
Size: 855 566 1083 3875
Fragments arranged by size:
3875 1083 855 566
BseYI C'CCAG_C
Cuts at: 3338 5582 6078 3338
Size: 2244 496 3639
Fragments arranged by size:
3639 2244 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2356 1163
Size: 573 620 5186
Fragments arranged by size:
5186 620 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 3153 4440 4525 5596 2295
Size: 858 1287 85 1071 3078
Fragments arranged by size:
3078 1287 1071 858 85
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2295 3153 3431 3565 3698 4440
Size: 399 298 207 858 278 134 133 742
Cuts at: 4440 4525 5596 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 858 742 399 298 278 207
134 133 85
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 2247 2800 4268 5998 2247
Size: 553 1468 1730 2628
Fragments arranged by size:
2628 1730 1468 553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2099 3734 844
Size: 1255 1635 3489
Fragments arranged by size:
3489 1635 1255
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2598 3294 4874 5048 65
Size: 1183 1350 696 1580 174 1396
Fragments arranged by size:
1580 1396 1350 1183 696 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5023 5210 1700
Size: 110 3213 187 2869
Fragments arranged by size:
3213 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3038 96
Size: 2942 3437
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6379
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2511 2535 3128 3364 3709 4076 4461 4478
Size: 539 24 593 236 345 367 385 17
Cuts at: 4478 5930 6016 6028 6374 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 593 539 385 367 346 345
236 86 24 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2028 3895 4689 4709 2028
Size: 1867 794 20 3698
Fragments arranged by size:
3698 1867 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2077 2112 529
Size: 35 1513 35 4796
Fragments arranged by size:
4796 1513 35 35
DraI TTT'AAA
Cuts at: 2503 3265 3495 3549 3683 2503
Size: 762 230 54 134 5199
Fragments arranged by size:
5199 762 230 134 54
DraIII CAC_nnn'GTG
Cuts at: 2334 2334
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3826 4715
Size: 54 1425 50 275 18 177 1819 889
Cuts at: 4715 6344 8
Size: 1629 43
Fragments arranged by size:
1819 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2680 3588 4309 1353
Size: 1327 908 721 3423
Fragments arranged by size:
3423 1327 908 721
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3151 3151
Size: 6379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2610 2661 4442 5039 5826 6094
Size: 393 470 508 51 1781 597 787 268
Cuts at: 6094 1239
Size: 1524
Fragments arranged by size:
1781 1524 787 597 508 470 393 268
51
EcoO109I rG'GnC_Cy
Cuts at: 2077 3428 2077
Size: 1351 5028
EcoRI G'AATT_C
Cuts at: 2490 3688 4066 2490
Size: 1198 378 4803
Fragments arranged by size:
4803 1198 378
EcoRV GAT'ATC
Cuts at: 3313 3313
Size: 6379
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3041 3085 3041
Size: 44 6335
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 2674 5526 1678
Size: 996 2852 2531
Fragments arranged by size:
2852 2531 996
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2782 2814 3632 3664 5020
Size: 32 135 32 1210 32 818 32 1356
Cuts at: 5020 5052 5094 5126 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 1210 818 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3723 3969 669
Size: 988 281 1785 246 3079
Fragments arranged by size:
3079 1785 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6379
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 2658 3704 2658
Size: 1046 5333
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 2560 6255 2560
Size: 3695 2684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5493 5677 1305
Size: 4188 184 2007
Fragments arranged by size:
4188 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4380
Fragments arranged by size:
4380 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2603 4337 4696 4855 518
Size: 648 1437 1734 359 159 2042
Fragments arranged by size:
2042 1734 1437 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3795 4472 5620 5865 1606
Size: 2189 677 1148 245 2120
Fragments arranged by size:
2189 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6379
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 3737 5282 1132
Size: 97 2508 1545 2229
Fragments arranged by size:
2508 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 5278 5278
Size: 6379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6379
PfoI T'CCnGG_A
Cuts at: 2485 2485
Size: 6379
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2643 4448 4480 5961 5993 2611
Size: 32 1805 32 1481 32 2997
Fragments arranged by size:
2997 1805 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2077
Size: 6379
PshAI GACnn'nnGTC
Cuts at: 1613 2162 2186 1613
Size: 549 24 5806
Fragments arranged by size:
5806 549 24
PsiI TTA'TAA
Cuts at: 2697 3949 2697
Size: 1252 5127
PspOMI G'GGCC_C
Cuts at: 3427 3427
Size: 6379
PstI C_TGCA'G
Cuts at: 830 3731 830
Size: 2901 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
SacI G_AGCT'C
Cuts at: 3153 3153
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SapI GCTCTTCn'nnn_
Cuts at: 2680 2680
Size: 6379
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 2644 4627 6339 2644
Size: 1983 1712 2684
Fragments arranged by size:
2684 1983 1712
SfcI C'TryA_G
Cuts at: 826 1045 2722 2985 3635 3727 4862 5543 5734
Size: 219 1677 263 650 92 1135 681 191
Cuts at: 5734 826
Size: 1471
Fragments arranged by size:
1677 1471 1135 681 650 263 219 191
92
SfiI GGCCn_nnn'nGGCC
Cuts at: 3117 3117
Size: 6379
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3359 4483 5384 5646 820
Size: 197 926 1416 1124 901 262 1553
Fragments arranged by size:
1553 1416 1124 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2004 3818 513
Size: 552 939 1814 3074
Fragments arranged by size:
3074 1814 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4406 4423 4576 4761 5206 325
Size: 1255 2826 17 153 185 445 1498
Fragments arranged by size:
2826 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 2642 3038
Size: 274 80 33 51 1443 552 113 396
Cuts at: 3038 3401 4625 6337 96
Size: 363 1224 1712 138
Fragments arranged by size:
1712 1443 1224 552 396 363 274 138
113 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 3339 3371 6339 6371 2022
Size: 32 1285 32 2968 32 2030
Fragments arranged by size:
2968 2030 1285 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 2481 2547 4508 2481
Size: 66 1961 4352
Fragments arranged by size:
4352 1961 66
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfoI
SrfI SwaI XcmI