Restriction map for
pFN21AE6433
(Circular) (Six-base) MAPSORT of: pFN21AE6433.seq Check: 6027 from: 1 to: 6046
pFN21AE6433 6046 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2515 2515
Size: 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3856 278
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 3163 3389 1937
Size: 537 689 226 4594
Fragments arranged by size:
4594 689 537 226
Acc65I G'GTAC_C
Cuts at: 2274 3367 2274
Size: 1093 4953
AclI AA'CG_TT
Cuts at: 1563 4173 4546 1563
Size: 2610 373 3063
Fragments arranged by size:
3063 2610 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2858 4109 5493 5761 1632
Size: 1226 1251 1384 268 1917
Fragments arranged by size:
1917 1384 1251 1226 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5849
AflIII A'CryG_T
Cuts at: 1203 2211 3219 4945 5922 1203
Size: 1008 1008 1726 977 1327
Fragments arranged by size:
1726 1327 1008 1008 977
AgeI A'CCGG_T
Cuts at: 2277 4877 2277
Size: 2600 3446
AhdI GACnn_n'nnGTC
Cuts at: 2470 4775 2470
Size: 2305 3741
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6046
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6046
AlwNI CAG_nnn'CTG
Cuts at: 2314 2995 3789 5361 2314
Size: 681 794 1572 2999
Fragments arranged by size:
2999 1572 794 681
ApaI G_GGCC'C
Cuts at: 2347 2347
Size: 6046
ApaLI G'TGCA_C
Cuts at: 3004 4103 5259 3004
Size: 1099 1156 3791
Fragments arranged by size:
3791 1156 1099
ApoI r'AATT_y
Cuts at: 1493 2506 2681 2742 3083 3108 3355 3581 3733
Size: 1013 175 61 341 25 247 226 152
Cuts at: 3733 5842 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1013 341 247 226 175 152
61 25
AseI AT'TA_AT
Cuts at: 160 1032 4600 160
Size: 872 3568 1606
Fragments arranged by size:
3568 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2349 3371 1870
Size: 73 306 100 1022 4545
Fragments arranged by size:
4545 1022 306 100 73
AvrII C'CTAG_G
Cuts at: 3113 3113
Size: 6046
BamHI G'GATC_C
Cuts at: 3376 3743 3376
Size: 367 5679
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2274 2336 3367 4822
Size: 325 217 627 363 124 62 1031 1455
Cuts at: 4822 618
Size: 1842
Fragments arranged by size:
1842 1455 1031 627 363 325 217 124
62
BanII G_rGCy'C
Cuts at: 1391 2347 3365 1391
Size: 956 1018 4072
Fragments arranged by size:
4072 1018 956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2809 3068 3343 928
Size: 971 910 259 275 3631
Fragments arranged by size:
3631 971 910 275 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4237 4271 1903
Size: 34 2300 34 3678
Fragments arranged by size:
3678 2300 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2658 3938 5148 2658
Size: 1280 1210 3556
Fragments arranged by size:
3556 1280 1210
BclI T'GATC_A
Cuts at: 1548 2305 1548
Size: 757 5289
BfrBI ATG'CAT
Cuts at: 3145 3145
Size: 6046
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4657 136
Size: 107 122 71 631 605 2985 1525
Fragments arranged by size:
2985 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6041 6041
Size: 6046
BlpI GC'TnA_GC
Cuts at: 3463 3463
Size: 6046
Bme1580I G_kGCm'C
Cuts at: 1790 2347 2855 3008 3184 4107 5263 1790
Size: 557 508 153 176 923 1156 2573
Fragments arranged by size:
2573 1156 923 557 508 176 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6046
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3049 3777 4735
Size: 416 53 83 399 24 1632 728 958
Cuts at: 4735 442
Size: 1753
Fragments arranged by size:
1753 1632 958 728 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2098 3150 4706 1239
Size: 859 1052 1556 2579
Fragments arranged by size:
2579 1556 1052 859
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4171 5036 5334 4171
Size: 865 298 4883
Fragments arranged by size:
4883 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2209 2592 4709 882
Size: 617 710 383 2117 2219
Fragments arranged by size:
2219 2117 710 617 383
BsaAI yAC'GTr
Cuts at: 493 1204 3837 493
Size: 711 2633 2702
Fragments arranged by size:
2702 2633 711
BsaBI GATnn'nnATC
Cuts at: 6040 6040
Size: 6046
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2133 3853 4235 4870
Size: 53 83 186 936 600 1720 382 635
Cuts at: 4870 275
Size: 1451
Fragments arranged by size:
1720 1451 936 635 600 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2277 2515 4479 4877 5151 5298 5709 2277
Size: 238 1964 398 274 147 411 2614
Fragments arranged by size:
2614 1964 411 398 274 238 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5821
Fragments arranged by size:
5821 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6046
BseYI C'CCAG_C
Cuts at: 2244 5249 5745 2244
Size: 3005 496 2545
Fragments arranged by size:
3005 2545 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5473
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2161 2478 3496 4257 5285 1540
Size: 275 186 160 317 1018 761 1028 2301
Fragments arranged by size:
2301 1028 1018 761 317 275 186 160
BsiHKAI G_wGCw'C
Cuts at: 2955 3008 4107 4192 5263 2955
Size: 53 1099 85 1071 3738
Fragments arranged by size:
3738 1099 1071 85 53
BsmI GAATG_Cn'
Cuts at: 2544 2617 2986 3557 3650 2544
Size: 73 369 571 93 4940
Fragments arranged by size:
4940 571 369 93 73
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2347 2855 2955 3008 3184 3365 4107
Size: 399 557 508 100 53 176 181 742
Cuts at: 4107 4192 5263 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 557 508 399 181 176
100 85 53
BspEI T'CCGG_A
Cuts at: 5709 5709
Size: 6046
BspHI T'CATG_A
Cuts at: 3935 5665 3935
Size: 1730 4316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2515 2610 3334 3401 844
Size: 1671 95 724 67 3489
Fragments arranged by size:
3489 1671 724 95 67
BsrBI CCG'CTC
Cuts at: 1394 1823 3933 1394
Size: 429 2110 3507
Fragments arranged by size:
3507 2110 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2092 4541 4715 65
Size: 1183 844 2449 174 1396
Fragments arranged by size:
2449 1396 1183 844 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2153 2277 2382 4690 4877 1700
Size: 110 343 124 105 2308 187 2869
Fragments arranged by size:
2869 2308 343 187 124 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6046
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6046
BssSI C'ACGA_G
Cuts at: 2048 4106 5118 2048
Size: 2058 1012 2976
Fragments arranged by size:
2976 2058 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6046
BstBI TT'CG_AA
Cuts at: 2419 4850 2419
Size: 2431 3615
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6046
BstXI CCAn_nnnn'nTGG
Cuts at: 3087 3087
Size: 6046
BstYI r'GATC_y
Cuts at: 1972 3117 3376 3743 4128 4145 5597 5683 5695
Size: 1145 259 367 385 17 1452 86 12
Cuts at: 5695 6041 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1145 385 367 346 259 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2315 2315
Size: 6046
BtgI C'CryG_G
Cuts at: 513 1065 2161 513
Size: 552 1096 4398
Fragments arranged by size:
4398 1096 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5307
Fragments arranged by size:
5307 683 56
BtsI GCAGTG_nn'
Cuts at: 3562 4356 4376 3562
Size: 794 20 5232
Fragments arranged by size:
5232 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6046
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6011
DraI TTT'AAA
Cuts at: 3350 3350
Size: 6046
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5053 809
Size: 507 3737 1802
Fragments arranged by size:
3737 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2158 2662
Size: 54 1425 50 275 18 225 103 504
Cuts at: 2662 3493 4382 6011 8
Size: 831 889 1629 43
Fragments arranged by size:
1629 1425 889 831 504 275 225 103
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2158 3493 1537
Size: 275 346 1335 4090
Fragments arranged by size:
4090 1335 346 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2775 3057 3976 1353
Size: 1422 282 919 3423
Fragments arranged by size:
3423 1422 919 282
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 4633 5007 5153 387
Size: 1064 138 681 2363 374 146 1280
Fragments arranged by size:
2363 1280 1064 681 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2098 2858 3150 4109 4706 5493 5761
Size: 393 466 760 292 959 597 787 268
Cuts at: 5761 1239
Size: 1524
Fragments arranged by size:
1524 959 787 760 597 466 393 292
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2780 2780
Size: 6046
EcoO109I rG'GnC_Cy
Cuts at: 2110 2344 2880 2110
Size: 234 536 5276
Fragments arranged by size:
5276 536 234
EcoRI G'AATT_C
Cuts at: 3355 3733 3355
Size: 378 5668
FspI TGC'GCA
Cuts at: 4552 4552
Size: 6046
HaeII r_GCGC'y
Cuts at: 1678 5193 1678
Size: 3515 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2162 2194 2456 2488 2683
Size: 32 135 32 590 32 262 32 195
Cuts at: 2683 2715 2855 2887 4687 4719 4761 4793 1373
Size: 32 140 32 1800 32 42 32 2626
Fragments arranged by size:
2626 1800 590 262 195 140 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 3390 3636 669
Size: 988 281 537 915 246 3079
Fragments arranged by size:
3079 988 915 537 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6046
HpaI GTT'AAC
Cuts at: 3636 3636
Size: 6046
KpnI G_GTAC'C
Cuts at: 2278 3371 2278
Size: 1093 4953
MfeI C'AATT_G
Cuts at: 3645 3645
Size: 6046
MluI A'CGCG_T
Cuts at: 5922 5922
Size: 6046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2232 5160 5344 1305
Size: 927 2928 184 2007
Fragments arranged by size:
2928 2007 927 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4224
Fragments arranged by size:
4224 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4004 4363 4522 518
Size: 648 2838 359 159 2042
Fragments arranged by size:
2838 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2163 2296 2491 3462 4139 5287 5532 1606
Size: 557 133 195 971 677 1148 245 2120
Fragments arranged by size:
2120 1148 971 677 557 245 195 133
NaeI GCC'GGC
Cuts at: 2155 2155
Size: 6046
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5494
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6046
NgoMIV G'CCGG_C
Cuts at: 2153 2153
Size: 6046
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6046
NotI GC'GGCC_GC
Cuts at: 2158 3493 2158
Size: 1335 4711
NsiI A_TGCA'T
Cuts at: 3147 3147
Size: 6046
NspI r_CATG'y
Cuts at: 1132 1229 3223 3404 4949 1132
Size: 97 1994 181 1545 2229
Fragments arranged by size:
2229 1994 1545 181 97
PasI CC'CwG_GG
Cuts at: 1348 2428 2875 1348
Size: 1080 447 4519
Fragments arranged by size:
4519 1080 447
PciI A'CATG_T
Cuts at: 3219 4945 3219
Size: 1726 4320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6046
PmeI GTTT'AAAC
Cuts at: 3350 3350
Size: 6046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4115 4147 5628 5660 4115
Size: 32 1481 32 4501
Fragments arranged by size:
4501 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2880 2880
Size: 6046
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6046
PsiI TTA'TAA
Cuts at: 3036 3616 3036
Size: 580 5466
PspOMI G'GGCC_C
Cuts at: 2343 2343
Size: 6046
PstI C_TGCA'G
Cuts at: 830 2526 3345 3398 830
Size: 1696 819 53 3478
Fragments arranged by size:
3478 1696 819 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6046
PvuII CAG'CTG
Cuts at: 2296 2491 2296
Size: 195 5851
SacII CC_GC'GG
Cuts at: 2164 2164
Size: 6046
SalI G'TCGA_C
Cuts at: 1936 2473 3388 1936
Size: 537 915 4594
Fragments arranged by size:
4594 915 537
SbfI CC_TGCA'GG
Cuts at: 3398 3398
Size: 6046
ScaI AGT'ACT
Cuts at: 4294 6006 4294
Size: 1712 4334
SfcI C'TryA_G
Cuts at: 826 1045 2522 2884 3160 3341 3394 4529 5210
Size: 219 1477 362 276 181 53 1135 681
Cuts at: 5210 5401 826
Size: 191 1471
Fragments arranged by size:
1477 1471 1135 681 362 276 219 191
181 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6046
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6046
SmaI CCC'GGG
Cuts at: 1872 2351 3373 1872
Size: 479 1022 4545
Fragments arranged by size:
4545 1022 479
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4150 5051 5313 820
Size: 197 926 2207 901 262 1553
Fragments arranged by size:
2207 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3837 493
Size: 3344 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6046
SphI G_CATG'C
Cuts at: 1132 3404 1132
Size: 2272 3774
SspI AAT'ATT
Cuts at: 5 52 3970 5
Size: 47 3918 2081
Fragments arranged by size:
3918 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6046
StyI C'CwwG_G
Cuts at: 513 1065 3113 3485 513
Size: 552 2048 372 3074
Fragments arranged by size:
3074 2048 552 372
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2492 4073 4090 4243 4428 4873 325
Size: 1255 912 1581 17 153 185 445 1498
Fragments arranged by size:
1581 1498 1255 912 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3020 4292 6004
Size: 274 80 33 51 1443 1043 1272 1712
Cuts at: 6004 96
Size: 138
Fragments arranged by size:
1712 1443 1272 1043 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 6006 6038 2872
Size: 32 3102 32 2880
Fragments arranged by size:
3102 2880 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2441 1316
Size: 1125 4921
XbaI T'CTAG_A
Cuts at: 3382 3382
Size: 6046
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6046
XmaI C'CCGG_G
Cuts at: 1870 2349 3371 1870
Size: 479 1022 4545
Fragments arranged by size:
4545 1022 479
XmnI GAAnn'nnTTC
Cuts at: 4175 4175
Size: 6046
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3854 276
Size: 53 83 186 936 2320 2468
Fragments arranged by size:
2468 2320 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspXI
PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI