Restriction map for
pFN21AE6436
(Circular) (Six-base) MAPSORT of: pFN21AE6436.seq Check: 4763 from: 1 to: 6085
pFN21AE6436 6085 bp
With 182 enzymes: SgfI *
May 20, 2014 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2553 2553
Size: 6085
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2038 2093 2195 2258 4148 5532 5800 1632
Size: 406 55 102 63 1890 1384 268 1917
Fragments arranged by size:
1917 1890 1384 406 268 102 63 55
AfeI AGC'GCT
Cuts at: 3255 3255
Size: 6085
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 3133 4984 5961 1203
Size: 1930 1851 977 1327
Fragments arranged by size:
1930 1851 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 2448 1166
Size: 1282 4803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2561 1184
Size: 1377 4708
AlwNI CAG_nnn'CTG
Cuts at: 2398 2610 3055 3828 5400 2398
Size: 212 445 773 1572 3083
Fragments arranged by size:
3083 1572 773 445 212
ApaI G_GGCC'C
Cuts at: 3080 3359 3080
Size: 279 5806
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 3394 3620 3772 5881 1493
Size: 1901 226 152 2109 1697
Fragments arranged by size:
2109 1901 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2061 3237 3410 1870
Size: 73 118 1176 173 4545
Fragments arranged by size:
4545 1176 173 118 73
BamHI G'GATC_C
Cuts at: 2388 3415 3782 2388
Size: 1027 367 4691
Fragments arranged by size:
4691 1027 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2672 3167 3406 4861
Size: 325 217 627 357 528 495 239 1455
Cuts at: 4861 618
Size: 1842
Fragments arranged by size:
1842 1455 627 528 495 357 325 239
217
BanII G_rGCy'C
Cuts at: 1391 3080 3359 3404 1391
Size: 1689 279 45 4072
Fragments arranged by size:
4072 1689 279 45
BbeI G_GCGC'C
Cuts at: 3171 3171
Size: 6085
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3355 928
Size: 971 1456 3658
Fragments arranged by size:
3658 1456 971
BbvCI CC'TCA_GC
Cuts at: 3108 3108
Size: 6085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2102 2136 4276 4310 1903
Size: 34 165 34 2140 34 3678
Fragments arranged by size:
3678 2140 165 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 2769 1548
Size: 1221 4864
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2282 3075
Size: 107 122 71 631 605 427 183 793
Cuts at: 3075 4696 136
Size: 1621 1525
Fragments arranged by size:
1621 1525 793 631 605 427 183 122
107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 2399 2826 3502 2399
Size: 427 676 4982
Fragments arranged by size:
4982 676 427
Bme1580I G_kGCm'C
Cuts at: 1790 2675 3080 3359 4146 5302 1790
Size: 885 405 279 787 1156 2573
Fragments arranged by size:
2573 1156 885 787 405 279
BmgBI CAC'GTC
Cuts at: 1318 3295 1318
Size: 1977 4108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2442 3816 4774
Size: 416 53 83 399 24 1025 1374 958
Cuts at: 4774 442
Size: 1753
Fragments arranged by size:
1753 1374 1025 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4745 1239
Size: 3506 2579
Bpu10I CC'TnA_GC
Cuts at: 2495 2504 3108 2495
Size: 9 604 5472
Fragments arranged by size:
5472 604 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4210 5075 5373 4210
Size: 865 298 4922
Fragments arranged by size:
4922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2058 4748 882
Size: 617 559 2690 2219
Fragments arranged by size:
2690 2219 617 559
BsaAI yAC'GTr
Cuts at: 493 1204 2845 3136 3876 493
Size: 711 1641 291 740 2702
Fragments arranged by size:
2702 1641 740 711 291
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2649 3168 3892 4274
Size: 53 83 186 936 1116 519 724 382
Cuts at: 4274 4909 275
Size: 635 1451
Fragments arranged by size:
1451 1116 936 724 635 519 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2474 2504 1178
Size: 30 165 30 1071 30 4759
Fragments arranged by size:
4759 1071 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2933 1186
Size: 874 873 4338
Fragments arranged by size:
4338 874 873
BseYI C'CCAG_C
Cuts at: 2250 2254 2260 2680 2830 3327 3370 5288 5784
Size: 4 6 420 150 497 43 1918 496
Cuts at: 5784 2250
Size: 2551
Fragments arranged by size:
2551 1918 497 496 420 150 43 6
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5512
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3535 4296 5324 1540
Size: 275 186 1534 761 1028 2301
Fragments arranged by size:
2301 1534 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4146 4231 5302 4146
Size: 85 1071 4929
Fragments arranged by size:
4929 1071 85
BsmI GAATG_Cn'
Cuts at: 3596 3689 3596
Size: 93 5992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2675 3080 3359 3404 4146 4231 5302
Size: 399 885 405 279 45 742 85 1071
Cuts at: 5302 1391
Size: 2174
Fragments arranged by size:
2174 1071 885 742 405 399 279 85
45
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3974 5704 3974
Size: 1730 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2553 3440 844
Size: 1232 477 887 3489
Fragments arranged by size:
3489 1232 887 477
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 1394
Size: 429 2149 3507
Fragments arranged by size:
3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2469 2877 4580 4754 65
Size: 1183 1221 408 1703 174 1396
Fragments arranged by size:
1703 1396 1221 1183 408 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3057 96
Size: 2961 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 2187 2657 4145 5157 2187
Size: 470 1488 1012 3115
Fragments arranged by size:
3115 1488 1012 470
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstXI CCAn_nnnn'nTGG
Cuts at: 2309 2309
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 2388 2979 3415 3782 4167 4184 5636 5722
Size: 416 591 436 367 385 17 1452 86
Cuts at: 5722 5734 6080 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 591 436 416 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2622 2622
Size: 6085
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 3042 513
Size: 552 939 102 936 3556
Fragments arranged by size:
3556 939 936 552 102
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2626 487
Size: 683 56 1400 3946
Fragments arranged by size:
3946 1400 683 56
BtsI GCAGTG_nn'
Cuts at: 2611 3601 4395 4415 2611
Size: 990 794 20 4281
Fragments arranged by size:
4281 990 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DraIII CAC_nnn'GTG
Cuts at: 2572 3348 2572
Size: 776 5309
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3149 5092 809
Size: 507 1833 1943 1802
Fragments arranged by size:
1943 1833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050
Size: 54 1425 50 275 18 1702 889 1629
Cuts at: 6050 8
Size: 43
Fragments arranged by size:
1702 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2748 4015 1353
Size: 1395 1267 3423
Fragments arranged by size:
3423 1395 1267
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2093 2195 2258 4148 4745 5532
Size: 393 406 55 102 63 1890 597 787
Cuts at: 5532 5800 1239
Size: 268 1524
Fragments arranged by size:
1890 1524 787 597 406 393 268 102
63 55
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2963 2072
Size: 891 5194
EcoO109I rG'GnC_Cy
Cuts at: 2091 3076 3112 3204 3356 2091
Size: 985 36 92 152 4820
Fragments arranged by size:
4820 985 152 92 36
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 2839 3171 3257 5232 1678
Size: 1161 332 86 1975 2531
Fragments arranged by size:
2531 1975 1161 332 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2474 2506 4726 4758 4800
Size: 32 135 32 902 32 2220 32 42
Cuts at: 4800 4832 1373
Size: 32 2626
Fragments arranged by size:
2626 2220 902 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3675 669
Size: 988 281 1491 246 3079
Fragments arranged by size:
3079 1491 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KasI G'GCGC_C
Cuts at: 3167 3167
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5199 5383 1305
Size: 3894 184 2007
Fragments arranged by size:
3894 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4263
Fragments arranged by size:
4263 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2448 2961 4043 4402 4561 518
Size: 648 1282 513 1082 359 159 2042
Fragments arranged by size:
2042 1282 1082 648 513 359 159
MspA1I CmG'CkG
Cuts at: 1606 2254 2395 3268 3501 4178 5326 5571 1606
Size: 648 141 873 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 873 677 648 245 233 141
NarI GG'CG_CC
Cuts at: 3168 3168
Size: 6085
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 3443 4988 1132
Size: 97 2214 1545 2229
Fragments arranged by size:
2229 2214 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3204 3204
Size: 6085
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 3076 3355 3076
Size: 279 5806
PstI C_TGCA'G
Cuts at: 830 2540 3030 3280 3437 830
Size: 1710 490 250 157 3478
Fragments arranged by size:
3478 1710 490 250 157
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2254 2395 3268 2254
Size: 141 873 5071
Fragments arranged by size:
5071 873 141
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SanDI GG'GwC_CC
Cuts at: 3204 3204
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2536 3026 3276 3433 4568 5249 5440
Size: 219 1491 490 250 157 1135 681 191
Cuts at: 5440 826
Size: 1471
Fragments arranged by size:
1491 1471 1135 681 490 250 219 191
157
SfiI GGCCn_nnn'nGGCC
Cuts at: 2099 2099
Size: 6085
SfoI GGC'GCC
Cuts at: 3169 3169
Size: 6085
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3239 3412 1872
Size: 1367 173 4545
Fragments arranged by size:
4545 1367 173
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4189 5090 5352 820
Size: 197 926 2246 901 262 1553
Fragments arranged by size:
2246 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 4009 5
Size: 47 3957 2081
Fragments arranged by size:
3957 2081 47
StuI AGG'CCT
Cuts at: 1517 2627 3212 1517
Size: 1110 585 4390
Fragments arranged by size:
4390 1110 585
StyI C'CwwG_G
Cuts at: 513 1065 2004 2707 2781 3524 513
Size: 552 939 703 74 743 3074
Fragments arranged by size:
3074 939 743 703 552 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2132 2573 4112 4129 4282 4467 4912
Size: 1255 552 441 1539 17 153 185 445
Cuts at: 4912 325
Size: 1498
Fragments arranged by size:
1539 1498 1255 552 445 441 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2790 3057 4331
Size: 274 80 33 51 1443 813 267 1274
Cuts at: 4331 6043 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1274 813 274 267 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3290 3322 6045 6077 3290
Size: 32 2723 32 3298
Fragments arranged by size:
3298 2723 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2455 3238 2455
Size: 783 5302
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3237 3410 1870
Size: 1367 173 4545
Fragments arranged by size:
4545 1367 173
XmnI GAAnn'nnTTC
Cuts at: 4214 4214
Size: 6085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacI SacII SapI SexAI SrfI SwaI