Restriction map for
pFN21AE6431
(Circular) (Six-base) MAPSORT of: pFN21AE6431.seq Check: 7465 from: 1 to: 6085
pFN21AE6431 6085 bp
With 182 enzymes: SgfI *
March 18, 2015 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2357 3895 278
Size: 53 83 186 936 821 1538 2468
Fragments arranged by size:
2468 1538 936 821 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 1937
Size: 1491 4594
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2208 2552 2712 2723 3169 4148 5532 5800
Size: 576 344 160 11 446 979 1384 268
Cuts at: 5800 1632
Size: 1917
Fragments arranged by size:
1917 1384 979 576 446 344 268 160
11
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 3017 1166
Size: 1851 4234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 2749 3828 5400 2749
Size: 1079 1572 3434
Fragments arranged by size:
3434 1572 1079
ApaI G_GGCC'C
Cuts at: 2071 3374 2071
Size: 1303 4782
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 2716 2867 2888 3394 3620 3772 5881 1493
Size: 1223 151 21 506 226 152 2109 1697
Fragments arranged by size:
2109 1697 1223 506 226 152 151 21
AseI AT'TA_AT
Cuts at: 160 1032 4639 160
Size: 872 3607 1606
Fragments arranged by size:
3607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 2259 3410 1870
Size: 73 316 1151 4545
Fragments arranged by size:
4545 1151 316 73
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3082 3406 4861 618
Size: 325 217 627 1295 324 1455 1842
Fragments arranged by size:
1842 1455 1295 627 325 324 217
BanII G_rGCy'C
Cuts at: 1391 2071 3099 3374 3404 1391
Size: 680 1028 275 30 4072
Fragments arranged by size:
4072 1028 680 275 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 928
Size: 971 801 4313
Fragments arranged by size:
4313 971 801
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 2814 1548
Size: 1266 4819
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 3502 3502
Size: 6085
Bme1580I G_kGCm'C
Cuts at: 1790 2071 3374 4146 5302 1790
Size: 281 1303 772 1156 2573
Fragments arranged by size:
2573 1303 1156 772 281
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2154 3816 4774
Size: 416 53 83 399 24 737 1662 958
Cuts at: 4774 442
Size: 1753
Fragments arranged by size:
1753 1662 958 737 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4745 1239
Size: 3506 2579
Bpu10I CC'TnA_GC
Cuts at: 2661 3072 2661
Size: 411 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3135 4210 5075 5373 3135
Size: 1075 865 298 3847
Fragments arranged by size:
3847 1075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 882
Size: 617 3249 2219
Fragments arranged by size:
3249 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3876 493
Size: 711 2672 2702
Fragments arranged by size:
2702 2672 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2354 3892 4274
Size: 53 83 186 936 476 345 1538 382
Cuts at: 4274 4909 275
Size: 635 1451
Fragments arranged by size:
1538 1451 936 635 476 382 345 186
83 53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5860
Fragments arranged by size:
5860 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3053 3251 1186
Size: 1867 198 4020
Fragments arranged by size:
4020 1867 198
BseYI C'CCAG_C
Cuts at: 2296 3348 5288 5784 2296
Size: 1052 1940 496 2597
Fragments arranged by size:
2597 1940 1052 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5512
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2117 3535 4296 5324 1540
Size: 275 186 116 1418 761 1028 2301
Fragments arranged by size:
2301 1418 1028 761 275 186 116
BsiHKAI G_wGCw'C
Cuts at: 3099 4146 4231 5302 3099
Size: 1047 85 1071 3882
Fragments arranged by size:
3882 1071 1047 85
BsmI GAATG_Cn'
Cuts at: 3596 3689 3596
Size: 93 5992
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 3099 3374 3404 4146 4231 5302
Size: 399 281 1028 275 30 742 85 1071
Cuts at: 5302 1391
Size: 2174
Fragments arranged by size:
2174 1071 1028 742 399 281 275 85
30
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3974 5704 3974
Size: 1730 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2074 3972 1394
Size: 429 251 1898 3507
Fragments arranged by size:
3507 1898 429 251
BsrDI GCAATG_nn'
Cuts at: 65 1248 4580 4754 65
Size: 1183 3332 174 1396
Fragments arranged by size:
3332 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 2135 2266 2577 4145 5157 2135
Size: 131 311 1568 1012 3063
Fragments arranged by size:
3063 1568 1012 311 131
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstXI CCAn_nnnn'nTGG
Cuts at: 3202 3202
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 3415 3782 4167 4184 5636 5722 5734 6080
Size: 1443 367 385 17 1452 86 12 346
Cuts at: 6080 1972
Size: 1977
Fragments arranged by size:
1977 1452 1443 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 3066 3601 4395 4415 3066
Size: 535 794 20 4736
Fragments arranged by size:
4736 794 535 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3280 3389 3280
Size: 109 5976
DraIII CAC_nnn'GTG
Cuts at: 2140 3233 2140
Size: 1093 4992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050
Size: 54 1425 50 275 18 1702 889 1629
Cuts at: 6050 8
Size: 43
Fragments arranged by size:
1702 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4015 1353
Size: 2662 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3097 3097
Size: 6085
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2552 2712 2723 3169 4148 4745
Size: 393 576 344 160 11 446 979 597
Cuts at: 4745 5532 5800 1239
Size: 787 268 1524
Fragments arranged by size:
1524 979 787 597 576 446 393 344
268 160 11
EcoO109I rG'GnC_Cy
Cuts at: 2068 2977 3046 2068
Size: 909 69 5107
Fragments arranged by size:
5107 909 69
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
EcoRV GAT'ATC
Cuts at: 3058 3058
Size: 6085
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4726 4758 4800 4832 1373
Size: 32 135 32 3154 32 42 32 2626
Fragments arranged by size:
3154 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3675 669
Size: 988 281 1491 246 3079
Fragments arranged by size:
3079 1491 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6085
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 2218 3684 2218
Size: 1466 4619
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2004 5199 5383 1305
Size: 699 3195 184 2007
Fragments arranged by size:
3195 2007 699 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4263
Fragments arranged by size:
4263 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3017 4043 4402 4561 518
Size: 648 1851 1026 359 159 2042
Fragments arranged by size:
2042 1851 1026 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2902 3501 4178 5326 5571 1606
Size: 1296 599 677 1148 245 2120
Fragments arranged by size:
2120 1296 1148 677 599 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4594
Fragments arranged by size:
4594 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2245 2337 3356 3443 4988 1132
Size: 97 1016 92 1019 87 1545 2229
Fragments arranged by size:
2229 1545 1019 1016 97 92 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2582 3355 1108
Size: 1474 773 3838
Fragments arranged by size:
3838 1474 773
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2977 3046 2977
Size: 69 6016
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 3655 3655
Size: 6085
PspOMI G'GGCC_C
Cuts at: 2067 3370 2067
Size: 1303 4782
PstI C_TGCA'G
Cuts at: 830 2022 3437 830
Size: 1192 1415 3478
Fragments arranged by size:
3478 1415 1192
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
PvuII CAG'CTG
Cuts at: 2902 2902
Size: 6085
RsrII CG'GwC_CG
Cuts at: 2026 2026
Size: 6085
SacI G_AGCT'C
Cuts at: 3099 3099
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SanDI GG'GwC_CC
Cuts at: 3046 3046
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 2018 2385 3433 4568 5249 5440 826
Size: 219 973 367 1048 1135 681 191 1471
Fragments arranged by size:
1471 1135 1048 973 681 367 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3150 4189 5090 5352 820
Size: 197 926 1207 1039 901 262 1553
Fragments arranged by size:
1553 1207 1039 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6085
SphI G_CATG'C
Cuts at: 1132 2245 3443 1132
Size: 1113 1198 3774
Fragments arranged by size:
3774 1198 1113
SspI AAT'ATT
Cuts at: 5 52 2453 4009 5
Size: 47 2401 1556 2081
Fragments arranged by size:
2401 2081 1556 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 2004 3524 513
Size: 552 939 1520 3074
Fragments arranged by size:
3074 1520 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3029 3362 4112 4129 4282 4467 4912
Size: 1255 1449 333 750 17 153 185 445
Cuts at: 4912 325
Size: 1498
Fragments arranged by size:
1498 1449 1255 750 445 333 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4331 6043 96
Size: 274 80 33 51 1443 2354 1712 138
Fragments arranged by size:
2354 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6045 6077 6045
Size: 32 6053
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6085
XbaI T'CTAG_A
Cuts at: 2357 3421 2357
Size: 1064 5021
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 4214 4214
Size: 6085
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2355 3893 276
Size: 53 83 186 936 821 1538 2468
Fragments arranged by size:
2468 1538 936 821 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI SacII SapI SexAI SfiI
SfoI SrfI SwaI XcmI